| Interacting proteins: P62834 and P55196 |
Pubmed |
SVM Score :0.53995132 |
| We further demonstrate that both Ras and Rap 1 interact with full length AF 6 in vivo in mammalian cells and that a fraction of Rap 1 colocalizes with AF 6 at the membrane . 0.53995132^^^ |
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| Interacting proteins: P62834 and P55196 |
Pubmed |
SVM Score :0.7293891 |
| Studies on the mode of action of the nectin afadin system using cell free assay revealed that afadin associated with nectin bound Rap 1 activated by trans interacting nectin , interacted with p120ctn , and strengthened the binding of p120ctn to E cadherin , eventually reducing non trans interacting E cadherin endocytosis . 0.7293891^^^ |
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| Interacting proteins: P62834 and P55196 |
Pubmed |
SVM Score :0.0 |
| AF 6 controls integrin mediated cell adhesion by regulating Rap 1 activation through the specific recruitment of Rap1GTP and SPA 1 . ^^^ In the present study , we showed that SPA 1 , a Rap 1 GTPase activating protein ( GAP ) , was bound to a cytoskeleton anchoring protein AF 6 . ^^^ The motif was conserved among Rap 1 GAPs , and it was shown that rapGAP 1 was bound to AF 6 comparably with SPA 1 . ^^^ These results suggested that AF 6 could control integrin mediated cell adhesion by regulating Rap 1 activation through the recruitment of both SPA 1 and Rap1GTP via distinct domains . . ^^^ |
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| Interacting proteins: P62834 and P55196 |
Pubmed |
SVM Score :0.0 |
| The AF 6 homolog canoe acts as a Rap 1 effector during dorsal closure of the Drosophila embryo . ^^^ Here , we demonstrate that Canoe ( Cno ) , the Drosophila homolog of mammalian junctional protein AF 6 , acts as an effector of Rap 1 in vivo . ^^^ |
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| Interacting proteins: P62834 and P55196 |
Pubmed |
SVM Score :0.0 |
| AF 6 negatively regulates Rap 1 induced cell adhesion . ^^^ Here we show that knockdown of AF 6 in T cells by RNA interference enhanced Rap 1 induced integrin mediated cell adhesion , whereas overexpression of AF 6 had the opposite effect . ^^^ Like AF 6 , protein KIAA 1849 contains a Ras association domain and interacted with Rap 1 . ^^^ We proposed that AF 6 inhibits Rap mediated cell adhesion by sequestering RapGTP in an unproductive complex and thus prevents the interaction of Rap 1 not only with effectors that mediate adhesion but also with Rap GTPase activating proteins . ^^^ |
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| Interacting proteins: P62834 and P55196 |
Pubmed |
SVM Score :0.0 |
| This assembly was mediated by the IQGAP 1 dependent actin cytoskeleton , which was organized by Cdc 42 and Rac small G proteins that were activated by the action of trans interacting nectin through c Src and Rap 1 small G protein in an afadin independent manner . ^^^ However , Rap 1 bound to afadin , and this Rap 1 afadin complex then interacted with p 120 ( ctn ) associated with non trans interacting E cadherin , thereby causing the trans interaction of E cadherin . ^^^ |
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| Interacting proteins: P62834 and P55196 |
Pubmed |
SVM Score :0.0 |
| Notably , among the Ras related proteins AF 6 binds most tightly to Rap1A which could imply a role of Rap1A in AF 6 regulation . . ^^^ |
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| Interacting proteins: P62834 and P55196 |
Pubmed |
SVM Score :0.0 |
| Activity dependent dendritic spine structural plasticity is regulated by small GTPase Rap 1 and its target AF 6 . ^^^ Rap 1 bimodally regulates spine morphology : activated Rap 1 recruits the PDZ domain containing protein AF 6 to the plasma membrane and induces spine neck elongation , while inactive Rap 1 dissociates AF 6 from the membrane and induces spine enlargement . ^^^ |
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| Interacting proteins: P62834 and P55196 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P62834 and P55196 |
Pubmed |
SVM Score :0.0 |
| NA |
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