| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.7299803 |
| Phosphorylated LAT binds Grb 2 , PLC gamma 1 , and other signaling molecules . 0.7299803^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.61932795 |
| Association of Grb 2 , Gads , and phospholipase C gamma 1 with phosphorylated LAT tyrosine residues . 0.61932795^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.99918298 |
| Following ligation of CD 2 on the surface of the Jurkat T cell line and human purified T cells , LAT was tyrosine phosphorylated and shown to associate in vivo with a number of other tyrosine phosphorylated proteins including PLCgamma 1 , Grb 2 , and SLP 76 . 0.99918298^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.57890222 |
| After phosphorylation of the cytosolic domain of LAT , multiple signaling molecules such as phospholipase C gamma 1 , Grb 2 , and Gads associate with phosphorylated LAT via their SH 2 domains . 0.57890222^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.5789579 |
| In T cells , the SH 2 domain of GRB 2 binds phosphorylated tyrosines on the adaptor protein LAT and the GRB 2 SH3 domains associate with the proline rich regions of SOS 1 and CBL . 0.5789579^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| A third Grb 2 binding protein ( pp 36 ) was extensively tyrosine phosphophorylated in response to combined CD 3 and CD 28 activation , but not in response to ligation of either receptor alone . cAMP and co ligation of CD 45 affected the receptor activated tyrosine phosphorylation of Grb 2 associated proteins . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| Two of these proteins , pp 36 and pp 116 , bind to the Grb 2 fusion protein with high affinity . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| Activation of T and natural killer ( NK ) cells leads to the tyrosine phosphorylation of pp 36 and to its association with several signaling molecules , including phospholipase Cgamma 1 and Grb 2 . ^^^ When expressed in 293T human fibroblast cells and tyrosine phosphorylated , pp 36 associated with phospholipase Cgamma 1 and Grb 2 . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| CD 16 mediated activation of phosphatidylinositol 3 kinase ( PI 3K ) in human NK cells involves tyrosine phosphorylation of Cbl and its association with Grb 2 , Shc , pp 36 and p 85 PI 3K subunit . ^^^ Overall these results suggest that the formation of a complex containing either Shc or pp 36 associated with Grb 2 , Cbl and the p 85 subunit of Pl 3K is one of the major mechanisms which might couple CD 16 to the Pl 3K pathway in NK cells . . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| In addition to Zap 70 , the 3BP2 SH 2 domain associated in vitro with LAT , Grb 2 , PLCgamma 1 , and Cbl from activated T cell lysates . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| Grf 40 , A novel Grb 2 family member , is involved in T cell signaling through interaction with SLP 76 and LAT . ^^^ Our data suggest that Grf 40 is an adaptor molecule involved in TCR mediated signaling through a more efficient interaction than Grb 2 with SLP 76 and LAT . . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| We have recently investigated the role of the adaptor protein Shb in the early events of T cell signaling and observed that Shb associates with Grb 2 , linker for activation of T cells ( LAT ) and the TCR zeta chain in Jurkat cells . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| Second , the Itk proline rich region binds to Grb 2 and LAT . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| In contrast , the phosphorylation of LAT and enhanced association with Grb 2 led to activation of extracellular signal regulated kinase ( ERK ) mitogen activated protein kinase . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| LAT ( linker for activation of T cells ) is an adaptor molecule , which following its phosphorylation associates with Grb 2 , Gads , PLC gamma 1 , and other signalling molecules . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| Pharmacological interference with ROS production in ConA stimulated thymocytes resulted in a decreased tyrosine phosphorylation of multiple protein species , including a 38 kDa band able to recruit the adapter protein Grb 2 and corresponding to the recently identified transducer LAT ( linker for activation of T cells ) , a molecule involved in linking activated TCR to the production of interleukin 2 and the proliferation of T cells . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| Upon FcepsilonRI cross linking , MIST / Clnk is tyrosine phosphorylated and associates with signaling proteins , phospholipase Cgamma , Vav , Grb 2 and linker for activation of T cells ( LAT ) . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| Co immunoprecipitation experiments revealed a constitutive association of p 36 LAT with both FcgammaRI and FcgammaRIIa immunoprecipitates , and an activation induced association of LAT with PLCgamma 1 , Grb 2 , and the p 85 subunit of phosphatidylinositol 3 kinase . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| Subsequent analysis revealed that Src and Syk / ZAP 70 tyrosine kinases and the adaptor proteins LAT , SLP 76 , BLNK , Grb 2 , and Grap are involved in HPK 1 activation . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| The transmembrane adapter LAT coprecipitates with SLP 76 and PLCgamma 2 , as well as with a number of other adapter proteins , some of which have not been previously described in platelets , including Cbl , Grb 2 , Gads , and SKAP HOM . ^^^ There was no detectable association between Grb 2 and SLP 76 in control or stimulated cells , suggesting that the interaction of LAT with Grb 2 is present in a separate complex to that of LAT Gads SLP 76 . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| Involvement of LAT , Gads , and Grb 2 in compartmentation of SLP 76 to the plasma membrane . ^^^ Moreover , we demonstrate that addition of both linker for activation of T cells ( LAT ) and Grb 2 related adaptor downstream of Shc ( Gads ) to SLP 76 allow SLP 76 to be recruited into GEMs , whereby the BCR function is reconstituted . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| Moreover , tyrosine phosphorylation of LAT led to the recruitment of intracytoplasmic signaling molecules including phospholipase Cgamma and Grb 2 . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| These dominant negative effects seemed to be dependent both on the ability of the membrane deletion mutant to reduce phosphorylation of endogenous LAT and to reduce interaction of endogenous LAT with PLC gamma 1 and Grb 2 . ^^^ Consistent with this , the redistribution of PLC gamma 1 and Grb 2 to glycolipid enriched microdomains , called lipid rafts , after stimulation was inhibited when the soluble form of LAT was overexpressed . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| Gads , a hematopoietic lineage specific Grb 2 family member , is involved in the signaling mediated by the TCR through its interactions with SLP 76 and LAT . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| Following TCR stimulation , LAT becomes tyrosine phosphorylated , creating docking sites for other signaling proteins such as phospholipase C gamma ( 1 ) ( PLC gamma ( 1 ) ) , Grb 2 , and Gads . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| In the Jurkat T cell line , the distal four tyrosines of LAT bind PLCgamma 1 , Grb 2 , and Gads . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| Receptor engagement leads to the phosphorylation of tyrosine residues present in the intracellular domain of LAT and formation of a multiprotein complex with other adapter molecules and enzymes including Grb 2 , Gads / SLP 76 and PLCgamma isoforms . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of LAT creates binding sites for the Src homology 2 ( SH 2 ) domains of other proteins , including PLC gamma 1 , Grb 2 , Gads , Grap , 3BP2 , and Shb , and indirectly binds SOS , c Cbl , Vav , SLP 76 , and Itk . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| CD 2 crosslinking markedly induced tyrosine phosphorylation of Cbl associated with Grb 2 or CrkL , Shc and LAT , compared with IL 2 stimulation . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| Gads , a member of the Grb 2 family of SH 2 and SH 3 domain containing adaptors , mediates the formation of a complex between LAT and SLP 76 that is essential for signal propagation from the TCR . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| Upon T cell antigen receptor ( TCR / CD3 ) engagement , LAT is phosphorylated by protein tyrosine kinases ( PTK ) and binds to the adaptors Gads and Grb 2 , as well as to phospholipase Cgamma 1 ( PLCgamma 1 ) , thereby facilitating the recruitment of key signal transduction components to drive T cell activation . ^^^ The LAT tyrosine residues Y ( 132 ) , Y ( 171 ) , Y ( 191 ) , and Y ( 226 ) have been shown previously to be critical for binding to Gads , Grb 2 , and PLCgamma 1 . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| Within seconds of contacting coverslips coated with stimulatory antibodies , T cells developed small , dynamically regulated clusters which were enriched in the TCR , phosphotyrosine , ZAP 70 , LAT , Grb 2 , Gads , and SLP 76 , excluded the lipid raft marker enhanced yellow fluorescent protein GPI , and were competent to induce calcium elevations . ^^^ LAT , Grb 2 , and Gads were transiently associated with the TCR . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| However , like LAT , LAX has tyrosine motifs for binding Grb 2 , Gads , and the p 85 subunit of phosphatidylinositol 3 kinase . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| Grb 2 , Gads , and PLC gamma 1 associated with LAT preferentially via different sets of tyrosine residues ; however , they failed to interact with LAT mutants containing only one tyrosine . ^^^ LAT mutants that were capable of binding Grb 2 and PLC gamma 1 could reconstitute T cell activation in LAT deficient cells and thymocyte development in LAT deficient mice . . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| We found that the phospholipase C gamma docking site of LAT and different Grb2 / Gads docking sites function in an interdependent fashion and synergize to accumulate LAT , Grb 2 , and phospholipase C gamma in TCR signaling assemblies . ^^^ Two dimensional gels showed that Grb 2 is a predominant cytoplasmic adaptor in the isolated LAT signaling complexes , whereas Gads , Crk 1 , and Grap are present in lower amounts . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of LAT at multiple tyrosines creates binding sites for the adaptors Gads and Grb 2 , leading to nucleation of multiprotein signaling complexes . ^^^ The structures reveal the basis for preferential recognition of specific LAT sites by Gads , as well as for the relatively greater promiscuity of the related adaptor Grb 2 , whose binding also requires asparagine at position +2 C terminal to the phosphorylated tyrosine . . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| The activated kinases then in turn phosphorylate and activate other enzymes [ phospholipase Cgamma ( PLCgamma ) isoforms , phosphatidylinositol 3 kinase ( PI3K ) isoforms , protein kinase C ( PKC ) isoforms , Bruton ' s tyrosine kinase ( Btk ) and others ] , adaptors [ linker for activation of T cells ( LAT ) , Cbl , Grb 2 and others ] and GTP exchange factors / GTPases ( Vav , Ras , Rho , and others ) , and subsequently induce the mobilization of stored and extracellular Ca ( 2+ ) . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| In T lymphocytes , Ras can be activated by two Ras exchange factors , SOS and RasGRP 1 , which are recruited through the adapters Grb 2 and LAT and via the second messenger diacylglycerol ( DAG ) , respectively . ^^^ |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|