Pubmed abstracts for Protein-Protein Interaction search result :


Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
DNA PKcs is a member of the phosphatidylinositol 3 kinase superfamily , which includes the human protein ATM ( ataxia telangiectasia mutated ) and the yeast protein Tel 1 . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
Our observation clearly indicates that DNA PK catalytic subunit does not phosphorylate p 53 Ser 18 in vivo or control the transactivation of p 53 in response to DNA damage , and these results further emphasize the different pathways in which ataxia telangiectasia mutated ( ATM ) and DNA PK operate following radiation damage . . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
These results indicated p 60 as a phosphorylated form of XRCC 4 , requiring DNA PKcs but not ataxia telangiectasia mutated ( ATM ) . . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
Ataxia telangiectasia mutated ( ATM ) , ataxia telangiectasia and Rad 3 related ( ATR ) and DNA dependent protein kinase catalytic subunit ( DNA PKcs ) are members of the phosphoinositide 3 kinase related protein kinase ( PIKK ) family , and are rapidly activated in response to DNA damage . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
The dependency of this phosphorylation on the various PI3K related protein kinases ( in mammals , ataxia telangiectasia mutated and Rad 3 related [ ATR ] , ataxia telangiectasia mutated [ ATM ] , and DNA PKCs ) has been a subject of debate ; it has been suggested that ATM is required for the induction of foci at DSBs , whereas ATR is involved in the recognition of stalled replication forks . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
We investigated the role in meiosis of the three kinases [ ATM ( ataxia telangiectasia mutated ) , ATR ( ataxia telangiectasia and Rad 3 related ) and DNA PKcs ( DNA dependent protein kinase catalytic subunit ) ] known to phosphorylate H2AX in mitotic cells . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
Independent apoptosis disrupts early organogenesis in embryos lacking both ataxia telangiectasia mutated and Prkdc . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
First , independently from NuA 4 , Tip 60 binds the kinases ataxia telangiectasia mutated ( ATM ) and DNA dependent protein kinase catalytic subunit ( DNA PKcs ) and participates in their activation by DNA double strand breaks . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
To clarify the involvement of DNA PKcs in this process , we analyzed DNA PKcs phosphorylation in response to UV irradiation , which causes replication stress and activates ATR ( ATM Rad 3 related ) / ATM ( ataxia telangiectasia mutated ) kinases in a replication dependent manner . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
Thus 10 ray sensitive hamster xrs 6 cells are mutated in Ku , and rodent V 3 cells and cells of the severe combined immune deficient ( Scid ) mouse lack a functional DNA PKcs . ^^^ Furthermore , the recent cloning of the gene mutated in ataxia telangiectasia ( A T ) patients , named ATM ( A T mutated ) , has revealed that the product of this gene is also a PI 3 kinase family member and is related to DNA PKcs . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
At higher concentrations , wortmannin inhibits the ataxia telangiectasia gene ( ATM ) related DNA dependent protein kinase ( DNA PKcs ) . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
DNA PK has several characteristics that support this hypothesis : Ku binds to DNA damaged by nicks or double strand breaks , DNA PKcs is activated when Ku binds to DNA , DNA PK will phosphorylate p 53 and other cell cycle regulatory proteins in vitro , and DNA PKcs shares homology with ATM , which is mutated in ataxia telangiectasia and involved in signaling the p 53 response to ionizing radiation . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
These include the ATM gene product , DNA PKcs , and the target of rapamycin ( TOR in yeast ; and FRAP in mammalian cells ) . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
Significantly , DNA PKcs null mice demonstrate complete penetrance of thymic lymphoblastic lymphomas , strongly suggesting that Prkdc functions in mice as a T cell tumour suppressor and , by virtue of its association with DNA repair and recombination , belongs to the ' caretaker ' class of tumour suppressor genes that includes ATM , BRCA 1 and BRCA 2 ( ref . 15 ) . . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
Apoptosis was detected after high dose ionizing radiation exposure , and this process was accompanied by specific degradation of DNA PKcs , ATM , and poly ( ADP ribose ) polymerase . ^^^ The radiosensitivity observed in 180BR cells can be accounted for , at least in part , by radiation induced apoptosis , and the defect in these cells is not a gross one in DNA PKcs or ATM . . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
ATM , the product of the gene mutated in ataxia telangiectasia , and DNA PKcs function in pathways that detect or repair DNA damage and are members of a family of large , serine / threonine kinases that are closely related to phosphatidylinositol 3 kinases . . ^^^ The arrangement of the PRKDC / MCM4 gene pair is similar to that of the ATM / E14 ( NPAT ) gene pair . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
Mouse cdc 21 only 0 . 5 kb upstream from dna pkcs in a head to head organization : an implication of co evolution of ATM family members and cell cycle regulating genes . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
Our results demonstrate that DNA PKcs , unlike the related ATM protein , is not essential for the activation of p 53 and G 1 cell cycle arrest in response to ionizing radiation . . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
