| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| The second PDZ domain in PSD 95 binds to the seven amino acid , COOH terminal domain containing the terminal tSXV motif ( where S is serine , 10 is any amino acid , and 5 is valine ) common to NR 2 subunits and certain NR 1 splice forms . ^^^ |
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| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| The activity of NMDA ( N methyl D aspartate ) receptors is mechanosensitive and dependent on the integrity of actin , suggesting a functionally important interaction between NMDA receptors and the postsynaptic cytoskeleton . alpha Actinin 2 , a member of the spectrin / dystrophin family of actin binding proteins , is identified here as a brain postsynaptic density protein that colocalizes in dendritic spines with NMDA receptors and the putative NMDA receptor clustering molecule PSD 95 . alpha Actinin 2 binds by its central rod domain to the cytoplasmic tail of both NR 1 and NR2B subunits of the NMDA receptor , and can be immunoprecipitated with NMDA receptors and PSD 95 from rat brain . ^^^ |
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| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| However , exposure of low glutamine cultures to 100 mM ethanol for 4 days ( starting at culture day 9 ) significantly increased the levels of NMDA receptor subunits ( NR 1 , NR2A , and NR2B ) and AMPA receptor subunits ( GluR 1 and GluR2 / 3 ) , but had no effect upon kainate receptor subunits ( GluR6 / 7 ) or the synapse associated proteins synapsin 1 and PSD 95 . ^^^ |
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| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| Differential interaction of the tSXV motifs of the NR 1 and NR2A NMDA receptor subunits with PSD 95 and SAP 97 . ^^^ Using the two hybrid genetic system in yeast and site directed mutagenesis , we compared the binding of the NR2A , NR 1 3 and NR 1 4 tSXV motifs with the PDZ domains of PSD 95 and SAP 97 . ^^^ |
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| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| Accordingly , NR 1 and NR2A as well as GluR 1 , GluR2 / 3 , PSD 95 and alphaCaMKII protein concentrations in post synaptic densities were the same in both control and diabetic rats , whereas the immunoreactivity for NR2B was reduced by about 40 % . ^^^ |
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| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| Expression of NR 2 receptor subunit in rat somatic sensory cortex : synaptic distribution and colocalization with NR 1 and PSD 95 . ^^^ To relate this distribution to that of NR 1 and to the NMDA receptor anchoring protein PSD 95 , we documented extensive cellular colocalization of NR2A / B with NR 1 at the light microscopic level . ^^^ Double immunolabeling revealed that NR 2 colocalized in many synapses with NR 1 and with PSD 95 . ^^^ |
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| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| In contrast , no NMDA receptor subunit NR 1 , alpha amino 3 hydroxy 5 methylisoxazole 4 propionic acid receptor subunit GluR 2 , or anchoring postsynaptic density protein 95 could be detected in rat heart at any developmental stage . ^^^ |
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| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| Immunoprecipitation experiments were conducted with an anti calnexin antibody or an anti PSD 95 antibody and the immunoprecipitates probed for NR 1 and NR 2 subunits . ^^^ Anti PSD 95 immunoprecipitates revealed an abundance of NR 1 and NR2B subunits , and these complexes were unaffected by gestational ethanol exposure . ^^^ |
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| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| Staining for the presynaptic marker synapsin 1 and the postsynaptic density proteins PSD 95 and the NR 1 subunit of NMDA receptors remained intact . ^^^ |
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| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| Double labeling experiments also were performed for PSD 95 and for the NR 1 subunit of the NMDA receptor . ^^^ |
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| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| The effect of the NR 1 knockout on intracellular localization of the NR 2 subunits was specific in that no such effect was observed for the GluR 1 and PSD 95 , two other major postsynaptic proteins . ^^^ |
|
| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| The increased levels of NR 1 and NR2B at 48 h were found associated with synaptic membranes and with increased NMDA receptor associated proteins , postsynaptic density protein 95 , neuronal nitric oxide synthase and Ca ( 2+ ) / calmodulin dependent protein kinase 2 , alpha subunit . ^^^ |
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| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| Although the lateral dendrites of mitral and tufted cells have been reported to respond to glutamate , they did not display significant plasma membrane labeling for the NR 1 subunit or for PSD 95 , suggesting that the physiological effects of glutamate at these sites are mediated by NMDA autoreceptors that are not clustered and occur only at a low density on the dendritic surface . ^^^ |
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| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| In the present study , we used in situ hybridization to measure the expression of NMDA subunits ( NR 1 , NR2A D ) , and associated intracellular proteins ( NF L , PSD 95 , and SAP 102 ) in a second , younger cohort from the Stanley Foundation Neuropathology Consortium , which included patients with both schizophrenia and affective disorders . ^^^ |
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| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| Furthermore , CK 2 colocalizes with NR 1 and PSD 95 at synaptic sites . ^^^ |
