| Interacting proteins: Q9UPX8 and P78352 |
Pubmed |
SVM Score :0.65849972 |
| Coimmunoprecipitation indicates that Shank , a postsynaptic density protein associated with mGluRs , formed a complex involving PSD 95 ( postsynaptic density 95 ) , NR2B , and Src in the spinal dorsal horn . 0.65849972^^^ |
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| Interacting proteins: Q9UPX8 and P78352 |
Pubmed |
SVM Score :0.0 |
| Shank , a novel family of postsynaptic density proteins that binds to the NMDA receptor / PSD 95 / GKAP complex and cortactin . ^^^ We report a novel family of postsynaptic density ( PSD ) proteins , termed Shank , that binds via its PDZ domain to the C terminus of PSD 95 associated protein GKAP . ^^^ A ternary complex of Shank / GKAP / PSD 95 assembles in heterologous cells and can be coimmunoprecipitated from rat brain . ^^^ Shank may function as a scaffold protein in the PSD , potentially cross linking NMDA receptor / PSD 95 complexes and coupling them to regulators of the actin cytoskeleton . . ^^^ |
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| Interacting proteins: Q9UPX8 and P78352 |
Pubmed |
SVM Score :0.0 |
| Coupling of mGluR / Homer and PSD 95 complexes by the Shank family of postsynaptic density proteins . ^^^ Shank is a recently described family of postsynaptic proteins that function as part of the NMDA receptor associated PSD 95 complex ( Naisbitt et al . , 1999 [ this issue of Neuron ] ) . ^^^ Moreover , Shank clusters mGluR 5 in heterologous cells in the presence of Homer and mediates the coclustering of Homer with PSD 95 / GKAP . ^^^ Thus , Shank may cross link Homer and PSD 95 complexes in the PSD and play a role in the signaling mechanisms of both mGluRs and NMDA receptors . . ^^^ |
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| Interacting proteins: Q9UPX8 and P78352 |
Pubmed |
SVM Score :0.0 |
| The overexpression of the mutant of the EVH 1 domain or deletion of the extreme C terminal leucine zipper motif markedly suppressed the synaptic localization of endogenous shank but not PSD 95 or GKAP . ^^^ Actin depolymerization by latrunculin A reduced the synaptic localization of PSD Zip 45 , shank , and F actin but not of PSD 95 or GKAP . ^^^ Thus , these data suggest that the PSD Zip 45 shank and PSD 95 GKAP complexes form different synaptic compartments , and PSD Zip 45 alone or PSD Zip 45 shank is involved in the synaptic accumulation of F actin . . ^^^ |
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| Interacting proteins: Q9UPX8 and P78352 |
Pubmed |
SVM Score :0.0 |
| SAPAP 1 was not involved in the synaptic localization of PSD 95 or S SCAM , but affected that of Shank . ^^^ |
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| Interacting proteins: Q9UPX8 and P78352 |
Pubmed |
SVM Score :0.0 |
| We investigated the molecular mechanisms of Shank 1 targeting and synapse assembly by looking at the function of guanylate kinase associated protein ( GKAP ) and PSD 95 interactions . ^^^ Shank 1 when it is not associated to GKAP , which binds to the Shank PSD 95 Discs Large zona occludens 1 domain , forms filamentous and fusiform structures in which the Src homology 3 domain specifically interacts with the ankyrin repeat domain , thus allowing its multimerization via a novel form of intermolecular interaction . ^^^ Similarly , the overexpression of a GKAP mutant that is incapable of binding PSD 95 induces Shank aggregation and degradation in neurons . ^^^ Our data suggest a possible functional and structural role of the PSD 95 GKAP complex in Shank and PSD protein assembly and stability to synapses . . ^^^ |
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| Interacting proteins: Q9UPX8 and P78352 |
Pubmed |
SVM Score :0.0 |
| The splice variant of these channels expressed in medium spiny neurons contains cytoplasmic Src homology 3 and PDZ ( postsynaptic density 95 ( PSD 95 ) / Discs large / zona occludens 1 ) domains that bind the synaptic scaffolding protein Shank . ^^^ Medium spiny neurons coexpressed CaV1 . 3 interacting Shank isoforms that colocalized with PSD 95 and CaV1 . 3a channels in puncta resembling spines on which glutamatergic corticostriatal synapses are formed . ^^^ |
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| Interacting proteins: Q9UPX8 and P78352 |
Pubmed |
SVM Score :0.0 |
| At maturity we calculated that a single excitatory postsynaptic site contains 100 450 of individual postsynaptic proteins , such as PSD 95 , GKAP , Shank and Homer . ^^^ |
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| Interacting proteins: Q9UPX8 and P78352 |
Pubmed |
SVM Score :0.0 |
| These mobile clusters contain the scaffolding proteins PSD 95 , GKAP , and Shank . ^^^ |
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| Interacting proteins: Q9UPX8 and P78352 |
Pubmed |
SVM Score :0.0 |
| We show here that four major scaffolding molecules , PSD 95 , GKAP , Shank , and PSD Zip 45 , show distinct instability in total molecular content per synapse . ^^^ Indeed , acute pharmacological disruption of F actin rapidly eliminated the dynamic fraction of GKAP , Shank , and PSD Zip 45 , without changing synaptic localization of PSD 95 . ^^^ |
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| Interacting proteins: Q9UPX8 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UPX8 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UPX8 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
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