These results provide a biochemical assay system for ATM , support genetic data indicating distinct roles for DNA dependent protein kinase and ATM , and suggest how ATM may signal the presence of DNA damage to p 53 and other downstream effectors . . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
ATM and ATR require Mn ( 2+ ) , but not DNA ends or Ku proteins , for optimal in vitro activity while DNA PKCs requires Mg ( 2+ ) , DNA ends , and Ku proteins . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
Neither M059J nor M059K cells have been fully characterized , beyond the lack of expression of PRKDC and low expression of ATM in M059J cells . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
Taken together , our results support a model in which the upstream effectors DNA PKcs and Atm selectively activate p 53 to differentially regulate cell cycle and apoptotic responses . ^^^ Whereas Atm selects for cell cycle arrest but not apoptosis , DNA PKcs selects for apoptosis but not cell cycle arrest . . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
Using Western blots we measured , in non irradiated cells , the basal expression levels of ATM , Rad 1 and Hus 1 , involved in the control of cellular DNA damage checkpoints , together with DNA PKcs , Ku 70 , Ku 80 ; XRCC 4 , ligaseIV and Rad 51 , involved in radiation induced DSB repair . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
Prkdc ( scid / scid ) mice have a homozygous mutation in the gene encoding DNA PK ( cs ) and , like Atm ( / ) mice , are viable and radiosensitive [ 4 8 ] . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
An alternative ' signal ' model of chromatid breaks is outlined whereby a single dsb , occurring within a large looped chromatin domain , is signalled ( possibly by molecules such as DNAPK or ATM protein ) and triggers the cell to undergo a recombinational exchange , either within a chromatid or between sister chromatids . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
ATM and DNA dependent protein kinase catalytic subunit ( DNA PKcs ) have been shown to have sequences homologous to the catalytic domains of mammalian phosphatidylinositol 3 kinase ( PI 3 kinase ) . ^^^ In order to determine the contribution of ATM and DNA PKcs to the increased sensitivity of cells to DNA damaging agents observed in the presence of PI 3 kinase inhibitors , we examined the effects of a PI 3 kinase inhibitor , wortmannin , on cellular sensitivity to bleomycin ( BLM ) , mitomycin C ( MMC ) , 10 irradiation and ultraviolet ( UV ) irradiation using 2 human tumor cell lines ( T98G and A 172 ) , a human fibroblast cell line ( LM 217 ) , an ataxia telangiectasia ( AT ) cell line ( AT3BISV ) , a scid murine cell line ( SCF ) and a control murine cell line ( CBF ) . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
Further , to elucidate the downstream effector pathway , we studied the 10 ray induced adaptive response in cultured mouse and human cells with different genetic background relevant to the DNA damage response pathway , such as deficiencies in TP 53 , DNA PKcs , ATM and FANCA genes . ^^^ The results showed that p 53 protein played a key role in the adaptive response while DNA PKcs , ATM and FANCA were not responsible . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
CONCLUSION : The results indicated that PKC , ATM , DNAPK and / or PI3K were involved in RAR for growth and BrdU incorporation of cultured glial cells and RAR decreased with ageing . . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
The double strand break signaling / repair proteins ATM , ATR , and DNA dependent protein kinase catalytic subunit ( DNA PK ( cs ) ) are attractive targets to confer enhanced radio and chemosensitivity to tumor cells . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
The expression of PI3K family members ATM , ATR , and DNA PKcs , and downstream genes p 53 , GADD 45 , and p 21 , was examined in the mid organogenesis rat conceptus in vivo on gestational days ( GD ) 10 through 12 and in vitro following exposure to genotoxic stress . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
Several mouse models including mice deficient in Ku , DNA PKcs ( Prkdc ) , Parp and Atm , all of which are radiosensitive in vivo , show clear telomere alterations . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
PURPOSE : To further investigate the role of DNA dependent protein kinase ( DNA PK ) and ataxia telangiectasia mutation ( ATM ) in the formation of chromosome aberrations , we analysed radiation induced aberrations in M059J cells , complemented with the PRKDC ( DNA PK ) gene by introducing a fragment of human chromosome 8 containing a copy of the human PRKDC gene . ^^^ CONCLUSIONS : Since in M059J Fus 1 cells the radioresistant phenotype with respect to chromosome type aberration formation was restored by the complementation of PRKDC , ATM expression determines the chromosomal radiosensitivity of M059J cells only to a minor extent . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
The magnitude of the DNA damage response was monitored with respect to DNA PK phosphorylation of a p 53 peptide , defence protein levels ( Ku , DNA PK catalytic subunit , ATM , p 21 and p 53 ) and flow cytometric determination of cell cycle phases and apoptosis . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
Using this assay M059J cells , which fail to express the catalytic subunit of DNA dependent protein kinase ( PRKDC , also known as DNA PKcs ) and are hypersensitive to ionizing radiation , were found to express two different aberrant ATM transcripts : one characterized by 4776 del 133 , which corresponds to the deletion of exon 33 , and the other by 4909 ins 116 . ^^^ Thus the radiosensitive phenotype of M059J cells appears to be due to a defect in PRKDC and a truncating ATM mutation . . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
