| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.7664501 |
| The analysis of congenital nephropathies additionally indicates that also the HSPG side chains with their negative charges may be involved in certain diseases with filter impairment . 4 . ) Furthermore , HSPG serves as a binding site for growth factors , particularly for the basic fibroblast growth factor ( bFGF ) . 0.7664501^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.66645013 |
| Solubilized bFGF was found as a complex with the 200 kD HSPG . 0.66645013^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.97047587 |
| The interaction between FGF 2 and proteoglycans was assayed by examining the binding of 125I FGF 2 to breast cancer cell cultures as well as to cationic membranes loaded with HSPG . 0.97047587^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.93653025 |
| The balance between intracellular FGF 2 and HSPG bound secreted FGF 2 is shown not to be controlled by the availability of cell surface HSPGs , indicating that the FGF 2 secretion machinery itself is rate limiting . . 0.93653025^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Heparan sulfate proteoglycan ( HSPG ) is a major component of the muscle extracellular matrix and our previous study has shown that basic fibroblast growth factor ( bFGF ) , a member of the heparin binding growth factor ( HBGF ) family , and its receptor are present in Xenopus myotomal muscle during the period of synaptogenesis . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Basic fibroblast growth factor ( bFGF ) binds to cell surface receptor ( CSR ) proteins and to heparan sulfate proteoglycans ( HSPG ) . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| A simple procedure using human basic fibroblast growth factor ( FGF ) was utilized for the selection of COS cell mutants with defects in the biosynthesis or expression of heparan sulfate proteoglycan ( HSPG ) . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| We found that basic fibroblast growth factor ( bFGF ) binds to heparinase sensitive sites in NFTs . bFGF binding is due to a heparan sulfate proteoglycan ( HSPG ) immunocytochemically identified in NFTs . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| The modulation of gene expression of the perlecan core protein by transforming growth factor beta ( TGF beta ) and basic fibroblast growth factor ( FGF ) was examined in cultures of normal human synovial cells , and by metabolic labeling using radioactive sodium sulfate , the question of whether this specialized protein is produced by synovial cells as a proteoglycan was addressed . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| The codistribution of both types of IRs with basic fibroblast growth factor ( bFGF , FGF 2 ) in neurons and astroglia supports the notion that heparan sulfate proteoglycans ( HSPG ) in the extracellular matrix may serve as a site for storage of bFGF and assist in the bFGF induced activation of the high affinity FGF receptors linked to astroglia and neurons in these discrete areas . . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Biochemical studies have shown that basic fibroblast growth factor ( bFGF or FGF 2 ) is internalized by two pathways , after binding to either FGF tyrosine kinase receptors or to heparan sulfate proteoglycans ( HSPG ) . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Heparan sulfate proteoglycans ( HSPG ) are obligatory for receptor binding and mitogenic activity of basic fibroblast growth factor ( bFGF ) . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Heparan sulfate proteoglycan ( HSPG ) , for example , associates with several matrix components such as fibronectin , laminin , and basic fibroblast growth factor . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| The present studies examined how 125I labeled basic fibroblast growth factor ( bFGF ) bound to high affinity receptors and with lower affinity to heparan sulfate proteoglycans ( HSPG ) of cultured immature rat Leydig cells was processed . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| The double stain with PLC delta and basic fibroblast growth factor ( bFGF ) binding suggest that PLC delta is an intracellular marker , showing little overlap with bFGF binding , an extracellular marker . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Heparan sulfate proteoglycans ( HSPG ) are obligatory for receptor binding and mitogenic activity of basic fibroblast growth factor ( bFGF ) . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Cell surface expression of the basement membrane form of heparan sulfate proteoglycan ( HSPG ) , perlecan , basic fibroblast growth factor ( bFGF ) binding forms of heparan sulfate , and laminin has been examined by immunofluorescence during the attachment and outgrowth phases of mouse embryo development in vitro and in utero . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| A heparan sulfate proteoglycan ( HSPG ) from bovine cardiac plasma membrane was purified to homogeneity using either isoelectric focusing or anion exchange chromatography , followed by affinity chromatography on immobilized basic fibroblast growth factor ( bFGF ) . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| The angiogenic factor , basic fibroblast growth factor ( bFGF ) , is sequestered and protected by binding to heparan sulfate proteoglycans ( HSPG ) in the subendothelial extracellular matrix ( ECM ) . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| The degradation of human endothelial cell derived perlecan and release of bound basic fibroblast growth factor by stromelysin , collagenase , plasmin , and heparanases . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| We have investigated the interaction of basic fibroblast growth factor ( bFGF ) with its receptors and heparan sulfate proteoglycans ( HSPG ) . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| In order to obtain an insight into morphogenetic processes such as angiogenesis , cell proliferation , and tissue remodeling we have studied the localization of basic fibroblast growth factor ( bFGF ) and heparan sulfate proteoglycan ( HSPG ) in the human placenta by immunohistochemistry . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Using specific antibodies , we compared the immunolocalization of the vascular heparan sulfate proteoglycan ( HSPG ) binding protein vitronectin ( VN ) , HSPG , basic fibroblast growth factor , and collagen type 1 in retinae prepared from 11 month old diabetic and nondiabetic Wistar rats . ^^^ In normal rats , VN immunostaining was prominent in vascularized parts of the inner retina and colocalized with HSPG , which itself showed costaining with basic fibroblast growth factor in the extracellular matrix , vascular walls , and the inner limiting membrane . ^^^ In contrast , the recognition of VN , HSPG , and basic fibroblast growth factor in diabetic retinae was greatly reduced in the inner limiting membrane and the extracellular matrix , where increased immunoreactive AGE colocalizing with VN were detectable . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Angiogenic growth factors and their receptors , such as basic fibroblast growth factor ( bFGF ) and Flg , vascular endothelial growth factor ( VEGF ) and Flk 1 , platelet derived growth factor ( PDGF ) B chain and PDGF beta receptor , and five intracellular signal proteins ( phosphatidylinositol 3 kinase [ PI3K ] , phospholipase C gamma [ PLC gamma ] , C Src , SHC , and mitogen activated protein kinase [ MAPK ] ) were examined by Western blot analysis . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Furthermore the perlecan ( 1 404 ) peptide showed affinity to immobilized basic fibroblast growth factor . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Each part of the retina was prepared for Western blot analysis of tyrosine phosphorylated proteins , vascular endothelial growth factor ( VEGF ) , basic fibroblast growth factor ( bFGF ) , and four signal proteins in the tyrosine kinase pathways , which were phospholipase C gamma ( PLC gamma ) , C Src , SH 2 containing protein ( SHC ) , and mitogen activated protein kinase ( MAPK ) . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Suppression of autocrine and paracrine functions of basic fibroblast growth factor by stable expression of perlecan antisense cDNA . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| These results thus provide the first evidence that perlecan may inhibit the growth and invasiveness of fibrosarcoma cells in a basic fibroblast growth factor independent pathway and raise the possibility that perlecan may prevent the infiltration of host tissues in mesenchymal neoplasms . . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Taken together with the morphological evidence on autoradiography , it seems most likely that the injected trafermin could be inactivated in sinusoidal endothelial cells , probably through a well known internalization mechanism of the basic fibroblast growth factor HSPG complex . . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Expression of perlecan , a proteoglycan that binds myogenic inhibitory basic fibroblast growth factor , is down regulated during skeletal muscle differentiation . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Basic fibroblast growth factor ( bFGF ) is a potent mitogen for vascular smooth muscle cells ( VSMC ) and has been implicated in a number of vascular disorders . bFGF interacts with high affinity receptors and heparan sulfate proteoglycans ( HSPG ) at the cell surface . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Perlecan , a ubiquitous heparan sulfate proteoglycan , possesses angiogenic and growth promoting attributes primarily by acting as a coreceptor for basic fibroblast growth factor ( FGF 2 ) . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| The increased proliferation resulting from basic fibroblast growth factor could be attributed to perlecan , which was shown to be expressed by ensheathing cell in culture . ^^^ Perlecan is known to induce high affinity binding of basic fibroblast growth factor to receptors on cell membranes . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Both forms of perlecan bound basic fibroblast growth factor with Kd approximately 70 nM . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| The present data support the hypothesis that inhibition of endothelial cell proliferation by lead may result from a lower response of the cells to endogenous basic fibroblast growth factor whose binding to the receptor is strongly promoted by heparan sulfate chains of perlecan . . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| To support this differential regulation of perlecan mRNA by IL 1alpha , basic fibroblast growth factor ( bFGF ) , a growth factor also present in plaques , was studied in parallel . bFGF mRNA did not show any regional difference , being up regulated in both hippocampus and striatum in vivo . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Comparison of the number , localization , and phenotype of endothelial and mast cells and the distribution of basement membrane constituents ( type 4 collagen , perlecan , and laminins ) and growth factors ( basic fibroblast growth factor , vascular endothelial growth factor , transforming growth factor betas ) in the biopsy and the tissue after culture shows that many of the characteristics of the original tissues were retained in culture . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Perlecan functions as a ligand reservoir for various growth factors that become stabilized against misfolding or proteolysis and acts as a co receptor for basic fibroblast growth factor by augmenting high affinity binding and receptor activation . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Perlecan has also been postulated to promote growth factor receptor interactions , including the binding of basic fibroblast growth factor ( bFGF ) to its receptor , and to enhance mitogenesis and angiogenesis . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Basic fibroblast growth factor ( bFGF ) is a polypeptide growth factor considered to have a role in chemotaxis and mitogenesis of periodontal ligament cells ( PLC ) . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Internalization of basic fibroblast growth factor at the mouse blood brain barrier involves perlecan , a heparan sulfate proteoglycan . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| We report here that heparan sulfate proteoglycan ( HSPG ) was found in bovine aqueous humor and that it directly inhibits binding of basic fibroblast growth factor and vascular endothelial growth factor to cell surface heparan sulfate . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| One possible role for the nuclear localization of HSPG is to function as a shuttle for the nuclear transport of heparin binding growth factors , such as basic fibroblast growth factor ( FGF 2 ) . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Basic fibroblast growth factor ( FGF 2 ) activates its high affinity receptors ( FGFRs ) but also acts through interaction with heparan sulfate proteoglycans ( HSPG ) . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Down regulation of perlecan expression by cleaved and latent antithrombins was observed in both basic fibroblast growth factor ( bFGF ) stimulated and unstimulated cells , whereas the antiangiogenic antithrombins inhibited the proliferation of only bFGF stimulated HUVECs by arresting cells at the G ( 1 ) cell cycle phase . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| On the other hand , lead does not exhibit cytotoxicity but inhibits the repair of endothelial monolayers after wounding by a lower response to endogenous basic fibroblast growth factor mediated by suppression of the synthesis of perlecan , a large heparan sulfate proteoglycan . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Basic fibroblast growth factor ( bFGF ) , its receptor FGF R 1 , and coreceptor heparansulfate proteoglycan ( HSPG ) are important components involved in this angiogenic process . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Blocking the heparin binding site of cleaved or latent antithrombin by complexation with a high affinity heparin pentasaccharide abolished the serpin ' s ability to inhibit proliferation , migration , capillary like tube formation , basic fibroblast growth factor ( bFGF ) signaling , and perlecan gene expression in bFGF stimulated human umbilical vein endothelial cells . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| We have shown that extracellular signal regulated kinase was activated on cutting and loading articular cartilage , and deduced that this was due to the release of bFGF ( basic fibroblast growth factor ) from the tissue . bFGF was shown to be extracellular , and by immunohistochemistry , was present in the pericellular matrix of articular chondrocytes attached to the heparan sulphate proteoglycan perlecan . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Our results indicate that 3 8 % of the total cellular content of bFGF is associated with glycosyl Pl anchored cell surface HSPG . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Cell surface heparan sulfate proteoglycans ( HSPG ) constitute relatively low affinity binding sites for bFGF . ^^^ Furthermore , binding of bFGF to cell surface HSPG is necessary for its binding to high affinity FGF receptors and for its mitogenic activity . ^^^ In addition , bFGF is bound to HSPG in the extracellular matrix ( ECM ) . ^^^ It has been suggested that bFGF may be sequestered in ECM as part of a stable insoluble FGF HSPG complex from which it is released by specific enzymatic mechanisms when needed for mitogenic activity . . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Alz 50 , a monoclonal antibody ( MAb ) to an abnormal form of tau and bFGF binding label mutually exclusive subpopulations of neurofibrillary tangles . bFGF binding is abolished by heparinase or heparitinase treatment and therefore is most likely based on the presence of HSPG . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Both HSPG species bind bFGF . 125I bFGF bound to BCE cell cultures is readily released by either heparinase or plasmin . ^^^ We propose that this plasminogen activator mediated proteolysis provides a mechanism for the release of biologically active bFGF HSPG complexes from the extracellular matrix and that bFGF release can be regulated by the balance between factors affecting the pericellular proteolytic activity . . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| A survey of defined species of cell surface and extracellular matrix heparan sulfate proteoglycans ( HSPG ) was performed in a search for cellular proteoglycans that can promote bFGF receptor binding and biological activity . ^^^ Of the various affinity purified HSPGs tested , perlecan , the large basement membrane HSPG , is found to induce high affinity binding of bFGF both to cells deficient in HS and to soluble FGF receptors . ^^^ Heparin dependent mitogenic activity of bFGF is strongly augmented by perlecan . ^^^ In a rabbit ear model for in vivo angiogenesis , perlecan is a potent inducer of bFGF mediated neovascularization . ^^^ These results identify perlecan as a major candidate for a bFGF low affinity , accessory receptor and an angiogenic modulator . . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| The double stained preparations with anti PLC delta antibody and bFGF binding suggested that PLC delta is an intracellular marker and is not retained after neuronal death . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| We had previously demonstrated that fragments > 6 mer can inhibit the interaction between cell surface heparan sulphate proteoglycan ( HSPG ) and bFGF , and bFGF induced proliferation of adrenocortical endothelial ( ACE ) cells . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| These proteoglycans were sensitive to heparinase , demonstrating that K 562 cells synthesize bFGF binding HSPG . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Bovine coronary SMCs ( cSMCs ) express bFGF , bFGF receptor ( FGF R 1 ) , and cell membrane integrated proteoheparan sulfate ( HSPG ) . ^^^ Trypsinization generates soluble forms of the complex of bFGF with the ectodomains of the bFGF receptor and cell membrane integrated HSPG in the pericellular compartment , thus allowing quantification of pericellular bFGF by a highly specific enzyme immunoassay . ^^^ The heparin induced increase in cellular protein content includes a 60 % to 100 % increase in the expression of pericellular bFGF , FGF R 1 , and cell membrane integrated HSPG . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Heparan sulphate proteoglycan ( HSPG ) , which is composed of heparan sulphate glycosaminoglycan ( HSGAG ) , has also been shown to play an important role in the pathogenesis of tissue overgrowth by enhancing the functions of bFGF . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| HSPG may play some important roles in the regeneration of liver by interacting with various growth factors such as bFGF and HB EGF . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| The released Tat binds to HSPG through its basic region and this determines its storage into the ECM , as occurs for bFGF . . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Ligand binding studies indicated that elastase treatment decreased total binding of ( 125 ) 1 bFGF to the cell surface and affected both fibroblast growth factor receptor and heparan sulfate proteoglycan ( HSPG ) binding sites . ^^^ These findings indicate that elastase mediated HSPG release from fibroblast matrices reduces the effective affinity of bFGF for its receptor . ^^^ Collectively , these studies suggest that HSPG co receptors are important mediators of the pulmonary fibroblast response to elastase treatment and that bFGF , HSPG , and other elastase released entities play an important role in the response of the lung to chronic injury . . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| This report shows that the bFGF regulation of their expression specifically depends on the nature of HSPG and of the Sertoli cell developmental stage . ^^^ In conclusion , HSPG are partners and the target of bFGF in rat Sertoli cells . . