| Interacting proteins: Q13309 and P63208 |
Pubmed |
SVM Score :0.63435119 |
| RESULTS : We identified specific amino acid residues ( I 143 and L 152 ) within the F box of HOS that play a critical role in maintaining the hydrophobic interface of HOS Skp 1 interaction and found substantial similarity between interaction of Skp 1 with HOS and with another F box protein Skp 2 . 0.63435119^^^ |
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| Interacting proteins: Q13309 and P63208 |
Pubmed |
SVM Score :0.75868766 |
| We show that Skp 1 can bind to Skp 2 in vitro using recombinant proteins , and in vivo using the yeast two hybrid system . 0.75868766^^^ Here we characterized the interaction between Skp 1 and the F box protein Skp 2 . 0.5870862^^^ |
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| Interacting proteins: Q13309 and P63208 |
Pubmed |
SVM Score :0.0 |
| Regulation of cyclin A Cdk 2 by SCF component Skp 1 and F box protein Skp 2 . ^^^ |
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| Interacting proteins: Q13309 and P63208 |
Pubmed |
SVM Score :0.0 |
| The F box protein Skp 2 is important for S phase entry and binds to Skp 1 and the cyclin A Cdk 2 complex . ^^^ |
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| Interacting proteins: Q13309 and P63208 |
Pubmed |
SVM Score :0.0 |
| The ubiquitin protein ligase SCF ( Skp 2 ) is composed of Skp 1 , Cul 1 , Roc1 / Rbx1 and the F box protein Skp 2 , the substrate recognition subunit . ^^^ |
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| Interacting proteins: Q13309 and P63208 |
Pubmed |
SVM Score :0.0 |
| Here we describe the crystal structure of the human F box protein Skp 2 bound to Skp 1 . ^^^ |
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| Interacting proteins: Q13309 and P63208 |
Pubmed |
SVM Score :0.0 |
| In contrast to substrates of the SKP 1 Cullin 1 F box ( SCF ) complexes , in vitro ubiquitination of E2F1 by CUL 1 ROC1 ligase does not require E2F1 phosphorylation , is not stimulated by overexpression of F box protein SKP 2 , and is not affected by immunodepletion of SKP 1 or mutations in CUL 1 disrupting SKPI binding . ^^^ |
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| Interacting proteins: Q13309 and P63208 |
Pubmed |
SVM Score :0.0 |
| Whereas , the Skp 1 component of the SCF complex is overexpressed in senescent fibroblasts , the abundance of the F box protein Skp 2 is strongly reduced . ^^^ |
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| Interacting proteins: Q13309 and P63208 |
Pubmed |
SVM Score :0.0 |
| We found the majority of CUL 1 is in a complex with CAND 1 and ROC 1 independent of SKP 1 and F box protein SKP 2 . ^^^ |
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| Interacting proteins: Q13309 and P63208 |
Pubmed |
SVM Score :0.0 |
| The F box protein SKP 2 promotes the G 1 S transition by targeting key regulators for proteasomal degradation via its capacity to function as the specificity factor for the SKP 1 Cullin F box SCF ( SKP 2 ) ubiquitin ligase . ^^^ |
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| Interacting proteins: Q13309 and P63208 |
Pubmed |
SVM Score :0.0 |
| According to a current model , the ubiquitination of p 27 during S phase progression is mediated by SCF ( Skp 2 ) E 3 ligase that captures Thr 187 phosphorylated p 27 by means of the F box protein Skp 2 , which in turn couples the bound substrate via Skp 1 to a catalytic core complex composed of Cul 1 and the Rbx / Roc RING finger protein . ^^^ |
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| Interacting proteins: Q13309 and P63208 |
Pubmed |
SVM Score :0.0 |
| Beach , p19Skp1 and p45Skp2 are essential elements of the cyclin A CDK 2 S phase kinase , Cell , 82 ( 1995 ) 915 925 . ] . ^^^ |
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| Interacting proteins: Q13309 and P63208 |
Pubmed |
SVM Score :0.0 |
| We describe here the mapping by fluorescence in situ hybridization of the gene for the CDK2 / cyclin A associated protein p 45 ( SKP 2 ) to 5p13 and the p 19 related genes p19A ( SKP1A ) and p19B ( SKP1B ) to 7q11 . 2 and 12p12 , respectively . ^^^ Chromosomal mapping of the genes for the human CDK2 / cyclin A associated proteins p 19 ( SKP1A and SKP1B ) and p 45 ( SKP 2 ) . ^^^ |
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| Interacting proteins: Q13309 and P63208 |
Pubmed |
SVM Score :0.0 |
| In normal and transformed cells , the F box protein p 45 ( SKP 2 ) is required for S phase and forms stable complexes with p 19 ( SKP 1 ) and cyclin A cyclin dependent kinase ( CDK ) 2 . ^^^ CUL 1 also associates with cyclin A and p 19 ( SKP 1 ) in vivo and , with p 45 ( SKP 2 ) , they assemble into a large multiprotein complex . ^^^ The data presented here imply that the p 45 ( SKP 2 ) CUL 1 p 19 ( SKP 1 ) complex may be a human representative of an SCF type E 3 ubiquitin protein ligase . ^^^ Finally , we show that multiprotein complex formation involving p 45 ( SKP 2 ) CUL 1 and p 19 ( SKP 1 ) is governed , in part , by periodic , S phase specific accumulation of the p 45 ( SKP 2 ) subunit and by the p 45 ( SKP 2 ) bound cyclin A CDK 2 . ^^^ The dependency of p 45 ( SKP 2 ) p 19 ( SKP 1 ) complex formation on cyclin A CDK 2 may ensure tight coordination of the activities of the cell cycle clock with those of a potential ubiquitin conjugation pathway . . ^^^ |
