| Interacting proteins: P12931 and P63244 |
Pubmed |
SVM Score :0.0 |
| Furthermore , immunoblotting confirmed hypothesized changes in other proteins in the proposed network : Increases in c src , receptor for activated C kinase 1 , calreticulin , and caspase 3 and decreases in steroid receptor co activator 1 , Grp78 / BiP , and annexin A 4 . ^^^ |
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| Interacting proteins: P12931 and P63244 |
Pubmed |
SVM Score :0.0 |
| RACK 1 , a receptor for activated C kinase and a homolog of the beta subunit of G proteins , inhibits activity of src tyrosine kinases and growth of NIH 3T3 cells . ^^^ We identified RACK 1 , a receptor for activated C kinase and a homolog of the beta subunit of G proteins , as a Src binding protein . ^^^ Using GST Src fusion proteins , we determined that RACK 1 binds to the SH 2 domain of Src . ^^^ Coimmunoprecipitation of Src and RACK 1 was demonstrated with NIH 3T3 cells . ^^^ Purified GST RACK 1 inhibited the in vitro kinase activity of Src in a concentration dependent manner . ^^^ |
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| Interacting proteins: P12931 and P63244 |
Pubmed |
SVM Score :0.0 |
| We found that RACK 1 , a molecule associating with activated PKC , PLCgamma and Src kinases , associated with the membrane proximal region of betac in both yeast two hybrid , immunoprecipitation and GST pull down assays . ^^^ |
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| Interacting proteins: P12931 and P63244 |
Pubmed |
SVM Score :0.0 |
| Since RACK 1 modulates signaling from protein kinase C and Src tyrosine kinases , the results suggest a possible role for CST products in growth control , perhaps consistent with the abundant transcription of CST RNAs in cancers such as nasopharyngeal carcinoma . . ^^^ |
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| Interacting proteins: P12931 and P63244 |
Pubmed |
SVM Score :0.0 |
| In mammalian cells , RACK 1 physically associates with some signal transduction proteins , including Src and protein kinase C . ^^^ |
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| Interacting proteins: P12931 and P63244 |
Pubmed |
SVM Score :0.0 |
| RACK 1 is known to bind to the src protein tyrosine kinase . ^^^ This study demonstrates that PTPmu association with RACK 1 is disrupted by the presence of constituitively active src . ^^^ |
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| Interacting proteins: P12931 and P63244 |
Pubmed |
SVM Score :0.0 |
| The interaction of Src and RACK 1 is enhanced by activation of protein kinase C and tyrosine phosphorylation of RACK 1 . ^^^ Previously , we demonstrated that RACK 1 also interacts with the Src protein tyrosine kinase . ^^^ To further characterize the Src RACK 1 interaction and to analyze mechanisms by which cross talk occurs between the two RACK 1 linked signaling kinases , we identified sites on Src and RACK 1 that mediate their binding , and factors that regulate their interaction . ^^^ We found that the interaction of Src and RACK 1 is mediated , in part , by the SH 2 domain of Src and by phosphotyrosines in the sixth WD repeat of RACK 1 , and is enhanced by serum or platelet derived growth factor stimulation , protein kinase C activation , and tyrosine phosphorylation of RACK 1 . ^^^ |
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| Interacting proteins: P12931 and P63244 |
Pubmed |
SVM Score :0.0 |
| The scaffolding protein , Rack 1 , is a seven WD domain containing protein that has been implicated in binding to integrin beta subunit cytoplasmic domains and to members of two kinase families ( src and protein kinase C , PKC ) that mediate integrin bidirectional signaling . ^^^ These effects on motility and cytoskeletal organization did not appear to result from Rack 1 inhibition of src function as downstream substrates of this kinase were phosphorylated normally . ^^^ In addition , expression of an active src construct did not reverse the migratory deficit induced by Rack 1 overexpression . ^^^ |
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| Interacting proteins: P12931 and P63244 |
Pubmed |
SVM Score :0.0 |
| RACK 1 , the anchoring protein for activated betaIIPKC , binds for example , Src tyrosine kinase , integrin , and phosphodiesterase . ^^^ |
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| Interacting proteins: P12931 and P63244 |
Pubmed |
SVM Score :0.0 |
| Src , the p 85 subunit of phosphatidylinositol 3 kinase , and SHP 2 were all associated with RACK 1 in these cells . ^^^ |
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| Interacting proteins: P12931 and P63244 |
Pubmed |
SVM Score :0.0 |
| RACK 1 : a novel substrate for the Src protein tyrosine kinase . ^^^ Previously , we showed that RACK 1 also interacts with the Src tyrosine kinase , and is an inhibitor of Src activity and cell growth . ^^^ PKC activation induces the intracellular movement and co localization of RACK 1 and Src , and the tyrosine phosphorylation of RACK 1 . ^^^ To determine whether RACK 1 is a Src substrate , we assessed phosphorylation of RACK 1 by various tyrosine kinases in vitro , and by kinase active and inactive mutants of Src in vivo . ^^^ We found that RACK 1 is a Src substrate . ^^^ |
