| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :1.0042794 |
| Ku 70 and Ku 86 together with DNA PKcs form the DNA dependent protein kinase ( DNA PK ) complex that is involved in DNA double strand break repair by nonhomologous end joining . 1.0042794^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| After exposure to the same single doses , the mRNA levels of DNA PK catalytic subunit ( PKcs ) , Ku 70 , and Ku 80 were similar . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| DNA PKcs , Ku 80 , and Ku 70 mRNAs were expressed in all normal tissues with relatively little variation in levels . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Blimp 1 also inhibited immunoglobulin class switching by blocking expression of AID , Ku 70 , Ku 86 , DNA PKcs , and STAT 6 . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| The gels were then processed for Western blotting using the Ku 70 , Ku 86 and DNA PKcs antibodies . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| DNA PK is composed of a large catalytic subunit , DNA PKcs , and a heterodimer of Ku 70 and Ku 80 subunits . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| TdT synthesizes the N region in concert with many proteins including DNA PKcs , Ku 70 and Ku 86 . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Ku 70 , DNA PKcs , DNA Ligase 4 , Xrcc 4 ) were observed . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| There was no change in the protein levels of DNA PKcs , Ku 70 , or Ku 86 . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| DNA dependent protein kinase ( DNA PK ) consists of a 460 kDa subunit that contains the catalytic domain ( DNA PKcs ) complexed with two polypeptides of 70 kDa and 80 kDa ( Ku 70 and Ku 80 ) which comprise the Ku autoantigen . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NF 90 and NF 45 associate with the DNA dependent protein kinase catalytic subunit and the DNA targeting subunits Ku 80 and Ku 70 ( N . ^^^ The purine box transcriptional regulator in 16HBE cells likely comprises NF 45 , NF 90 , Ku 80 , Ku 70 , and the DNA dependent protein kinase catalytic subunit . . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Mammalian nonhomologous DNA end joining employs Ku 70 , Ku 80 , DNA dependent protein kinase catalytic subunit ( DNA PKcs ) , XRCC 4 , and DNA ligase 4 ( Lig 4 ) . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Detailed examination indicates that the patient is defective neither in the known factors involved in NHEJ in mammals ( Ku 70 , Ku 80 , DNA dependent protein kinase catalytic subunit , Xrcc 4 , DNA ligase 4 , or Artemis ) nor in the Mre11 / Rad50 / Nbs1 complex , whose homologue in Saccharomyces cerevisiae functions in NHEJ . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Unexpectedly , however , we found that nonhomologous end joining ( NHEJ ) , another DSB repair pathway , has no positive role in the relevant repair ; notably , DT 40 cell mutants lacking either of the NHEJ factors ( namely , Ku 70 , DNA dependent protein kinase catalytic subunit , and DNA ligase 4 ) were resistant to killing by CPT . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Moreover , NaB significantly reduced the expression of the repair related genes Ku 70 and Ku 86 and DNA dependent protein kinase catalytic subunit in melanoma cells at the protein and mRNA levels . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| DNA dependent protein kinase ( DNA PK ) consists of three polypeptide subunits : Ku 70 , Ku 80 , and the DNA PK catalytic subunit ( DNA PKcs ) . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| LOH was identified in the specific coding region of DNA PK in 39 . 29 % for the DNA PK catalytic subunit ( cs ) , 17 . 86 % for Ku 70 , and only 7 . 14 % for Ku 80 . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| All components of the double stranded DNA break ( DSB ) repair complex DNA dependent protein kinase ( DNA PK ) , including Ku 70 , Ku 86 , and DNA PK catalytic subunit ( DNA PKcs ) , were found in the radiosensitive spermatogonia . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Three proteins known to play a critical role in mammalian DNA double strand break repair and lymphoid 5 ( D ) J recombination are the autoantigens Ku 86 and Ku 70 and a 465 kDa serine / threonine protein kinase catalytic subunit ( DNA PKcs ) . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| We have analyzed expression of Ku 70 , Ku 80 and DNA PKcs upon induction of differentiation in preB cells derived from wild type , severe combined immunodeficiency ( SCID ) and Rag 2 / mice . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| The DNA dependent protein kinase ( DNA PK ) is a trimeric enzyme consisting of a 460 kDa catalytic subunit ( DNA PKcs ) and a heterodimeric regulatory complex called Ku , which is comprised of 70 ( Ku 70 ) and 86 ( Ku 80 ) kDa subunits . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Genes essential for the end joining reaction include : XRCC 4 encoding a protein of unknown enzymatic function ; XRCC 5 and XRCC 6 encoding 86 and 70 kDa subunits of the Ku autoantigen , a DNA end binding protein that is also the regulatory subunit of DNA dependent protein kinase ( DNA PK ) ; and XRCC 7 encoding the catalytic subunit ( DNA PKcs ) of DNA PK . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| The DNA PKcs gene encodes the 465 kDa catalytic subunit of DNA dependent protein kinase ( DNA PK ) , which associates with heterodimeric autoantigens Ku 70 and Ku 80 and exhibits protein kinase activity depending on DNA double strand breaks . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Mutations in any of the three subunits of this protein kinase ( Ku 70 , Ku 80 and DNA PKcs ) lead to sensitivity to ionizing radiation ( IR ) and to DNA double strand breaks , and 5 ( D ) J recombination product formation defects . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Ku 70 , Ku 80 , and DNA PKcs are subunits of the DNA dependent protein kinase ( DNA PK ) , an enzyme implicated in DNA double stranded break repair and 5 ( D ) J recombination . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Recent studies suggest that the SCID defect lies in the gene encoding the catalytic subunit of DNA dependent protein kinase ( DNA PK ; refs 6 8 ) , a nuclear protein made up of the Ku 70 and Ku 86 subunits as well as the large catalytic subunit , DNA PKcs . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| We demonstrate here that the products of the catalytic subunit of DNA dependent protein kinase ( DNA PKcs ) and its regulatory subunits ( Ku 70 and Ku 80 ) are present at normal levels and possess functional activity . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Interestingly , either Ku 70 or Ku 80 deficient cell lines , but not mutant cell lines of the DNA PK catalytic sub unit ( DNA PKcs ) , were found to be hypersensitive to the effects of etoposide VP 16 . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| The DNA dependent protein kinase is a mammalian protein complex composed of Ku 70 , Ku 80 , and DNA PKcs subunits that has been implicated in DNA double strand break repair and 5 ( D ) J recombination . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| The DNA dependent protein kinase ( DNA PK ) consists of Ku 70 , Ku 80 , and a large catalytic subunit , DNA PKcs . ^^^ We conclude that Ku 70 and Ku 80 may have functions in 5 ( D ) J recombination and DNA repair that are independent of DNA PKcs . . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| From the analysis of defective rodent mutants , four proteins ( Ku 70 , Ku 80 , DNA PKcs and Xrcc 4 ) that function in this process in mammalian cells have been identified . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| These factors include components of DNA dependent protein kinase ( DNA PK ) , namely DNA PKcs and the Ku heterodimer , Ku 70 Ku80 , and XRCC 4 . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Intermolecular ligation was found to be accurate and to depend on DNA ligase IV / Xrcc4 and requires Ku 70 , Ku 86 , and DNA PKcs , the three subunits of the DNA activated protein kinase DNA PK . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Previous studies have suggested that ionizing radiation causes irreparable DNA double strand breaks in mice and cell lines harboring mutations in any of the three subunits of DNA dependent protein kinase ( DNA PK ) ( the catalytic subunit , DNA PKcs , or one of the DNA binding subunits , Ku 70 or Ku 86 ) . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| The DNA dependent protein kinase ( DNA PK ) consists of a heterodimer DNA binding complex , Ku 70 and Ku 80 , and a large catalytic subunit , DNA PKcs . ^^^ In contrast to the Ku 70 / and Ku 80 / phenotype , DNA PKcs null mice are blocked for 5 ( D ) J coding but not for signal end joint formation . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| When bound to DNA , the Ku 70 / 80 heterodimer enhances the kinase activity of the catalytic subunit of the DNA dependent protein kinase , DNA PKcs . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Aside from DNA PK , XRCC 4 and DNA ligase 4 , other proteins which play a role ( s ) in this repair pathway remain unknown ; DNA PK contains a catalytic subunit ( DNA PKcs ) and a DNA binding subunit ( Ku 70 and Ku 80 ) . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Targeted disruption of the genes encoding Ku 70 and Ku 80 has identified significant differences between Ku deficient mice and DNA PKcs deficient mice . