| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.94902761 |
| Although previous data suggest that Rpb 7 can stably interact with Pol 2 only as a heterodimer with Rpb 4 , RPB 7 is essential for viability , whereas RPB 4 is essential only during some stress conditions . 0.94902761^^^ |
|
| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.72563042 |
| Deletions in the amino and carboxyl terminal domains of Rpb 7 abolished its interaction with Rpb 4 . 0.72563042^^^ Complete abolishment of interaction between Rpb 4 and Rpb 7 occurred by truncation of 1 106 , 1 142 , 108 221 , 172 221 or 198 221 amino acids of Rpb 4 . 0.57706187^^^ |
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| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.63016798 |
| However , hsRPB 4 associates strongly and specifically with hsRPB 7 when expressed in yeast or in mammalian cells and copurifies with intact pol 2 . hsRPB 4 expression in humans parallels that of hsRPB 7 , supporting the idea that the two proteins may possess associated functions . 0.63016798^^^ |
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| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.0 |
| However , at temperature extremes and during maintenance at stationary phase , hsRPB 7 containing yeast cells lose viability rapidly , stress sensitive phenotypes reminiscent of those associated with deletion of the RPB 4 subunit with which RPB 7 normally complexes . ^^^ Two hybrid analysis revealed that although hsRPB 7 and RPB 4 interact , the association is of lower affinity than the RPB 4 RPB7 interaction , providing a probable mechanism for the failure of hsRPB 7 to fully function in yeast cells at high and low temperatures . ^^^ |
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| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.0 |
| RPB 7 also appears to interact with RNA polymerase 2 in a manner dependent upon RPB 4 , since RNA polymerase 2 purified from cells lacking RPB 4 also lacks RPB 7 . ^^^ Loss of both the RPB 4 and the RPB 7 genes causes lethality . ^^^ These results suggest that RPB 7 contributes to the function of RNA polymerase 2 in the absence of RPB 4 either in a manner independent of its association with the enzyme or by directly binding to the enzyme in a manner independent of its association with RPB4 . . ^^^ |
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| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.0 |
| Interaction with two polymerase subunits , Rpb 4 and Rpb 7 , favors the closed conformation , and binding of general transcription factor TFIIE may do so as well . ^^^ The effect of Rpb 4 and Rpb 7 , together with previous biochemical evidence , leads to the conclusion that the open to closed transition is a crucial step in the transcription initiation process . . ^^^ |
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| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.0 |
| Two small subunits in Arabidopsis RNA polymerase 2 are related to yeast RPB 4 and RPB 7 and interact with one another . ^^^ Unlike yeast RPB 4 and RPB 7 , which dissociate from RNA polymerase 2 under mildly denaturing conditions , plant subunits related to RPB 4 and RPB 7 are more stably associated with the enzyme . ^^^ Recombinant AtRPB15 . 9 formed stable complexes with AtRPB19 . 5 ( i . e . a subunit related to yeast RPB 7 ) in vitro as did recombinant yeast RPB 4 and RPB 7 subunits . ^^^ Stable heterodimers were also formed between AtRPB 15 . 9 and yeast RPB 7 and between yeast RPB 4 and AtRPB19 . 5 . . ^^^ |
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| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.0 |
| Structure of wild type yeast RNA polymerase 2 and location of Rpb 4 and Rpb 7 . ^^^ A difference map between this structure and that of the polymerase lacking subunits Rpb 4 and Rpb 7 showed these two subunits forming part of the floor of the DNA binding ( active center ) cleft , and revealed a slight inward movement of the protein domain surrounding the cleft . ^^^ Surface plasmon resonance measurements showed that Rpb 4 and Rpb 7 stabilize a minimal pre initiation complex containing promoter DNA , TATA box binding protein ( TBP ) , transcription factor TFIIB and the polymerase . ^^^ These findings suggest that Rpb 4 and Rpb 7 play a role in coupling the entry of DNA into the active center cleft to closure of the cleft . ^^^ |
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| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.0 |
| Whereas RPB 7 is essential , RPB 4 is dispensable for cellular viability . ^^^ The heat growth defect of the rpb 4 null mutant can be partially suppressed by overexpression of RPB 7 , suggesting that Rpb4p maintains or stabilizes Rpb7p in the RNA polymerase . ^^^ |
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| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.0 |
| In contrast to the RPB 4 homologues from higher eukaryotes , however , S . pombe Rpb 4 formed stable hybrid heterodimers with S . cerevisiae RPB 7 , suggesting that S . pombe Rpb 4 is similar , in its structure and essential role in cell viability , to the corresponding subunits from higher eukaryotes . ^^^ |
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| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.0 |
| Suppression by RPB 7 was anticipated since its interaction with RPB 4 is well established both in vitro and in vivo . ^^^ |
|
| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.0 |
| Under certain circumstances , RPB 3 , RPB 4 , and RPB 7 were cross linked . ^^^ |
|
| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.0 |
| The Rpb 4 and Rpb 7 subunits of yeast RNA polymerase 2 form a heterodimeric complex essential for promoter directed transcription initiation in a reconstituted system . ^^^ Purified Rpb 4 Rpb7 complex exhibited both single strand DNA and RNA binding activities , and a small deletion in the putative OB fold nucleic acid binding surface of Rpb 7 abolished binding activity without affecting the stability of the Rpb 4 Rpb7 complex or its ability to associate with polymerase . ^^^ |
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| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.0 |
