| Interacting proteins: P08247 and P62993 |
Pubmed |
SVM Score :0.54334054 |
| Direct binding between Syp and Grb 2 was also observed in vitro . 0.54334054^^^ |
|
| Interacting proteins: P08247 and P62993 |
Pubmed |
SVM Score :0.60941481 |
| Furthermore , Syp became marginally phosphorylated on tyrosine upon c kit receptor activation , and tyrosine phosphorylated Syp was found to be complexed with Grb 2 in steel factor stimulated M07e cells . 0.60941481^^^ Direct binding between Syp and Grb 2 was also observed in vitro . 0.54334054^^^ |
|
| Interacting proteins: P08247 and P62993 |
Pubmed |
SVM Score :1.5733676 |
| Furthermore , both GRB 2 and Sos coimmunoprecipitate with Syp from lysates of PDGF stimulated cells , and GRB 2 binds directly to tyrosine phosphorylated Syp in vitro . 1.5733676^^^ |
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| Interacting proteins: P08247 and P62993 |
Pubmed |
SVM Score :0.0 |
| Furthermore , p210bcr abl , Syp , and Grb 2 formed stable complexes in BCR ABL expressing cells . ^^^ |
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| Interacting proteins: P08247 and P62993 |
Pubmed |
SVM Score :0.0 |
| By contrast , the SH 2 domain in GRB 2 and the amino terminal SH 2 domain in SHPTP 2 ( Syp ) specifically bind to Tyr ( P ) 895 and Tyr ( P ) 1172 , respectively . ^^^ These results confirm the p 85 alpha recognizes YMXM motifs and suggest that GRB 2 prefers a phosphorylated YVNI motif , whereas SHPTP 2 ( Syp ) binds to a phosphorylated YIDL motif . ^^^ |
|
| Interacting proteins: P08247 and P62993 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylation of IRS 1 causes it to associate with the src homology 2 ( SH 2 ) domains of at least four other proteins : phosphatidylinositol 3 ' kinase ( PI3K ) , growth factor receptor bound protein 2 ( GRB 2 ) , Nck , and Syp . ^^^ |
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| Interacting proteins: P08247 and P62993 |
Pubmed |
SVM Score :0.0 |
| Significantly , all the insulin induced tyrosine phosphorylation sites identified in rat IRS 1 , including those responsible for binding to the Src homology domains of phosphatidylinositol ( PI ) 3 kinase , Syp and Grb 2 , are conserved in XIRS L . ^^^ |
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| Interacting proteins: P08247 and P62993 |
Pubmed |
SVM Score :0.0 |
| Grb 2 immunoprecipitation from IRS 1 transfected and insulin treated cells demonstrated an increased coimmunoprecipitation of Syp and the p 85 regulatory subunit of the phosphatidylinositol 3 kinase . ^^^ Similarly , cell extracts immunoprecipitated with a p 85 antibody displayed an increased co immunoprecipitation of Syp and Grb 2 . ^^^ |
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| Interacting proteins: P08247 and P62993 |
Pubmed |
SVM Score :0.0 |
| Insulin promotes the formation of a complex between tyrosine phosphorylated IRS 1 and several proteins including phosphoinositide ( PI ) 3 kinase , a heterodimer consisting of regulatory 85 kDa ( p 85 ) and catalytic 110 kDa ( p 110 ) subunits , GRB 2 and Syp via the Src homology region 2 ( SH 2 ) domains . ^^^ |
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| Interacting proteins: P08247 and P62993 |
Pubmed |
SVM Score :0.0 |
| Anti Shc antibodies co immunoprecipitated tyrosine phosphorylated 145 kD proteins ( p 145 ) , whereas GRB 2 immunoprecipitates contained phosphorylated Shc , Syp , and proteins of 145 and 90 kD ( p 90 ) . ^^^ |
|
| Interacting proteins: P08247 and P62993 |
Pubmed |
SVM Score :0.0 |
| We have recently shown that the SH 2 containing tyrosine phosphatase , Syp , binds directly to both the tyrosine phosphorylated form of the Epo receptor ( EpoR ) and to Grb 2 after Epo stimulation of M07e cells engineered to express high levels of human EpoRs ( T . ^^^ |
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| Interacting proteins: P08247 and P62993 |
Pubmed |
SVM Score :0.0 |
| The inhibitor exhibited specificity for the PDGF type beta receptor , and consistently co purified with activated p 21 ras , with Syp / PTP 2 , and with Grb 2 . ^^^ These results indicate that activated p 21 ras either interacts directly with the PDGF type beta receptor to inhibit autokinase activity , or complexes with different molecules such as Syp and / or Grb 2 at the cell membrane to act on another effector which then inhibits PDGF type beta receptor function . . ^^^ |
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| Interacting proteins: P08247 and P62993 |
Pubmed |
SVM Score :0.0 |