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| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| Further characterization of the molecular interaction between PSD 95 and NMDA receptors : the effect of the NR 1 splice variant and evidence for modulation of channel gating . ^^^ Coexpression of PSD 95 ( c Myc ) with NR 1 1a / NR2A NMDA receptors in human embryonic kidney ( HEK ) 293 cells resulted in a decrease in efficacy for the glycine stimulation of [ 3 H ] MK 801 binding similar to that previously described for l glutamate . ^^^ The inhibition constants ( K ( 1 ) s ) for the binding of l glutamate and glycine to NR 1 1a / NR2A determined by [ 3 H ] CGP 39653 and [ 3 H ] MDL 105 519 displacement assays , respectively , were not significantly different between NR 1 1a / NR2A receptors coexpressed + / PSD 95 ( c Myc ) . ^^^ Total NR 1 4b and NR 1 4b / NR2A expression was enhanced by PSD 95 ( c Myc ) but whole cell enzyme linked immunoadsorbent assays ( ELISAs ) showed that this increase was not due to increased expression at the cell surface . ^^^ |
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| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| Immunoblot analyses of cell homogenates indicated upregulation of NMDA receptor subunits ( NR 1 , NR2A , and NR2B ) , but downregulation of synaptic organizing proteins ( PSD 95 , densin 180 , and septin 6 homologue ) and a synapse enriched enzyme ( alphaCaMKII ) . ^^^ |
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| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| In addition , changes in the serine phosphorylation of NR 1 and in the expression of postsynaptic density protein , PSD 95 , were measured as potential molecular mechanisms for the cocaine induced alterations in receptor subunit expression . ^^^ |
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| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| The effect of environmental enrichment on NR 1 and BDNF gene expression was specific to Pb2+ exposed animals and was present in the absence of changes in the NR2B subunit of the N methyl D aspartate receptor , GluR 1 , alpha CamKII , or PSD 95 gene expression measured in the same animals . ^^^ |
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| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| Hippocampal mRNA expression of the NMDA subunits NR 1 , NR2A and NR2B , insulin like growth factor type 1 receptor ( IGF 1R ) , and postsynaptic density protein 95 ( PSD 95 ) was then measured in the animals by Northern blot analysis . ^^^ |
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| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| In the present study , we performed western blot analysis to determine whether protein levels of NMDA receptor subunits ( NR 1 , NR2A , NR2B ) and associated PSD proteins ( NF L , PSD 95 , SAP 102 ) are altered in schizophrenia . ^^^ |
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| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| Applying semi quantitative immunoblotting , we found that in the whole tetanized hippocampus , synaptic expression of the N methyl D aspartate and alpha amino 3 hydroxyl 5 methyl 4 isoxazole propionate receptor subunits ( NR 1 , NR2A , glutamate receptor 1 ) and their associated partners , e . g . synaptic associated protein 97 , postsynaptic density protein 95 , alpha subunit of Ca2+ / calmodulin dependent protein kinase 2 , neuronal nitricoxide synthase , increased 180 min post high frequency stimulation . ^^^ |
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| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| Myosin RLC co localized with NR 1 in the dendritic spines of isolated hippocampal neurons , and was co immunoprecipitated from brain extracts in a complex with NR 1 , NR2A , NR2B , PSD 95 , Adaptor protein 2 and myosin 2 heavy chain . ^^^ |
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| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| Using immunoautoradiography the expression levels of NR 1 and NR2B subunits of the NMDA receptor and the postsynaptic density scaffold proteins , PSD 95 , PSD 93 , and neurofilament light ( NFL ) were examined in normal common marmosets ( Callithrix jacchus ) and 1 methyl 4 phenyl 1 , 2 , 3 , 6 tetrahydropyridine ( MPTP ) lesioned animals that exhibited high or low levels of l DOPA induced dyskinesia . ^^^ |
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| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| NMDA receptor subunit NRI and postsynaptic protein PSD 95 in hippocampus and orbitofrontal cortex in schizophrenia and mood disorder . ^^^ We have used immunoautoradiographical techniques to assess the expression of the obligatory NMDA receptor subunit NR 1 and an associated post synaptic density protein PSD 95 in the hippocampal dentate gyrus and orbitofrontal cortex ( OFC ) in schizophrenia and mood disorder . ^^^ Optical density measures from film autoradiographs revealed no changes in NR 1 or PSD 95 in the OFC or dentate hilus , however a decrease in PSD 95 was found in the dentate molecular layer in both schizophrenia and bipolar disorder relative to major depression . ^^^ |
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| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| In these rats , however , another drug challenge produced a rapid reduction in PSD 95 immunoreactivity and prevented the learning specific increase in the NMDA receptor NR 1 subunit and phosphorylated CaMKII . ^^^ |
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| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| The close apposition of the presynatic marker synaptophysin to these long spines and the presence of specific postsynaptic markers actin , PSD 95 , NR 1 , and GluR 1 suggested the existence of functional excitatory synaptic contacts . ^^^ |
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| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the association between PSD 95 and NMDAR subunits ( NR 1 , NR2A , and NR2B ) in the hippocampal CA 1 was also markedly altered by perinatal hypoxia . ^^^ |
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| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UHB4 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|