Pubmed abstracts for Protein-Protein Interaction search result :


Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.64513447
The identified Dictyostelium PLC , DdPLC , encodes a protein with a calculated molecular mass of 91 kDa . 0.64513447^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.50807311
A 2178 bp protein coding region of the T . cruzi PLC gene , composed from cDNA and genomic clones , encodes a putative PLC with a calculated molecular mass of 82 , 032 Da and an isoelectric point of 5 . 93 . 0.50807311^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
The approx . molecular mass of the HSPG was 200 kDa , as measured by gel electrophoresis and gel chromatography . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
Periodontal fibroblasts produce six major proteoglycans : versican , a high molecular mass chondroitin sulphate proteoglycan ( CSPG ) ; decorin , a dermatan sulphate proteoglycan ( DSPG ) ; a membrane associated heparan sulphate proteoglycan ( HSPG ) ; two medium or matrix associated HSPGs ; and a 91 kDa membrane associated CSPG . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
PGs released from PC 12 cells into the culture medium were mostly CSPGs . ( 2 ) In the absence of NGF , the apparent molecular mass of HSPG prepared from PC 12 cells after 3 days of culture was in the range of 90 190 kDa for the intact form ( Kav = 0 . 38 on Sepharose CL 6B ) , 12 kDa for HS , and 61 kDa for the core protein . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
The low molecular weight PLC hydrolyzes both PI and PIP 2 , has a molecular mass of 58 kDa , requires the most calcium for full activation , and has a PI pH profile that shifts slightly with calcium concentration . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
The activities of PLD and PLC were examined in purified mast cells by quantitating the mass of the water soluble hydrolysis products choline and phosphorylcholine , respectively . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
Insulin increased total DAG mass less than PLC in control muscles and did not affect DAG in denervated muscles . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
These results indicate that the increase in the mass of PA by CCh is due to immediate phosphorylation by DG kinase to PA , of the DG produced by phospholipase C ( PLC ) . ^^^ These results suggest that CCh activates both PLC and DG kinase and the resultant increase in the mass of PA contributes to the regulation of the sustained phase of CCh induced contraction which is related to Ca2+ influx . . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
In resealed ROS and washed ROS membranes , Ab 1109 recognized an additional protein of apparent molecular mass of 70 kDa not usually detectable in soluble extracts of ROS , suggesting the presence of at least two isozymes of PLC in ROS . . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
When released by plasmin , the growth factor is recovered from the incubation medium as a complex with the partly degraded high molecular mass HSPG . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
Proteoglycans binding to phenyl Sepharose had an estimated molecular mass of 400 500 kDa , and contained 60 % HSPG and 40 % chondroitin sulfate proteoglycan ( CSPG ) . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
Propranolol does not inhibit phosphoinositide specific PLC and yet it causes almost complete inhibition of the total DG mass accumulation in C5a stimulated neutrophils . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
Polyacrylamide gel electrophoresis of non reduced heparitinase digested membrane HSPG followed by Western blotting and immunostaining with Mab S 1 revealed a single band with apparent molecular mass of 64 kDa . ^^^ Fractionation of 125I labelled membrane HSPG by immuno affinity chromatography on immobilized Mab S 1 , followed by heparitinase digestion and polyacrylamide gel electrophoresis of the bound material , yielded a single labelled band with apparent molecular mass 64 kDa . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
When analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and autoradiography , core ( protein ) preparations , obtained by heparitinase digestion of 125I labeled HSPG fractions , yielded one major labeled band with apparent molecular mass of approximately 300 kDa . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
Cryosurgery with liquid nitrogen can be considered the surgery of choice for nonresectable PLC in patients without jaundice , ascites , and noncompensated liver function , and the whole tumor mass can be involved in the frozen area . . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
