| Interacting proteins: O14939 and P62993 |
Pubmed |
SVM Score :0.60801417 |
| Systematic co immunoprecipitation immunoblotting experiments pointed at a physical association between PLD 2 , Grb 2 , and PTP1B in both physiological conditions and in overexpressed cells . 0.60801417^^^ |
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| Interacting proteins: O14939 and P62993 |
Pubmed |
SVM Score :0.0 |
| Our laboratory has recently reported that the enzyme phospholipase D 2 ( PLD 2 ) exists as a ternary complex with PTP1b and the growth factor receptor bound protein 2 ( Grb 2 ) . ^^^ We have identified residues Y ( 169 ) and Y ( 179 ) in the PLD 2 protein as being essential for the Grb 2 interaction . ^^^ We present evidence indicating that Y ( 169 ) and Y ( 179 ) are located within two consensus sites in PLD 2 that mediate an SH 2 interaction with Grb 2 . ^^^ Phenylalanine replacement of Y ( 169 ) ( PLD 2 Y169F ) or Y ( 179 ) ( PLD 2 Y179F ) reduced Grb 2 binding while simultaneous mutation completely abolished it . ^^^ Interestingly , binding of Grb 2 to PLD 2 occurs irrespectively of lipase activity , since Grb 2 binds to catalytically inactive PLD 2 mutants . ^^^ |
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