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Using different antibodies to immunoprecipitate the individual components of the DNA PK complex ( DNA PKcs , Ku 70 , and Ku 80 ) , it was shown that APA SG caused a destabilization of the trimeric holoenzyme complex by dissociating the catalytic subunit from the Ku heterodimer . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| DNA PK is a nuclear serine / threonine kinase that comprises three components : a catlytic subunit ( DNA PKCS ) and two regulatory subunits , DNA binding proteins , Ku 80 and Ku 70 . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Using indirect immunoprecipitation , we found that throughout the cell cycle , Ku 70 and Ku 80 were present as heterodimers , some in complex with DNA PKcs . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| DNA PK plays a major role in the repair of double strand breaks induced in DNA after exposure to ionizing radiation as shown by the extreme radiosensitivity of cells with mutations in Ku 86 , Ku 70 or DNA PKcs genes . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Ku 70 and Ku 80 have been shown to form a heterodimeric complex that can bind tightly to free DNA ends and activate the protein kinase DNA PKcs . ^^^ These findings suggest that Ku 70 up regulation provides the cell with a means of assuring either proper DNA repair or an appropriate response to DNA damage independent of DNA PKcs activation . . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Any defect in one of the known components of the DNA repair / V ( D ) J recombination machinery ( Ku 70 , Ku 80 , DNA PKcs , XRCC 4 and DNA ligase 4 ) leads to abortion of the 5 ( D ) J rearrangement process , early block in both T and B cell maturation , and ultimately to severe combined immune deficiency ( SCID ) in several animal models . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Using Western blots we measured , in non irradiated cells , the basal expression levels of ATM , Rad 1 and Hus 1 , involved in the control of cellular DNA damage checkpoints , together with DNA PKcs , Ku 70 , Ku 80 ; XRCC 4 , ligaseIV and Rad 51 , involved in radiation induced DSB repair . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Its regulatory subunit Ku ( dimer of the Ku 70 and Ku 80 protein ) binds to DNA and recruits the kinase catalytic sub unit , DNA PKcs . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Ku is a heterodimer of Ku 70 and Ku 86 that binds to double stranded DNA breaks ( DSBs ) , activates the catalytic subunit ( DNA PKcs ) when DNA is bound , and is essential in DSB repair and 5 ( D ) J recombination . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Ku 86 together with Ku 70 , DNA PKcs , XRCC 4 and DNA ligase 4 forms a complex involved in repairing DNA double strand breaks ( DSB ) in mammals . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Here , we show that Ku 70 , Ku 80 , and DNA PKcs , with which Ku interacts , associate in vivo with telomeric DNA in several human cell types , and we show that these associations are not significantly affected by DNA damaging agents . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| DNA PK comprises a catalytic subunit ( DNA PKcs ) and a DNA binding Ku 70 and Ku 80 heterodimer . ^^^ DNA PKcs deficiency caused a DSB repair defect that was , unexpectedly , suppressed by KU 70 disruption . ^^^ To test whether direct interference by Ku 70 with HR might explain the Ku 70 ( / ) / DNA PKcs ( / / ) radioresistance , we monitored HR activities directly in Ku and DNA PKcs deficient cells . ^^^ Significantly , abrogation of HR activity by targeting RAD 54 in Ku 70 ( / ) or DNA PKcs ( / / ) cells caused extreme radiosensitivity , suggesting that the relative radioresistance seen with loss of Ku 70 was because of HR dependent repair pathways . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| DNA dependent protein kinase ( DNA PK ) , consisting of a catalytic subunit ( DNA PKcs ) and the Ku 70 and Ku 86 proteins , participates in the repair of DNA double strand breaks ( DSBs ) . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| DNA dependent protein kinase ( DNA PK ) , composed of Ku 70 , Ku 80 , and the catalytic subunit ( DNA PKcs ) , is involved in repairing double strand breaks ( DSBs ) by nonhomologous end joining ( NHEJ ) . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| It is a three protein complex consisting of a 470 kDa catalytic subunit ( DNA PKcs ) and the regulatory DNA binding subunits , Ku heterodimer ( Ku 70 and Ku 80 ) . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| NHEJ can be catalyzed by mammalian whole cell extract in a reaction dependent upon DNA ligase 4 , Xrcc 4 , Ku 70 , Ku 80 , and DNA PKcs . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| DNA PK is composed of a large catalytic subunit ( DNA PKcs ) and a heterodimer of Ku 70 and Ku 80 that assemble on the ends of double stranded DNA to form an active serine / threonine protein kinase complex . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : To examine the expression of DNA dependent protein kinase ( DNA PK ) ; including DNA PKcs , Ku 70 and Ku 80 in human neuroectodermal tumor cells with or without retroviral DNA integration . ^^^ RESULTS : The expression of DNA PKcs was similar in the cells with or without retroviral DNA integration , but the expression of Ku ( both mRNA and protein for Ku 70 and only protein for Ku 80 ) was higher in cells with retroviral DNA integration than in cells without retroviral DNA integration . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| In analyzing the DNA repair capacity of C 70 and C 200 , an altered expression of the DNA dependent protein kinase ( DNA PK ) complex ( catalytic subunit DNA PKcs , and the heterodimers Ku 70 and Ku 80 ) was found . ^^^ In C 70 and C 200 cells , DNA PKcs was overexpressed at both the transcript and protein levels , whereas amounts of Ku 70 and Ku 80 were higher only at the level of protein expression . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Ku 70 protein , cooperating with Ku 80 and DNA dependent protein kinase ( DNA PK ) catalytic subunit ( DNA PKcs ) , is involved in DNA double strand break ( DNA DSB ) repair and 5 ( D ) J recombination . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Cells of mouse knockout cell lines for Ku 80 ( now known as Xrcc 5 ) , Ku 70 ( now known as G22p1 ) , DNA PKcs ( now known as Prkdc ) and PARP ( now known as Adprt ) were synchronized in G 1 phase and exposed to very low fluences of alpha particles . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Six components function in this pathway , of which three ( Ku 70 , Ku 86 , and DNA PKcs ) constitute a protein complex known as DNA dependent protein kinase ( DNA PK ) . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| The intracellular distribution and amount of the DNA dependent protein kinase ( DNA PK ) complex components ( ku 70 , ku 80 , DNA PKcs ) , which are involved in the recognition and repair of DNA breaks caused by ionizing radiations , 5 ( D ) J recombination and isotype switching , was assessed in nai ; ve and memory T cells from young subjects . ^^^ While the expression of ku 70 and ku 80 was at comparable levels in both T cell subsets , DNA PKcs , phosphorylated ku 80 , and DNA binding of ku 70 / 80 were mostly evident in nai ; ve but negligible or absent in memory T cells . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Because single nucleotide polymorphisms ( SNPs ) are the most subtle genetic variation in the genome , to examine these hypotheses , we have genotyped 30 SNPs in all five NHEJ genes ( Ku 70 , Ku 80 , DNA PKcs , Ligase 4 , and XRCC 4 ) in 254 primary breast cancer patients and 379 healthy controls . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| DNA PK is a nuclear protein with serine / threonine kinase activity and forms a complex consisting of the DNA PKcs and a heterodimer of Ku 70 and Ku 80 proteins . ^^^ In this study , we evaluated the relationship between expression levels of DNA PKcs , Ku 70 and Ku 80 proteins and radiation sensitivity in oral squamous cell carcinoma ( OSCC ) cell lines and in OSCC patients treated with preoperative radiation therapy . ^^^ Expression of DNA PKcs and Ku 70 after irradiation was increased in the surviving cells of OSCC tissues irradiated preoperatively . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| DNA dependent protein kinase ( DNA PK ) , composed of Ku 70 , Ku 80 , and the catalytic subunit ( DNA PKcs ) , is involved in double strand break ( DSB ) repair by non homologous end joining ( NHEJ ) . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| The decreased kinase activity of DNA PKcs was not due to a change in its amount or the levels of Ku 70 and Ku 86 , their subcellular distribution , cell cycle progression or caspase mediated degradation of DNA PK . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Exposure of LM 217 and MDA MB 468 cells to suramin did not affect the level of Ku 70 ( G22P1 ) or Ku 80 ( XRCC 5 ) , but it increased the level of DNA PKcs ( PRKDC ) . ^^^ |
|
| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : To characterize DNA PKcs and Ku 70 expressions in hepato and cholangio neoplastic tissues and the association with the degree of malignancy and invasiveness of the tumors . ^^^ METHODS : The expression of DNA PKcs and Ku 70 was examined in 47 cases of hepato or cholangio neoplasm by immunohistochemistry . ^^^ In contrast to Ku 70 , a wide variation in expression levels of DNA Pkcs was observed among different types of neoplastic tissues . ^^^ |
