| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.85250672 |
| In eukaryotes , the eukaryotic translation elongation factor eEF1A responsible for transporting amino acylated tRNA to the ribosome forms a higher order complex , eEF1H , with its guanine nucleotide exchange factor eEF1B . 0.85250672^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| Effect of EF 1b on EF 1a and ribosome dependent GTPase activity . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| Southern analysis indicates that EF 1 beta in the human genome , like EF 1 alpha , appears to be specified by more than one gene . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| It was also shown that a stoichiometric complex was formed between EF 1 alpha and EF 1 beta gamma from S . pombe . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| EF 1 beta gamma and of EF 1 beta gamma to EF 1 alpha . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| EF 1H is readily converted to EF 1 alpha and EF 1 beta gamma on anion exchange columns . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| The EF 1 alpha activity was enhanced by rabbit EF 1 beta gamma . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| We propose that the guanine nucleotide exchange stimulation by EF 1 beta is induced through binding of this factor to the carboxy terminal part of EF 1 alpha . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| In contrast to amino terminal fragments , those derived from the carboxy terminal part of EF 1 beta were still active in enhancing the guanine nucleotide exchange of GDP bound to EF 1 alpha . ^^^ We conclude that the amino terminal halves of both EF 1 beta and EF 1 gamma are firmly attached to each other , and that the carboxy terminal part of EF 1 beta interacts with EF 1 alpha . . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| EF 1 beta catalyzes the exchange of guanine nucleotides bound to EF 1 alpha ; the latter protein is the eukaryotic equivalent of bacterial EF Tu . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| EF 1 is composed of a nucleotide binding protein EF 1 alpha , and a nucleotide exchange protein complex , EF 1 beta gamma , while EF 2 catalyses the translocation of peptidyl tRNA on the ribosome . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| Extensively purified EF 1H ( EF 1 alpha beta beta ' gamma ) from wheat embryos had a protein kinase activity and phosphorylated EF 1 beta which is one of the two EF Ts like factors ( EF 1 beta and EF 1 beta ' ) . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| Both EF 1 beta gamma and EF 1 beta have been shown to stimulate the following three reactions to a comparable degree : ( a ) exchange of GDP bound to EF 1 alpha with exogenous GDP ; ( b ) EF 1 alpha dependent binding of Phe tRNA to ribosomes ; ( c ) poly ( U ) dependent poly ( phenylalanine ) synthesis . ^^^ It is remarkably that about 5 % of the cellular pool of EF 1 beta gamma was found to be present in membrane fractions , under conditions where no EF 1 alpha was detectable in these fractions . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| The rate of nucleotide exchange on EF 1 alpha as catalyzed by EF 1 beta was found to be affected reversibly by the state of phosphorylation of EF 1 beta . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| EF 1 beta , the factor enhancing the EF 1 alpha GDP / GTP exchange , is part of EF 1H and of smaller aggregates . ^^^ The fraction of EF 1 alpha and EF 1 beta not associated with EF 1H , and EF 2 have been purified to homogeneity after several chromatographic steps . ^^^ EF 1H consists of many proteins ; among them , EF 1 alpha , EF 1 beta and an EF 1 gamma like protein represent three of the major components . ^^^ The relative molecular mass of the purified factors have been determined as : EF 1 alpha , 50 , 000 ; EF 1 beta , 30 , 000 ; the EF 1 gamma like component , 49 , 000 ; EF 2 , 85 , 000 . ^^^ Some cross reactivity with the antibodies against the prokaryotic counterparts has been shown for EF 1 alpha , EF 1 beta and EF 2 by functional and immuno precipitation methods , suggesting the existence of structural homologies . . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| EF 1 beta strongly enhances the dissociation rate of the EF 1 alpha 10 GDP complex and to a lesser extent of the EF 1 alpha 10 GTP complex . ^^^ Although in this regard the effect of kirromycin mimics that of EF 1 beta , the antibiotic is incapable of increasing the EF 1 alpha 10 GDP / GTP exchange rate when aa tRNA and ribosomes are present . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| Using the conventional methods of gel filtration and ion exchange chromatography , various forms of elongation factor 1 ( EF 1 alpha , EF 1 beta delta , EF 1 beta gamma delta ) have been purified from rabbit reticulocyte lysate . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| Purification and characterization of three elongation factors , EF 1 alpha , EF 1 beta gamma , and EF 2 , from wheat germ . ^^^ Three elongation factors , EF 1 alpha , EF 1 beta gamma and EF 2 , have been isolated from wheat germ . ^^^ EF 1 beta gamma decreases the amount of EF 1 alpha required for polyphenylalanine synthesis about 20 fold . ^^^ EF 1 beta gamma enhances the ability to EF 1 alpha to support the binding of Phe tRNA to the ribosomes and enhances the GTPase activity of EF 1 alpha . ^^^ Wheat germ EF 1 alpha , EF 1 beta gamma , and EF 2 support polyphenylalanine synthesis on rabbit reticulocyte ribosomes as well as on yeast ribosomes . . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| The activity of EF 1 alpha is enhanced in polyphenylalanine synthesis by a complementary component , EF 1 beta delta . ^^^ It is also shown that EF 1 beta delta can facilitate an exchange of EF 1 alpha bound GDP for GTP . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| In contrast to high molecular weight forms of elongation factor 1 ( EF 1H ) from animal sources which contain three subunits , EF 1a , EF 1b , and EF 1c , EF 1H from wheat embryo consisted of four subunits , EF 1a , EF 1b , EF 1b ' , and EF 1c , in an equimolar ratio . ^^^ The molecular weights of EF 1a , EF 1b , EF 1b ' , and EF 1c from wheat embryo were 52 , 000 , 29 , 000 , 28 , 000 , and 48 , 000 , respectively . ^^^ In the animal system , EF 1a and EF 1b correspond functionally to EF Tu and EF Ts , respectively . ^^^ In the wheat system , however , both EF 1b and EF 1b ' had the EF Ts like activity to stimulate EF 1a dependent binding of aminoacyl tRNA to ribosomes . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| Dissociation of highly purified EF 1 alpha beta gamma ( a high molecular weight form of polypeptide chain elongation factor 1 ) from pig liver into EF 1 alpha and EF 1 beta gamma at various temperatures was examined and the following results were obtained . ( 1 ) When dissociation of EF 1 alpha beta gamma was analyzed by gel filtration with Sephacryl S 200 , it was found that in the absence of GTP , it did not dissociate at any temperature between 4 and 37 degrees C , whereas in the presence of GTP , it tended to dissociate with elevation of the temperature , and almost complete dissociation was observed at 32 degrees C . ^^^ This indicated that the dissociation constant of EF 1 alpha beta gamma into EF 1 alpha and EF 1 beta gamma in the presence of GTP increased with increase in the temperature . ( 2 ) When gel filtration was performed in the presence of both GTP and [ 14C ] Phe tRNA , the formation of a ternary complex of EF 1 alpha . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| The high molecular weight form of polypeptide chain elongation factor 1 , or EF 1H , has been purified from pig liver to apparent homogeneity and shown to be a 1 : 1 : 1 complex of EF 1 alpha , EF 1 beta , and EF 1 gamma , namely , EF 1 alpha beta gamma ( Hattori , S . and Iwasaki , K . ( 1980 ) J . ^^^ Furthermore , it was reported that EF 1 alpha beta gamma seemed to dissociate into EF 1 alpha and EF 1 beta gamma when guanine nucleotides were present , and also to form a complex of EF 1 alpha . ^^^ The controversial results obtained by different methods as described above seemed to be compatible with each other , if one assumes that the dissociation constant for the conversion of EF 1 alpha beta gamma into EF 1 alpha and EF 1 beta gamma increased with the increase in temperature . ^^^ GTP + EF 1 beta gamma EF 1 alpha . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| Three forms of elongation factor 1 ( EF1A , EF1B and EF1C ) were isolated from wheat germ ; the preparation obtained by successive chromatography on DEAE Sephadex A 50 , hydroxylapatite and Sephadex G 200 showed about 90 % purity . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| Expression of elongation factor 1 alpha ( EF 1 alpha ) and 1 beta gamma ( EF 1 beta gamma ) are uncoupled in early Xenopus embryos . ^^^ The functional recycling of EF 1 alpha ( conversion of EF 1 alpha GDP to EF 1 alpha GTP ) is assured by the EF 1 beta gamma complex . ^^^ We show here that in Xenopus laevis embryos , contrary to the situation for EF 1 alpha , EF 1 beta , and EF 1 gamma mRNAs are transcribed from the same genes in oocytes and somatic cells . ^^^ In addition , the onset of transcription of the EF 1 beta and EF 1 gamma genes from the zygotic genome occurs several hours after that of the somatic EF 1 alpha S gene . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| EF 1C is a component of the aggregate EF 1B , consisting of the subunit forms EF 1A . ^^^ The subunit form EF 1C stimulates binding of aminoacyl tRNA to ribosomes , catalysed by EF 1A , similarly as EF 1 beta gamma which stimulates the activity of EF 1 in other eukaryotic cells . ^^^ Thermal sensitivity of EF 1A was much higher than that of EF 1B , thus a protective role of EF 1C in the EF 1A . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| The heterogeneous elongation factor eEF 1 , isolated from Guerin epithelioma , can be separated into three fractions : one of them functionally corresponds to EF 1 alpha , the second on to EF 1 beta gamma , and the third is an unidentified , active aggregate named EF 1B , which contains the subunit forms EF 1 alpha and EF 1 beta gamma , and other polypeptides showing protein kinase activity . ^^^ The aggregate EF 1B can be autophosphorylated , while the subunit forms EF 1 alpha and EF 1 beta gamma can neither become autophosphorylated nor phosphorylate other polypeptides . ^^^ EF 1 beta gamma is thermostable and protects against thermal inactivation of EF 1 alpha in the EF 1 alpha EF 1 beta gamma complex . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| The eukaryotic elongation factor 1 ( EF 1 ) consists of four subunits , EF 1 alpha , EF 1 beta , EF 1 gamma and EF 1 delta which induce efficient transfer of aminoacyl tRNA to the ribosome . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| Polyclonal antibodies directed against the two components of EF 1 , the G protein EF 1 alpha and the guanine nucleotide exchange complex EF 1 beta gamma delta , were used for the analysis of EF 1 . ^^^ We show that Xenopus oocytes as well as Xenopus A 6 cultured cells contain comparable ratios around 2 : 1 of EF 1 alpha versus EF 1 beta gamma delta . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| The present article is a review of the work done on the elongation factors EF 1 alpha , EF 2 and EF 1 beta isolated from the hyperthermophilic archaeon Sulfolobus solfataricus . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| EF 1 beta beta ' gamma catalyzes the exchange of GDP for GTP on EF 1 alpha and stimulates the binding of EF 1 alpha dependent aminoacyl tRNA to ribosomes . ^^^ We examined the region of EF 1 beta ' that binds to EF 1 alpha by in vitro binding assays , and examined the GDP / GTP exchange activity using deletion mutants of a GST EF 1 beta ' fusion protein . ^^^ We thereby suggested a pivotal amino acid region , residues 189 222 , of EF 1 beta ' for binding to EF 1 alpha . . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| Crystals of the Saccharomyces cerevisiae elongation factor eEF1A ( formerly EF 1 alpha ) in complex with a catalytic C terminal fragment of the nucleotide exchange factor eEF1B alpha ( formerly EF 1 beta ) were grown by the sitting drop vapour diffusion technique , using polyethylene glycol 2000 monomethyl ether as precipitant . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| One of the eukaryotic polypeptide chain elongation factors , EF 1 beta gamma delta complex , is involved in polypeptide chain elongation via the GDP / GTP exchange activity of EF 1 alpha . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| Genomic and cDNA clones coding for elongation factor 1 beta ( eEF 1 beta ) from Arabidopsis thaliana ( At ) were isolated and characterized . eEF 1 beta was found to be encoded by a single copy At gene . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| The overexpression of either eEF1A or eEF1B alpha , the catalytic subunit of the guanine nucleotide exchange factor , in Saccharomyces cerevisiae results in effects on cell growth . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| Several of these proteins are subject to phosphorylation in mammalian cells , including the factors eEF1A and eEF1B that are involved in recruitment of amino acyl tRNAs to the ribosome . eEF 2 , which mediates ribosomal translocation , is also phosphorylated and this inhibits its activity . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| We found that the vast majority of translation initiation factors ( eIF 2 , eIF2B , eIF 3 , eIF4A1 , eIF 5 and eIF5B ) , all three elongation factors ( eEF1A , eEF1B and eEF 2 ) and the termination factor eRF 1 are strictly excluded from nuclei . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| The multi subunit eukaryotic translation elongation factor 1 ( eEF 1 ) consists of two functionally distinct parts : G protein eEF1A and guanine nucleotide exchange factor eEF1B . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| Elongation factor 1 ( eEF 1 ) , which is responsible for aminoacyl tRNA transfer on the ribosome , is comprised of two entities : a G protein named eEF1A and a nucleotide exchange factor , eEF1B . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| To sustain efficient translation , eukaryotic elongation factor B alpha ( eEF1B alpha ) functions as the guanine nucleotide exchange factor for eEF1A . ^^^ Stopped flow kinetics using 2 ' ( or 3 ' ) O N methylanthraniloyl ( mant ) GDP showed spontaneous release of nucleotide from eEF1A is extremely slow and accelerated 700 fold by eEF1B alpha . ^^^ Co crystal structures of the lethal K205A mutant in the catalytic C terminus of eEF1B alpha with eEF1A and eEF1A . ^^^ |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P24534 and P68104 |
Pubmed |
SVM Score :0.0 |
| NA |
|