Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: Q12967 and P62834	Pubmed	SVM Score :0.0
We have recently found that ralGDS family members ( RGL and ralGDS ) are putative effector proteins of ras p 21 . rap 1 p21 is a small GTP binding protein which has the same amino acid sequence as the effector loop of ras p 21 . ^^^
Interacting proteins: Q12967 and P62834	Pubmed	SVM Score :0.0
This new GDS , ralGDS , is at least 20 fold more active on the ralA and ralB GTPases than on any other GTPase tested , including other members of the ras family ( H ras , N ras , K ras , R ras , rap1a and rap 2 ) , members of the rho family ( rhoA , rhoB and CDC 42 Hs ) and members of the rab family ( rab3a and ypt 1 ) . ^^^
Interacting proteins: Q12967 and P62834	Pubmed	SVM Score :0.0
RalGDS like factor ( Rlf ) is a novel Ras and Rap 1A associating protein . ^^^
Interacting proteins: Q12967 and P62834	Pubmed	SVM Score :0.0
Identification of a novel RalGDS related protein as a candidate effector for Ras and Rap 1 . ^^^
Interacting proteins: Q12967 and P62834	Pubmed	SVM Score :0.0
We have developed a novel assay to monitor the active , GTP bound form of Rap 1 based on the differential affinity of Rap1GTP and Rap1GDP for the Rap binding domain of RalGDS ( RBD ) . ^^^
Interacting proteins: Q12967 and P62834	Pubmed	SVM Score :0.0
The Ras and Rap1A proteins can bind to the Raf and RalGDS families . ^^^ RasG12V ( E31K ) , RasG12V ( E31A ) , and Rap1A stimulate the RalGDS action more efficiently than the wild type Ras in the liposome reconstitution assay . ^^^ All of these results clearly show that the sharp contrast between the characteristics of Ras and Rap1A , with respect to the interactions with Raf 1 and RalGDS , depends on their residues at position 31 . . ^^^
Interacting proteins: Q12967 and P62834	Pubmed	SVM Score :0.0
Using the Rap 1 binding domain of RalGDS ( RalGDS RBD ) as an activation specific probe for Rap 1 , we have investigated the regulation of Rap 1 activity in primary human neutrophils . ^^^
Interacting proteins: Q12967 and P62834	Pubmed	SVM Score :0.0
In COS cells , Ral GDP dissociation stimulator ( RalGDS ) induced Ral activation was stimulated by RasG12V or a Rap1 / Ras chimera in which the N terminal region of Rap 1 was ligated to the C terminal region of Ras but not by Rap1G12V or a Ras / Rap1 chimera in which the N terminal region of Ras was ligated to the C terminal region of Rap 1 , although RalGDS interacted with these small GTP binding proteins . ^^^ These results indicate that Ras recruits RalGDS to the plasma membrane and that the translocated RalGDS induces the activation of Ral , but that Rap 1 does not activate Ral due to distinct subcellular localization . . ^^^
Interacting proteins: Q12967 and P62834	Pubmed	SVM Score :0.0
Glu 31 of Ras discriminates Ras from a Ras homologue , Rap 1 , with Lys 31 , with respect to RalGDS and RGL binding ; the E31K mutation of Ras potentiates the abilities to bind RGL RBD and RalGDS RBD . ^^^
Interacting proteins: Q12967 and P62834	Pubmed	SVM Score :0.0
Using the selective interactions of Ras and Krev 1 ( Rap1A ) with Raf , RalGDS , and Krit 1 as a model , we systematically compared LexA and cI fused baits and reporters . ^^^
Interacting proteins: Q12967 and P62834	Pubmed	SVM Score :0.0
In spite of the close homology between p 21 ( ras ) and rap 1 , the S17N mutant of rap 1 was not dominant negative because ( 1 ) overexpression of rap 1 ( S17N ) failed to inhibit A ( 2A ) receptor dependent MAP kinase activation , ( 2 ) rap 1 ( S17N ) was recovered in the active form with a GST fusion protein comprising the rap 1 binding domain of ralGDS after A ( 2A ) receptor activation , and ( 3 ) A ( 2A ) agonists promoted the association of rap 1 ( S17N ) with the 68 kDa isoform of B raf in CHO cells . ^^^
Interacting proteins: Q12967 and P62834	Pubmed	SVM Score :0.0
High pressure NMR study of the complex of a GTPase Rap1A with its effector RalGDS . ^^^
Interacting proteins: Q12967 and P62834	Pubmed	SVM Score :0.0
Furthermore , it is shown that Rap 1 , which was originally identified as a Ras antagonist , can block Ras mediated RalGDS signaling only when RalGDS contains an intact RBD . ^^^ Conversely , the Ras 10 RalGDS complex has a short lifetime of 0 . 1 s and Rap 1 forms a long lived complex with RalGDS , possibly explaining its antagonistic effect on Ras . . ^^^
Interacting proteins: Q12967 and P62834	Pubmed	SVM Score :0.0
Inhibition of Rap 1 either by Rap GTPase activating protein ( RapGAP ) or the Rap 1 binding domain of RalGDS abolished both Mn ( 2+ ) and KIM 185 ( anti LFA 1 ) induced LFA 1 mediated cell adhesion to intercellular adhesion molecule 1 . ^^^
Interacting proteins: Q12967 and P62834	Pubmed	SVM Score :0.0
Dictyostelium Rap 1 formed a complex with the Ras binding domain of RalGDS only when it was in a GTP bound state . ^^^
Interacting proteins: Q12967 and P62834	Pubmed	SVM Score :0.0
The interactions of H Ras , R Ras , and Rap1A with the Ras binding domains ( RBD ) of the c Raf kinase and of the Ral guanine nucleotide exchange factor ( RGF ) was studied biochemically in solution . ^^^ Interestingly , whereas H Ras binds with high affinity ( KD = 20 nM ) to Raf RBD and with low affinity ( KD = 1 microM ) to RGF RBD , Rap1A shows the opposite behavior . ^^^ The interaction between Rap1A and RGF RBD shows similar characteristics to the Ras Raf interaction because it is blocked by mutations in the effector region ( D38A ) and it inhibits the dissociation of guanine nucleotide , which is the basis for the quantitative measurements in this work . ^^^ Furthermore , the binding of RGF RBD inhibits the interaction between Rap1A and Rap GAP . ^^^
Interacting proteins: Q12967 and P62834	Pubmed	SVM Score :0.0
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Interacting proteins: Q12967 and P62834	Pubmed	SVM Score :0.0
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Interacting proteins: Q12967 and P62834	Pubmed	SVM Score :0.0
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Interacting proteins: Q12967 and P62834	Pubmed	SVM Score :0.0
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Interacting proteins: Q12967 and P62834	Pubmed	SVM Score :0.0
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Interacting proteins: Q12967 and P62834	Pubmed	SVM Score :0.0
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Interacting proteins: Q12967 and P62834	Pubmed	SVM Score :0.0
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Interacting proteins: Q12967 and P62834	Pubmed	SVM Score :0.0
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Interacting proteins: Q12967 and P62834	Pubmed	SVM Score :0.0
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Interacting proteins: Q12967 and P62834	Pubmed	SVM Score :0.0
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