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| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVES : Ku , a heterodimer of KU 70 and Ku 80 that binds to double strand DNA breaks ( DSBs ) and activates the catalytic subunit ( DNA PKcs ) when DNA is bound , is essential in DSB repair and 5 ( D ) J recombination . ^^^ |
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| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| LOH was recurrently observed for 8 of the 9 studied genes ( KU 70 , KU 80 , XRCC 4 , LIG 4 , Artemis , MRE 11 , RAD 50 , NBS 1 ) but not for DNA PKcs . ^^^ |
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| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Expressions of Ku 70 and DNA PKcs as prognostic indicators of local control in nasopharyngeal carcinoma . ^^^ PURPOSE : The objective of this study was to determine whether the expressions of the two components of DNA dependent protein kinase , Ku 70 and DNA protein kinase catalytic subunit ( DNA PKcs ) , influence the response to radiotherapy ( RT ) and outcome of treatment of nondisseminated nasopharyngeal carcinoma ( NPC ) in patients who received definitive RT . ^^^ METHODS AND MATERIALS : Sixty six patients with NPC who were treated with radiotherapy alone or with concurrent chemotherapy between June 1995 and December 2001 were divided into groups based on the levels of immunoreactivity for Ku 70 and DNA PKcs in pretreatment biopsy specimens . ^^^ The overexpression of Ku 70 or DNA PKcs groups included patients whose biopsy specimens showed at least 50 % immunopositive tumor cells ; patients in which less than 50 % of the tumor cells in the biopsy tissues were immunopositive were placed in the low Ku 70 and DNA PKcs groups . ^^^ The immunoreactivities for Ku 70 and DNA PKcs were retrospectively compared with the sensitivity of the tumor to radiation and the patterns of therapy failure . ^^^ |
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| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Western blot analysis demonstrated that celecoxib downregulated the expression of Ku 70 protein and inhibited the kinase activity of DNA PKcs , which are involved in the double stranded DNA break repair machinery . ^^^ |
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| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| As expected , 5 ( D ) J recombination was substantially impaired in cells deficient for the NHEJ components Ku 70 , XRCC 4 , and DNA PKcs . ^^^ |
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| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| We found that tumour transformed tissue shows positive immunostaining of DNA PKcs , Ku 86 and Ku 70 , while non neoplastic squamous epithelium and tumour free cervix glands show negative immunoreactivity . ^^^ Expression of DNA PKcs positively correlated with both Ku 86 and Ku 70 , and a statistically significant correlation between the Ku subunits was also found . ^^^ |
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| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Esophageal cancer tissues and adjacent normal mucosae in 13 patients with primary esophageal cancer were examined for quantitative differences in DNA dependent protein kinase ( DNA PK ) activity and for expressions of Ku 70 , Ku 80 and DNA PKcs proteins by Western blotting and immunohistochemistry . ^^^ Protein levels of Ku 70 , Ku 80 and DNA PKcs correlated with DNA PK activities in the tumor tissues . ^^^ Immunohistochemical analysis revealed that Ku 70 , Ku 80 and DNA PKcs located predominantly in the nuclei in both the tumor tissues and normal mucosae . ^^^ In the normal epithelium , Ku 70 , Ku 80 and DNA PKcs were expressed only in the nuclei of the basal cell layers and not in those of the lumenal cell layers . ^^^ |
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| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| Transcript levels for 4 of 6 recombinational repair genes ( XRCC 7 , XRCC 6 , XRCC 1 , and RAD 51 ) were higher in the patient cell lines , though this difference was significant only for XRCC 7 ( P = . 003 ) . ^^^ |
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| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| DNA PK is a heterotrimeric protein complex comprised of two components : a large catalytic subunit , Prkdc , with serine / threonine kinase activity and a DNA targeting component , G22p1 and Xrcc 5 . ^^^ |
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| Interacting proteins: P78527 and P12956 |
Pubmed |
SVM Score :0.0 |
| The presence of PRKDC , G22p1 and Xrcc 5 proteins was determined by Western blotting and a kinase assay used to measure DNA PK complex activity . ^^^ |
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