The deduced amino acid sequence of the cDNA accounted for 146 . 1 kDa of the molecular mass of the complete enzyme and showed 50 . 2 % sequence similarity to bovine brain PLC . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
Each of them encodes a distinct polypeptide with a calculated molecular mass of 138 , 225 ( PLC 1 ) and 85 , 840 ( PLC 3 ) . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
The amplified pelC cDNA was expressed in Pichia pastoris yielding a pectate lyase C ( PLC ) with a molecular mass of 26 . 0 kDa and containing carbohydrates . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
Tyrosine phosphorylation of PLC gamma 1 correlated with the time and concentration dependent decrease in the mass of membrane phosphatidylinositol 4 , 5 bisphosphate ( PIP 2 ) and the increase in the epithelial concentration of inositol 1 , 4 , 5 trisphosphate ( IP 3 ) . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
Preincubation with U 73 , 122 , however , totally abolished PTH stimulated increases in diglyceride mass , consistent with inhibition of PLC . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
ANG 2 was shown to increase the mass of three separate PA species , one of which apparently originated from DAG kinase action on PC phospholipase C ( PLC ) produced DAG , providing evidence for PC PLC activity . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
PL C is an acidic simple protein with molecular mass of 13 , 747 Da and consists of three cysteine rich domains with 51 63 % homology . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
Direct measurements of PIP 2 mass showed that the CapG effect on PLC gamma was not due primarily to an increase in the PIP 2 substrate concentration . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
A protein with apparent molecular mass of 130kD was recognized by an antibody against PLC beta 4 / norp A in both 400 mM KCl soluble and rhabdomal membrane fractions . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
Mass analysis of inositol phosphates demonstrated the presence of a broad spectrum of inositol phosphates in Dictyostelium , which was unaltered in the plc mutant . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
The plc gene contained 984 bp and coded for a mature protein with a calculated molecular mass of 34 , 107 Da . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
The apparent molecular mass of PLC 1 as determined by SDS / PAGE and gel filtration was 85 kDa . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
SDS / PAGE analysis of the PtdIns PLC releasable HSPG indicated a molecular mass of 250 kDa . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
Since the mass of PtdIns declines to a similar extent as [ 3H ] PtdIns during stimulation in the presence of guanine nucleotides and ATP , it is evident that both the labelled and unlabelled phosphoinositides are susceptible to hydrolysis by the relevant PLC . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
The CDSPG was resolved from the HSPG by molecular sieve chromatography , which indicated that the molecular mass of the HSPG was greater than 300 kDa , while that of the CDSPG was approximately 50 kDa . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
The new PLC was purified from a retinal particulate fraction to yield a preparation that contained a major protein band with an apparent molecular mass of 130 kDa on SDS polyacrylamide gels . ^^^ The cDNA encoded a putative polypeptide of 1176 amino acids , with a calculated molecular mass of 134 , 532 daltons , that contained the sequences of all 12 tryptic peptides of PLC beta 4 . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
The purified enzyme exhibited an apparent molecular mass of 152 kDa on SDS polyacrylamide gels , as compared with 150 and 140 kDa for PLC beta 1 and PLC beta 2 , respectively . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
Second , in contrast with phospholipase C ( PLC ) activation , DG mass accumulation is dependent on extracellular Ca2+ . ^^^ Similarly , DG mass accumulation is not attenuated by protein kinase C activation , which we have previously shown to inhibit the phosphoinositide specific PLC . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
The encoded polypeptide contains 772 amino acids ( calculated molecular mass , 88 , 966 daltons ) and is similar in primary structure to delta type PLC isozymes , with overall sequence identities of 45 % to PLC delta 1 , 72 % to PLC delta 2 , and 47 % to PLC delta 3 . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
