| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| The second phase of cleavage ( 50 min ) involved PARP , U 1 70kDa and DNA PKcs , all substrates of the CPP 32 like proteases . ^^^ |
|
| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| Furthermore , DNA PKcs was cleaved in vitro by purified apopain ( CPP 32 ) , but not IL 1beta converting enzyme . ^^^ |
|
| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| Both DNA PKcs and PARP are also substrates for caspase 3 like activities . ^^^ |
|
| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| Moreover , the mutants , but not DBD , inhibit the cleavage of DNA PKcs , suggesting inhibition of activation of caspase 3 . ^^^ |
|
| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| Increased recruitment of cells undergoing apoptosis was associated with enhanced anti CD 95 induced proteolytic cleavage of the most receptor proximal cysteine protease caspase 8 , subsequent cleavage and activation of the machinery protease caspase 3 , and cleavage of the caspase substrates DNA dependent protein kinase catalytic subunit , poly ( ADP ribose ) polymerase and lamin B 1 . ^^^ |
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| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| In addition , combined treatment of cells with RA plus 8 Cl cAMP resulted in the release of cytochrome c , loss in mitochondrial membrane potential and activation of caspase 3 followed by cleavage of anti poly ( ADP ribose ) polymerase and DNA dependent protein kinase ( catalytic subunit ) . ^^^ |
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| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| Morphology , terminal transferase mediated dUTP digoxigenin nick end labelling ( TUNEL ) and immunohistochemistry for the pro apoptotic enzyme caspase 3 ( CASP 3 ) , for its substrates poly ( ADP ribose ) polymerase ( PARP ) and the DNA dependent protein kinase catalytic subunit ( DNA PKCS ) and for poly ( ADP ribose ) ( PAR ) , an end product of PARP activity , were used to investigate neuronal death in brain infarcts from 15 men and 20 women , aged 46 95 years . ^^^ |
|
| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| It has been demonstrated that the protease caspase 3 , a downstream molecule of the CD 95 pathway , is activated in UV exposed HaCaT cells , and that the DNA dependent protein kinase catalytic subunit ( DNA PKcs ) is cleaved by interleukin 1beta converting enzyme ( ICE ) like protease during apoptosis induced by 10 rays , staurosporine and etoposide . ^^^ |
|
| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| The present studies demonstrate that U 1 70kD and DNA PKcs are excellent substrates for apopain , with cleavage occurring at sites that are highly similar to the cleavage site within PARP . ^^^ |
|
| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| Protease inhibitor data implicated an ICE like protease in the cleavage of DNA PKcs , and it was subsequently shown that the cysteine protease CPP 32 , but not Mch2alpha , ICE or TX , cleaved purified DNA PKcs into three fragments of comparable size with those observed in cells undergoing apoptosis . ^^^ Cleavage sites in DNA PKcs , determined by antibody mapping and microsequencing , were shown to be the same for CPP 32 cleavage and for cleavage catalyzed by extracts from cells undergoing apoptosis . ^^^ |
|
| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| Furthermore , incubation of DNA PKcs with granzyme B did not produce the same cleavage pattern observed in cells undergoing apoptosis and when this substrate was incubated with either CTL extracts or the ICE like protease , CPP 32 . ^^^ Sequence analysis revealed that the cleavage site in DNA PKcs during CTL killing was the same as that when this substrate was exposed to CPP 32 . ^^^ |
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| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| In addition , whereas the DNA PKcs protease activity was not inhibitable by many conventional protease inhibitors , it was inhibitable by a highly selective peptide derived inhibitor of CPP 32 . ^^^ These data strongly suggest that CPP 32 , or a CPP 32 like protease , is responsible for DNA PKcs proteolysis . ^^^ |
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| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| Several of the identified autoantigens are nuclear proteins ( PARP , U 1 70 kDa , and DNA PKcs ) that are substrates for CPP 32 in vitro and in apoptotic cells . ^^^ |
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| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| Tumor necrosis factor or staurosporine induced apoptosis of caspase 3 deficient MCF 7 cells resulted in cleavage of the death substrates PARP , Rb , PAK 2 , DNA PKcs , gelsolin , and DFF 45 , but not alpha fodrin . ^^^ Together our results suggest that caspase 3 is essential for cleavage of alpha fodrin , but dispensable for the cleavage of PARP , Rb , PAK 2 , DNA PKcs , gelsolin , and DFF 45 and imply that one or more caspases other than caspases 2 , 3 , and 7 is activated and plays a crucial role in the cleavage of these substrates in MCF 7 cells . . ^^^ |
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| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| It appears likely that caspase 3 ( CPP 32 ) is involved in this degradation since it was activated only in the apoptosis susceptible cells and the pattern of cleavage of DNA PKcs was similar to that reported previously with recombinant caspase 3 . ^^^ |
|
| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| Here we report that in cells treated with butyric acid , the cleavage of DNA PKcs was paralleled or preceded by the induction of activation of caspase 3 , and these events were inhibited by Bcl 2 overexpression . ^^^ We also demonstrated the redistribution of activated caspase 3 to the nuclear compartment where it locally cleaves DNA PKcs and poly ( ADP ribose ) polymerase , and cleaved fragments were released in the cytosolic compartment . ^^^ |
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| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| To assess the significance of damaged nuclear DNA in autopsy brain tissue in Lewy body disease ( LBD ) , we examined the patterns of expression of two DNA repair enzymes ( PARP and DNA PKCS ) , TUNEL and caspase 3 activation , in sections of midbrain and frontal cortex from nine patients with LBD who had not received L DOPA , and from five neurologically normal controls . ^^^ |
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| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| In parallel , protein expression of DNA PKcs and poly ( ADP ribose ) polymerase ( PARP ) as well as DNA PK and caspase 3 activity were investigated . ^^^ |
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| Interacting proteins: P78527 and P42574 |
Pubmed |
SVM Score :0.0 |
| Further , since proteasome inhibition has been shown to activate caspase 3 , which is involved in apoptosis , and caspase 3 can cleave DNA PKcs , which is involved in DNA double strand repair , the hypothesis was tested that caspase 3 activation was essential for both apoptosis and radiosensitization following proteasome inhibition . ^^^ Cell cycle distribution , apoptosis , caspase 3 activity , DNA PKcs protein levels and DNA PK activity were monitored . ^^^ |
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