| Interacting proteins: P78314 and P62993 |
Pubmed |
SVM Score :0.0 |
| Specific motifs recognized by the SH 2 domains of Csk , 3BP2 , fps / fes , GRB 2 , HCP , SHC , Syk , and Vav . ^^^ We report here the optimal recognition motifs for SH 2 domains from GRB 2 , Drk , Csk , Vav , fps / fes , SHC , Syk ( carboxy terminal SH 2 ) , 3BP2 , and HCP ( amino terminal SH 2 domain , also called PTP1C and SHPTP 1 ) . ^^^ As predicted , SH 2 domains from proteins that fall into group 1 on the basis of a Phe or Tyr at the beta D 5 position ( GRB 2 , 3BP2 , Csk , fps / fes , Syk C terminal SH 2 ) select phosphopeptides with the general motif phospho Tyr hydrophilic ( residue ) hydrophilic ( residue ) hydrophobic ( residue ) . ^^^ |
|
| Interacting proteins: P78314 and P62993 |
Pubmed |
SVM Score :0.0 |
| In addition to Zap 70 , the 3BP2 SH 2 domain associated in vitro with LAT , Grb 2 , PLCgamma 1 , and Cbl from activated T cell lysates . ^^^ |
|
| Interacting proteins: P78314 and P62993 |
Pubmed |
SVM Score :0.0 |
| Functional studies demonstrated that CR 2 activation induced , within a brief span of 2 min , tyrosine phosphorylation of nucleolin and its interaction with Src homology 2 domains of the p 85 subunit of PI 3 kinase and of 3BP2 and Grb 2 , but not with Src homology 2 domains of Fyn and Gap . ^^^ |
|
| Interacting proteins: P78314 and P62993 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of LAT creates binding sites for the Src homology 2 ( SH 2 ) domains of other proteins , including PLC gamma 1 , Grb 2 , Gads , Grap , 3BP2 , and Shb , and indirectly binds SOS , c Cbl , Vav , SLP 76 , and Itk . ^^^ |
|
| Interacting proteins: P78314 and P62993 |
Pubmed |
SVM Score :0.0 |
| To further define the events linking the membrane distal tyrosine and JNK activation , the Src homology 2 domains of Shc , Grb 2 , and 3BP2 were shown to bind the full length GCSF R and a phosphopeptide encompassing the membrane distal tyrosine . ^^^ When binding to variant phosphopeptides based on this membrane distal tyrosine was tested , altering the amino acids immediately following the phosphotyrosine could selectively abolish the interaction with Shc or Grb 2 , or the binding to both Grb 2 and 3BP2 . ^^^ When these changes were introduced into the full length GCSF R and new cell lines created , only the mutant that did not interact with Grb 2 and 3BP2 did not activate JNK . ^^^ |
|