The aim of the present study was to characterize the mechanisms of desensitization of the mu opioid induced inositol ( 1 , 4 , 5 ) triphosphate ( Ins ( 1 , 4 , 5 ) P 3 ) response , by use of a stereospecific radioreceptor mass assay . 2 . ( R+ ) Bay K 8644 ( 1 nM 10 microM ) dose dependently inhibited fentanyl induced Ins ( 1 , 4 , 5 ) P 3 formation , with an IC 50 of 28 . 5 nM , confirming our earlier observations that mu opioids open L type VSCCs , thus allowing Ca2+ influx to activate PLC . 3 . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
Incubation of cultured growth arrested rat mesangial cells with PGF2alpha ( 1 microM ) resulted in activation of a phosphatidyl inositol specific phospholipase C ( PI PLC ) assessed as increased generation of polyphosphates in myo [ 3H ] inositol labeled cells and as increased diacylglycerol ( DAG ) mass levels measured by a radioenzymatic assay . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
The open reading frame of this cDNA encodes a 1211 amino acid protein ( calculated molecular mass 139050 Da ) that contains amino acid sequences of 16 peptides sequenced from the turkey erythrocyte PLC . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
In addition , omega 3 and omega 6 fatty acid substituted cells showed similar increases in diacylglycerol mass in response to sublethal injury by C5b 9 , suggesting that omega 3 incorporation did not limit phospholipid ( PL ) hydrolysis by PLC . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
The molecular mass of the glycosaminoglycan chain from pronase digestion of HSPG was estimated to be 30 100 kDa . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
Subjects consumed a CHO ( 1 . 0 g CHO . kg body mass ( 1 ) ) or placebo treatment ( PLC ) , prior to and every 10 min ( 0 . 5 g CHO . kg body mass ( 1 ) ) during the IRT . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
PLC as well as oleate treatment concomitantly increased the TAG mass within the cell . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
Subjects consumed 1 . 0 g  kg body mass ( 1 ) CHO or an equal volume of placebo ( PLC ) 10 min prior to and 10 min following exercise . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
However , for total body mass , fat free mass , thigh volume , muscle strength , and myofibrillar protein , CRT and PLC exhibited significant increases after training when compared to CON ( P < 0 . 05 ) , whereas CRT was also significantly greater than PLC ( P < 0 . 05 ) . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
This is the first evidence of alterations in the proteoglycan expression pattern of versican , perlecan , biglycan and decorin which can be linked to the pathophysiological state of asthmatics proven by a combination of solid phase extraction by reversed phase and by peptide mass fingerprinting using matrix assisted laser desorption / ionization time of flight mass spectrometry . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
The subjects performed a first 100 KJ TEST ( RIDE 1 ) , ingested 0 . 7 g . ( kg body mass ) ( 1 ) of Gatorlode ( CHO ) or placebo ( PLC ) , rested for 60 minutes , and then performed a second 100 KJ TEST ( RIDE 2 ) . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
Eight subjects first performed exhausting exercise at 120 % peak oxygen uptake after ingesting either a placebo ( PLC ) or sodium citrate ( CIT ) at a dose of 0 . 5 g . kg 1 body mass to determine exhaustion time ( texh ) . ^^^ The CIT raised the rest [ citrate ] in blood [ PLC 0 . 11 ( SD 0 . 01 ) mmol . l 1 , CIT 0 . 34 ( SD 0 . 07 ) mmol . l 1 ] and in muscle [ PLC 0 . 78 ( SD 0 . 23 ) mmol . kg 1 dry mass , CIT 1 . 00 ( SD 0 . 21 ) mmol . kg 1 dry mass ] . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
In SOC rats the activity of phosphatidylcholine PLA 2 and PE PLA 2 , the activity of PLC , and the mass of COX were significantly greater in medullary tubules than in cortical tubules . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
Furthermore , Cvx stimulates a rapid tyrosyl phosphorylation of a 145 kDa molecular mass protein that was identified as PLC gamma 2 . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
SL PLC isoenzymes were examined in terms of protein mass and hydrolytic activity . ^^^ CHF resulted in a striking reduction ( to 6 17 % of controls ) of the mass and activity of gamma ( 1 ) and delta ( 1 ) isoforms in combination with a significant increase of both PLC beta ( 1 ) parameters . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
