| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.68674713 |
| We propose that Pyk 2 acts with Src to link Gi and Gq coupled receptors with Grb 2 and Sos to activate the MAP kinase signalling pathway in PC 12 cells . . 0.68674713^^^ |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| ANG 2 ( 100 nM ) promoted the formation of a complex between PYK 2 and the ERK1 / 2 regulators Shc and Grb 2 . ^^^ |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| In apoptotic cells , the expressions of the PYK 2 , p p 38 , PI3K and Grb 2 adhesion proteins were increased . ^^^ |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| Mutation of Tyr 881 , a putative Src kinase phosphorylation site predicted to bind Grb 2 , had little effect on CADTK regulation . ^^^ |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| Involvement of proline rich tyrosine kinase 2 in platelet activation : tyrosine phosphorylation mostly dependent on alphaIIbbeta 3 integrin and protein kinase C , translocation to the cytoskeleton and association with Shc through Grb 2 . ^^^ |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| Presently , we found that glucose and certain sugars rapidly activated extracellular signal regulated kinase ( ERK ) by a mechanism that was : ( a ) independent of glucose uptake / metabolism and protein kinase C but nevertheless cytochalasin B inhibitable ; ( b ) dependent upon proline rich tyrosine kinase 2 ( PYK 2 ) , GRB 2 , SOS , RAS , RAF , and MEK 1 ; and ( c ) amplified by overexpression of the Glut 1 , but not Glut 2 , Glut 3 , or Glut 4 , glucose transporter . ^^^ |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| Presently , we found that glucose acutely activated PKC zeta / lambda in rat adipocytes and rat skeletal muscle preparations by a mechanism that was independent of phosphatidylinositol 3 kinase but , interestingly , dependent on the apparently sequential activation of the dantrolene sensitive , nonreceptor proline rich tyrosine kinase 2 ; components of the extracellular signal regulated kinase ( ERK ) pathway , including , GRB 2 , SOS , RAS , RAF , MEK 1 and ERK1 / 2 ; and , most interestingly , phospholipase D , thus yielding increases in phosphatidic acid , a known activator of PKC zeta / lambda . ^^^ |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| In CTL , phosphorylation of proline rich tyrosine kinase 2 downstream of integrin stimulation did not result in recruitment of the adaptor protein Grb 2 . ^^^ |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| Analysis of tyrosine phosphorylated RAFTK from adherent transfected COS cells showed that the Src homology 2 ( SH 2 ) domains of the Src and Fyn protein kinases as well as the Grb 2 adaptor protein were able to specifically associate with RAFTK . ^^^ These data suggest that RAFTK is a novel member of the FAK family , that it localizes to focal adhesion like structures in CMK megakaryocytic cells , that it participates in integrinmediated signaling pathways in megakaryocytes , and that it is able to associate with the tyrosine kinases Src and Fyn as well as the adaptor protein Grb 2 via SH 2 phosphotyrosine interactions . . ^^^ |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| RAFTK was phosphorylated and activated upon VRP treatment , and there was an enhanced association of this kinase with the adaptor protein Grb 2 . ^^^ |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| These results suggest that the P2Y2 receptor initiated activation of MAP kinase was dependent on the elevation of [ Ca2+ ] 1 , involved the recruitment of Shc and Grb 2 , and was mediated by RAFTK and PKC . . ^^^ |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| AG 1478 , a tyrphostin selective for the EGF receptor , reduced the activation of MAP kinase , the tyrosine phosphorylation of SHC , the association of Grb 2 with SHC , and the tyrosine phosphorylation of the EGF receptor and p 120 ( cbl ) but did not block the tyrosine phosphorylation of RAFTK . ^^^ |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| In contrast , grb 2 heterozygosity did not alter tumorigenesis in either pten+ / or T cell specific pten / mice . grb 2 / hypomorph murine embryonic fibroblasts ( MEFs ) show decreased activation of both PKB and Erk upon stimulation with epidermal growth factor , whereas grb 2 / hypomorph ; pten+ / MEFs activate PKB but not Erk normally . ^^^ Activation of both PKB and Erk as well as survival in low serum containing