| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.59159596 |
| Molecular cloning of SLP 76 , a 76 kDa tyrosine phosphoprotein associated with Grb 2 in T cells . 0.59159596^^^ |
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| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :1.0687048 |
| Here we demonstrate formally that Grb 2 associates with unphosphorylated SLP 76 and map the Grb 2 binding site on SLP 76 undergoes rapid tyrosine phosphorylation and associates with tyrosine phosphoproteins of 36 , 62 , and 130 kD . 1.0687048^^^ |
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| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.89645125 |
| As SLP 76 interacts with both Grb 2 and phospholipase C gamma 1 , these data indicate that phosphorylation of SLP 76 by ZAP 70 provides an important functional link between the T cell receptor and activation of ras and calcium pathways . . 0.89645125^^^ |
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| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.50856853 |
| Furthermore , in vitro experiments show that after antigen stimulation , phosphorylated p95vav SLP 76 can bind to Grb 2 in a complex that contains pp36 / 38 and pp 116 proteins . 0.50856853^^^ |
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| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.60538019 |
| We provide evidence that SLP 76 , Cbl , and Shc are tyrosine phosphorylated on FcgammaRI receptor stimulation and are associated with the adapter protein Grb 2 in gamma interferon differentiated U 937 cells ( U937IF ) . 0.60538019^^^ |
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| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.52468917 |
| SLP 76 , a recently cloned Grb 2 associated protein , was strongly tyrosine phosphorylated after Fc gammaRI stimulation and was associated with both Cbl and Grb 2 . 0.52468917^^^ |
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| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylation of p 38 and p 63 is also observed in platelets stimulated by the tyrosine kinase linked receptor agonist collagen and by the G protein coupled receptor agonist thrombin , although phosphorylation of SLP 76 is only observed in collagen stimulated platelets . p 38 and p 63 may provide a docking site for Grb 2 , thereby linking Grb 2 SH3 binding proteins SOS 1 , SLP 76 , and p 120 to downstream signalling events . . ^^^ |
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| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.0 |
| We show further that the 76 kDa protein is SLP 76 , a hematopoietic cell specific protein first identified as a Grb 2 binding protein in T cells . ^^^ |
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| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.0 |
| Consistent with this observation , pp 55 selectively binds to isolated SH 2 domains of Lck , Lyn , Src , and Fyn but not to the SH 2 domains of ZAP 70 , Syk , Shc , SLP 76 , Grb 2 , phosphatidylinositol 3 kinase , and c abl in vitro . ^^^ |
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| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.0 |
| Grf 40 , A novel Grb 2 family member , is involved in T cell signaling through interaction with SLP 76 and LAT . ^^^ Grf 40 binds to the SH 2 domain containing leukocyte protein of 76 kD ( SLP 76 ) via its SH 3 domain more tightly than Grb 2 . ^^^ Our data suggest that Grf 40 is an adaptor molecule involved in TCR mediated signaling through a more efficient interaction than Grb 2 with SLP 76 and LAT . . ^^^ |
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| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.0 |
| Our results provide the first evidence that FcalphaR mediated signals modulate a multimolecular adaptor protein complex containing Grb 2 , Shc , SHIP , CrkL , Cbl , and SLP 76 . ^^^ |
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| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.0 |
| Subsequent analysis revealed that Src and Syk / ZAP 70 tyrosine kinases and the adaptor proteins LAT , SLP 76 , BLNK , Grb 2 , and Grap are involved in HPK 1 activation . ^^^ |
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| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.0 |
| The PXXXR motif is required but not sufficient for Grb 2 binding , whereas an extended motif , PX3RX2KPX7PLD , conserved in Gab proteins as well as the Grb2 / Gads docking protein , Slp 76 , efficiently competes binding of Grb 2 or Gads adapter proteins . ^^^ |
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| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.0 |