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| The most universal angiogenic cytokines ( VEGF , bFGF , HGF ) are all heparin binding proteins , the function of which is dependent on cell surface heparan sulfate proteoglycans ( HSPG ) . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| It is noteworthy that HSPG binding protects FGF 2 from denaturation and proteolytic degradation , provides a matrix bound or cell surface reservoir of this factor for the cells and is required for the activation of FGF high affinity receptors . ^^^ Using as model system L 6 myoblasts lacking endogenous FGF receptors ( FGFRs ) , we demonstrated that these cells internalize FGF 2 efficiently through an HSPG mediated pathway . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| The HSPG exactly co localizes with FGF 2 within the neuroepithelium of the embryonic mouse . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Fibroblast growth factor 1 ( FGF 1 ) and 2 ( FGF 2 ) bind to two classes of receptors : the high affinity receptors , a family of four known transmembrane tyrosine kinases ( FGF R 1 R4 ) , and the low affinity receptors , cell surface and basement membrane heparan sulfate proteoglycan ( HSPG ) . ^^^ This change in the capacity of FGF 2 to bind to FGF R 1 and to HSPG was not due to a switch of the IIIc splice form of FGF R 1 to the IIIb splice form since the exon IIIc was the most predominant splice form of FGF R 1 during RPE cell cultures . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Our results showed that MCF 7 cells were unsensitive to FGF 2 after enzymatic degradation of heparin sulfate proteoglycans ( HSPG ) by heparinase . ^^^ The sulphated glycosaminoglycans which were critical in FGF 2 induced proliferation were strictly HSPG , as an addition of heparin in cell culture medium can restore FGF 2 mitogenic activity . ^^^ These results provide clear evidence for the critical role of HSPG in FGF 2 induced proliferation on MCF 7 breast cancer cells . . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| One such proteoglycan , HSPG PRM ( Perlecan related molecule ) , was isolated through its ability to potentiate neural cell responses to either FGF 1 or FGF 2 . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Significantly , HS sidechains from a neuroepithelial perlecan variant that preferentially bind FGF 2 , HS ( FGF 2 ) , cause aberrant targeting , whereas those that preferentially bind FGF 1 do not . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| We have examined the importance of cell surface heparan sulfate proteoglycans ( HSPG ) in fibroblast growth factor ( FGF ) signaling . 3T3 cells grown under conventional conditions were much more sensitive to FGF 2 compared to FGF 1 . ^^^ However , cells were equally sensitive to FGF 1 and FGF 2 using conditions which reduced the effect of endogenous HSPG . ^^^ Glycosaminoglycan chains or core protein fragments derived from the HSPG failed to show the same high apparent affinity for FGF 2 , suggesting that an intact proteoglycan structure was important for the high FGF 2 affinity . ^^^ Addition of HSPG ectodomains , isolated from cultured 3T3 cells or produced as recombinant molecules , to chlorate treated cultures of 3T3 cells inhibited the mitogenic activity of FGF 2 and eliminated the effect of heparin as a potentiator of either growth factor . ^^^ These results support the idea that the cell associated HSPG is an integral component of the FGF signaling system and in 3T3 cells contributes to the increased sensitivity of these cells to FGF 2 compared to FGF 1 . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Rather , a primary substrate of all receptor tyrosine kinases , phospholipase C gamma ( PLC gamma ) , is activated by both FGF 2 and Lewis 10 . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Modification of HSPG induced by TGFbeta 1 or NaB treatments in normal and breast cancer epithelial cells resulted in an increase in 125I fibroblast growth factor 2 ( FGF 2 ) binding on HSPG . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Incubation of organ sections with 2 M NaCl or heparin eluted all the radioactivity , indicating that labeling was due to FGF 2 heparan sulfate proteoglycan ( HSPG ) interactions . ^^^ All FGF 2 catabolic fragments bound heparin , demonstrating the preservation of their HSPG binding site during the in vivo intracellular catabolism of FGF 2 . ^^^ This study shows for the first time that exogenous FGF 2 is cleared by HSPG cellular internalization and catabolism without inducing the activation of FGFRs within at least five organs in vivo , which strongly suggests that the HSPG dependent internalization and catabolism pathway may control the in vivo bioavailability of FGF2 . . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Isolated heparan sulfate proteoglycan ( HSPG ) from liver cells in cell free complexes with FGFR 4 restored the specificity for FGF 1 and supported the binding of both FGF 1 and FGF 2 when complexed with FGFR 1 . ^^^ In contrast , FGF 2 bound equally well to complexes of both FGFR 1 and FGFR 4 formed with endothelial cell derived HSPG , but the endothelial HSPG was deficient for the binding of FGF 1 to both FGFR complexes . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| However , these antibodies appear to target preferentially different domains within the FGF 2 molecule , and therefore differentially influence the interactions of FGF 2 with its low and high affinity receptors . 254F1 is a more effective inhibitor of the high affinity , receptor tyrosine kinase binding site , whereas 256A12 appears to be a better inhibitor of the low affinity , HSPG interactions . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| In addition , we demonstrate this macrophage HSPG selectively binds the macrophage derived growth factors FGF 2 , vascular endothelial growth factor and heparin binding EGF and can present FGF 2 in a form that transactivates receptor bearing BaF 32 cells . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| The assay has been demonstrated using a model heparan sulfate proteoglycan preparation ( perlecan from cultured bovine endothelial cells ) and FGF 2 . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| We therefore examined FGF 2 induced effects on phosphoinositide specific phospholipase C ( PI PLC ) isozymes , which produce second messengers linked to the inotropic and hypertrophic response of the myocardium . ^^^ FGF 2 , administered by retrograde perfusion to the isolated heart , induced an increase in inositol 1 , 4 , 5 trisphosphate levels in the cytosol , as well as an increase in total PI PLC activity associated with sarcolemmal and cytosolic fractions . ^^^ Furthermore FGF 2 induced a time dependent elevation in cardiomyocyte membrane associated PLC gamma 1 and PLC beta 1 activities , assayed in immunoprecipitated fractions , and moreover , increased the membrane levels of PLC beta 1 and PLC beta 3 . ^^^ Activation of PLC beta is suggestive of FGF 2 induced cross talk between FGF receptor tyrosine kinase and G protein coupled signaling in adult cardiomyocytes and underscores the importance of FGF 2 in cardiac physiology . . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| We evaluated the expression of a select panel of growth factors and their receptors , including fibroblast growth factor 1 ( FGF 1 ) , fibroblast growth factor 2 ( FGF 2 ) , platelet derived growth factor ( PDGF ) , FGF receptor 1 ( FGF R 1 ) , FGF receptor 3 ( FGF R 3 ) , FGF receptor 4 ( FGF R 4 ) , PDGF receptor alpha ( PDGF Ralpha ) , PDGF receptor beta ( PDGF Rbeta ) , and heparan sulfate proteoglycan ( HSPG ) , in muscle biopsy specimens from nine facioscapulohumeral muscular dystrophy ( FSHD ) patients using immunohistochemistry . ^^^ |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| The interaction of fibroblast growth factor 2 ( FGF 2 ) with heparan sulfate proteoglycans ( HSPG ) has been demonstrated to enhance receptor binding and alter the intracellular distribution of internalized FGF 2 . ^^^ In the present study , the intracellular fate of FGF 2 was analyzed in vascular smooth muscle cells ( VSMC ) under native and HSPG deficient conditions . ^^^ The kinetic analysis indicated that the greatest differences between native and HSPG deficient VSMC was in the formation of LMW and further suggested that these FGF 2 products appear to represent a stable subpool of internal FGF 2 that is favored in cells that contain HSPG . ^^^ Thus , HSPG might function as a cellular switch between immediate and prolonged signal activation by heparin binding growth factors such as FGF 2 . ^^^ In the absence of HSPG , FGF 2 can interact with and activate its receptor , yet in the presence of HSPG , FGF 2 might be able to mediate prolonged or unique biological responses through intracellular processes . . ^^^ |
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| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Fibroblast growth factor 2 ( FGF 2 ) is one of the best characterized of the heparin binding growth factors and it has been shown experimentally that heparin regulation of FGF 2 activity is dependent on the level of cell HSPG and the concentration of heparin . ^^^ |
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| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| These results suggest that the HSPG receptor for FGF 2 not only influences the outcome of FGF 2 signaling , e . g . cell proliferation , but importantly regulates the immediate early signals generated by this growth factor . . ^^^ |
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| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Taken together , these results suggest that Edg 1 may be the main receptor mediating the stimulation of ERK / Egr 1 / FGF 2 system but that Edg 5 may be responsible for the stimulation of PLC Ca ( 2+ ) system and PLD in native C 6 glioma cells . . ^^^ |