Thus , Artemis is an effector of DNA repair that can be phosphorylated by ATM , and possibly by DNA PKcs and ATR depending upon the type of DNA damage . . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
Comparison of DNA PKcs with another PIKK DNA repair factor , ATM , defines a common architecture for this important protein family . . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
To further elucidate potential Artemis independent functions of DNA PKcs , we asked whether the embryonic lethality associated with double deficiency for DNA PKcs and the related ataxia telangiectasia mutated ( ATM ) kinase was also observed in mice doubly deficient for ATM and Artemis . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
DNA damage sensors ATM , ATR , DNA PKcs , and PARP 1 are dispensable for human immunodeficiency virus type 1 integration . ^^^ We conclude that the DNA repair enzymes ATM , ATR , DNA PKcs , and PARP 1 are not essential for HIV 1 integration . . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
Since deficiency of either DNA Pkcs or ATM leads to defective repair of IR induced DSB , our finding places Artemis at the signaling crossroads downstream of DNA PKcs and ATM in IR induced DSB repair . . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
Previous reports have suggested a connection between reduced levels of the catalytic subunit of DNA dependent protein kinases ( DNA PKcs ) , a component of the nonhomologous DNA double strand breaks end joining system , and a reduction in ATM . ^^^ We studied this possible connection in other DNA PKcs deficient cell types , and following knockdown of DNA PKcs with small interfering RNA , Chinese hamster ovary V 3 cells , lacking DNA PKcs , had reduced levels of ATM and hSMG 1 , but both were restored after transfection with PRKDC . ^^^ Reduction of ATM in a human glioma cell line lacking DNA PKcs was accompanied by defective signaling through downstream substrates , post irradiation . ^^^ Only after the DNA PKcs reduction occurred was a reduction in ATM mRNA observed , beginning 2 days post transfection . ^^^ The amount of ATM began to decline , starting about 3 days post treatment , then it declined to levels comparable to DNA PKcs . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
This review article focuses on recent findings related to the initial recognition of double strand breaks by ATM and MRN , as well as a DNA dependent protein kinase complex consisting of the heterodimer Ku70 / Ku80 and its catalytic subunit DNA PKcs , another member of PIKK . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
Wortmannin stimulated the decatenating activity of topoisomerase 2 , promoted etoposide induced accumulation of DNA double strand breaks , shifted the specificity for cell killing by etoposide from the S to G 1 phase of the cell cycle , and potentiated the cytotoxicity of etoposide through two mechanisms . ( a ) Sensitization to high , micromolar amounts of etoposide required integrity of the nonhomologous end joining repair pathway . ( b ) Wortmannin dramatically increased the susceptibility to low , submicromolar amounts of etoposide in a large fraction of the cell population irrespective of the status of ATM , Ku 86 , and DNA PKCS . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
Subnuclear microbeam irradiation studies confirm p53Ser15 is recruited to sites of DNA damage containing gamma H2AX , ATM ( Ser 1981 ) , and DNA PKcs ( Thr 2609 ) in vivo . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
The quickly reacting proteins of human cells include proteinkinases from the family of phophatidylinositol 3 kinase related proteinkinases : ataxia teleangiectasia mutated ( ATM ) , ataxia teleangiectasia and Rad 3 related ( ATR ) and catalytic subunit of DNA dependant proteinkinase ( DNA PKcs ) . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
DNA damage induces histone H2AX phosphorylation by the phosphoinositide 3 kinase like kinases ATM , ATR and DNA PKcs . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
The FATC domains of PIKK proteins are functionally equivalent and participate in the Tip 60 dependent activation of DNA PKcs and ATM . ^^^ Members of the phosphatidylinositol 3 kinase related kinase ( PIKK ) family , including the ATM , DNA PKcs , Atr , and Trrap proteins , function in signal transduction pathways that activate the DNA damage response . ^^^ Replacement of the FATC domain of ATM with the FATC domains of DNA PKcs , Atr , or Trrap restored the activation of ATM and its association with Tip 60 . ^^^ These results indicate that the FATC domains of DNA PKcs , Atr , Trrap , and ATM are functionally equivalent . ^^^ Further , the ability of Tip 60 to regulate the activation of both ATM and DNA PKcs in response to DNA damage demonstrates that Tip 60 is a key component of the DNA damage signaling network . . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
To identify additional proteins required for the recruitment of BLM , we examined the recruitment of BLM in various mutants generated from chicken DT 40 cells and found that the early accumulation of BLM was not dependent on the presence of ATM , RAD 17 , DNA PKcs , NBS 1 , XRCC 3 , RAD 52 , RAD 54 , or WRN . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
Recent work has identified DNA polymerase epsilon , Ku , and proteins such as DNA PKcs , Mec 1 and Tel 1 as key players in DNA damage recognition pathways . ^^^
Interacting proteins: Q13315 and P78527 Pubmed SVM Score :0.0
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