| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| Here , we describe the isolation of a novel Ras GTPase activating protein , SynGAP , that interacts with the PDZ domains of PSD 95 and SAP 102 in vitro and in vivo . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| Postsynaptic localization of N methyl D aspartate type glutamate receptors may be mediated by the synapse associated proteins ( SAPs ) SAP 90 , SAP 102 , and chapsyn 110 . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| SAP 90 and SAP 102 coimmunoprecipitate with both KA 2 and GluR 6 , but only SAP 97 coimmunoprecipitates with GluR 6 . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| Double labeling experiments were performed for PSD 95 and for SAP 102 or PSD 93 , respectively , two other members of the family of synapse associated proteins . ^^^ In the OPL , however , PSD 95 and PSD 93 were found presynaptically , whereas SAP 102 was located postsynaptically at photoreceptor synapses . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| PSD 95 ( SAP 90 ) , SAP 102 and Chapsyn 110 ( PSD 93 ) are members of the membrane associated guanylate kinase family , and interact with N methyl D aspartate ( NMDA ) receptor NR2A ( GluRepsilon 1 ) and NR2B ( GluRepsilon 2 ) subunits and with Shaker type K+ channel subunits to cluster into a channel complex . ^^^ PSD 95 and SAP 102 mRNAs were prominently expressed at embryonic day 13 ( E 13 ) in the mantle zone of various brain regions , where NMDA receptor NR2B subunit mRNA is expressed at high levels . ^^^ PSD 95 ( SAP 90 ) , SAP 102 and Chapsyn 110 ( PSD 93 ) are members of the membrane associated guanylate kinase family , and interact with N methyl D aspartate ( NMDA ) receptor NR2A ( GluRepsilon 1 ) and NR2B ( GluRepsilon 2 ) subunits and with Shaker type K+ channel subunits to cluster into a channel complex . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| Overexpression of cypin in hippocampal neurons specifically perturbs postsynaptic trafficking of PSD 95 and SAP 102 , an effect not produced by overexpression of other PDZ ligands . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| The membrane associated guanylate kinases [ Chapsyn 110 / postsynaptic density 93 ( PSD 93 ) , synapse associated protein 90 ( SAP 90 ) / PSD 95 , and SAP 102 ] are believed to cluster and anchor NMDA receptors at the synapse and to play a role in signal transduction . ^^^ At postnatal day 2 ( P 2 ) , SAP 102 was highly expressed , whereas PSD 93 and PSD 95 were low . ^^^ From Western blots of hippocampus and immunogold analysis of CA 1 synapses , the high expression of NR2B at P 2 coincides with the high level of SAP 102 at synapses , whereas the later expression of NR2A coincides with that of PSD 93 and PSD 95 . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| Using the yeast two hybrid system , we found that the C terminal region of ErbB 4 interacts with the three related membrane associated guanylate kinases ( MAGUKs ) PSD 95 / SAP90 , PSD 93 / chapsyn 110 , and SAP 102 , which harbor three PDZ domains , as well as with beta ( 2 ) syntrophin , which has a single PDZ domain . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| The majority of NMDA receptor clusters were colocalized with the postsynaptic density proteins PSD 95 , PSD 93 , and SAP 102 . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| Whereas PSD 95 and PSD 93 occur only at postsynaptic sites in hippocampal neurons , SAP 102 also occurs in axons . ^^^ In heterologous cells , PSD 95 and PSD 93 mediate cell surface ion channel clustering , but SAP 102 and SAP 97 do not . ^^^ This selective ion channel clustering activity by MAGUKs is explained by differential palmitoylation , as PSD 93 and PSD 95 are palmitoylated though SAP 97 , and SAP 102 are not . ^^^ And , appending the N terminus of SAP 102 to PSD 95 results in localization of the chimera to both axons and dendrites . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| The amino acid sequences deduced indicate that A . leptorhynchus neurons express homologues of the mammalian PSD 93 , SAP 97 , and SAP 102 MAGUKs and two homologues of mammalian PSD 95 . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| Postsynaptic density ( PSD ) 95 , SAP 102 , and Chapsyn 110 are members of the PSD 95 / SAP90 protein family , which interact with the C terminus of N methyl D aspartate ( NMDA ) receptor and shaker type potassium channel subunits . ^^^ Second , the resulting overall distributions of PSD 95 , SAP 102 , and Chapsyn 110 in the adult mouse brain were consistent with their mRNA distributions . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| Similarly , when the N terminus of PSD 95 is replaced with the non palmitoylated N terminus of SAP 102 , postsynaptic targeting is maintained . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| We quantitatively defined the contributions of carboxyl terminal residues to binding selectivity of the prototypic interactions of the PDZ domains of postsynaptic density protein 95 ( PSD 95 ) and its homolog synapse associated protein 90 ( SAP 102 ) with the NR2b subunit of the N methyl d aspartate type glutamate receptor . ^^^ This analysis predicts a COOH terminal ( E / Q ) ( S / T ) 15 consensus sequence for the strongest binding to the first two PDZ domains of PSD 95 and SAP 102 . ^^^ A search of the human genome sequences for proteins with a COOH terminal ( E / Q ) ( S / T ) 15 motif yielded 50 proteins , many of which have not been previously identified as PSD 95 or SAP 102 binding partners . ^^^ Two of these proteins , brain specific angiogenesis inhibitor 1 and protein kinase Calpha , co immunoprecipitated with PSD 95 and SAP 102 from rat brain extracts . . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| The C terminus of GluR A bound to the PDZ domains of SAP 97 , but not to those of three related MAGUKs , PSD 93 , PSD 95 , and SAP 102 . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| ErbB 4 associates with the PSD 95 ( also known as SAP 90 ) family members including PSD 95 , SAP 97 , and SAP 102 whereas ErbB 2 interacts with Erbin and PICK 1 . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| METHODS AND RESULTS : SAP 97 , PSD 95 , Chapsyn and SAP 102 messengers were detected by reverse transcriptase polymerase chain reaction ( RT PCR ) on mRNA extracted from both whole myocardium and isolated myocytes . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| Interaction of the tyrosine kinase Pyk 2 with the N methyl D aspartate receptor complex via the Src homology 3 domains of PSD 95 and SAP 102 . ^^^ This interaction is mediated by PSD 95 and its homolog SAP 102 . ^^^ The proline rich regions in the C terminal half of Pyk 2 bind to the SH 3 domain of PSD 95 and SAP 102 . ^^^ The SH 3 and guanylate kinase homology ( GK ) domain of PSD 95 and SAP 102 interact intramolecularly , but the physiological significance of this interaction has been unclear . ^^^ Characterization of PSD 95 and SAP 102 as adaptor proteins for Pyk 2 fills a critical gap in the understanding of the spatial organization of the Pyk 2 Src signaling pathway at the postsynaptic site and reveals a physiological function of the intramolecular SH 3 GK domain interaction in SAP102 . . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| The PSD 95 like membrane associated guanylate kinase ( ' MAGUK ' ) family contains three additional members : PSD 93 , synapse associated protein 97 ( SAP 97 ) and SAP 102 . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| METHOD : Using in situ hybridization , the authors examined expression of the transcripts encoding NR ( 1 ) isoforms containing exons 5 , 21 , or 22 , and the NMDA receptor related postsynaptic density proteins NF L , PSD 93 , PSD 95 , and SAP 102 . ^^^ Increased expression of the NMDA receptor associated postsynaptic density proteins NF L , PSD 95 , and SAP 102 was also detected in the thalamus of subjects with schizophrenia . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| In addition , we show that PSD 95 is unique among PSD 95 family members in that the other members , PSD 93 , SAP 97 , and SAP 102 , do not physically associate with Lyn , Src , or Yes . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| These PDZ proteins include the four neuronal membrane associated guanylate kinases , PSD 95 / SAP 90 , PSD 93 / Chapsyn 110 , SAP 97 / hDLG and SAP 102 ; the multi PDZ protein , MUPP 1 ; and the mitochondrial PDZ protein , OMP 25 . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| Each family of PSD proteins is selective for a given glutamate receptor subtype , the most well characterized being the NMDA receptor binding proteins PSD 93 , PSD 95 , NF L , and SAP 102 . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| The MAGUK family comprises the post synaptic density 95 ( PSD 95 ) protein and closely related molecules such as chapsyn 110 , synapse associated protein 102 ( SAP 102 ) , and SAP 97 . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| In this study , we investigated dopamine glutamate interactions by measuring the expression of transcripts encoding the subunits for the ionotropic glutamate receptors ( NMDA , AMPA and kainate ) and five NMDAR associated intracellular proteins , PSD 93 , PSD 95 , SAP 102 , NF L and yotiao in the dopaminergic neurons in the substantia nigra pars compacta ( SNc ) of subjects with schizophrenia and a comparison group . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| In the present study , we used in situ hybridization to measure the expression of NMDA subunits ( NR 1 , NR2A D ) , and associated intracellular proteins ( NF L , PSD 95 , and SAP 102 ) in a second , younger cohort from the Stanley Foundation Neuropathology Consortium , which included patients with both schizophrenia and affective disorders . ^^^ In particular , we found decreased NF L , PSD 95 , and SAP 102 transcripts in bipolar disorder , and decreased SAP 102 levels in major depression . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| We review the potential roles in brain plasticity of two membrane associated guanylate kinases ( MAGUKs ) , SAP 102 and PSD 95 , which form a scaffold for the ion passing glutamate receptors at the postsynaptic density , and we consider the known functional significance of these molecules in subunit switching . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| Presently , only indirect evidence of changes in PMCA protein expression during postnatal development exists , therefore we chose to examine the postnatal developmental protein expression patterns of PMCAs 1 4 and the SAP proteins SAP 102 and PSD 95 . ^^^ The results of this study demonstrate that postnatal expression of all PMCAs , SAP 102 and PSD 95 is similar in both the in vivo hippocampus and the in vitro organotypic hippocampal slice culture . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| Neuroligin binds to the PDZ domain containing proteins PSD 95 , SAP 102 , Chapsyn 110 , S SCAM , Magi 1 and 3 , Shank 1 and 3 , Pick 1 , GOPC , SPAR , Semcap 3 and PDZ RGS 3 . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of Ser 1480 disrupts the interaction of NR2B with the PDZ domains of PSD 95 and SAP 102 and decreases surface NR2B expression in neurons . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : Drosophila discs large ( DLG ) is the sole representative of a large class of mammalian MAGUKs , including human DLG , SAP 97 , SAP 102 , and PSD 95 . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| Lipid rafts accounted for the majority of PSD 95 , whereas SAP 102 was predominantly located in PSDs . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| Development was marked by substantial decreases in NR2B and SAP 102 and increases in NR2A , PSD 95 , AMPA receptors , and CaMKII . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| We found decreased striatal expression of transcripts encoding PSD 95 and SAP 102 in bipolar disorder and of SAP 102 in major depression and schizophrenia , while no significant changes in NF L and PSD 93 mRNAs were observed . ^^^ Abnormal expression of SAP 102 in schizophrenia and SAP 102 and PSD 95 in mood disorders in subcortical structures receiving afferent glutamatergic innervation from frontal cortex suggests dysregulation of cortical subcortical circuitry in these illnesses . . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| Pins modulates PSD 95 and SAP 102 trafficking and influences NMDA receptor surface expression . ^^^ We show that mPins ( mammalian homologue of Drosophila melanogaster partner of inscuteable ) interacts with SAP 102 and PSD 95 ( two PDZ proteins present in neurons ) , and functions in the formation of the NMDAR MAGUK ( N methyl D aspartate receptor membrane associated guanylate kinase ) complex . mPins enhances trafficking of SAP 102 and NMDARs to the plasma membrane in neurons . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| SALM 1 interacts with PSD 95 , synapse associated protein 102 ( SAP 102 ) , and SAP 97 based on coimmunoprecipitation of detergent solubilized brain . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| Postsynaptic expression of synapse associated protein 97 ( SAP 97 ) increased presynaptic protein content and active zone size to a greater extent than comparable amounts of postsynaptic PSD 95 ( postsynaptic density 95 ) or SAP 102 . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| These changes in spine density were accompanied by altered expression of proteins known to interact with PSD 95 , including NR2B and SAP 102 , suggesting that PSD 95 plays a role in regulating the expression and activation of proteins found within the NMDA receptor complex . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| In addition , we studied expression of the NMDA interacting PSD molecules NF L , SAP 102 , PSD 95 and PSD 93 in ACC and DLPFC at both transcriptional and translational levels . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| In the present study , we performed western blot analysis to determine whether protein levels of NMDA receptor subunits ( NR 1 , NR2A , NR2B ) and associated PSD proteins ( NF L , PSD 95 , SAP 102 ) are altered in schizophrenia . ^^^ |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92796 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
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