CONCLUSION : This suggests mass screening , especially continual screening every 6 months in fixed population can diagnose PLC early . . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
By using detection methods such as thin layer chromatography and mass spectrometry , PLC activity could not be detected in several strains of Legionella pneumophila . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
Subjects consumed 1 . 0 g 10 kg body mass ( 1 ) CHO or an equal volume of placebo ( PLC ) 10 min prior to and 10 min following exercise . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
Fractionation of the conditioned medium followed by mass spectral analysis identified one bioactive component as a C terminal fragment of the domain 5 of perlecan . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
PMA treated cells were shown to degrade 35S sulfated HSPG in endothelial extracellular matrix into fragments of an approximate molecular mass of 5 kDa . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
Thirty years ago , patients with PLC were often detected at relatively late stage , with a palpable mass or marked clinical symptoms and poor prognosis . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
Analysis of the 90 KD fragment by Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry ( MALDI TOF MS ) and Capillary LC nano Electrospray Ionization tandem MS ( LC nano ESI MS / MS ) showed that it belonged to domain 4 of the perlecan core protein . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
Upon VP stimulation the level of PIP ) declined , but only transiently , while increases in inositol 1 , 4 , 5 trisphosphate ( InsP 3 ) and DAG mass were sustained , suggesting that efficient resynthesis of PIP 2 allowed sustained PLC activity . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
The NE assembly process is here dissected in relation to the requirement for endogenous phosphoinositide metabolism , employing recombinant eukaryotic PI PLC , inhibitors and direct phospholipid analysis using ESI MS ( electrospray ionization mass spectrometry ) . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
Body mass did not differ before drinking ; however , before the start the subjects were heavier in the CIT trial ( 74 . 2 + / 6 . 1 kg ) vs . the PLC trial ( 73 . 4 + / 6 . 2 kg , p = 0 . 048 ) . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
PLC ( eta ) 2 consists of 1164 amino acids with a molecular mass of 125 kDa . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
Immunoblotting indicated a molecular mass with 66 . 5kDa PLC plays key role in the development of thermotolerance in pea leaves . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
RESULTS : Mass spectrometry followed by peptide mass fingerprinting conclusively identified four of the five spots as gelsolin , perlecan ( a heparan sulfate proteoglycan ) , albumin , and immunoglobulin ( P < 0 . 05 and protein scores > 67 ) . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
The increased muscle mass as well as the mRNA levels for PLC beta 1 and beta 3 in atria and RV , unlike PLC beta 3 gene expression in LV , at 3 days of AVshunt were attenuated by losartan . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
The aim of the present study was to compare salivary gland scintigraphy ( SGS ) and salivary gland ultrasonography ( SUS ) in identifying salivary gland function in patients first diagnosed with SjS . ^^^ In the remaining 3 / 20 cases of SjS both tests , SUS and SGS , showed normal results . ^^^ Discrepancies between SGS findings and labial biopsy as found in two of our cases have been reported by others and may be due among other causes to early lymphocytic infiltration in SjS . ^^^ In conclusion , in cases first diagnosed with SjS , the function of the parotid and the submandibular glands was better identified by the SGS as compared to SUS . ^^^ Although SGS is a more complex and expensive examination , it should be preferred to SUS as more sensitive and indicating the stage of SjS . . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
The WMS at 130 b / min improved significantly after PLC vs PL from 18 . 66 + / 2 . 46 to 19 . 50 + / 2 . 77 ( p less than 0 . 02 ) . ^^^ The WMS at 150 b / min improved significantly after PLC vs PL from 16 . 00 + / 2 . 95 to 16 . 91 + / 3 . 47 ( p less than 0 . 01 ) . ^^^
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
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NA
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
NA
Interacting proteins: P35555 and P98160 Pubmed SVM Score :0.0
NA