| The transcription of one group of genes including rpb 3 , rpb 4 , rpb 5 , rpb 6 , rpb 7 and rpb 10 is mainly initiated at a single site , while that of the other group of genes for rpb 1 , rpb 2 , rpb 8 , rpb 9 , rpb 11 and rpb 12 is initiated at multiple sites . ^^^ |
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| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.0 |
| Previous studies demonstrating a physical interaction between the EAD and the human RNA Polymerase 2 subunit hsRPB 7 suggest a crucial role for RPB 7 and its partner , RPB 4 , in EAD function . ^^^ |
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| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.0 |
| Surprisingly , the overexpression of RPB 7 ( the interacting partner of Rpb 4 ) does not rescue the activation defect of all the promoters tested , although it rescues the activation defect of the heat shock element containing promoter and the temperature sensitivity associated with RPB 4 deletion . ^^^ Overall , our results indicate that Rpb 4 and Rpb 7 play independent roles in transcriptional regulation of genes . . ^^^ |
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| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.0 |
| The eukaryotic subunits RPB 4 and RPB 7 form a heterodimer that reversibly associates with the RNA polymerase 2 core and constitute the only two components of the enzyme for which no structural information is available . ^^^ We have determined the crystal structure of the complex between the Methanococcus jannaschii subunits E and F , the archaeal homologs of RPB 7 and RPB 4 . ^^^ |
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| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.0 |
| We found that two RNA polymerase 3 subunits , referred to as RPC 8 and RPC 9 , displayed sequence similarity to the RNA polymerase 2 RPB 7 and RPB 4 subunits , respectively . ^^^ RPC 8 and RPC 9 associated with each other , paralleling the association of the RNA polymerase 2 subunits , and were thus paralogues of RPB 7 and RPB 4 . ^^^ |
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| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.0 |
| Because the N terminal half of A 43 is clearly related to the pol 2 Rpb 7 subunit , we propose that the A 43 A14 pair is likely the pol 1 counterpart of the Rpb 7 Rpb4 heterodimer , although A 14 distinguishes from Rpb 4 by specific sequence and structure features . ^^^ |
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| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.0 |
| Purification of mutant RNA polymerase 2 revealed that two subunits , Rpb 4 and Rpb 7 , are selectively lost in mutant cells . ^^^ Rpb 4 and Rpb 7 are present at substoichiometric levels , form a dissociable subcomplex , are required for RNA polymerase 2 activity at high temperatures , and have been implicated in the regulation of enzyme activity . ^^^ |
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| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.0 |
| Recently it has emerged that this heterodimer also has a counterpart in the other eukaryotic RNA polymerases : in particular two subunits of RNA polymerase 1 ( A 14 and A 43 ) display genetic and biochemical characteristics that are similar to those of the RPB 4 and RPB 7 subunits , despite the fact that only A 43 shows some sequence homology to RPB 7 . ^^^ |
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| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.0 |
| Our deletion analysis and molecular modeling results show that the N and C terminal conserved regions of Rpb 4 are involved in interaction with Rpb 7 , the Rpb 4 interacting partner in the RNA polymerase 2 . ^^^ |
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| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.0 |
| The subcomplex of Rpb 4 and Rpb 7 subunits of RNA pol 2 in Saccharomyces cerevisiae is known to be an important determinant of transcription under a variety of physiological stresses . ^^^ In S . cerevisiae , RPB 7 is essential for cell viability while rpb 4 null strains are temperature sensitive at low and high temperatures . ^^^ We also show that high expression levels of S . cerevisiae RPB 7 and its homologs rescue the sporulation defect of rpb 4 homozygous null diploids , but only some of them cause significant enhancement of the pseudohyphal phenotype . ^^^ |
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| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.0 |
| Rpb 4 and Rpb 7 : subunits of RNA polymerase 2 and beyond . ^^^ Rpb 4 and Rpb 7 form a dissociable heterodimer ( Rpb4 / 7 ) . ^^^ |
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| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.0 |
| Rpb 4 and Rpb 7 : a sub complex integral to multi subunit RNA polymerases performs a multitude of functions . ^^^ Rpb 4 and Rpb 7 , are conserved subunits of RNA polymerase 2 that play important roles in stress responses such as growth at extreme temperatures , recovery from stationary phase , sporulation and pseudohyphal growth . ^^^ |
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| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.0 |
| The Rpb 4 and Rpb 7 subunits of eukaryotic RNA polymerase 2 ( RNAP ( 2 ) ) form a heterodimer that protrudes from the 10 subunit core of the enzyme . ^^^ |
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| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.0 |
| Structural studies of RNA polymerase 2 have suggested two possible exit paths for the nascent RNA : groove 1 , which points toward the subcomplex of subunits Rpb 4 and Rpb 7 , and groove 2 , which points toward Rpb 8 . ^^^ |
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| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.0 |
| Nine subunits homologous to the other eukaryotic RNA Pol 2 , namely RPB 1 , RPB 2 , RPB 3 , RPB 4 , RPB 5 , RPB 6 , RPB 7 , RPB 8 and RPB 11 , were identified in the purified complex . ^^^ |
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| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O15514 and P62487 |
Pubmed |
SVM Score :0.0 |
| NA |
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