media are all rescued by reexpression of Grb 2 containing mutations within the N terminal Src homology 3 ( SH 3 ) domain , but not by C terminal SH 3 domain mutants . ^^^ In fibroblasts , survival signals emanating from the epidermal growth factor receptor appear to be PKB dependent , and this activation depends on the C terminal SH 3 domain of Grb 2 , likely through the interaction of Grb 2 with Gab1 . . ^^^ |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| Previous studies have demonstrated that GRB 2 directly interacts with Shc , a proto oncogene product that is tyrosine phosphorylated upon receptor and nonreceptor PTK activation . ^^^ |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| The object of the present study was to explore the regulation of the SH 2 and SH 3 domain containing adapter molecule Grb 2 by Fc epsilon R 1 stimulated PTK signal transduction pathways . ^^^ |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| Here we show that adhesion of murine NIH3T3 fibroblasts to fibronectin promotes SH 2 domain mediated association of the GRB 2 adaptor protein and the c Src protein tyrosine kinase ( PTK ) with FAK in vivo , and also results in activation of mitogen activated protein kinase ( MAPK ) . ^^^ |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| Ligation of the T cell antigen receptor results in the activation of both a PTK , and Ras ; therefore , we investigated whether Grb 2 may serve a similar function in T cells . ^^^ |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| The data show that the doubly phosphorylated ITAM all bind the PTK ZAP 70 , but the ITAM also variably bind the PTK p59fyn and the adapters Shc , Grb 2 and the p 85 regulatory subunit of phosphoinositol 3 ' kinase . ^^^ |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| We showed previously that the c Src nonreceptor PTK and the Grb 2 SH2 / SH3 adaptor protein bound directly to FAK after fibronectin stimulation ( D . ^^^ |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| In contrast , Shc , phosphatidylinositol 3 kinase ( Pl3K ) , Grb 2 , and Ras GTPase activating protein ( GAP ) bound with different affinities to the bis or monophosphorylated peptides , while the Src family protein tyrosine kinase ( PTK ) Fyn did not bind specifically to any of the tested peptides . ^^^ |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| Treatment of fibroblasts with protein kinase C inhibitors or with the PTK inhibitor herbimycin A or PP 1 resulted in reduced Src PTK activity , no Grb 2 binding to FAK , and lowered levels of ERK 2 activation . ^^^ |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| Significantly , repression of endogenous Src family PTK activity by p 50 ( csk ) overexpression inhibited FN stimulated cell spreading , Pyk 2 tyrosine phosphorylation , Grb 2 binding to Shc , and ERK 2 activation in the FAK but not in FAK+ cells . ^^^ |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| Upon T cell antigen receptor ( TCR / CD3 ) engagement , LAT is phosphorylated by protein tyrosine kinases ( PTK ) and binds to the adaptors Gads and Grb 2 , as well as to phospholipase Cgamma 1 ( PLCgamma 1 ) , thereby facilitating the recruitment of key signal transduction components to drive T cell activation . ^^^ |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| These tribrid systems function by expression of the PTK , a B 42 activation domain fused to the phosphotyrosine binding Grb 2 SH2 domain , a DNA bound LexA GFP ( AAYANAA ) ( 4 ) universal PTK substrate , and a lacZ reporter gene . ^^^ |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| Furthermore , we identify two tyrosine residues that are subject to phosphorylation in response to muscarinic signaling and show that this phosphorylation induces two cytosolic proteins , c Src and Grb 2 , to bind to PYK 2 . ^^^ |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| Although a selective EGFR kinase inhibitor completely abolished Ang 2 induced recruitment of Grb 2 to EGFR and markedly attenuated Ang 2 induced ERK activation , it had no effect on Ang 2 induced PYK 2 tyrosine phosphorylation or its association with c Src and Grb 2 . ^^^ These data suggest that the AT 1 receptor uses Ca2+ dependent PYK 2 to activate c Src , thereby leading to EGFR transactivation , which preponderantly recruits Grb 2 in rat VSMC . . ^^^ |
