| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| We have examined the relationship between the expression of hepcidin in the liver and the expression of the iron transport molecules divalent metal transporter 1 , duodenal cytochrome b , hephaestin and Ireg 1 in the duodenum of rats switched from an iron replete to an iron deficient diet or treated to induce an acute phase response . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| However , studies characterizing the function and regulation of Nramp 1 , DMT 1 , HFE , FPN 1 , CD 163 , and hepcidin are rapidly expanding our knowledge of the molecular aspects of RE iron handling . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| We found unexpected alterations in the expression of Slc39a1 ( mouse ortholog of SLC11A3 ) and Cybrd 1 , which encode key iron transport proteins , and Hamp ( hepcidin antimicrobial peptide ) , a hepatic regulator of iron transport . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| Circulating hepcidin then directly influences the expression of Ireg 1 in the mature villus enterocytes of the duodenum , thereby regulating iron absorption in response to body iron requirements . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| Levels of type 1 hereditary hemochromatosis gene ( HFE ) , transferrin , hepcidin , transferrin receptors 1 and 2 ( TfR 1 , TfR 2 ) , ferroportin 1 ( FPN 1 ) , divalent metal transporter 1 ( DMT 1 ) , natural resistance associated macrophage protein 1 ( Nramp 1 ) , ceruloplasmin , hephaestin , and glyceraldehyde 3 phosphate dehydrogenase ( GAPDH ) , were measured by quantitative reverse transriptase polyerase chain reaction ( qRT PCR ) . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| Hepcidin regulation of ferroportin 1 expression in the liver and intestine of the rat . ^^^ The possibility that hepcidin regulates the expression of ferroportin 1 ( FPT 1 ) , the basolateral iron transporter , was examined in rats after administration of LPS , an iron chelator , or His tagged recombinant hepcidin ( His rHepc ) . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| Duodenal DMT 1 and Ireg 1 protein levels , duodenal DMT 1 , Ireg 1 , Dcytb , hephaestin , and TfR 1 mRNA levels , and hepatic hepcidin mRNA levels were quantified and the correlation to liver iron contents was calculated . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| To investigate this possible association , we studied the hepatic expression of the gene for hepcidin ( HAMP ) and a gene important in iron transport ( IREG 1 ) in patients with haemochromatosis , in normal controls , and in Hfe knockout mice . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| These advances include the discoveries of the HFE 1 gene , a series of transmembrane iron transporters or cotransporters ( eg , divalent metal transporter 1 , duodenal cytochrome b , ferroportin 1 , hephaestin , and transferrin receptor 2 ) , and two key regulatory proteins named hepcidin and hemojuvelin . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| METHODS : Rats at various days of gestation and 24 48 hours post partum were examined for hepatic expression of hepcidin , transferrin receptors 1 and 2 , and HFE ( the gene mutated in the most prevalent form of hereditary haemochromatosis ) , and duodenal expression of divalent metal transporter 1 ( DMT 1 ) , duodenal cytochrome b ( Dcytb ) , iron regulated mRNA ( Ireg 1 ) , and hephaestin ( Hp ) by ribonuclease protection assay , western blotting , and immunohistochemistry . ^^^ Duodenal expression of the iron transport molecules DMT 1 , Dcytb , and Ireg 1 increased during pregnancy , and this corresponded with a reduction in hepcidin , HFE , and transferrin receptor 2 expression in the liver . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| Recently , other forms of HH that are not related to HFE , but are due to mutations in genes coding iron transport proteins ( ferroportin 1 , TfR 2 , hepcidin ) have been described . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| Iron release from macrophages after erythrophagocytosis is up regulated by ferroportin 1 overexpression and down regulated by hepcidin . ^^^ Treatment of cells with the peptide hormone hepcidin , a systemic regulator of iron metabolism , dramatically decreased FPN 1 protein levels and significantly reduced the efflux of 59Fe after erythrophagocytosis . ^^^ Subsequent fractionation of the total released 59Fe into heme and nonheme compounds revealed that hepcidin treatment reduced the release of nonheme 59Fe by 50 % and 25 % from control and FPN 1 overexpressing cells , respectively , but did not diminish efflux of 59Fe heme . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| Four genes are responsible for the distinct types of non HFE haemochromatosis : hepcidin and hemojuvelin are the genes involved in type 2 or juvenile haemochromatosis , transferrin receptor 2 is involved in type 3 haemochromatosis , and ferroportin 1 is mutated in type 4 , the atypical dominant form of primary iron overload . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| The latter involves regulation of the iron transporter ferroportin 1 by hepcidin . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| Quantitative real time RT PCR revealed a significant decrease in mean hepatic transcript levels of the secreted iron regulator hepcidin and increased intestinal expression of fpn 1 in anemic weh ( Tp85c / ) adults . ^^^ Injection of iron dextran into WT or mutant zebrafish embryos , however , resulted in significant increases in hepcidin expression 18 hours after injection , demonstrating that hepcidin expression in zebrafish is iron responsive and independent of fpn 1 ' s function as an iron exporter . . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| The molecular details of the connection between iron metabolism , hepcidin and inflammation have become clearer with the recent finding of hepcidin induced internalization and degradation of FPN 1 . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| Expression of ferroportin 1 protein on the cell surface is regulated by the interaction of ferroportin 1 with hepcidin . ^^^ Hepcidin treatment of cells results in internalization and lysosomal degradation of cell surface ferroportin 1 . ^^^ The clinical phenotypes observed in patients may be secondary to cell type specific defects in hepcidin mediated inhibition of ferroportin 1 expression . . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| The genes coding for HFE , transferrin receptor 2 ( TFR 2 ) , ferroportin ( SLC40A1 or FPN 1 ) , hepcidin ( HEPC ) and hemojuvelin ( HJV or RGMC ) are responsible for different types of genetic iron overload . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| Delayed upregulation of the negative hormonal regulator of iron homeostasis , hepcidin ( Hamp ) , during postnatal development correlates strongly with profound increases in Fpn 1 protein levels and polycythemia in Pcm heterozygotes . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| In contrast , iron efflux and iron regulated gene 1 ( IREG 1 ) expression were unaffected by hepcidin . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| Ferroportin is encoded by the SLC40A1 gene and mediates iron export from cells by interacting with hepcidin . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| A significant delay in developmental up regulation of hepcidin ( Hamp ) , the pivotal hormonal regulator of iron homeostasis , correlated with high levels of Fpn 1 expression in hepatic Kupffer cells and duodenal epithelial cells at 7 weeks of age . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| Hepcidin is a negative regulator of iron absorption produced mainly by the liver in response to changes in iron stores and inflammation , and its levels have been shown to regulate the intestinal basolateral iron transporter ferroportin 1 ( Fp 1 ) . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| At mid ( PN 10 ) and late ( PN 20 ) infancy , tissue Fe distribution , Fe absorption , intestine DMT 1 , ferroportin 1 ( FPN ) and hephaestin expression , and localization and liver hepcidin expression were measured . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| Liver hepcidin expression was significantly downregulated by copper deficiency ( approximately 60 % of controls ) , and enterocyte mRNA and protein levels of ferroportin 1 were increased to 2 . 5 and 10 times , respectively , relative to controls , by copper deficiency , indicating a systemic iron deficiency in the copper deficient mice . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| Coding regions and splice sites in genes encoding hepcidin and haemojuvelin were sequenced and previously described mutations in ferroportin 1 and transferrin receptor 2 genes were screened . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| Consistent with profound defects in iron homeostasis , Hif1alpha / yolk sac and / or embryos demonstrated aberrant mRNA levels of hepcidin , Fpn 1 , Irp 1 , and frascati . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Ferroportin 1 , transferrin receptor 2 and HAMP mRNA levels displayed no significant strain differences . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| The activity of ferroportin 1 is controlled by the liver derived peptide hepcidin , and the expression of hepcidin in turn is influenced by plasma transferrin saturation via a pathway that involves HFE , TfR 2 , and hemojuvelin . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| The results showed : ( 1 ) the body iron status in MG was kept at a high level compared to that of CG and SG , ( 2 ) Western blot showed DMT 1 with iron responsive element ( IRE ) and FPN 1 in duodenal epithelium which were higher in MG than that of CG and ( 3 ) the expression of hepatic hepcidin mRNA was down regulated in MG ( p < 0 . 05 ) . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| The possible involvement of genes affecting iron homeostasis , including HFE , SLC40A1 , HAMP and CYBRD 1 , was investigated in individuals who were referred for confirmation or exclusion of a diagnosis of haemochromatosis , but who tested negative or were heterozygous for the causative HFE mutation , C282Y . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| Genetic hemochromatosis now corresponds to six diseases , namely classical hemochromatosis HFE 1 ; juvenile hemochromatosis HFE 2 due to mutations in an unidentified gene on chromosome 1 ; hemochromatosis HFE 3 due to mutations in the transferrin receptor 2 ( TfR 2 ) ; hemochromatosis HFE 4 caused by a mutation in the H subunit of ferritin ; and hemochromatosis HFE 6 whose gene is hepcidine ( HAMP ) . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| Four types have been identified : type 1 is the common form and is an autosomal recessive disorder of low penetrance strongly associated with mutations in the HFE gene on chromosome 6 ( p21 . 3 ) ; type 2 ( juvenile haemochromatosis ) is autosomal recessive , of high penetrance with causative mutations identified in the HFE 2 gene on chromosome 1 ( q 21 ) and the HAMP gene on chromosome 19 ( q 13 ) ; type 3 is also autosomal recessive with mutations in the TfR 2 gene on chromosome 3 ( 7q22 ) ; type 4 is an autosomal dominant condition with heterozygous mutations in the ferroportin 1 gene . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| No coding region mutation of HFE , FPN 1 , TFR 2 , HAMP , or HJV genes was detected . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| Tumor necrosis factor alpha regulates the mRNA levels of HAMP , IREG 1 , DMT 1 and TfR 2 in cultured hepatocytes from both iron loaded and control animals . . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| The patient did not have coding region mutations in HAMP , FPN 1 , HJV or ALAS 2 . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| Four types of inherited iron overload have been recognized : type 1 , the most common form with an autosomal recessive inheritance , is associated with mutations in the HFE gene on chromosome 6 ; type 2 ( juvenile hemochromatosis ) is an autosomal recessive disorder with causative mutations identified in the HJV gene ( subtype A ) on chromosome 1 and the HAMP gene ( subtype B ) on chromosome 19 ; type 3 has also an autosomal recessive inheritance with mutations in the TfR 2 gene on chromosome 3 ; type 4 is an autosomal dominant condition with heterozygous mutations in the ferroportin 1 gene on chromosome 2 . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| Sequencing of TFR 2 , HFE , FPN 1 ( SLC40A1 ) , HAMP , HJV , and the erythrocyte pyruvate kinase genes of family members was also performed . ^^^ |
|
| Interacting proteins: P81172 and Q9NP59 |
Pubmed |
SVM Score :0.0 |
| However , iron had no effect on HAMP expression but was able to upregulate both DMT 1 and FPN 1 in alveolar macrophages . ^^^ In fact , the LPS induced alterations in the expression of HAMP as well as DMT 1 and FPN 1 were preserved in the alveolar macrophages isolated from IL 1 receptor or IL 6 deficient mice . ^^^ In addition , treatment of these cells with either LPS or HAMP caused the diminishment of the surface FPN 1 . ^^^ Our studies suggest that iron mobilization by alveolar macrophages can be affected by iron and LPS via several pathways , including HAMP mediated degradation of FPN 1 , and that these cells may use unique regulatory mechanisms to cope with iron imbalance in the lung . . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| Levels of type 1 hereditary hemochromatosis gene ( HFE ) , transferrin , hepcidin , transferrin receptors 1 and 2 ( TfR 1 , TfR 2 ) , ferroportin 1 ( FPN 1 ) , divalent metal transporter 1 ( DMT 1 ) , natural resistance associated macrophage protein 1 ( Nramp 1 ) , ceruloplasmin , hephaestin , and glyceraldehyde 3 phosphate dehydrogenase ( GAPDH ) , were measured by quantitative reverse transriptase polyerase chain reaction ( qRT PCR ) . