| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.66590994 |
| Structural elements of ADP ribosylation factor 1 required for functional interaction with cytohesin 1 . 0.66590994^^^ Cytohesin 1 and its Sec 7 domain ( C 1Sec7 ) exhibit guanine nucleotide exchange protein activity with ARF 1 but not ARF like protein 1 ( ARL 1 ) , which is 57 % identical in amino acid sequence . 0.55939808^^^ |
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| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| Cytohesin 1 also increased ARF 1 binding to a Golgi fraction , but its effect was not inhibited by brefeldin A ( BFA ) , a drug that reversibly inhibits Golgi function . ^^^ |
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| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| The PH domain of the closely related protein cytohesin 1 , which , through its Sec 7 homology domain , regulates integrin beta 2 and catalyzes guanine nucleotide exchange of the small guanine nucleotide binding protein ARF 1 , was also found to specifically bind PtdIns ( 3 , 4 , 5 ) P 3 . ^^^ |
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| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| Recently , ARNO and its close homologue cytohesin 1 were found to catalyze in vitro nucleotide exchange on ARF 1 and ARF 3 , respectively , raising the possibility that these GEFs function in the Golgi . ^^^ |
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| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| An exchange factor for human Arf 1 , ARNO , and two closely related proteins , named cytohesin 1 and GPS 1 , have been identified . ^^^ |
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| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| Solution structure of the cytohesin 1 ( B 2 1 ) Sec 7 domain and its interaction with the GTPase ADP ribosylation factor 1 . ^^^ |
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| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| Cytohesin 1 accelerated GTPgammaS ( guanosine 5 ' 3 O ( thio ) triphosphate ) binding to recombinant human ARF 1 ( rARF 1 ) , yeast ARF 3 , and ARD 1 ( a 64 kDa guanine nucleotide binding protein containing a C terminal ARF domain ) . ^^^ |
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| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| Physical interaction of the Sec 7 domain with an ARF 1 mutant was demonstrated , but it was much weaker than that of the cytohesin 1 Sec 7 domain with the same ARF protein . ^^^ |
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| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| Using a novel method to estimate GTP loading of expressed HA epitope tagged ARF proteins in intact cells , levels of biologically active , GTP bound ARF 6 as well as GTP bound ARF 1 were elevated when these ARF proteins were co expressed with GRP 1 or the related protein cytohesin 1 . ^^^ |
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| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| We show that both ARF 1 Ig and ARF 6 Ig chimeras are activated in vitro by cytohesin 1 . ^^^ In contrast , cytohesin 1 dependent GTP binding of ARF 1 is significantly enhanced by PtdInsP 3 . ^^^ |
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| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| In vitro , ARNO diglycine and cytohesin 1 triglycine enhanced phospholipase D 1 ( PLD 1 ) activation by Arf 1 with near maximal effects at 250 nM . ^^^ PLD activation was well correlated with GTP binding to Arf 1 , and cytohesin 1 was always more potent than ARNO in the PLD and GTP binding assays . ^^^ Increasing Mg2+ concentrations reduced PLD and Arf 1 activation by Arf GEFs . fMetLeuPhe and phorbol 12 myristate 13 acetate stimulated ARNO and cytohesin 1 as well as Arf 1 translocation to HL 60 cell membranes . fMetLeuPhe mediated ARNO recruitment , but not cytohesin 1 and Arf 1 translocation , was blocked by phosphatidylinositol 3 kinase inhibitors . ^^^ The combined results demonstrate that cytohesin 1 triglycine participates in a major phosphatidylinositol 3 kinase independent pathway linking cell surface receptors to Arf 1 activation and translocation in human granulocytes . . ^^^ |
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| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| In addition , overexpression of the yeast GTPase encoding genes ARF 1 and ARF 2 , but not that of YPT 1 , suppressed the sec 7 mutant growth phenotype in an allele specific manner . ^^^ |
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| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| Here we characterized a smaller human protein ( relative molecular mass 47K ) named ARNO , which contains a central Sec 7 domain that promotes guanine nucleotide exchange on ARF 1 . ^^^ |
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| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| Arno is a 47 kDa human protein recently identified as a guanine nucleotide exchange factor for ADP ribosylation factor 1 ( ARF 1 ) with a central Sec 7 domain responsible for the exchange activity and a carboxyl terminal pleckstrin homology ( PH ) domain ( Chardin , P . , Paris , S . , Antonny , B . , Robineau , S . , Braud Dufour , S . , Jackson , C . ^^^ Arno catalyzes the release of GDP more efficiently than that of GTP from [ Delta 17 ] ARF 1 , and a stable complex between Arno Sec 7 domain and nucleotide free [ Delta 17 ] ARF 1 can be isolated . ^^^ |
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| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| Further , the Sec 7 domain of GRP 1 is found to catalyze guanine nucleotide exchange of ARF 1 and 5 but not ARF 6 . ^^^ |