| The transmembrane adapter LAT coprecipitates with SLP 76 and PLCgamma 2 , as well as with a number of other adapter proteins , some of which have not been previously described in platelets , including Cbl , Grb 2 , Gads , and SKAP HOM . ^^^ There was no detectable association between Grb 2 and SLP 76 in control or stimulated cells , suggesting that the interaction of LAT with Grb 2 is present in a separate complex to that of LAT Gads SLP 76 . ^^^ |
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| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.0 |
| Following ligation of CD 2 on the surface of the Jurkat T cell line and human purified T cells , LAT was tyrosine phosphorylated and shown to associate in vivo with a number of other tyrosine phosphorylated proteins including PLCgamma 1 , Grb 2 , and SLP 76 . ^^^ |
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| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.0 |
| Involvement of LAT , Gads , and Grb 2 in compartmentation of SLP 76 to the plasma membrane . ^^^ Moreover , we demonstrate that addition of both linker for activation of T cells ( LAT ) and Grb 2 related adaptor downstream of Shc ( Gads ) to SLP 76 allow SLP 76 to be recruited into GEMs , whereby the BCR function is reconstituted . ^^^ In contrast to SLP 76 , BLNK did not require Grb 2 families for its recruitment to GEMs . ^^^ |
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| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.0 |
| Western blot analysis demonstrated that neither pp 70 nor pp 68 represented the recently described linker protein SLP 76 , which binds Grb 2 , PLC , and Vav in T cells after T cell receptor activation . ^^^ |
|
| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.0 |
| This protein , named SLP 65 , is part of a signaling complex involving Grb 2 and Vav and shows homology to SLP 76 , a signaling element of the T cell receptor . ^^^ |
|
| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.0 |
| GrpL , a Grb 2 related adaptor protein , interacts with SLP 76 to regulate nuclear factor of activated T cell activation . ^^^ In contrast , Grb 2 can be coimmunoprecipitated with Sos 1 and Sos 2 but not with SLP 76 . ^^^ |
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| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of LAT creates binding sites for the Src homology 2 ( SH 2 ) domains of other proteins , including PLC gamma 1 , Grb 2 , Gads , Grap , 3BP2 , and Shb , and indirectly binds SOS , c Cbl , Vav , SLP 76 , and Itk . ^^^ |
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| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.0 |
| Gads , a member of the Grb 2 family of SH 2 and SH 3 domain containing adaptors , mediates the formation of a complex between LAT and SLP 76 that is essential for signal propagation from the TCR . ^^^ Here we examine the binding specificity of the Gads and Grb 2 SH3 domains using peptide arrays and find that a nonproline based R 10 10 K motif found in SLP 76 binds to the Gads carboxy terminal SH 3 domain with high affinity ( K ( D ) = 240 + / 45 nM ) . ^^^ |
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| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.0 |
| Within seconds of contacting coverslips coated with stimulatory antibodies , T cells developed small , dynamically regulated clusters which were enriched in the TCR , phosphotyrosine , ZAP 70 , LAT , Grb 2 , Gads , and SLP 76 , excluded the lipid raft marker enhanced yellow fluorescent protein GPI , and were competent to induce calcium elevations . ^^^ |
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| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.0 |
| HPK 1 binds many adaptor proteins including members of the Grb 2 family , Nck family , Crk family , SLP 76 family , and actin binding adaptors like HIP 55 . ^^^ The HPK 1 association with Crk , CrkL , and HIP 55 mediate HPK 1 dependent c Jun N terminal kinase ( JNK ) activation , while the association of HPK 1 with SLP 76 , Gads , CrkL , Grb 2 , and Grap affect T and B cell dependent gene transcription . ^^^ |
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| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.0 |
| Intriguingly mutation of critical residues for the three sites not only affected binding of SLP 76 to the PLC gamma 1 SH3 domain but also to the Grb 2 C terminal SH 3 domain , indicating that the binding sites in SLP 76 for the two SH 3 domains are overlapped . ^^^ |
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| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.0 |
| Interactions with SH 2 domain adapters [ growth factor receptor binding protein 2 ( Grb 2 ) , Cbl , Slp 76 ] and SH 3 domain adapters ( HS 1 , cortactin , CD2BP3 ) were attenuated by inhibition of Kit kinase activity . ^^^ |
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| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13094 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|