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| Interacting proteins: Q13309 and P63208 |
Pubmed |
SVM Score :0.0 |
| Human CUL 1 , but not other cullin family members , selectively interacts with SKP 1 to form a complex with SKP 2 and cyclin A . ^^^ |
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| Interacting proteins: Q13309 and P63208 |
Pubmed |
SVM Score :0.0 |
| In many transformed cells , the cyclin A / CDK2 complex that contains S phase kinase associated proteins 1 and 2 ( SKP 1 and SKP 2 ) is highly induced . ^^^ The identification of CUL 1 as a member of the complex raises the possibility that the p 19 ( SKP 1 ) / p45 ( SKP 2 ) / CUL 1 complex may function as the yeast SKP 1 CDC53 F box ( SCF ) protein complex that acts as a ubiquitin E 3 ligase to regulate the G1 / S transition . ^^^ To determine the potential in vivo targets of the p 19 ( SKP 1 ) / p45 ( SKP 2 ) / CUL 1 complex , we have used the specific antisense oligodeoxynucleotides against either SKP 1 , SKP 2 , or CUL 1 RNA to inhibit their expression . ^^^ These data suggest that the human p 19 ( SKP 1 ) / p45 ( SKP 2 ) / CUL 1 complex is likely to function as an E 3 ligase to selectively target cyclin D and p 21 for the ubiquitin dependent protein degradation . ^^^ Aberrant expression of human p 19 ( SKP 1 ) / p45 ( SKP 2 ) / CUL 1 complex thus may contribute to tumorigenesis by regulating the protein levels of G 1 cell cycle regulators . . ^^^ |
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| Interacting proteins: Q13309 and P63208 |
Pubmed |
SVM Score :0.0 |
| Association of human SCF ( SKP 2 ) subunit p 19 ( SKP 1 ) with interphase centrosomes and mitotic spindle poles . ^^^ |
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| Interacting proteins: Q13309 and P63208 |
Pubmed |
SVM Score :0.0 |
| Immunodepletion of components of the complex Cul 1 , Skp 1 , or Skp 2 from the extract abolished p 27 degradation , while addition of purified SCF ( Skp 2 ) to Skp 2 depleted extract restored the capacity to degrade p 27 . ^^^ Skp 2 dependent associations between Skp 1 or Cul 1 and the p 27 phosphopeptide were also detected . ^^^ |
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| Interacting proteins: Q13309 and P63208 |
Pubmed |
SVM Score :0.0 |
| Ubiquitinylation of p 27 requires its phosphorylation at Thr 187 ( refs 3 , 4 ) and subsequent recognition by S phase kinase associated protein 2 ( Skp 2 ; refs 5 8 ) , a member of the F box family of proteins that associates with Skp 1 , Cul 1 and ROC1 / Rbx1 to form an SCF ubiquitin ligase complex . ^^^ |
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| Interacting proteins: Q13309 and P63208 |
Pubmed |
SVM Score :0.0 |
| We have reported previously that the degradation of p 27 requires its phosphorylation on Thr 187 and is mediated by Skp 2 , an F box protein that associates with Skp 1 , Cul 1 , and Roc1 / Rbx1 to form the SCF ( Skp 2 ) ubiquitin ligase complex . ^^^ |
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| Interacting proteins: Q13309 and P63208 |
Pubmed |
SVM Score :0.0 |
| We found that p 130 interacts with SKP 1 , Cul 1 and SKP 2 in human 293 cells . ^^^ |
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| Interacting proteins: Q13309 and P63208 |
Pubmed |
SVM Score :0.0 |
| The Skp 2 oncoprotein belongs to the family of F box proteins that function as substrate recognition factors for SCF ( Skp 1 , cullin , F box protein ) E 3 ubiquitin ligase complexes . ^^^ |
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| Interacting proteins: Q13309 and P63208 |
Pubmed |
SVM Score :0.0 |
| The SCF ( Skp 2 ) complex ( consisting of Rbx 1 , Cul 1 , Skp 1 , and Skp 2 ) is one of the E 3 ubiquitin ligases involved in ubiquitination of p 21 ( Cip1 / WAF1 ) . ^^^ |
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| Interacting proteins: Q13309 and P63208 |
Pubmed |
SVM Score :0.0 |
| Ubiquitination of p 27 ( kip 1 ) is performed by the SCF ( skp 2 ) ubiquitin ligase comprised of the core components Roc 1 , Cul 1 and Skp 1 and the substrate recognition components Skp 2 and Cks 1 . ^^^ Here we show that in primary human T lymphocytes , the SCF ( skp 2 ) core components Roc 1 , Cul 1 and Skp 1 are constitutively expressed , and their levels remain unchanged upon TCR / CD3 plus CD 28 costimulation . ^^^ |
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| Interacting proteins: Q13309 and P63208 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13309 and P63208 |
Pubmed |
SVM Score :0.0 |
| NA |
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