| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :1.1281161 |
| We show that the extreme C terminus of Ku 80 , specifically the final 12 amino acid residues , mediates a highly specific interaction with DNA PKcs . 1.1281161^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.51594186 |
| Ku 86 forms complexes with DNA PKcs and activates kinase activity , but Ku86v neither binds DNA PKcs nor activates kinase activity . 0.51594186^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.66439852 |
| Ku 70 and Ku 86 together with DNA PKcs form the DNA dependent protein kinase ( DNA PK ) complex that is involved in DNA double strand break repair by nonhomologous end joining . 0.66439852^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.51938161 |
| Expression of DNA PKcs positively correlated with both Ku 86 and Ku 70 , and a statistically significant correlation between the Ku subunits was also found . 0.51938161^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| DNA dependent protein kinase ( DNA PK ) consists of three polypeptide subunits : Ku 70 , Ku 80 , and the DNA PK catalytic subunit ( DNA PKcs ) . ^^^ Mammalian mutants deficient in either Ku 80 or DNA PKcs function have been shown to be lacking in DNA double strand break repair and 5 ( D ) J recombination , respectively . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| We have analyzed expression of Ku 70 , Ku 80 and DNA PKcs upon induction of differentiation in preB cells derived from wild type , severe combined immunodeficiency ( SCID ) and Rag 2 / mice . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| DNA dependent protein kinase ( DNA PK ) consists of a 460 kDa subunit that contains the catalytic domain ( DNA PKcs ) complexed with two polypeptides of 70 kDa and 80 kDa ( Ku 70 and Ku 80 ) which comprise the Ku autoantigen . ^^^ DNA dependent protein kinase ( DNA PK ) consists of a 460 kDa subunit that contains the catalytic domain ( DNA PKcs ) complexed with two polypeptides of 70 kDa and 80 kDa ( Ku 70 and Ku 80 ) which comprise the Ku autoantigen . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| The DNA dependent protein kinase ( DNA PK ) is a trimeric enzyme consisting of a 460 kDa catalytic subunit ( DNA PKcs ) and a heterodimeric regulatory complex called Ku , which is comprised of 70 ( Ku 70 ) and 86 ( Ku 80 ) kDa subunits . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Two of these are components of the DNA dependent protein kinase ( DNA PK ) and include Ku 80 and DNA PK catalytic subunit ( DNA PKcs ) ; the third , XRCC 4 , is a protein of unknown function . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| The DNA PKcs gene encodes the 465 kDa catalytic subunit of DNA dependent protein kinase ( DNA PK ) , which associates with heterodimeric autoantigens Ku 70 and Ku 80 and exhibits protein kinase activity depending on DNA double strand breaks . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Ku 70 , Ku 80 , and DNA PKcs are subunits of the DNA dependent protein kinase ( DNA PK ) , an enzyme implicated in DNA double stranded break repair and 5 ( D ) J recombination . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| We demonstrate here that the products of the catalytic subunit of DNA dependent protein kinase ( DNA PKcs ) and its regulatory subunits ( Ku 70 and Ku 80 ) are present at normal levels and possess functional activity . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Reduced joining of DNA double strand breaks with an abnormal mutation spectrum in rodent mutants of DNA PKcs and Ku 80 . ^^^ MATERIALS AND METHODS : The efficiency and fidelity of the joining of linear plasmids by DNA PKcs defective mouse cells ( SCID ) and Ku 80 defective Chinese hamster ovary cells ( xrs 6 ) was measured using either linear or circular replicating shuttle vector pZ 189 . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Interestingly , either Ku 70 or Ku 80 deficient cell lines , but not mutant cell lines of the DNA PK catalytic sub unit ( DNA PKcs ) , were found to be hypersensitive to the effects of etoposide VP 16 . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| The DNA dependent protein kinase is a mammalian protein complex composed of Ku 70 , Ku 80 , and DNA PKcs subunits that has been implicated in DNA double strand break repair and 5 ( D ) J recombination . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| The DNA dependent protein kinase ( DNA PK ) consists of Ku 70 , Ku 80 , and a large catalytic subunit , DNA PKcs . ^^^ We conclude that Ku 70 and Ku 80 may have functions in 5 ( D ) J recombination and DNA repair that are independent of DNA PKcs . . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Mutations in any of the three subunits of this protein kinase ( Ku 70 , Ku 80 and DNA PKcs ) lead to sensitivity to ionizing radiation ( IR ) and to DNA double strand breaks , and 5 ( D ) J recombination product formation defects . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| From the analysis of defective rodent mutants , four proteins ( Ku 70 , Ku 80 , DNA PKcs and Xrcc 4 ) that function in this process in mammalian cells have been identified . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NF 90 and NF 45 associate with the DNA dependent protein kinase catalytic subunit and the DNA targeting subunits Ku 80 and Ku 70 ( N . ^^^ The purine box transcriptional regulator in 16HBE cells likely comprises NF 45 , NF 90 , Ku 80 , Ku 70 , and the DNA dependent protein kinase catalytic subunit . . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| The DNA dependent protein kinase ( DNA PK ) consists of a heterodimer DNA binding complex , Ku 70 and Ku 80 , and a large catalytic subunit , DNA PKcs . ^^^ In contrast to the Ku 70 / and Ku 80 / phenotype , DNA PKcs null mice are blocked for 5 ( D ) J coding but not for signal end joint formation . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| PURPOSE : The heat sensitivity of DNA PK activity in hybrid cells and the possible restoration of this activity with extracts from scid cells ( defective in DNA PKcs ) , sxi 3 cells ( defective in Ku 80 ) and V 794 ( sxi 3 parental wild type cells ) was analysed . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| To gain insight into the function of DNA PK in this process we have carried out a mutation analysis of Ku 80 and DNA PKcs . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| The C terminus of Ku 80 activates the DNA dependent protein kinase catalytic subunit . ^^^ We have carried out a 3 ' deletion analysis of Ku 80 , the larger subunit of Ku , and shown that the C terminal 178 amino acid residues are dispensable for DNA end binding activity but are required for efficient interaction of Ku with DNA PKcs . ^^^ These cells have therefore acquired the phenotype of mouse SCID cells despite expressing DNA PKcs protein , suggesting that an interaction between DNA PKcs and Ku , involving the C terminal region of Ku 80 , is required for DNA double strand break rejoining and coding but not signal joint formation . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Aside from DNA PK , XRCC 4 and DNA ligase 4 , other proteins which play a role ( s ) in this repair pathway remain unknown ; DNA PK contains a catalytic subunit ( DNA PKcs ) and a DNA binding subunit ( Ku 70 and Ku 80 ) . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| We report that activation of DNA PKcs and the repression of MMTV transcription from NRE 1 are dependent upon Ku conformation , the manipulation of DNA structure by Ku , and the contact of Ku 80 with DNA . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| To determine the distribution of DNA PK in human tissues , we assayed paraffin embedded sections of normal and cancerous tissues for DNA PKcs and Ku 80 by immunohistochemistry . ^^^ DNA PKcs and Ku 80 expression was usually parallel , but both were expressed in a highly cell and tissue specific manner . ^^^ Neither protein was consistently expressed in liver nor in resting mammary epithelium , but lactating breast epithelium was strongly positive for DNA PKcs and Ku 80 . ^^^ Most cancerous tissues were consistently positive for DNA PKcs and Ku 80 , except invasive carcinoma of the breast . ^^^ DNA PKcs , Ku 80 , and Ku 70 mRNAs were expressed in all normal tissues with relatively little variation in levels . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Protein levels of the DNA PK components Ku 70 , Ku 80 , and DNA PKcs were monitored by immunoblotting . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Targeted disruption of the genes encoding Ku 70 and Ku 80 has identified significant differences between Ku deficient mice and DNA PKcs deficient mice . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| To test this hypothesis further , the ability of heat to enhance the radiosensitivity of cells proficient or deficient in either Ku 80 or the DNA PK catalytic subunit ( DNA PKcs ) was investigated . ^^^ In cells of two Ku 80 deficient and two DNA PKcs deficient and double strand break deficient cell lines , the extent of radiosensitization by heat was not reduced compared to that in both their isogenic gene complemented counterparts as well as to that in their parental cells . ^^^ Thus radiosensitization by hyperthermia can be obtained irrespective of the Ku 80 or DNA PKcs status in cells . ^^^ Therefore , Ku 80 or DNA PKcs and hence nonhomologous DSB end joining do not play a crucial role in the enhancement of cellular radiosensitivity by hyperthermia . . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Using different antibodies to immunoprecipitate the individual components of the DNA PK complex ( DNA PKcs , Ku 70 , and Ku 80 ) , it was shown that APA SG caused a destabilization of the trimeric holoenzyme complex by dissociating the catalytic subunit from the Ku heterodimer . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| DNA PK is a nuclear serine / threonine kinase that comprises three components : a catlytic subunit ( DNA PKCS ) and two regulatory subunits , DNA binding proteins , Ku 80 and Ku 70 . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Using indirect immunoprecipitation , we found that throughout the cell cycle , Ku 70 and Ku 80 were present as heterodimers , some in complex with DNA PKcs . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Ku 70 and Ku 80 have been shown to form a heterodimeric complex that can bind tightly to free DNA ends and activate the protein kinase DNA PKcs . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Any defect in one of the known components of the DNA repair / V ( D ) J recombination machinery ( Ku 70 , Ku 80 , DNA PKcs , XRCC 4 and DNA ligase 4 ) leads to abortion of the 5 ( D ) J rearrangement process , early block in both T and B cell maturation , and ultimately to severe combined immune deficiency ( SCID ) in several animal models . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Using Western blots we measured , in non irradiated cells , the basal expression levels of ATM , Rad 1 and Hus 1 , involved in the control of cellular DNA damage checkpoints , together with DNA PKcs , Ku 70 , Ku 80 ; XRCC 4 , ligaseIV and Rad 51 , involved in radiation induced DSB repair . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Mammalian nonhomologous DNA end joining employs Ku 70 , Ku 80 , DNA dependent protein kinase catalytic subunit ( DNA PKcs ) , XRCC 4 , and DNA ligase 4 ( Lig 4 ) . ^^^ Herein , we show that Ku 70 and Ku 80 deficiency but not DNA PKcs deficiency results in dramatically increased death of developing embryonic neurons in mice . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Its regulatory subunit Ku ( dimer of the Ku 70 and Ku 80 protein ) binds to DNA and recruits the kinase catalytic sub unit , DNA PKcs . ^^^ We show here that three different strains deficient in either the Ku 80 ( xrs 6 ) or DNA PKcs ( 5 3 , scid ) component of DNA PK are markedly sensitive ( 3 . 5 to 5 fold ) to a group of DNA cross linking agents , the nitrogen mustards ( NMs ) ( melphalan and mechlorethamine ) as compared to their parental cell line . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Expression of DNA dependent protein kinase catalytic subunit and Ku 80 in developing human brains : implication of DNA repair in neurogenesis . ^^^ We investigated expression of DNA dependent protein kinase catalytic subunit ( DNA PKcs ) and Ku 80 in developing human brain , both of which have been suggested to be involved in the repair of DNA double strand break ( DSB ) . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Here , we show that Ku 70 , Ku 80 , and DNA PKcs , with which Ku interacts , associate in vivo with telomeric DNA in several human cell types , and we show that these associations are not significantly affected by DNA damaging agents . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| DNA PK comprises a catalytic subunit ( DNA PKcs ) and a DNA binding Ku 70 and Ku 80 heterodimer . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| DNA dependent protein kinase ( DNA PK ) , composed of Ku 70 , Ku 80 , and the catalytic subunit ( DNA PKcs ) , is involved in repairing double strand breaks ( DSBs ) by nonhomologous end joining ( NHEJ ) . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| After exposure to the same single doses , the mRNA levels of DNA PK catalytic subunit ( PKcs ) , Ku 70 , and Ku 80 were similar . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| It is a three protein complex consisting of a 470 kDa catalytic subunit ( DNA PKcs ) and the regulatory DNA binding subunits , Ku heterodimer ( Ku 70 and Ku 80 ) . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NHEJ can be catalyzed by mammalian whole cell extract in a reaction dependent upon DNA ligase 4 , Xrcc 4 , Ku 70 , Ku 80 , and DNA PKcs . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| DNA PK is composed of a large catalytic subunit ( DNA PKcs ) and a heterodimer of Ku 70 and Ku 80 that assemble on the ends of double stranded DNA to form an active serine / threonine protein kinase complex . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| LOH was identified in the specific coding region of DNA PK in 39 . 29 % for the DNA PK catalytic subunit ( cs ) , 17 . 86 % for Ku 70 , and only 7 . 14 % for Ku 80 . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| DNA PK is composed of a large catalytic subunit , DNA PKcs , and a heterodimer of Ku 70 and Ku 80 subunits . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| A synthesized peptide representing the C terminus of Ku 80 ( HNI 38 ) selectively targeted and disrupted interaction between Ku complex and the catalytic subunit of DNA dependent protein kinase ( DNA PKcs ) as well as the DNA binding activity of Ku that led to the inhibition of DNA PK activity and reduction in double stranded DNA break ( dsb ) repair activity . ^^^ Together , this study not only validates the involvement of the C terminus of Ku 80 in Ku ' s DNA termini binding and interaction with DNA PKcs , but also a supports physiological role for DNA PK in IR or chemotherapy drug resistance of cancer cells . . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : To examine the expression of DNA dependent protein kinase ( DNA PK ) ; including DNA PKcs , Ku 70 and Ku 80 in human neuroectodermal tumor cells with or without retroviral DNA integration . ^^^ RESULTS : The expression of DNA PKcs was similar in the cells with or without retroviral DNA integration , but the expression of Ku ( both mRNA and protein for Ku 70 and only protein for Ku 80 ) was higher in cells with retroviral DNA integration than in cells without retroviral DNA integration . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| In analyzing the DNA repair capacity of C 70 and C 200 , an altered expression of the DNA dependent protein kinase ( DNA PK ) complex ( catalytic subunit DNA PKcs , and the heterodimers Ku 70 and Ku 80 ) was found . ^^^ In C 70 and C 200 cells , DNA PKcs was overexpressed at both the transcript and protein levels , whereas amounts of Ku 70 and Ku 80 were higher only at the level of protein expression . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Ku 70 protein , cooperating with Ku 80 and DNA dependent protein kinase ( DNA PK ) catalytic subunit ( DNA PKcs ) , is involved in DNA double strand break ( DNA DSB ) repair and 5 ( D ) J recombination . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Cells of mouse knockout cell lines for Ku 80 ( now known as Xrcc 5 ) , Ku 70 ( now known as G22p1 ) , DNA PKcs ( now known as Prkdc ) and PARP ( now known as Adprt ) were synchronized in G 1 phase and exposed to very low fluences of alpha particles . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Chinese hamster ovary ( CHO K 1 ) cell line and two of its DNA double strand break ( DSB ) repair deficient mutant cell lines , xrs 5 ( Ku 80 mutant ) and irs 20 ( DNA PKcs mutant ) , were treated with various concentrations of sodium arsenite for 2 . 5h , and the colony forming abilities were studied . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Detailed examination indicates that the patient is defective neither in the known factors involved in NHEJ in mammals ( Ku 70 , Ku 80 , DNA dependent protein kinase catalytic subunit , Xrcc 4 , DNA ligase 4 , or Artemis ) nor in the Mre11 / Rad50 / Nbs1 complex , whose homologue in Saccharomyces cerevisiae functions in NHEJ . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| The intracellular distribution and amount of the DNA dependent protein kinase ( DNA PK ) complex components ( ku 70 , ku 80 , DNA PKcs ) , which are involved in the recognition and repair of DNA breaks caused by ionizing radiations , 5 ( D ) J recombination and isotype switching , was assessed in nai ; ve and memory T cells from young subjects . ^^^ While the expression of ku 70 and ku 80 was at comparable levels in both T cell subsets , DNA PKcs , phosphorylated ku 80 , and DNA binding of ku 70 / 80 were mostly evident in nai ; ve but negligible or absent in memory T cells . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Because single nucleotide polymorphisms ( SNPs ) are the most subtle genetic variation in the genome , to examine these hypotheses , we have genotyped 30 SNPs in all five NHEJ genes ( Ku 70 , Ku 80 , DNA PKcs , Ligase 4 , and XRCC 4 ) in 254 primary breast cancer patients and 379 healthy controls . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| DNA PK is a nuclear protein with serine / threonine kinase activity and forms a complex consisting of the DNA PKcs and a heterodimer of Ku 70 and Ku 80 proteins . ^^^ In this study , we evaluated the relationship between expression levels of DNA PKcs , Ku 70 and Ku 80 proteins and radiation sensitivity in oral squamous cell carcinoma ( OSCC ) cell lines and in OSCC patients treated with preoperative radiation therapy . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| DNA dependent protein kinase ( DNA PK ) , composed of Ku 70 , Ku 80 , and the catalytic subunit ( DNA PKcs ) , is involved in double strand break ( DSB ) repair by non homologous end joining ( NHEJ ) . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| However , these structures lack a region of ca 190 residues from the C terminal region ( CTR ) of the Ku 86 subunit ( also known as Lupus Ku autoantigen p 86 , Ku 80 , or XRCC 5 ) that includes the extreme C terminal tail that is reported to be sufficient for DNA PKcs binding . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Exposure of LM 217 and MDA MB 468 cells to suramin did not affect the level of Ku 70 ( G22P1 ) or Ku 80 ( XRCC 5 ) , but it increased the level of DNA PKcs ( PRKDC ) . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Here we identify related , conserved carboxy terminal motifs in human Nbs 1 , ATRIP and Ku 80 proteins that are required for their interaction with ATM , ATR and DNA PKcs , respectively . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVES : Ku , a heterodimer of KU 70 and Ku 80 that binds to double strand DNA breaks ( DSBs ) and activates the catalytic subunit ( DNA PKcs ) when DNA is bound , is essential in DSB repair and 5 ( D ) J recombination . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| LOH was recurrently observed for 8 of the 9 studied genes ( KU 70 , KU 80 , XRCC 4 , LIG 4 , Artemis , MRE 11 , RAD 50 , NBS 1 ) but not for DNA PKcs . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| The accumulation of WRN at double strand breaks was rapid , persisted for many hours , and occurred in the absence of several known interacting proteins including polymerase beta , poly ( ADP ribose ) polymerase 1 ( PARP 1 ) , Ku 80 , DNA dependent protein kinase ( DNA PKcs ) , NBS 1 and histone H2AX . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Dictyostelium Ku 80 contains a conserved domain previously implicated in recruiting DNA PKcs to DNA and consistent with this observation , we have identified DNA PKcs in the Dictyostelium genome . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Down regulation of PARP 1 , but not of Ku 80 or DNA PKcs ' , results in higher gene targeting efficiency . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| In addition , PARP 1 / cells are shown to be sensitive to the DNA PK inhibitor NU 7026 and DNA PKcs or Ku 80 defective cells shown to be sensitive to PARP inhibitors . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Esophageal cancer tissues and adjacent normal mucosae in 13 patients with primary esophageal cancer were examined for quantitative differences in DNA dependent protein kinase ( DNA PK ) activity and for expressions of Ku 70 , Ku 80 and DNA PKcs proteins by Western blotting and immunohistochemistry . ^^^ Protein levels of Ku 70 , Ku 80 and DNA PKcs correlated with DNA PK activities in the tumor tissues . ^^^ Immunohistochemical analysis revealed that Ku 70 , Ku 80 and DNA PKcs located predominantly in the nuclei in both the tumor tissues and normal mucosae . ^^^ In the normal epithelium , Ku 70 , Ku 80 and DNA PKcs were expressed only in the nuclei of the basal cell layers and not in those of the lumenal cell layers . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Three proteins known to play a critical role in mammalian DNA double strand break repair and lymphoid 5 ( D ) J recombination are the autoantigens Ku 86 and Ku 70 and a 465 kDa serine / threonine protein kinase catalytic subunit ( DNA PKcs ) . ^^^ PK assays demonstrated that the Ku complexes containing variant Ku 86 had a greatly reduced ability to interact with DNA PKcs and that consequently HL 60 cells had severely diminished DNA . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Recent studies suggest that the SCID defect lies in the gene encoding the catalytic subunit of DNA dependent protein kinase ( DNA PK ; refs 6 8 ) , a nuclear protein made up of the Ku 70 and Ku 86 subunits as well as the large catalytic subunit , DNA PKcs . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Intermolecular ligation was found to be accurate and to depend on DNA ligase IV / Xrcc4 and requires Ku 70 , Ku 86 , and DNA PKcs , the three subunits of the DNA activated protein kinase DNA PK . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Previous studies have suggested that ionizing radiation causes irreparable DNA double strand breaks in mice and cell lines harboring mutations in any of the three subunits of DNA dependent protein kinase ( DNA PK ) ( the catalytic subunit , DNA PKcs , or one of the DNA binding subunits , Ku 70 or Ku 86 ) . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| DNA PK plays a major role in the repair of double strand breaks induced in DNA after exposure to ionizing radiation as shown by the extreme radiosensitivity of cells with mutations in Ku 86 , Ku 70 or DNA PKcs genes . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Ku 86 together with Ku 70 , DNA PKcs , XRCC 4 and DNA ligase 4 forms a complex involved in repairing DNA double strand breaks ( DSB ) in mammals . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| DNA dependent protein kinase ( DNA PK ) , consisting of a catalytic subunit ( DNA PKcs ) and the Ku 70 and Ku 86 proteins , participates in the repair of DNA double strand breaks ( DSBs ) . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Blimp 1 also inhibited immunoglobulin class switching by blocking expression of AID , Ku 70 , Ku 86 , DNA PKcs , and STAT 6 . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| The results clearly show that the antibody recognizes the large subunit ( Ku 86 ) of the Ku autoantigen , the regulatory component of the DNA PKcs . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| The gels were then processed for Western blotting using the Ku 70 , Ku 86 and DNA PKcs antibodies . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| All components of the double stranded DNA break ( DSB ) repair complex DNA dependent protein kinase ( DNA PK ) , including Ku 70 , Ku 86 , and DNA PK catalytic subunit ( DNA PKcs ) , were found in the radiosensitive spermatogonia . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Furthermore , survival of patients with high expression of DNA PKcs or Ku 86 and low MDM 2 levels was significantly better when compared with survival of patients with low DNA PKcs or Ku 86 and high MDM 2 ( p = . 0017 and p = . 0034 , respectively ) . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Six components function in this pathway , of which three ( Ku 70 , Ku 86 , and DNA PKcs ) constitute a protein complex known as DNA dependent protein kinase ( DNA PK ) . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| TdT synthesizes the N region in concert with many proteins including DNA PKcs , Ku 70 and Ku 86 . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| The decreased kinase activity of DNA PKcs was not due to a change in its amount or the levels of Ku 70 and Ku 86 , their subcellular distribution , cell cycle progression or caspase mediated degradation of DNA PK . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| In addition , by using a protease resistant whole cell assay , both Ku 86 and DNA PKcs could be demonstrated in PMN , suggesting the previously reported absence in PMN of DNA PK to be an artefact . ^^^ The levels of Ku 86 and DNA PKcs were much reduced in PMN , as compared with that of the lymphocytes , whereas HL 60 displayed a markedly elevated DNA PK concentration . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Impact of telomerase ablation on organismal viability , aging , and tumorigenesis in mice lacking the DNA repair proteins PARP 1 , Ku 86 , or DNA PKcs . ^^^ The DNA repair proteins poly ( ADP ribose ) polymerase 1 ( PARP 1 ) , Ku 86 , and catalytic subunit of DNA PK ( DNA PKcs ) have been involved in telomere metabolism . ^^^ To genetically dissect the impact of these activities on telomere function , as well as organismal cancer and aging , we have generated mice doubly deficient for both telomerase and any of the mentioned DNA repair proteins , PARP 1 , Ku 86 , or DNA PKcs . ^^^ In contrast , mice doubly deficient for telomerase and either Ku 86 or DNA PKcs manifest accelerated loss of organismal viability compared with single telomerase deficient mice . ^^^ |
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| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Moreover , NaB significantly reduced the expression of the repair related genes Ku 70 and Ku 86 and DNA dependent protein kinase catalytic subunit in melanoma cells at the protein and mRNA levels . ^^^ |
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| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| We demonstrate that cells deficient in Ku 86 , but not cells deficient in DNA PKcs ( the catalytic subunit of DNA PK ) , are hypersensitive to high NaCl as manifested by profound inhibition of proliferation , aberrant mitosis , and increased chromosomal fragmentation . ^^^ |
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| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Wortmannin stimulated the decatenating activity of topoisomerase 2 , promoted etoposide induced accumulation of DNA double strand breaks , shifted the specificity for cell killing by etoposide from the S to G 1 phase of the cell cycle , and potentiated the cytotoxicity of etoposide through two mechanisms . ( a ) Sensitization to high , micromolar amounts of etoposide required integrity of the nonhomologous end joining repair pathway . ( b ) Wortmannin dramatically increased the susceptibility to low , submicromolar amounts of etoposide in a large fraction of the cell population irrespective of the status of ATM , Ku 86 , and DNA PKCS . ^^^ |
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| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| There was no change in the protein levels of DNA PKcs , Ku 70 , or Ku 86 . ^^^ |
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| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Radiosensitive cell lines derived from 10 ray cross complementing group 5 ( XRCC 5 ) , SCID mice and a human glioma cell line lack components of the DNA dependent protein kinase , DNA PK , suggesting that DNA PK plays an important role in DNA double strand break repair . ^^^ |
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| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Genes essential for the end joining reaction include : XRCC 4 encoding a protein of unknown enzymatic function ; XRCC 5 and XRCC 6 encoding 86 and 70 kDa subunits of the Ku autoantigen , a DNA end binding protein that is also the regulatory subunit of DNA dependent protein kinase ( DNA PK ) ; and XRCC 7 encoding the catalytic subunit ( DNA PKcs ) of DNA PK . ^^^ |
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| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| DNA PK is a heterotrimeric protein complex comprised of two components : a large catalytic subunit , Prkdc , with serine / threonine kinase activity and a DNA targeting component , G22p1 and Xrcc 5 . ^^^ |
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| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| The presence of PRKDC , G22p1 and Xrcc 5 proteins was determined by Western blotting and a kinase assay used to measure DNA PK complex activity . ^^^ |
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| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| Strikingly , we found that doxazosin induces deregulation of genes implicated in DNA replication and repair , such as GADD45A , XRCC 5 and PRKDC . ^^^ These facts , together with the demonstration of the ability of doxazosin to bind DNA , allowed us to propose a novel mechanism of action for doxazosin in prostate cancer cells that implies DNA damage mediated apoptosis by down regulation of XRCC 5 and PRKDC genes . . ^^^ |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13010 and P78527 |
Pubmed |
SVM Score :0.0 |
| NA |
|