| Interacting proteins: P21781 and P98160 |
Pubmed |
SVM Score :0.58433892 |
| Thus , perlecan protein core should be considered a novel biological ligand for FGF 7 , an interaction that could influence cancer growth and tissue remodeling . . 0.58433892^^^ |
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| Interacting proteins: P21781 and P98160 |
Pubmed |
SVM Score :0.0 |
| However , perlecan core protein requirement was not related to fibroblast growth factor 7 binding because RT 101 cells were unresponsive to and lacked receptors for this growth factor . ^^^ |
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| Interacting proteins: P21781 and P98160 |
Pubmed |
SVM Score :0.0 |
| Addition of the perlecan ligand , fibroblast growth factor 7 , protected perlecan deficient keratinocytes from cell death and improved the thickness of the epidermis . ^^^ |
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| Interacting proteins: P21781 and P98160 |
Pubmed |
SVM Score :0.0 |
| Growth of colon carcinoma cells was markedly attenuated upon obliteration of perlecan gene expression and these effects correlated with reduced responsiveness to and affinity for mitogenic keratinocyte growth factor ( FGF 7 ) . ^^^ Exogenous perlecan effectively reconstituted the activity of FGF 7 in the perlecan deficient cells . ^^^ Moreover , soluble FGF 7 specifically bound immobilized perlecan in a heparan sulfate independent manner . ^^^ |
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| Interacting proteins: P21781 and P98160 |
Pubmed |
SVM Score :0.0 |
| The keratinocyte growth factor ( KGF or FGF 7 ) is unique among its family members both in its target cell specificity and its inhibition by the addition of heparin and the native heparan sulfate proteoglycan ( HSPG ) , glypican 1 in cells expressing endogenous HSPGs . ^^^ Glypican 1 abrogated the stimulatory effect of heparin , and heparin reversed the inhibitory effect of glypican 1 , indicating that this HSPG inhibits FGF 7 activities by acting , most likely , as a competitive inhibitor of stimulatory HSPG species for FGF 7 . ^^^ |
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| Interacting proteins: P21781 and P98160 |
Pubmed |
SVM Score :0.0 |
| We have previously shown that antisense targeting of the perlecan gene causes a reduced growth and responsiveness to FGF 7 [ also known as keratinocyte growth factor ( KGF ) ] in human cancer cells , and that the perlecan protein core interacts specifically with FGF 7 . ^^^ The perlecan deficient cells grew more slowly , did not respond to FGF 7 with or without the addition of heparin , and were less tumorigenic than control cells . ^^^ Paradoxically , the perlecan deficient cells displayed increased FGF 7 surface binding . ^^^ Because heparin could not substitute for perlecan , the HS chains are not critical for FGF 7 mediated signalling in this cell system . ^^^ These results provide the first genetic evidence that the perlecan protein core is a molecular entity implicated in FGF 7 binding and activation of its receptor . . ^^^ |
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| Interacting proteins: P21781 and P98160 |
Pubmed |
SVM Score :0.0 |
| Using an FGF 7 specific antibody which does not react with the FGF 7 heparan sulphate proteoglycan ( HSPG ) binding site , we showed epithelial and myoepithelial immunohistochemical staining in normal breast sections , and epithelial staining in breast carcinomas . ^^^ |
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| Interacting proteins: P21781 and P98160 |
Pubmed |
SVM Score :0.0 |
| The effects of heparan sulfate proteoglycan ( HSPG ) on binding and signaling by acidic FGF ( aFGF ) and KGF via the KGFR were studied using surface bound and soluble receptor isoforms expressed in wild type and mutant Chinese hamster ovary ( CHO ) cells lacking HSPG . ^^^ In addition to the known interaction between HSPG and KGF , we found that the KGFR also bound heparin . ^^^ The biphasic effect of heparin on KGF , but not aFGF , binding and signaling suggests that occupancy of the HSPG binding site on the KGFR may specifically inhibit KGF signaling . ^^^ |
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| Interacting proteins: P21781 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
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