| Interacting proteins: Q8N6T3 and P84077 |
Pubmed |
SVM Score :0.0 |
| Activation of ADP ribosylation factor 1 GTPase activating protein by phosphatidylcholine derived diacylglycerols . ^^^ |
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| Interacting proteins: Q8N6T3 and P84077 |
Pubmed |
SVM Score :0.0 |
| Plasmodium yoelii : identification of a gene encoding a putative ADP ribosylation factor 1 GTPase activating protein , PyAG 1 . ^^^ |
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| Interacting proteins: Q8N6T3 and P84077 |
Pubmed |
SVM Score :0.0 |
| A complementary DNA encoding the ARF 1 GAP was cloned from rat liver and predicts a protein with a zinc finger motif near the amino terminus . ^^^ The ARF 1 GAP was localized to the Golgi complex and was redistributed into a cytosolic pattern when cells were treated with brefeldin A , a drug that prevents ARF 1 dependent association of coat proteins with the Golgi . ^^^ |
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| Interacting proteins: Q8N6T3 and P84077 |
Pubmed |
SVM Score :0.0 |
| This hypothesis was tested using Arf 1 GAP as a model system . ^^^ |
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| Interacting proteins: Q8N6T3 and P84077 |
Pubmed |
SVM Score :0.0 |
| Here , we demonstrate that the intracellular KDEL receptor , ERD 2 , self oligomerizes and interacts with ARF 1 GAP , and thereby regulates the recruitment of cytosolic ARF 1 GAP to membranes . ^^^ Because ERD 2 overexpression enhances the recruitment of GAP to membranes and results in a phenotype that reflects ARF 1 inactivation , our findings suggest that ERD 2 regulates ARF 1 GAP , and thus regulates ARF 1 mediated transport . . ^^^ |
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| Interacting proteins: Q8N6T3 and P84077 |
Pubmed |
SVM Score :0.0 |
| A zinc finger motif near the amino terminus of the ARF 1 GAP was required for stimulation of GTP hydrolysis . ^^^ |
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| Interacting proteins: Q8N6T3 and P84077 |
Pubmed |
SVM Score :0.0 |
| Using synthetic peptides , we find that the cytoplasmic domain of p24beta1 can bind Arf GTPase activating protein ( GAP ) 1 and cause direct inhibition of ArfGAP 1 mediated GTP hydrolysis on Arf 1 bound to liposomes and Golgi membranes . ^^^ |
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| Interacting proteins: Q8N6T3 and P84077 |
Pubmed |
SVM Score :0.0 |
| This degree of substrate preference was unique to DEF 1 , as other ARF GAP proteins , ACAP 1 , ACAP 2 , and ARFGAP 1 , were able to function on both ARF 1 and ARF 6 . ^^^ |
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| Interacting proteins: Q8N6T3 and P84077 |
Pubmed |
SVM Score :0.0 |
| The sorting scheme predicts a dependency on the amount of ARFGAP 1 and explains the recent experimental findings that ARF 1 and COPI detach with different time constants from the Golgi membrane in vivo . . ^^^ |
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| Interacting proteins: Q8N6T3 and P84077 |
Pubmed |
SVM Score :0.0 |
| Assembly of the coat protein 1 ( COPI ) vesicle coat is controlled by the small GTPase ADP ribosylation factor 1 ( ARF 1 ) and its GTPase activating protein , ARFGAP 1 . ^^^ Here , we investigate the diffusional behaviours of coatomer , the main component of the coat , and also those of ARF 1 and ARFGAP 1 . ^^^ Using fluorescence correlation spectroscopy , we found that most ARF 1 and ARFGAP 1 molecules are highly mobile in the cytosol ( diffusion constant D approximately equal to 15 microm ( 2 ) s ( 1 ) ) , whereas coatomer diffuses 5 10 times more slowly than expected ( D approximately equal to 1 microm ( 2 ) s ( 1 ) ) . ^^^ The addition of aluminium fluoride locks coatomer onto Golgi membranes and also decreases the binding kinetics of both ARF 1 and ARFGAP 1 , suggesting that these proteins function in concert to mediate sorting and vesicle formation . . ^^^ |
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| Interacting proteins: Q8N6T3 and P84077 |
Pubmed |
SVM Score :0.0 |
| Although COPI coat disassembly requires the catalysis of GTP hydrolysis in Arf 1 by a specific GTPase activating protein ( ArfGAP 1 ) , the precise timing of this reaction during COPI vesicle formation is not known . ^^^ Using time resolved assays for COPI dynamics on liposomes of controlled size , we show that the rate of ArfGAP 1 catalysed GTP hydrolysis in Arf 1 and the rate of COPI disassembly increase over two orders of magnitude as the curvature of the lipid bilayer increases and approaches that of a typical transport vesicle . ^^^ |
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| Interacting proteins: Q8N6T3 and P84077 |
Pubmed |
SVM Score :0.0 |