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| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Here , we evaluated the effects of different heparan sulfate proteoglycan ( HSPG ) expressing cell lines and HSPGs derived from these cells on FGF 2 induced FGFR 1 phosphorylation in heparan sulfate negative BaF 3 cells . ^^^ |
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| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| In this study , we have focused on the role of FGF 2 , which requires heparan sulfate proteoglycans ( HSPG ) as cofactors for binding and activation of its cell surface tyrosine kinase receptor . ^^^ |
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| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Perlecan isolated from human umbilical arterial endothelial cells ( HUAEC ) and a continuous endothelial cell line ( C 11 STH ) bound similar amounts of FGF 2 either alone or complexed with FGFRalpha 1 IIIc or FGFR3alpha IIIc . ^^^ Interestingly , perlecan isolated from a colon carcinoma cell line that was capable of binding FGF 2 was incapable of activating any BaF 3 cell line unless the HS was removed from the protein core . ^^^ |
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| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Five micrometer sections cut from paraffin blocks were immunolabeled for collagen , heparan sulfate proteoglycans ( HSPG ) , FGF 2 , TGFbeta , and VEGF . ^^^ |
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| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Cdk 4 and p27Kip1 play a role in PLC gamma 1 mediated mitogenic signaling pathway of 18 kDa FGF 2 in corneal endothelial cells . ^^^ PURPOSE : To determine whether PLC gamma 1 enzyme activity is essential for cell proliferation in response to FGF 2 stimulation and to investigate the effect of PLC gamma 1 activation on cell division and on processes that regulate cell cycle progression . ^^^ RESULTS : When CEC were treated with FGF 2 , a prolonged and continuous FGF 2 exposure was required for both PLC enzyme activation and cell proliferation . ^^^ However , there was a long lag period between the PLC enzyme activation and cell proliferation : the maximum enzyme activity was reached 8 h after FGF 2 stimulation , but no cell proliferation was observed in the cells exposed to FGF 2 for 8 h . ^^^ Using neutralizing anti PLC gamma 1 , PLC beta 1 , or PLC delta 1 antibodies , we further demonstrated that PLC gamma 1 accounts for the hydrolysis of total phosphoinositides ( PI ) and cell proliferation mediated by FGF 2 . ^^^ |
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| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| However , it fully retains the FGF 2 binding ability of the original heparin , as shown by its capacity to protect FGF 2 from trypsin cleavage and to prevent the formation of heparan sulfate proteoglycan ( HSPG ) / FGF2 / FGFR1 ternary complexes . ^^^ However , when compared to heparin it showed a reduced capacity to induce FGF 2 dimerization and to favor the interaction of [ 125I ] FGF 2 with FGFR 1 in HSPG deficient , FGFR 1 transfected CHO cells . ^^^ |
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| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Here we show that members of three families of heparan sulfate proteoglycans , syndecan , perlecan , and glypican , are developmentally and posttranscriptionally regulated during oligodendrocyte lineage progression : Syndecan 3 is synthesized by oligodendrocyte progenitors ( but not terminally differentiated oligodendrocytes ) and is up regulated by FGF 2 ; perlecan synthesis increases as oligodendrocytes undergo terminal differentiation ; glypican 1 is expressed by both progenitors and differentiated oligodendrocytes . ^^^ |
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| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| To detect more dynamic , functional components of the epithelial mesenchymal trophic unit , we evaluated the distribution of perlecan , fibroblast growth factor 2 ( FGF 2 ) , and fibroblast growth factor receptor 1 ( FGFR 1 ) . ^^^ |
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| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| FGF 2 bound specifically to surface adsorbed fibrinogen , fibronectin and vitronectin as well as to pre coated heparan sulphate proteoglycan , perlecan . ^^^ |
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| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| To detect changes in the function of the BMZ , we measured immunoreactivity of the heparan sulfate proteoglycan , perlecan , and FGF 2 . ^^^ These areas were depleted of perlecan and FGF 2 ; however , increased FGF 2 immunoreactivity was present in the adjacent basal cells . ^^^ |
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| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Increased amounts of HSPG were present in the dermal nodules of scleromyxedema compared to adjacent normal dermis and these bound FGF 2 , immobilized the soluble receptor protein FGFR 1 and , therefore , formed a ternary complex composed of HSPG , FGF 2 and FGFR 1 in vitro . ^^^ |
