| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :1.2476728 |
| When stably expressed in K 562 cells , SHIP was found to be constitutively tyrosine phosphorylated and associated with endogenous Shc and Grb 2 . 1.2476728^^^ |
|
| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.66536826 |
| In summary , 1 ) SHIP WT and SHIPDeltaIP expression inhibit insulin and PDGF stimulated Ras , MAPK kinase , and MAPK activities ; 2 ) SHIP associates with tyrosine phosphorylated Shc , and the proline rich sequences in SHIP associate with Grb 2 and titrate out SOS to form Shc * Grb2 * SHIP complexes ; and 3 ) dissociation of SOS from the Shc * Grb2 complex inhibits Ras GTP loading , leading to decreased signaling through the MAPK pathway . . 0.66536826^^^ |
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| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| By using an independent approach , expression cloning , we found that the Grb 2 SH3 domains bind specifically to SIP 110 , a 110 kDa splice variant of SIP 145 and SIP 130 , which lacks the SH 2 domain . ^^^ |
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| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| SHIP is constitutively tyrosine phosphorylated and associated with Shc and Grb 2 . ^^^ |
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| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| The insulin regulated polyphosphoinositide 5 ' phosphatase was not immunoreactive with antibody raised against the recently cloned SHIP 5 ' phosphatase reported to associate with Shc and Grb 2 in B lymphocytes . ^^^ |
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| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| Signaling factors that associate with activated erythropoietin ( Epo ) receptor complexes include phospholipase C gamma , phosphatidylinositol 3 kinase , SHIP , Shc , Grb 2 , Cbl , Crk l , HCP , Syp , and STAT 5 . ^^^ |
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| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| The 100 kDa protein did not appear to bind directly to Fms , Ship , Cbl , Shc , or Grb 2 , although all of these proteins were coimmunoprecipitated with p 85 after M CSF stimulation . ^^^ |
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| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| We previously observed that this stimulation condition is associated with a reduction in the interaction of Grb 2 with phosphorylated Shc , an enhanced interaction of Shc with SHIP , and a block in the Ras signaling pathway . ^^^ We proposed that the SH 2 domain of SHIP competes with Grb 2 in binding to phospho Shc , resulting in a block in Ras signaling . ^^^ These findings are consistent with our earlier model that SHIP and Grb 2 compete for binding to phospho Shc and support the notion that , in addition to the hydrolysis of inositol phosphates and phospholipids , SHIP contributes to anti proliferative biochemistry by blocking protein protein interactions . . ^^^ |
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| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| Negative signaling through SHIP appears to inhibit the Ras pathway through SH 2 domain competition with Grb 2 and Shc and may involve consumption of intracellular lipid mediators that act as allosteric enzyme activators or that promote entry of extracellular Ca2+ . . ^^^ |
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| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| After macrophage colony stimulating factor ( M CSF ) stimulation of FDC P 1 ( Fms ) myeloid cells , both 145 and 135 kD SHIP forms were tyrosine phosphorylated and could be coimmunoprecipitated with antibodies to Shc and Grb 2 . ^^^ |
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| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| Flt 3 signaling involves tyrosyl phosphorylation of SHP 2 and SHIP and their association with Grb 2 and Shc in Baf3 / Flt3 cells . ^^^ These results demonstrate that SHP 2 and SHIP are important components in the human Flt 3 signaling pathway and suggest that SHP 2 and SHIP , by forming complexes with adapter proteins Grb 2 and Shc , may modulate MAP kinase activation , which may be necessary for the mitogenic signaling of Flt3 . . ^^^ |
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| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| Furthermore , in contrast to SHIP 1 , SHIP 2 did not bind to either the N terminal or C terminal SH 3 domains of GRB 2 . ^^^ |
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| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| The src homology domain 2 containing inositol phosphatase SHIP forms a ternary complex with Shc and Grb 2 in antigen receptor stimulated B lymphocytes . ^^^ Here , we report that cross linking of the BCR induces association between Grb 2 and SHIP as well as association between Shc and SHIP . ^^^ We made use of a Grb 2 deficient B cell line to demonstrate both in vitro and in vivo that Grb 2 expression is required for the efficient association between Shc and SHIP . ^^^ The results indicate that SHIP , Shc , and Grb 2 form a ternary complex in stimulated B cells , with Grb 2 stabilizing the interaction between Shc and SHIP . ^^^ The interactions between Shc , Grb 2 , and SHIP are therefore analogous to the interactions between Shc , Grb 2 , and SOS . ^^^ |
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| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| Although overexpression of SHIP 2 did not affect insulin induced tyrosine phosphorylation of the insulin receptor beta subunit and Shc , subsequent association of Shc with Grb 2 was inhibited , possibly by competition between the SH 2 domains of SHIP 2 and Grb 2 for the Shc phosphotyrosine . ^^^ Grb 2 complex and of the downstream products of PI 3 kinase provides possible mechanisms of SHIP 2 action in insulin signaling . . ^^^ |
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| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| Phosphorylated Tyr 317 , and to a lesser extent , Tyr 239 , bound the Grb 2 adaptor and SHIP . ^^^ Furthermore , a pTyr 317 Shc phosphopeptide selectively recognized Grb 2 , Sos 1 , SHIP , and the p 85 subunit of phosphatidylinositol 3 ' kinase from mast cells , as characterized by mass spectrometry . ^^^ |