| Differences between AGAP 1 , ASAP 1 and Arf GAP 1 in substrate recognition : interaction with the N terminus of Arf 1 . ^^^ Here , we directly compare the role of amino acids 2 17 of Arf 1 , a GTP and phospholipid sensitive switch , for interaction with three Arf GAPs : Arf GAP 1 , AGAP 1 and ASAP 1 . ^^^ By analysis of point mutants , we found that lysines 15 and 16 had a greater contribution to productive interaction between Arf 1 , ASAP 1 and AGAP 1 than between Arf 1 and Arf GAP 1 . ^^^ Amino acids 2 17 of Arf 1 , isolated from the protein , inhibited GAP activity of Arf GAP 1 , ASAP 1 and AGAP 1 and bound directly to ASAP 1 . ^^^ |
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| Interacting proteins: Q8N6T3 and P84077 |
Pubmed |
SVM Score :0.0 |
| Here , we use FRAP to monitor the membrane turnover of GFP tagged versions of ArfGAP 1 , Arf 1 , and coatomer in living cells . ^^^ ArfGAP 1 underwent fast cytosol / Golgi exchange with approximately 40 % of the exchange dependent on engagement of ArfGAP 1 with coatomer and Arf 1 , and affected by secretory cargo load . ^^^ Permanent activation of Arf 1 resulted in ArfGAP 1 being trapped on the Golgi in a coatomer dependent manner . ^^^ These data suggest that ArfGAP 1 , coatomer and Arf 1 play interdependent roles in the assembly disassembly cycle of the COPI coat in vivo . . ^^^ |
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| Interacting proteins: Q8N6T3 and P84077 |
Pubmed |
SVM Score :0.0 |
| ArfGAP 1 promotes GTP hydrolysis in Arf 1 , a small G protein that interacts with lipid membranes and drives the assembly of the COPI coat in a GTP dependent manner . ^^^ |
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| Interacting proteins: Q8N6T3 and P84077 |
Pubmed |
SVM Score :0.0 |
| Arf GAP 1 ( where GAP stands for GTPase activating protein ) , which promotes GTP hydrolysis in the Arf 1 COPI complex is highly sensitive to lipid packing . ^^^ This suggests that during membrane budding , Arf 1 GTP molecules are progressively eliminated from the coated area where the membrane curvature is positive , but are protected from Arf GAP 1 at the bud neck due to the negative curvature of this region . ^^^ |
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| Interacting proteins: Q8N6T3 and P84077 |
Pubmed |
SVM Score :0.0 |
| AP 1 stimulated ArfGAP 1 activity , suggesting a role of AP 1 in the regulation of the Arf 1 `` GTPase timer . ' ' In the presence of cytosol , AP 1 could be recruited to liposomes without sorting signals , consistent with the existence of docking factors in the cytosol . ^^^ GTP and ArfGAP 1 on the membrane stimulates Arf 1 GTPase activity , whereas interaction with cargo induces oligomerization and reduces the rate of GTP hydrolysis , thus contributing to efficient cargo sorting . . ^^^ |
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| Interacting proteins: Q8N6T3 and P84077 |
Pubmed |
SVM Score :0.0 |
| In the context of biological Golgi membrane , both palmitoyl coenzyme A ( p coA ) and ARFGAP 1 , a GTPase activating protein ( GAP ) for ADP Ribosylation Factor 1 , also participate in vesicle formation , but how their roles may be linked remains unknown . ^^^ |
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| Interacting proteins: Q8N6T3 and P84077 |
Pubmed |
SVM Score :0.0 |
| The Arf 1 directed GTPase activating protein ArfGAP 1 is a Golgi localized protein that controls the dynamics of the COPI coat of carriers that mediate transport in the endoplasmic reticulum Golgi shuttle . ^^^ Mutations in the hydrophobic residues also diminished the in vitro activity of ArfGAP 1 on Arf 1 bound to Golgi membranes . ^^^ |
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| Interacting proteins: Q8N6T3 and P84077 |
Pubmed |
SVM Score :0.0 |
| To test this hypothesis , we have used mutagenesis to examine the interaction of three Arf GAPs ( ASAP 1 , AGAP 1 , and ArfGAP 1 ) with switch 1 , switch 2 , and alpha helix 3 of Arf 1 . ^^^ |
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| Interacting proteins: Q8N6T3 and P84077 |
Pubmed |
SVM Score :0.0 |
| In mice , the Szt 1 mutation , which deletes the genomic DNA encoding the KCNQ 2 C terminus and all of CHRNA 4 ( nicotinic acetylcholine receptor alpha 4 subunit ) and ARFGAP 1 ( GTPase activating protein that inactivates ADP ribosylation factor 1 ) , reduces seizure threshold , and alters M channel pharmacosensitivity . ^^^ |
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| Interacting proteins: Q8N6T3 and P84077 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8N6T3 and P84077 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8N6T3 and P84077 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8N6T3 and P84077 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8N6T3 and P84077 |
Pubmed |
SVM Score :0.0 |
| NA |
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