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| Interacting proteins: P12931 and P63244 |
Pubmed |
SVM Score :0.0 |
| Targeting occurs by association with proteins , such as arrestins , SRC family tyrosyl kinases , A kinase anchoring proteins ( ' AKAPs ' ) and receptor for activated C kinase 1 ( ' RACK1 ' ) , and , in the case of isoform PDE4A1 , by a specific interaction ( TAPAS 1 ) with phosphatidic acid . ^^^ |
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| Interacting proteins: P12931 and P63244 |
Pubmed |
SVM Score :0.0 |
| RACK 1 regulates integrin mediated adhesion , protrusion , and chemotactic cell migration via its Src binding site . ^^^ The identified cDNA was a C terminal truncated form of RACK 1 that had one of the putative protein kinase C binding sites but lacked the region proposed to bind the beta integrin cytoplasmic domain and the tyrosine kinase Src . ^^^ To investigate the role of RACK 1 during cell spreading and migration , we tagged RACK 1 , a C terminal truncated RACK 1 and a point mutant that does not bind Src ( RACK Y246F ) with green fluorescent protein and expressed them in CHO K 1 cells . ^^^ We also found that RACK 1 regulates cell protrusion and chemotactic migration through its Src binding site . ^^^ Together , these findings suggest that RACK 1 regulates adhesion , protrusion , and chemotactic migration through its interaction with Src . . ^^^ |
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| Interacting proteins: P12931 and P63244 |
Pubmed |
SVM Score :0.0 |
| Furthermore , we provide evidence that E1A is able to antagonize the inhibitory effects of RACK 1 on Src activity . ^^^ |
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| Interacting proteins: P12931 and P63244 |
Pubmed |
SVM Score :0.0 |
| RACK 1 inhibits the serum and anchorage independent growth of 5 Src transformed cells . ^^^ Previously , we showed that RACK 1 inhibits c Src kinase activity and NIH3T3 cell growth . ^^^ Here , we show that RACK 1 partially inhibits 5 Src kinase activity , and the serum and anchorage independent growth of 5 Src transformed cells , but has no effect on focus formation . ^^^ RACK 1 overexpressing 5 Src cells show disassembly of podosomes , which are actin rich structures that are distinctive to fully transformed cells . ^^^ Together , our results demonstrate that RACK 1 overexpression in 5 Src cells partially reverses the transformed phenotype of the cells . ^^^ |
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| Interacting proteins: P12931 and P63244 |
Pubmed |
SVM Score :0.0 |
| In R cells transiently expressing the Y1250F / Y1251F mutant RACK 1 became constitutively associated with beta 1 integrin and did not associate with Shc , Src , or Shp 2 . ^^^ This was accompanied by the loss of formation of a complex containing the IGF IR , RACK 1 , and beta 1 integrin ; loss of migratory capacity ; enhanced Src and FAK activity ; enhanced Akt phosphorylation ; and decreased p 38 mitogen activated protein kinase activity . ^^^ |
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| Interacting proteins: P12931 and P63244 |
Pubmed |
SVM Score :0.0 |
| And , RACK 1 protein was reactive with syndecan 2 to keep Src inactivated , but not when Ras was overexpressed . ^^^ We found that RACK 1 was dimerized upon reaction with GTP K ( B ) Ras ( Q61K ) , as well as with 14 3 3beta and geranylgeranyl pyrophosphate , as revealed by phosphorylation with Src tyrosine kinase . ^^^ |
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| Interacting proteins: P12931 and P63244 |
Pubmed |
SVM Score :0.0 |
| Trap RACK 1 with Ras to mobilize Src signaling at syndecan 2 / p120 GAP upon transformation with oncogenic ras . ^^^ The selective molecular affinity for Src was found to be stronger with HiTrap syndecan 2 / RACK1 , as revealed with competitive RACK 1 to dislodge Src from HiTrap syndecan 2 / p120 GAP . ^^^ The reaction between GTP K ( B ) Ras ( Q ( 61 ) K ) and RACK 1 could provide a mechanism to deprive RACK 1 for the organization of syndecan 2 / RACK1 complex and to facilitate the formation of syndecan 2 / p120 GAP complex , as well as to provide docking sites for Src signaling upon transformation with oncogenic ras . . ^^^ |
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| Interacting proteins: P12931 and P63244 |
Pubmed |
SVM Score :0.0 |
| Peptides that mimic the binding sites for either Fyn or Src on receptor for activated C kinase 1 ( RACK 1 ) also enhanced NMDAR in CA 1 neurons , but their effects were blocked by Src ( 40 58 ) , implying that Src is the ultimate regulator of NMDARs . ^^^ RACK 1 serves as a hub for PKC , Fyn , and Src and facilitates the regulation of basal NMDAR activity in CA 1 hippocampal neurons . . ^^^ |
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| Interacting proteins: P12931 and P63244 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P63244 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P63244 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P63244 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P12931 and P63244 |
Pubmed |
SVM Score :0.0 |
| NA |
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