| Interacting proteins: P78545 and P19838 |
Pubmed |
SVM Score :0.0 |
| Comparison of the immediate promoter regions of the human and mouse ELF 3 genes demonstrates the presence of TATA and CCAAT boxes as well as potential binding sites for Ets factors and NF kappaB . ^^^ |
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| Interacting proteins: P78545 and P19838 |
Pubmed |
SVM Score :0.0 |
| Taken together , our findings demonstrate that co ordinate activation and binding of ESE 1 , Sp 1 , and NF kappaB to the MIP 3alpha promoter is required for maximal gene expression by cytokine stimulated Caco 2 human intestinal epithelial cells . . ^^^ |
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| Interacting proteins: P78545 and P19838 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : To investigate the expression of the novel Ets transcription factor ESE 1 in rheumatoid synovium and in cells derived from joint tissues , and to analyze the role of nuclear factor kappaB ( NF kappaB ) as one of the central downstream targets in mediating the induction of ESE 1 by proinflammatory cytokines . ^^^ The wild type ESE 1 promoter and the ESE 1 promoter mutated in the NF kappaB site were cloned into a luciferase reporter vector and analyzed in transient transfections . ^^^ Electrophoretic mobility shift assays ( EMSAs ) and supershift assays with antibodies against members of the NF kappaB family were conducted using the NF kappaB site from the ESE 1 promoter as a probe . ^^^ Transient transfection experiments and EMSAs showed that induction of ESE 1 gene expression by IL 1beta requires activation of NF kappaB and binding of p 50 and p 65 family members to the NF kappaB site in the ESE 1 promoter . ^^^ Chromatin immunoprecipitation further confirmed that NF kappaB binds to the ESE 1 promoter in vivo . ^^^ |
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