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| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| Here we report the crystal structure of the catalytic Sec 7 homology domain of Arno , a human GEF for ARF 1 , determined at 2 . 2 angstroms resolution . ^^^ The sites of Sec 7 domain interaction on ARF 1 have subsequently been mapped , by protein footprinting experiments , to the switch 1 and switch 2 GTPase regions , leading to a model for the interaction between ARF GTPases and Sec 7 domain exchange factors . . ^^^ |
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| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| The His 6 tagged Sec 7 domain from ySec7p ( rySec7d ) synthesized in Escherichia coli enhanced binding of guanosine 5 ' [ gamma [ 35S ] thio ] triphosphate by recombinant yeast ARF 1 ( ryARF 1 ) and ryARF 2 but not by ryARF 3 . ^^^ |
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| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| The Sec 7 domain of the guanine nucleotide exchange factor ARNO ( ARNO Sec 7 ) is responsible for the exchange activity on the small GTP binding protein ARF 1 . ^^^ ARNO Sec 7 forms a stable complex with the nucleotide free form of [ Delta 17 ] ARF 1 , a soluble truncated form of ARF 1 . ^^^ The crystal structure of ARNO Sec 7 has been solved recently , and a site directed mutagenesis approach identified a hydrophobic groove and an adjacent hydrophilic loop as the ARF 1 binding site . ^^^ We show that Glu 156 in the hydrophilic loop of ARNO Sec 7 is involved in the destabilization of Mg2+ and GDP from ARF 1 . ^^^ Moreover , [ E156K ] ARNO Sec 7 forms a complex with the Mg2+ free form of [ Delta 17 ] ARF 1 GDP without inducing the release of GDP . ^^^ |
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| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| ARNO 3 , a Sec 7 domain guanine nucleotide exchange factor for ADP ribosylation factor 1 , is involved in the control of Golgi structure and function . ^^^ We recently have identified a human protein , ARNO ( ARF nucleotide binding site opener ) , as an ARF 1 GEF that shares a conserved domain with the yeast Sec 7 protein . ^^^ Substrate specificity of ARNO like GEFs for ARF 1 depends solely on the Sec 7 domain . ^^^ |
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| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| The crystal structure of the Sec 7 domain GEF bound to nucleotide free ARF 1 GTPase has been determined at 2 . 8 A resolution and the structure of ARF 1 in the GTP analog form determined at 1 . 6 A resolution . ^^^ The Sec 7 domain binds to the switch regions of ARF 1 and inserts residues directly into the GTPase active site . ^^^ |
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| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| Dual interaction of ADP ribosylation factor 1 with Sec 7 domain and with lipid membranes during catalysis of guanine nucleotide exchange . ^^^ Sec 7 domains catalyze the replacement of GDP by GTP on the G protein ADP ribosylation factor 1 ( myrARF 1 ) by interacting with its switch 1 and 2 regions and by destabilizing , through a glutamic finger , the beta phosphate of the bound GDP . ^^^ |
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| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| Binding site of brefeldin A at the interface between the small G protein ADP ribosylation factor 1 ( ARF 1 ) and the nucleotide exchange factor Sec 7 domain . ^^^ |
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| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| The crystal structure of the ARF1 * GDP * Sec7 * BFA complex shows that BFA binds at the protein protein interface to inhibit conformational changes in ARF 1 required for Sec 7 to dislodge the GDP molecule . ^^^ |
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| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| The proteins Arno and Gea 2 of the Sec 7 family can promote GDP GTP exchange on Arf 1 , a small GTP binding protein , which coordinates coated vesicle formation for protein transport within the cell . ^^^ Crystal structures of the essential Sec 7 domain ( Sec7d ) of Gea 2 in the free and Arf 1 bound forms suggest that conformational dynamics of the Sec7d as well as those of the G protein play a role in nucleotide exchange . ^^^ Starting from a set of complementary crystal structures , we compared the collective movements of unbound Gea 2 and Arno Sec 7 domains , Arf 1 GDP , and the Arf 1 Gea2 ( Sec7d ) nucleotide free complex using normal modes analyses . ^^^ In all unbound Sec7d analyses , significant low energy movements were found to lead to closure of the hydrophobic groove towards the form seen in the Arf 1 Gea2 ( Sec7d ) complex , suggesting that groove closure is a general feature of the Sec 7 family . ^^^ |
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| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| We have identified an important functional region of the yeast Arf 1 activator Gea2p upstream of the catalytic Sec 7 domain and characterized a set of temperature sensitive ( ts ) mutants with amino acid substitutions in this region . ^^^ |
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| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| Yeast Arl1p directly interacts with Mon2p / Ysl2p , a protein that displays some sequence homology to the large Sec 7 guanine exchange factors ( GEFs ) of Arf 1 . ^^^ |
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| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| However , unlike these `` large ' ' Arf GEFs , Mon 2 lacks the Sec 7 domain that catalyzes nucleotide exchange on Arf 1 . ^^^ |
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| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| BFA inhibits the activation of Arf 1 by its GEFs in vivo by stabilizing an abortive complex between Arf GDP and the catalytic Sec 7 domain of some of its GEFs . ^^^ |
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| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15438 and P84077 |
Pubmed |
SVM Score :0.0 |
| NA |
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