| Neoplastic transformation induced by insulin receptor substrate 1 overexpression requires an interaction with both Grb 2 and Syp signaling molecules . ^^^ This study examines the role of two SH 2 containing molecules , namely the Grb 2 adapter and Syp tyrosine phosphatase proteins as important components of the cellular transforming activity of IRS 1 . ^^^ Mutations of tyrosine 897 in the YVNI motif ( Y897F ) and of tyrosine 1180 in the YIDL motif ( Y1180F ) reduced the intracellular interaction of IRS 1 with Grb 2 and Syp proteins , respectively . ^^^ These results suggest that IRS 1 induced cellular transformation requires an interaction with both Grb 2 and Syp signal transduction molecules since neither interaction alone appears to be required , and this event subsequently leads to activation of the MAPKK / MAPK cascade . . ^^^ |
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| Interacting proteins: P08247 and P62993 |
Pubmed |
SVM Score :0.0 |
| The Src homology 2 ( SH 2 ) and SH 3 domain containing adaptor protein GRB 2 and the SH 2 domain containing protein tyrosine phosphatase 1D ( PTP1D , also called SHPTP 2 , PTP2C , SHPTP 3 , Syp , or SHP 2 ) function as positive mediators of growth factor induced mitogenesis . ^^^ |
|
| Interacting proteins: P08247 and P62993 |
Pubmed |
SVM Score :0.0 |
| A 60 kilodalton protein in rat hepatoma cells overexpressing insulin receptor was tyrosine phosphorylated and associated with Syp , phophatidylinositol 3 kinase , and Grb 2 in an insulin dependent manner . ^^^ Grb 2 was also observed in the anti Syp immunoprecipitates . ^^^ Studies in rat liver , muscle , and adipose tissue identified insulin dependent association of Syp , Grb 2 , and p 85 with tyrosine phosphorylated p 60 in adipose tissue only . ^^^ |
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| Interacting proteins: P08247 and P62993 |
Pubmed |
SVM Score :0.0 |
| IRS 2 from FD 5 cells stimulated with either IL 4 or insulin bound to glutathione S transferase fusion proteins of the p 85 subunit of phosphoinositol 3 ' kinase , Grb 2 , and Syp , paralleling reported associations of IRS 1 with these molecules and indicating phosphorylation of the corresponding residues on IRS 2 . . ^^^ |
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| Interacting proteins: P08247 and P62993 |
Pubmed |
SVM Score :0.0 |
| Signaling factors that associate with activated erythropoietin ( Epo ) receptor complexes include phospholipase C gamma , phosphatidylinositol 3 kinase , SHIP , Shc , Grb 2 , Cbl , Crk l , HCP , Syp , and STAT 5 . ^^^ |
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| Interacting proteins: P08247 and P62993 |
Pubmed |
SVM Score :0.0 |
| Following PMA induced tyrosine phosphorylation , ErbB 2 and ErbB 3 were able to associate with the SH 2 domains of several signaling proteins including the p85alpha subunit of phosphatidylinositol 3 kinase , Syp , and Grb 2 . ^^^ |
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| Interacting proteins: P08247 and P62993 |
Pubmed |
SVM Score :0.0 |
| Western blot analysis demonstrated that the concentrations of IRS 1 , Shc , GRB 2 , SOS , Syp , PI 3 kinase , MAP kinase and Gi alpha were at least 10 fold higher in cell surface derived , caveolin enriched fraction than in a cell surface derived , caveolin poor fraction ( i . e . , the plasma membranes ) . ^^^ |
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| Interacting proteins: P08247 and P62993 |
Pubmed |
SVM Score :0.0 |
| This downstream signaling is mediated by interactions of specific tyrosyl phosphorylated ( PY ) IRS 1 motifs with SH 2 containing molecules such as growth factor receptor bound protein 2 ( Grb 2 ) and Syp . ^^^ This study explores the roles of the Grb 2 and Syp binding motifs of IRS 1 in relation to the inhibitory effects of ethanol on insulin stimulated DNA synthesis , proliferating cell nuclear antigen ( PCNA ) and glyceraldehyde 3 phosphate dehydrogenase ( GAPDH ) expression , and activation of mitogen activated protein kinase ( MAPK ) , which is known to be essential for cell proliferation . ^^^ NIH3T3 cells were stably transfected with wild type IRS 1 , or IRS 1 mutated at the Grb 2 ( IRS 1deltaGrb2 ) , Syp ( IRS 1deltaSyp ) , or Grb 2 and Syp ( IRS 1deltaGrb2deltaSyp ) binding sites . ^^^ The findings suggest that insulin stimulated DNA synthesis and PCNA expression are mediated through the Syp binding domain , whereas GAPDH expression and MAPK activation are modulated through both the Grb 2 and Syp motifs of IRS 1 . ^^^ |
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| Interacting proteins: P08247 and P62993 |
Pubmed |
SVM Score :0.0 |
| Insulin receptor substrate ( IRS ) proteins are docking proteins that couple growth factor receptors to various effector molecules , including phosphoinositide 3 kinase , Grb 2 , Syp , and Nck . ^^^ |
|
| Interacting proteins: P08247 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08247 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08247 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08247 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08247 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08247 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|