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| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| In this report , we identify this 100 kDa protein as the recently cloned scaffolding protein Gab 2 , and we demonstrate that Gab 2 associates with several molecules involved in M CSF signaling , including Grb 2 , SHP 2 , the p 85 subunit of phosphatidylinositol 3 ' kinase , SHIP , and SHC . ^^^ |
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| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| In embryonic stem cells , s SHIP partners with the adapter protein Grb 2 without tyrosine phosphorylation and is present constitutively at the cell membrane . ^^^ |
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| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| In these cells , PI3K was constitutively associated with the molecular adapters Grb 2 and Gab 1 , and with the phosphatases SHP 2 and SHIP . ^^^ |
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| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| In contrast , SHIP phosphorylation was not inhibited by a dominant negative mutant of Grb 2 . ^^^ |
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| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| Although phosphorylation of Gab 2 was similar in WT and mutant cell lines , phosphorylation of Shc on Tyr ( 313 ) in response to IGF 1 was decreased in cells expressing the mutant receptor , as was recruitment of Grb 2 and Ship to Shc . ^^^ |
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| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| We therefore hypothesized a competition model in which the SH 2 domain of SHIP competes with that of Grb 2 for binding to phospho Shc to inhibit the Ras pathway . ^^^ Second , under conditions of negative signaling leading to SHIP Shc interaction in wild type B cells , we observed a profound reduction in the activation induced association of Grb 2 to Sos . ^^^ Third , we demonstrated that phospho Shc can not concomitantly bind Grb 2 and SHIP , indicating that the two proteins competed for the same phospho tyrosine residue on Shc . ^^^ |
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| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| Our results provide the first evidence that FcalphaR mediated signals modulate a multimolecular adaptor protein complex containing Grb 2 , Shc , SHIP , CrkL , Cbl , and SLP 76 . ^^^ These data indicate that the stimulation of FcalphaR results in the modulation of adaptor complexes containing tyrosine phosphorylated Cbl , Shc , SHIP , Grb 2 , and Crkl . ^^^ |
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| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| Insulin stimulation of Shc association with Grb 2 , which is important for p21ras MAP kinase activation , was decreased by overexpression of WT and 2F SHIP . ^^^ Importantly , insulin induced Shc 10 Grb 2 association was not detectably reduced in deltaSH 2 SHIP cells . ^^^ In accordance with the extent of Shc association with Grb 2 , insulin induced MAP kinase activation was relatively decreased in both WT SHIP and 2F SHIP cells , but not in deltaSH 2 SHIP cells . ^^^ |
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| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| CD 22 forms a quaternary complex with SHIP , Grb 2 , and Shc . ^^^ |
|
| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| Co aggregation of FcgammaRII with FcepsilonRI on human mast cells inhibits antigen induced secretion and involves SHIP Grb 2 Dok complexes . ^^^ Co aggregating FcepsilonRI and FcgammaRII with GE 2 1 ) significantly inhibited IgE mediated histamine release , cytokine production , and Ca ( 2+ ) mobilization , 2 ) reduced the antigen induced morphological changes associated with mast cell degranulation , 3 ) reduced the tyrosine phosphorylation of several cellular substrates , and 4 ) increased the tyrosine phosphorylation of the adapter protein downstream of kinase 1 ( p 62 ( dok ) ; Dok ) , growth factor receptor bound protein 2 ( Grb 2 ) , and SH 2 domain containing inositol 5 phosphatase ( SHIP ) . ^^^ Surprisingly , in non stimulated cells , there were complexes of phosphorylated SHIP Grb 2 Dok that were lost upon IgE receptor activation but retained under conditions of Fcepsilon Fcgamma co aggregation . ^^^ Our results demonstrate how human FcepsilonRI mediated responses can be inhibited by co aggregation with FcgammaRIIB and implicate Dok , SHIP , and Grb 2 as key intermediates in regulating antigen induced mediator release . . ^^^ |
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| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| Both Grb 2 and Grap are required for an optimal coprecipitation of SHIP with FcgammaRIIB , but one adapter is sufficient for the phosphatase to coprecipitate in a detectable manner with the receptors . ^^^ |
|
| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| EPO activates the tyrosine phosphorylation of Ship 1 , resulting in the interdependent recruitment of Shc and Grb 2 . ^^^ In addition , we have shown that EPO stimulates the formation of a ternary complex consisting of Ship 1 , Shc , and Grb 2 . ^^^ |
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| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| TEL JAK 2 expression in Ba / F3 cells results in constitutive association and tyrosine phosphorylation of Shc and Ship 1 and , consequently , recruitment of Grb 2 to TEL JAK 2 . ^^^ |
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| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| To conduce these activities , the cytoplasmic C terminal region of this receptor acts as a docking site for multiple protein substrates , including Grb 2 , Gab 1 , STAT 3 , Shc , SHIP 1 and Src . ^^^ |
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| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92835 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
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