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| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Satellite cells that differ in proliferation rates differ in responsiveness to FGF 2 , and in the expression of FGF 2 , FGF receptor 1 , and HSPG . . ^^^ |
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| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| To detect functional changes in the BMZ , we measured perlecan and fibroblast growth factor 2 ( FGF 2 ) . ^^^ In the absence of perlecan , FGF 2 was not bound to the BMZ . ^^^ |
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| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Moreover , using heparan sulfate proteoglycan ( HSPG ) deficient myeloid cells and porcine aortic endothelial cells ( PAECs ) expressing chimeric FGFR 1 , we show that antagonism of FGFR 1 mediated DNA synthesis and MAPK activation by SPARC does not require the presence of cell surface , low affinity FGF 2 receptors , but can be mediated by an intracellular mechanism that is independent of an interaction with the extracellular ligand binding domain of FGFR 1 . ^^^ |
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| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Interestingly , comparison of sensorgrams of sequential injections of FGF 2 and FGFR 1 and equimolar FGF 2 FGFR1 injections onto a heparin neoproteoglycan surface demonstrates that FGF 2 dramatically enhances the association of FGFR 1 with heparin and leads us to propose a model for the stepwise assembly of a ternary FGF FGFR HSPG complex . ^^^ |
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| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| CONCLUSION : FGF 2 was transferred to FGFR 1 through binding to HSPG , which is characteristically expressed on RASFs , resulting in RANKL and ICAM 1 mediated maturation of osteoclasts via ERK activation . ^^^ |
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| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| The spatiotemporal expression of syndecan 3 during feather development suggests that this cell surface HSPG may be involved in the response of competent embryonic skin dermis to FGF 2 . . ^^^ |
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| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| In contrast , initiated progenitors did not exit the cell cycle when stimulated with PDGF and / or FGF 2 . cDNA oligoarray analysis and RT PCR verification showed an early upregulation / induction of growth factor / receptors , PDGF A , PDGFR beta , IGFR 1 , IGF 1 and 2 , IL 6 , MEGF 5 , FRAG 1 , IRS 2 , HSPG , and FGFR 1 , followed by a late increase in the expression IGFBP 6 , PDGF alpha , FGFR 4A , c / ERB A , and FGFR 4 , 2 , and 1 during the tumorigenic progression . ^^^ |
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| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| In particular , heparin binding growth factors , such as fibroblast growth factor 2 ( FGF 2 ) , bind to heparan sulfate proteoglycans ( HSPG ) on the cell surface and within the extracellular matrix ( ECM ) , and these molecules have been viewed as accessory co receptors serving to facilitate tyrosine kinase receptor binding . ^^^ Recent studies , however , have indicated that HSPG can directly participate in signal transduction in response to FGF 2 binding . ^^^ We find that FGF 2 binding to its receptor correlates well with Erk1 / 2 activation and that HSPG can modulate this response through its ability to stabilize these ligand receptor complexes . ^^^ Moreover , we also observed that FGF 2 binding to HSPG correlates strongly with Erk1 / 2 activation under conditions where there is a loss of receptor activity , and we demonstrate that the relative amounts of signaling and non signaling HSPG on the cell surface , as well as the presence of competing HSPG in the ECM , can impact the signal potential via this pathway . ^^^ |
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| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| We determined that the MAPK and two PLC pathways are required for FGF 2 to stimulate RGC neurite extension in vitro , but the response of axons to FGF 2 applied asymmetrically to the growth cone depended only on the PLC pathways . . ^^^ |
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| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| We examined the intracellular dynamics of FGF 2 , FGFR 1 and the HSPGs syndecan 2 and 3 , glypican 1 and 2 , and perlecan in neurones and glia in and around adult rat cerebral wounds . ^^^ |
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| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| Glioma cell expression of the cell surface HSPG glypican 1 closely mirrored the FGF 2 coactivator activity . ^^^ |
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| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09038 and P98160 |
Pubmed |
SVM Score :0.0 |
| NA |
|