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| Hepcidin regulation of ferroportin 1 expression in the liver and intestine of the rat . ^^^ The possibility that hepcidin regulates the expression of ferroportin 1 ( FPT 1 ) , the basolateral iron transporter , was examined in rats after administration of LPS , an iron chelator , or His tagged recombinant hepcidin ( His rHepc ) . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| These advances include the discoveries of the HFE 1 gene , a series of transmembrane iron transporters or cotransporters ( eg , divalent metal transporter 1 , duodenal cytochrome b , ferroportin 1 , hephaestin , and transferrin receptor 2 ) , and two key regulatory proteins named hepcidin and hemojuvelin . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Ferroportin 1 , transferrin receptor 2 and HAMP mRNA levels displayed no significant strain differences . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| Recently , other forms of HH that are not related to HFE , but are due to mutations in genes coding iron transport proteins ( ferroportin 1 , TfR 2 , hepcidin ) have been described . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| Four types have been identified : type 1 is the common form and is an autosomal recessive disorder of low penetrance strongly associated with mutations in the HFE gene on chromosome 6 ( p21 . 3 ) ; type 2 ( juvenile haemochromatosis ) is autosomal recessive , of high penetrance with causative mutations identified in the HFE 2 gene on chromosome 1 ( q 21 ) and the HAMP gene on chromosome 19 ( q 13 ) ; type 3 is also autosomal recessive with mutations in the TfR 2 gene on chromosome 3 ( 7q22 ) ; type 4 is an autosomal dominant condition with heterozygous mutations in the ferroportin 1 gene . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| Iron release from macrophages after erythrophagocytosis is up regulated by ferroportin 1 overexpression and down regulated by hepcidin . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| Four genes are responsible for the distinct types of non HFE haemochromatosis : hepcidin and hemojuvelin are the genes involved in type 2 or juvenile haemochromatosis , transferrin receptor 2 is involved in type 3 haemochromatosis , and ferroportin 1 is mutated in type 4 , the atypical dominant form of primary iron overload . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| The latter involves regulation of the iron transporter ferroportin 1 by hepcidin . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| Expression of ferroportin 1 protein on the cell surface is regulated by the interaction of ferroportin 1 with hepcidin . ^^^ Hepcidin treatment of cells results in internalization and lysosomal degradation of cell surface ferroportin 1 . ^^^ The clinical phenotypes observed in patients may be secondary to cell type specific defects in hepcidin mediated inhibition of ferroportin 1 expression . . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| The activity of ferroportin 1 is controlled by the liver derived peptide hepcidin , and the expression of hepcidin in turn is influenced by plasma transferrin saturation via a pathway that involves HFE , TfR 2 , and hemojuvelin . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| Four types of inherited iron overload have been recognized : type 1 , the most common form with an autosomal recessive inheritance , is associated with mutations in the HFE gene on chromosome 6 ; type 2 ( juvenile hemochromatosis ) is an autosomal recessive disorder with causative mutations identified in the HJV gene ( subtype A ) on chromosome 1 and the HAMP gene ( subtype B ) on chromosome 19 ; type 3 has also an autosomal recessive inheritance with mutations in the TfR 2 gene on chromosome 3 ; type 4 is an autosomal dominant condition with heterozygous mutations in the ferroportin 1 gene on chromosome 2 . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| Coding regions and splice sites in genes encoding hepcidin and haemojuvelin were sequenced and previously described mutations in ferroportin 1 and transferrin receptor 2 genes were screened . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| We have examined the relationship between the expression of hepcidin in the liver and the expression of the iron transport molecules divalent metal transporter 1 , duodenal cytochrome b , hephaestin and Ireg 1 in the duodenum of rats switched from an iron replete to an iron deficient diet or treated to induce an acute phase response . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| Circulating hepcidin then directly influences the expression of Ireg 1 in the mature villus enterocytes of the duodenum , thereby regulating iron absorption in response to body iron requirements . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| Duodenal DMT 1 and Ireg 1 protein levels , duodenal DMT 1 , Ireg 1 , Dcytb , hephaestin , and TfR 1 mRNA levels , and hepatic hepcidin mRNA levels were quantified and the correlation to liver iron contents was calculated . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| To investigate this possible association , we studied the hepatic expression of the gene for hepcidin ( HAMP ) and a gene important in iron transport ( IREG 1 ) in patients with haemochromatosis , in normal controls , and in Hfe knockout mice . ^^^ HAMP and IREG 1 mRNA concentrations were examined by ribonuclease protection assays and expressed relative to the housekeeping gene GAPD . ^^^ There was a significant correlation between hepatic iron concentration and expression of HAMP ( r=0 . 59 , p=0 . 02 ) and IREG 1 ( r=0 . 67 , p=0 . 007 ) in untreated patients . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| METHODS : Rats at various days of gestation and 24 48 hours post partum were examined for hepatic expression of hepcidin , transferrin receptors 1 and 2 , and HFE ( the gene mutated in the most prevalent form of hereditary haemochromatosis ) , and duodenal expression of divalent metal transporter 1 ( DMT 1 ) , duodenal cytochrome b ( Dcytb ) , iron regulated mRNA ( Ireg 1 ) , and hephaestin ( Hp ) by ribonuclease protection assay , western blotting , and immunohistochemistry . ^^^ Duodenal expression of the iron transport molecules DMT 1 , Dcytb , and Ireg 1 increased during pregnancy , and this corresponded with a reduction in hepcidin , HFE , and transferrin receptor 2 expression in the liver . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| Hepcidin is a negative regulator of iron absorption produced mainly by the liver in response to changes in iron stores and inflammation , and its levels have been shown to regulate the intestinal basolateral iron transporter ferroportin 1 ( Fp 1 ) . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| At mid ( PN 10 ) and late ( PN 20 ) infancy , tissue Fe distribution , Fe absorption , intestine DMT 1 , ferroportin 1 ( FPN ) and hephaestin expression , and localization and liver hepcidin expression were measured . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| Liver hepcidin expression was significantly downregulated by copper deficiency ( approximately 60 % of controls ) , and enterocyte mRNA and protein levels of ferroportin 1 were increased to 2 . 5 and 10 times , respectively , relative to controls , by copper deficiency , indicating a systemic iron deficiency in the copper deficient mice . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| Quantitative real time RT PCR revealed a significant decrease in mean hepatic transcript levels of the secreted iron regulator hepcidin and increased intestinal expression of fpn 1 in anemic weh ( Tp85c / ) adults . ^^^ Injection of iron dextran into WT or mutant zebrafish embryos , however , resulted in significant increases in hepcidin expression 18 hours after injection , demonstrating that hepcidin expression in zebrafish is iron responsive and independent of fpn 1 ' s function as an iron exporter . . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| The molecular details of the connection between iron metabolism , hepcidin and inflammation have become clearer with the recent finding of hepcidin induced internalization and degradation of FPN 1 . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| The genes coding for HFE , transferrin receptor 2 ( TFR 2 ) , ferroportin ( SLC40A1 or FPN 1 ) , hepcidin ( HEPC ) and hemojuvelin ( HJV or RGMC ) are responsible for different types of genetic iron overload . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| The results showed : ( 1 ) the body iron status in MG was kept at a high level compared to that of CG and SG , ( 2 ) Western blot showed DMT 1 with iron responsive element ( IRE ) and FPN 1 in duodenal epithelium which were higher in MG than that of CG and ( 3 ) the expression of hepatic hepcidin mRNA was down regulated in MG ( p < 0 . 05 ) . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| A significant delay in developmental up regulation of hepcidin ( Hamp ) , the pivotal hormonal regulator of iron homeostasis , correlated with high levels of Fpn 1 expression in hepatic Kupffer cells and duodenal epithelial cells at 7 weeks of age . ^^^ Conversely , upon up regulation of Hamp expression at 12 weeks of age , Fpn 1 expression decreased , indicative of a Hamp mediated homeostatic loop . ^^^ Thus , similar to the anemia of chronic disease , these findings demonstrate decreased iron bioavailability due to sustained down regulation of Fpn 1 levels by Hamp . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| The patient did not have coding region mutations in HAMP , FPN 1 , HJV or ALAS 2 . ^^^ |
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| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| In contrast , iron efflux and iron regulated gene 1 ( IREG 1 ) expression were unaffected by hepcidin . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| Tumor necrosis factor alpha regulates the mRNA levels of HAMP , IREG 1 , DMT 1 and TfR 2 in cultured hepatocytes from both iron loaded and control animals . . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| However , studies characterizing the function and regulation of Nramp 1 , DMT 1 , HFE , FPN 1 , CD 163 , and hepcidin are rapidly expanding our knowledge of the molecular aspects of RE iron handling . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| Delayed upregulation of the negative hormonal regulator of iron homeostasis , hepcidin ( Hamp ) , during postnatal development correlates strongly with profound increases in Fpn 1 protein levels and polycythemia in Pcm heterozygotes . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| No coding region mutation of HFE , FPN 1 , TFR 2 , HAMP , or HJV genes was detected . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| The possible involvement of genes affecting iron homeostasis , including HFE , SLC40A1 , HAMP and CYBRD 1 , was investigated in individuals who were referred for confirmation or exclusion of a diagnosis of haemochromatosis , but who tested negative or were heterozygous for the causative HFE mutation , C282Y . ^^^ |
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| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| Ferroportin is encoded by the SLC40A1 gene and mediates iron export from cells by interacting with hepcidin . ^^^ |
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| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| Sequencing of TFR 2 , HFE , FPN 1 ( SLC40A1 ) , HAMP , HJV , and the erythrocyte pyruvate kinase genes of family members was also performed . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| However , iron had no effect on HAMP expression but was able to upregulate both DMT 1 and FPN 1 in alveolar macrophages . ^^^ In fact , the LPS induced alterations in the expression of HAMP as well as DMT 1 and FPN 1 were preserved in the alveolar macrophages isolated from IL 1 receptor or IL 6 deficient mice . ^^^ In addition , treatment of these cells with either LPS or HAMP caused the diminishment of the surface FPN 1 . ^^^ Our studies suggest that iron mobilization by alveolar macrophages can be affected by iron and LPS via several pathways , including HAMP mediated degradation of FPN 1 , and that these cells may use unique regulatory mechanisms to cope with iron imbalance in the lung . . ^^^ |
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| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| Consistent with profound defects in iron homeostasis , Hif1alpha / yolk sac and / or embryos demonstrated aberrant mRNA levels of hepcidin , Fpn 1 , Irp 1 , and frascati . ^^^ |
|
| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| Genetic hemochromatosis now corresponds to six diseases , namely classical hemochromatosis HFE 1 ; juvenile hemochromatosis HFE 2 due to mutations in an unidentified gene on chromosome 1 ; hemochromatosis HFE 3 due to mutations in the transferrin receptor 2 ( TfR 2 ) ; hemochromatosis HFE 4 caused by a mutation in the H subunit of ferritin ; and hemochromatosis HFE 6 whose gene is hepcidine ( HAMP ) . ^^^ |
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| Interacting proteins: Q9NP59 and P81172 |
Pubmed |
SVM Score :0.0 |
| We found unexpected alterations in the expression of Slc39a1 ( mouse ortholog of SLC11A3 ) and Cybrd 1 , which encode key iron transport proteins , and Hamp ( hepcidin antimicrobial peptide ) , a hepatic regulator of iron transport . ^^^ |
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