| Interacting proteins: P60709 and P68104 |
Pubmed |
SVM Score :0.0 |
| Compared to those in adjacent normal epitheliums , the expression of 15 proteins including enolase , elongation factor Tu , isocitrate dehydrogenase , tubulin alpha 1 chain , tubulin beta 5 chain , actin ( cytoplasmic 1 ) , glyceraldehyde 3 phosphate dehydrogenase , tropomyosin isoform 4 ( TPM 4 ) , prohibitin , peroxiredoxin 1 ( PRX 1 ) , manganese containing superoxide dismutase ( MnSOD ) , neuronal protein , and transgelin was up regulated ; and the expression of five proteins including TPM 1 , squamous cell carcinoma antigen 1 ( SCCA 1 ) , stratifin , peroxiredoxin 2 isoform a , and alpha B crystalline was down regulated in cancer tissues with a statistical significance ( p < 0 . 05 ) . ^^^ |
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| Interacting proteins: P60709 and P68104 |
Pubmed |
SVM Score :0.0 |
| Northern blot analysis of various non neoplastic and neoplastic cultured cells of different stages of confluence , cell shape , and cell cycle status revealed that EF 1 alpha had a lower and more homogeneous expression than did beta actin . ^^^ |
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| Interacting proteins: P60709 and P68104 |
Pubmed |
SVM Score :0.0 |
| Other regulatory mechanisms contribute to the elevated expression of the transcripts in rho 0 cells , as suggested by cycloheximide enhancement of the accumulation of the mRNAs for EF 1 alpha and beta actin in rho 0 cells , but not in parental rho+ cells . ^^^ |
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| Interacting proteins: P60709 and P68104 |
Pubmed |
SVM Score :0.0 |
| As one of the hypotheses for the pathogenesis of brain damage in DS is oxidative stress and cells of patients with DS are more susceptible to ionizing irradiation , we decided to study ERCC 2 , ERCC 3 and XRCC 1 , representatives of repair genes known to be involved in the repair of oxidative DNA damage . mRNA steady state levels of ERCC 2 , ERCC 3 , XRCC 1 , a transcription activator ( TAF DBP ) and an elongation factor ( EF1A ) were determined and normalized versus the housekeeping gene beta actin in five individual brain regions of nine controls and nine DS patients . ^^^ |
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| Interacting proteins: P60709 and P68104 |
Pubmed |
SVM Score :0.0 |
| GFP expression was driven by a variety of strong viral and cellular promoters , including the murine stem cell virus ( MSCV ) long terminal repeat ( LTR ) , a Gibbon ape leukemia virus ( GALV ) LTR , the human elongation factor 1alpha ( EF1alpha ) promoter , the composite CAG promoter ( consisting of the CMV immediate early enhancer and the chicken beta actin promoter ) , and the human phosphoglycerate kinase 1 ( PGK ) promoter . ^^^ |
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| Interacting proteins: P60709 and P68104 |
Pubmed |
SVM Score :0.0 |
| We demonstrate in this work that EF1alpha colocalizes with beta actin mRNA and F actin in protrusions of chicken embryo fibroblasts and binds directly to F actin and beta actin mRNA simultaneously in vitro in actin cosedimentation and enzyme linked immunosorbent assays . ^^^ We propose that the EF1alpha F actin complex is the scaffold that is important for beta actin mRNA anchoring . ^^^ We demonstrate that either domain 3 of EF1alpha or the EF1alpha binding site of Bni1p inhibits EF1alpha binding to beta actin mRNA in vitro and causes delocalization of beta actin mRNA in chicken embryo fibroblasts . ^^^ Taken together , these results implicate EF1alpha in the anchoring of beta actin mRNA to the protrusion in crawling cells . . ^^^ |
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| Interacting proteins: P60709 and P68104 |
Pubmed |
SVM Score :0.0 |
| To control for the variation in sample processing and in reverse transcription reaction among samples , shrimp beta actin and elongation factor 1alpha ( EF 1alpha ) genes were amplified in parallel with the viral cDNA . ^^^ The sensitivity and the efficiency of amplification of EF 1alpha was greater than beta actin when compared to TSV and YHV amplification efficiency suggesting that EF 1alpha is a better internal control for the RT PCR detection of TSV and YHV . ^^^ In addition , sample to sample variation in EF 1alpha C ( T ) value was lower than the variation in beta actin C ( T ) value of the corresponding samples . ^^^ The specificity of TSV , YHV , EF 1alpha and beta actin amplifications was confirmed by analyzing the dissociation curves of the target amplicon . ^^^ |
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| Interacting proteins: P60709 and P68104 |
Pubmed |
SVM Score :0.0 |
| Of the six types of promoters tested , significantly higher transduction efficiencies were achieved with the human elongation factor 1alpha promoter ( EF1alpha promoter ) , the human cytomegalovirus ( CMV ) immediate early 1 gene enhancer / beta actin promoter with beta actin intron ( CA promoter ) , and the CMV promoter / enhancer with the largest intron of CMV ( intron A ) ( CMVi promoter ) in the human CD34+ cells and the immature subsets ( CD34+ CD 38 ( low / ) and CD34+ AC133+ subsets ) . ^^^ |
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| Interacting proteins: P60709 and P68104 |
Pubmed |
SVM Score :0.0 |
| We prepared four LacZ expressing Ad vectors , each of which contained one of the following : Rous sarcoma virus ( RSV ) , cytomegalovirus ( CMV ) , beta actin promoter / CMV enhancer ( CA ) , or EF 1alpha promoter . ^^^ |
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| Interacting proteins: P60709 and P68104 |
Pubmed |
SVM Score :0.0 |
| The expression levels of three commonly used housekeeping genes , EF 1 alpha , RPS 20 and Beta Actin , were examined in seven different tissues and leucocytes from non stimulated Atlantic salmon ( Salmo salar L . ) . ^^^ Stimulation with lipopolysaccharide ( LPS ) of TO cells and HKL from Atlantic salmon was additionally performed to reveal whether an immune stimulating agent would change the expression level of EF 1 alpha , RPS 20 and Beta Actin . ^^^ LPS stimulation of cells revealed that RPS 20 and EF 1 alpha were least regulated by the LPS treatment in the TO cells relative to 18S rRNA , but in HKL , Beta Actin was the most appropriate gene . ^^^ |
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| Interacting proteins: P60709 and P68104 |
Pubmed |
SVM Score :0.0 |
| Analysis of qRT PCR data using the geNorm software indicated that , although commonly used as internal controls , beta actin ( ACT ) and translation elongation factor 1alpha ( eEF1A ) might not be the best choice due to variable expression across different life stages of P . parasitica . ^^^ |
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| Interacting proteins: P60709 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P60709 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
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