| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :1.1483166 |
| Among upstream signaling molecules of ERK , Shc was constitutively associated with Grb 2 and was not tyrosine phosphorylated by GM CSF and FMLP , and Sos 1 and c Raf 1 were not phosphorylated by GM CSF , IL 3 , TNF , and FMLP in monocytes , whereas all these signaling molecules were affected and / or utilized by GM CSF in MO7e cells . 1.1483166^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Although overexpression of IRS 1 increased IRS 1 binding to Grb 2 , ERK 1 / ERK 2 activation was not rescued . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| We have used activation of the mitogen activated protein kinase Erk 1 as a model system with which to examine whether various dominant negative SH2 / SH3 adapters ( Grb 2 , Crk , and Nck ) could block signaling pathways leading to Erk activation . ^^^ Activation of Erk 1 by oncogenic Abl was effectively inhibited by Grb 2 with mutations in either its SH 2 or SH 3 domain or by Crk 1 with an SH 3 domain mutation . ^^^ These results suggest that both Crk and Grb 2 may contribute to the activation of Erk by oncogenic Abl , whereas Nck is unlikely to participate in this pathway . ^^^ In contrast to results with Abl , Erk activation by EGF was strongly inhibited only by Grb 2 mutants ; Crk and Nck mutants had little or no effect . ^^^ This finding suggests that Grb 2 is the only adapter involved in the activation of Erk by EGF . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Although expression of the mitogen activated protein kinase specific phosphatase ( MKP 1 ) completely inhibited the insulin stimulation of ERK activity both in vitro and in vivo , SOS phosphorylation and the dissociation of the Grb 2 SOS complex were unaffected . ^^^ Together , these data demonstrate that at least one of the kinases responsible for SOS phosphorylation and functional dissociation of the Grb 2 SOS complex is an ERK independent but MEK dependent insulin stimulated protein kinase . . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| SOS phosphorylation and disassociation of the Grb 2 SOS complex by the ERK and JNK signaling pathways . ^^^ These data demonstrate that activation of the ERK pathway , but not JNK , is responsible for the feedback phosphorylation and disassociation of the Grb 2 SOS complex . . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Insulin stimulates the Ras / Raf / MEK / ERK pathway leading to feedback phosphorylation of the Ras guanylnucleotide exchange protein SOS and dissociation of Grb 2 from SOS . ^^^ Even though epidermal growth factor ( EGF ) also stimulates ERK activity and phosphorylation of SOS similar to insulin , EGF induces a dissociation of the Grb 2 SOS complex from Shc . ^^^ Although EGF stimulation of the mutant EGF receptor activates ERK and phosphorylation of both Shc and SOS , it fails to directly associate with either Shc or Grb 2 . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| The increase observed in the phosphotyrosine content of the 52 kDa Shc protein , accompanied by an increased amount of co immunoprecipitated Grb 2 , and the activation of Raf 1 kinase provide evidence of the involvement of a Ras Raf dependent pathway , with a time course that is similar to that of ERK activation . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Activation of ERK 1 and 2 may involve the Src homology 2 containing proteins Shc and Grb 2 . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Ligation of alpha6beta4 in primary human keratinocytes caused tyrosine phosphorylation of Shc , recruitment of Grb 2 , activation of Ras and stimulation of the MAP kinases Erk and Jnk . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Transfection of cDNA constructs encoding inactive mutant phosphoinositide 3 ' kinase , Grb 2 , Sos , Ras , and Raf molecules were successful in attenuating ERK , but had essentially no effect upon NHE activation . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Interestingly , the mutant protein still dissociates from Grb 2 following insulin stimulation , suggesting that ERK is not the kinase responsible for regulating the stability of the Grb2 . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Growth factor derived mitogenic signals from the cell surface are transmitted to the nucleus via receptor tyrosine kinases ( RTKs ) , the adaptor proteins Shc and Grb 2 , and a Ras dependent protein kinase cascade that activates the extracellular signal regulated kinase ( ERK ) subfamily of mitogen activated protein kinases . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Cholesterol depleted caveolae had a reduced amount of several key protein components of the MAP kinase complex , including Ras , Grb 2 , Erk 2 , and Src . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| The arsenite induced tyrosine phosphorylation of Shc , enhancement of Shc and Grb 2 interactions , and activation of ERK were all drastically reduced by treatment of cells with either the general growth factor receptor poison suramin or the EGFR selective inhibitor tyrphostin AG 1478 . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Here we demonstrate that the BCR induced ERK activation is reduced by loss of Grb 2 or expression of a dominant negative form of Ras , RasN 17 , whereas this response is not affected by loss of Shc . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Although a selective EGFR kinase inhibitor completely abolished Ang 2 induced recruitment of Grb 2 to EGFR and markedly attenuated Ang 2 induced ERK activation , it had no effect on Ang 2 induced PYK 2 tyrosine phosphorylation or its association with c Src and Grb 2 . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| In this report , we provide evidence that the protein tyrosine kinase Src and adaptor proteins Grb 2 , Crk , and p130Cas act as downstream mediators of Pyk 2 leading to the activation of extracellular signal regulated kinase ( ERK ) and c Jun amino terminal kinase ( JNK ) . ^^^ Pyk 2 induced activation of Src is necessary for phosphorylation of Shc and p130Cas and their association with Grb 2 and Crk , respectively , and for the activation of ERK and JNK cascades . ^^^ Expression of a Grb 2 mutant with a deletion of the amino terminal Src homology 3 domain or the carboxyl terminal tail of Sos strongly reduced Pyk 2 induced ERK activation , with no apparent effect on JNK activity . ^^^ Grb 2 with a deleted carboxyl terminal Src homology 3 domain partially blocked Pyk 2 induced ERK and JNK pathways , whereas expression of dominant interfering mutants of p130Cas or Crk specifically inhibited JNK but not ERK activation by Pyk 2 . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| The paradigm for activation of Ras and extracellular signal regulated kinase ( ERK ) / mitogen activated protein ( MAP ) kinase by extracellular stimuli via tyrosine kinases , Shc , Grb 2 , and Sos does not encompass an obvious role for phosphoinositide ( PI ) 3 kinase , and yet inhibitors of this lipid kinase family have been shown to block the ERK / MAP kinase signalling pathway under certain circumstances . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| In addition , ZAS induced the tyrosine phosphorylation of the SHC proteins and their association with GRB 2 , suggesting a role for this complex in the activation of the ERK pathway . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| To examine the relationship between the Grb 2 SOS complex and Ras activation , we observed that insulin stimulation results in a rapid but transient activation of Ras and the extracellular signal regulated kinase ( ERK ) followed by dissociation of the Grb 2 SOS complex . ^^^ Although treatment with the phorbol myristate acetate resulted in ERK activation and complete dissociation of the Grb 2 SOS complex , there was no effect on subsequent insulin stimulated Ras activation . ^^^ Together , these data demonstrate the presence of a pathway independent of the Grb 2 SOS complex that can lead to Ras activation but that the desensitization of Raf accounts for the homologous desensitization of ERK . . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Two adaptor molecules , Grb 2 and Shc , have been implicated in the extracellular signal regulated kinase ( ERK ) activation by receptor tyrosine kinases such as the epidermal growth factor receptor ( EGFR ) . ^^^ Here we show that the EGF mediated ERK activation is abolished by loss of Grb 2 , whereas this response is not affected by loss of Shc . ^^^ These findings strongly support distinct roles for Grb 2 and Shc in controlling ERK and JNK activation after EGF stimulation . . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| The tyrphostin AG 1478 , known to inhibit EGF receptor phosphorylation , also inhibited the angiotensin 2 and EGF induced activation of ERK , the phosphorylation of the EGF receptor , and the subsequent association of Shc and Grb 2 . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| In addition to requirements for PI3K , PDK 1 , and PKC zeta , we found that a tyrosine kinase ( presumably the insulin receptor ) , the SH 2 domain of GRB 2 , SOS , RAS , RAF , and MEK 1 were required for insulin effects on ERK in the rat adipocyte . ^^^ Our findings therefore suggested that PDK 1 and PKC zeta serve as a downstream effectors of PI3K , and act in conjunction with GRB 2 , SOS , RAS , and RAF , to activate MEK and ERK during insulin action in rat adipocytes . . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| In contrast , the phosphorylation of LAT and enhanced association with Grb 2 led to activation of extracellular signal regulated kinase ( ERK ) mitogen activated protein kinase . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| In cardiac fibroblasts , angiotensin 2 activated ERK via the G ( beta ) gamma subunit of Gi , Src , Shc , Grb 2 , and Ras , whereas Gq and protein kinase C were critical in cardiomyocytes . . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Interestingly , priming of chemoattractant induced ERK activation is accompanied by an increase in association of tyrosine phosphorylated proteins with the adapter protein Grb 2 . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| The enhanced activation of EGFR by the tethered form of TGFalpha was reflected by higher activation of Grb 2 , SHC and Erk downstream mediators of EGF receptor signaling . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Tyr ( 985 ) mediated recruitment of SHP 2 does not alter tyrosine phosphorylation of Jak 2 or STAT 3 but results in GRB 2 binding to tyrosine phosphorylated SHP 2 and is required for the majority of ERK activation during LRb signaling . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Recruitment to activated tyrosine kinase growth factor receptors of Grb 2 and p 21 ( ras ) leads to downstream activation of the kinases Raf , MAPK / Erk kinase ( Mek ) and , subsequently , extracellular signal regulated kinase ( Erk ) . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Mutation of individual Grb 2 binding sites had no functional effect , but mutation of two or three of these sites , in combination , also affected Erk and NF AT activation . . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Analysis of these cells indicates that FAK is not necessary for efficient tyrosine phosphorylation of Shc , association of Shc with Grb 2 , and activation of ERK in response to matrix adhesion . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Mutation of Tyr 1096 ( Y1096F ) , a binding site for the adaptor Grb 2 , had no effect on Erk activation by GDNF . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Presently , we found that glucose and certain sugars rapidly activated extracellular signal regulated kinase ( ERK ) by a mechanism that was : ( a ) independent of glucose uptake / metabolism and protein kinase C but nevertheless cytochalasin B inhibitable ; ( b ) dependent upon proline rich tyrosine kinase 2 ( PYK 2 ) , GRB 2 , SOS , RAS , RAF , and MEK 1 ; and ( c ) amplified by overexpression of the Glut 1 , but not Glut 2 , Glut 3 , or Glut 4 , glucose transporter . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| We show here that decreased expression of the adaptor protein Grb 2 in Grb2+ / mice weakens TCR induced c Jun N terminal kinase ( JNK ) and p 38 , but not extracellular signal regulated kinase ( ERK ) , activation . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Mutagenesis of SNT 1 ( IRS ) CX identified three classes of effector motifs within SNT critical for both sustained ERK activation and neuronal differentiation : 1 ) four phosphotyrosine motifs that mediate recruitment of Grb 2 , 2 ) two phosphotyrosine motifs that mediate recruitment of Shp 2 , and 3 ) a C terminal motif that functions by helping to recruit Sos . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| DOC 2 / DAB2 mediated inhibition of ERK phosphorylation via binding to Grb 2 . ^^^ Such elevated binding interrupts the binding between SOS and Grb 2 , which consequently suppresses downstream ERK phosphorylation . ^^^ Reduced ERK phosphorylation was restored when the binding between DOC 2 / DAB2 and Grb 2 was interrupted by a specific peptide or by increasing the expression of Grb 2 . ^^^ We conclude that DOC 2 / DAB2 , a potent negative regulator , can suppress ERK activation by interrupting the binding between Grb 2 and SOS that is elicited by peptide growth factors . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Full reconstitution of Erk requires two additional tyrosine residues ( Tyr ( 110 ) and Tyr ( 226 ) ) , both of which have the Grb 2 binding motif YXN . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Biochemical analyses correlated Erk activation by Grb 2 FAT with its stimulation of cell cycle progression . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Presently , we found that glucose acutely activated PKC zeta / lambda in rat adipocytes and rat skeletal muscle preparations by a mechanism that was independent of phosphatidylinositol 3 kinase but , interestingly , dependent on the apparently sequential activation of the dantrolene sensitive , nonreceptor proline rich tyrosine kinase 2 ; components of the extracellular signal regulated kinase ( ERK ) pathway , including , GRB 2 , SOS , RAS , RAF , MEK 1 and ERK1 / 2 ; and , most interestingly , phospholipase D , thus yielding increases in phosphatidic acid , a known activator of PKC zeta / lambda . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Many downstream effectors of Bcr Abl have been described , including activation of the Grb 2 SoS Ras MAP kinase ( Erk ) pathway . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| PD 98059 , a specific inhibitor of the ERK activating kinase MEK 1 , abolished the TNF alpha induced ERK phosphorylation and osteoclast survival , and in these responses the involvement of Grb 2 and ceramide was observed . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| In vivo expression of a Grb 2 mutant where Tyr 209 was changed to phenylalanine enhanced BCR / ABL induced ERK activation and fibroblast transformation , and potentiated and prolonged Grb 2 mediated activation of Ras , mitogen activated protein kinase and c Jun N terminal kinase in response to EGF stimulation . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Immunoblotting indicated the presence of Shc , Grb 2 , Ras ( p 21 ) , Raf and ERK 1 and 2 ( ERK1 / 2 ) in spermatozoa . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Pretreatment of AEC with Src homology 3 binding peptide , which blocks the interaction between Grb 2 and Sos , did not prevent DA activation of ERK . ^^^ Together , the data suggest that DA activated MAPK / ERK via Ras , Raf 1 kinase , and DAG dependent PKC isoenzymes , but , importantly and contrary to the classical model , this pathway did not involve the Grb 2 Sos complex formation . . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| We also show that the interaction of EphA 2 with GRB 2 is indirect and mediated by SHC and that this complex is necessary for EphA 2 mediated activation of ERK kinases . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| A tyrosine phosphorylated octapeptide derived from mouse Spry 2 inhibits Grb 2 from binding FRS 2 , Shp 2 or mouse Spry 2 in vitro and blocks activation of the extracellular signal regulated kinase ( ERK ) in cells stimulated by growth factor . ^^^ A non phosphorylated Spry mutant can not bind Grb 2 and acts as a dominant negative , inducing prolonged activation of ERK in response to FGF and promoting the FGF induced outgrowth of neurites in PC 12 cells . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| An EphB 2 mutant that retained juxtamembrane ( JM ) RasGAP binding sites but incorporated a Grb 2 binding motif at an alternate RasGAP binding site within the kinase domain had little effect on basal Erk MAPK activation . ^^^ In contrast , elimination of all RasGAP binding sites , accompanied by the addition of a Grb 2 binding site within the kinase domain , led to an increase in phospho Erk levels in NG 108 cells following ephrin B 1 stimulation . ^^^ These data suggest that EphB 2 can be designed to repress , stabilize , or activate the Ras Erk MAPK pathway by the manipulation of RasGAP and Grb 2 SH2 domain binding sites and support the notion that Erk MAPK regulation plays a significant role in axon guidance . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| In addition , LH induced IRS 1 / phosphoinositol 3 kinase and Shc / growth factor receptor binding protein 2 ( Grb 2 ) associations and downstream AKT ( protein kinase B , homologous to 5 AKT ) serine phosphorylation and ERK tyrosine phosphorylation , respectively . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| These results suggest that morphine attenuates IR signaling to Akt by disrupting the IRS 1 p 85 interaction but inhibits signaling to ERK by disruption of the complex among the IR , Shc , and Grb 2 . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Moreover , because Y580F mutants retain nearly wild type Grb 2 binding ability , yet exhibit defective PDGF evoked ERK activation , our results show that the association of Grb 2 with Shp 2 is not sufficient for promoting full ERK activation in response to these growth factors , thereby arguing strongly against the `` Grb 2 adapter ' ' model of Shp 2 action . . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Here we demonstrate that activated EphB 1 recruits the adaptor proteins Grb 2 and p52Shc and promotes p52Shc and c Src tyrosine phosphorylation as well as MAPK / extracellular signal regulated kinase ( ERK ) activation . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Effector proteins that are key mediators of the ERK and PI3K pathways , namely Grb 2 , the tyrosine phosphatase , SHP 2 and PI3K , interact with the two phosphotyrosines found in the bidentate motif in the carboxy terminal region of 5 SEA . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Finally , ANXA 1 regulates the ERK pathway at a proximal location , by SH 2 domain independent association with the adapter protein Grb 2 . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Furthermore , progestin treatment prior to IGF 1 stimulation resulted in higher tyrosine phosphorylated IRS 2 levels , increased binding of IRS 2 to Grb 2 and the PI3K regulatory subunit p 85 , and correspondingly enhanced ERK and Akt activation , as compared with IGF 1 only conditions . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| In this present study using the small interfering RNA technique , we have found that ShcA adapter proteins play a rather active role in CSR induced Erk activation , contrary to their mostly redundant role in other signaling pathways , e . g . growth factor induced Erk activation , where Grb 2 can bind directly to the receptor tyrosine kinase and activate Erk in the absence of ShcA . ^^^ In a quest for molecules upstream of ShcA in this signaling , we found that CSR induced ShcA tyrosine phosphorylation , its association with Grb 2 , Erk activation , and uPA gene expression were all dependent on Rho kinase , p 38 mitogen activated protein kinase , and Src . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Immunocytochemistry on kidney sections using specific antibodies against TGF alpha , HB EGF , EGFr , IGF 1Rbeta , Grb 2 , and phospho p44 / 42 MAP kinase ( ERK1 / 2 ) revealed a significantly higher staining of these molecules 3 to 72 hours after dosing , indicating up regulation of the ERK pathway . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Incubation of 1 LN cells with alpha2M * induced Grb 2 , shc , sos and Raf 1 expression , as well as phosphorylation of MEK 1 / 2 , ERK 1 / 2 , p 38 MAPK and JNK . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| The morphogenetic activity of CagA also required Erk MAPK but was independent of Ras or Grb 2 . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| ERK1 / 2 and the important ERK pathway upstream small adaptor protein , Grb 2 ( growth factor receptor bound protein 2 ) , were also enriched in caveolae ( 2 . 3 and 8 . 3 fold , respectively ) , but STAT 5 was barely detected in the same fraction . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| In contrast , grb 2 heterozygosity did not alter tumorigenesis in either pten+ / or T cell specific pten / mice . grb 2 / hypomorph murine embryonic fibroblasts ( MEFs ) show decreased activation of both PKB and Erk upon stimulation with epidermal growth factor , whereas grb 2 / hypomorph ; pten+ / MEFs activate PKB but not Erk normally . ^^^ Activation of both PKB and Erk as well as survival in low serum containing media are all rescued by reexpression of Grb 2 containing mutations within the N terminal Src homology 3 ( SH 3 ) domain , but not by C terminal SH 3 domain mutants . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| After FGF stimulation , tyrosine phosphorylated FRS2alpha recruits four molecules of the adaptor protein Grb 2 and two molecules of the protein tyrosine phosphatase Shp 2 , resulting in activation of the Ras / extracellular signal regulated kinase ( ERK ) and phosphatidylinositol 3 kinase / Akt signaling pathways . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Prolidase activity and collagen biosynthesis are supposed to be regulated by beta ( 1 ) integrins , which initiate a signaling pathway in which several kinases and intracellular proteins are involved , including focal adhesion kinase pp 125 ( FAK ) ( FAK ) , Src , Shc , growth factor receptor bound protein 2 ( Grb 2 ) , son of sevenless protein ( SOS ) , Ras , Raf and mitogen activated protein kinases ( MAPK ) , extracellular signal regulated kinase 1 ( ERK ( 1 ) ) and kinase 2 ( ERK ( 2 ) ) . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| PP 2 and RGD peptide also inhibited high pressure induced binding of FAK with the effector Grb 2 and blocked activation of extracellular regulated kinase ( ERK ) 1 / 2 in vessels at 150 mm Hg . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| In addition , similar results for Cav3 . 1 activation were also shown in the case of Sos 1 , Grb 2 , and Shc , which means that Cav3 . 1 activation triggers p 21 ( ras ) and that this signal is transferred to ERK by Sos 1 , Grb 2 , and Shc . . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Direct recruitment of CRK and GRB 2 to VEGFR 3 induces proliferation , migration , and survival of endothelial cells through the activation of ERK , AKT , and JNK pathways . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| Application of exogenous H2O2 caused increased tyrosine phosphorylation and Grb 2 binding to IRS 1 in SK N BE ( 2 ) cells , while exposure to an H2O2 scavenger ( N acetylcysteine ) or to a phophatidylinositol 3 kinase inhibitor ( wortmannin ) , and expression of a dominant negative Rac 1 , decreased the activation of ERK in insulin stimulated PC 12 cells . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| The E76K mutation in the N terminal Src homology 2 domain increased interactions of mutant SHP 2 with Grb 2 , Gab 2 , and p 85 , leading to hyperactivation of IL 3 induced Erk and phosphatidylinositol 3 kinase ( PI3K ) pathways . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| The expression of beta ( 1 ) integrin receptor , as well as Sos 1 and phosphorylated MAPK , ERK ( 1 ) and ERK ( 2 ) but not FAK , Shc , and Grb 2 was significantly decreased in cells incubated for 24h with 20 microM AB 4 compared to the control , not treated cells , whereas in the same conditions melphalan did not evoke any changes in expression of all these signaling proteins , as shown by Western immunoblot analysis . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| A novel redox based switch : LMW PTP oxidation enhances Grb 2 binding and leads to ERK activation . ^^^ The redox dependent enhancement of Grb 2 recruitment to LMW PTP ultimately leads to an improvement of ERK activation , likely triggering a prosurvival signal against the oxidant environment . . ^^^ |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29323 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|