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| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| Adaptor proteins Grb 2 and Crk couple Pyk 2 with activation of specific mitogen activated protein kinase cascades . ^^^ In this report , we provide evidence that the protein tyrosine kinase Src and adaptor proteins Grb 2 , Crk , and p130Cas act as downstream mediators of Pyk 2 leading to the activation of extracellular signal regulated kinase ( ERK ) and c Jun amino terminal kinase ( JNK ) . ^^^ Pyk 2 induced activation of Src is necessary for phosphorylation of Shc and p130Cas and their association with Grb 2 and Crk , respectively , and for the activation of ERK and JNK cascades . ^^^ Expression of a Grb 2 mutant with a deletion of the amino terminal Src homology 3 domain or the carboxyl terminal tail of Sos strongly reduced Pyk 2 induced ERK activation , with no apparent effect on JNK activity . ^^^ Grb 2 with a deleted carboxyl terminal Src homology 3 domain partially blocked Pyk 2 induced ERK and JNK pathways , whereas expression of dominant interfering mutants of p130Cas or Crk specifically inhibited JNK but not ERK activation by Pyk 2 . ^^^ |
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| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| However , the association of Pyk 2 with paxillin and Grb 2 was not affected by stimulation with MCP 1 . ^^^ Our results indicate that Pyk 2 forms a complex with paxillin , Grb 2 and Lyn in THP 1 cells . ^^^ |
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| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| At 1 min after ECS , the binding of Pyk 2 to Src and Grb 2 , and of Grb 2 to Ras increased . ^^^ These results suggested that ECS activates Pyk 2 , which then transmits the signal to MAPK cascade via Src , Grb 2 , and Ras in the rat hippocampus . . ^^^ |
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| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| LPC stimulated the association of Shc with PYK 2 , Shc tyrosine phosphorylation , and Grb 2 binding to Shc and induced Ras activation . ^^^ |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| We have investigated the regulation of Pyk 2 in human umbilical vein endothelial cells in response to GPCAs and show that ( 1 ) thrombin , a PAR 1 peptide , and histamine cause rapid concentration and time dependent phosphorylation on tyrosines 402 ( Src kinase binding site ) , 881 ( Grb 2 binding site ) , and 580 ( an autophosphorylation site ) , ( 2 ) thrombin stimulated phosphorylation is dependent on intracellular calcium and independent of PKC and PI 3 kinase , and ( 3 ) inhibition of Src kinases has no significant effect on thrombin stimulated phosphorylation , implying that tyrosine phosphorylation of Pyk 2 is independent of Src binding . . ^^^ |
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| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| Moreover , in response to thrombin stimulation Pyk 2 formed a complex with Src tyrosine kinase and adapter protein growth factor receptor bound protein 2 ( Grb 2 ) , which could be coprecipitated . ^^^ From these data we conclude that PAR 1 uses Ca2+ and PKC dependent Pyk 2 to activate Src , thereby leading to ERK1 / 2 activation , which predominantly recruits Grb 2 in rat astrocytes . . ^^^ |
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| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| RESULTS : The SH 2 containing adapter protein Crk underwent glucose induced association with p130Cas , while other SH 2 containing proteins such as grb 2 , PI 3 kinase , Shp 2 , paxillin , and pyk 2 did not . ^^^ |
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| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| In addition to interactions with multiple signaling adaptors Grb 2 , SHC , SCK , and NSP 2 , EGF receptors in HN 5 cells were shown to form direct or indirect physical interactions with additional kinases including ACK 1 , focal adhesion kinase ( FAK ) , Pyk 2 , Yes , EphA 2 , and EphB 4 . ^^^ |
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| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| It involves translocation ( i . e . activation ) of PKCalpha from the cytosol to the membrane and / or the Triton 10 100 insoluble subcellular fractions , with recruitment into a complex with alphaVbeta 3 integrin , growth factor receptor bound protein ( Grb 2 ) , focal adhesion kinase ( FAK ) in CHO cells and proline rich tyrosine kinase ( PYK 2 ) in osteoclasts . ^^^ |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|