| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| Although exogenous recombinant ARNO promotes only a small increase in ARF 6 activation in the presence of activated LH / CG R , hCG stimulated ARF 6 activation is reduced to basal levels by catalytically inactive ARF nucleotide binding site opener . ^^^ |
|
| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| Overexpression of the inactive form of the GTP exchange factor ARNO ( ARF nucleotide binding site opener ) or inactive ARF 6 enhanced dendritic branching , whereas coexpression of either Rac 1 ( a member of the Rho family of small GTPases known to control dendritic dynamics and growth ) or active ARF 6 with inactive ARNO eliminated the enhanced branching effect . ^^^ |
|
| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| Stimulation with a secretagogue triggers the recruitment of secretory granules to the cell periphery and the concomitant activation of ARF 6 by the plasma membrane associated guanine nucleotide exchange factor , ARF nucleotide binding site opener ( ARNO ) . ^^^ |
|
| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| The functional relationship between IPCEF 1 and cytohesin 2 was assessed by studying the effect of IPCEF 1 on the in vitro and in vivo stimulation of ADP ribosylation factor 6 GTP formation by cytohesin 2 . . ^^^ |
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| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| In neuroendocrine PC 12 cells , SCAMP 2 colocalizes on the cell surface with three other proteins required for dense core vesicle exocytosis : phospholipase D 1 ( PLD 1 ) , the small GTPase Arf 6 , and Arf 6 guanine nucleotide exchange protein ARNO . ^^^ |
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| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| ARNO is a guanine nucleotide exchange factor for ADP ribosylation factor 6 . ^^^ We report here that in vitro ARNO can stimulate nucleotide exchange on both ARF 1 and ARF 6 . ^^^ It is therefore likely that ARNO functions in plasma membrane events by modulating the activity of ARF 6 in vivo . ^^^ |
|
| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| Our data strongly suggest that ARNO binds PIP 3 in vivo and that this interaction causes a translocation of ARNO to the plasma membrane where it might activate ARF 6 and regulate subsequent plasma membrane cycling events . . ^^^ |
|
| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| We have previously shown that ARNO localizes to the plasma membrane in vivo and efficiently catalyzes ARF 6 nucleotide exchange in vitro . ^^^ To determine whether ARNO is an upstream regulator of ARF 6 in vivo , we examined the distribution of actin in HeLa cells overexpressing ARNO . ^^^ However , treatment of ARNO transfectants with the PKC agonist phorbol 12 myristate 13 acetate results in the dramatic redistribution of ARNO , ARF 6 , and actin into membrane protrusions resembling lamellipodia . ^^^ Taken together , these data suggest that ARNO represents an important link between cell surface receptors , ARF 6 , and the actin cytoskeleton . . ^^^ |
|
| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| EFA 6 promotes efficient guanine nucleotide exchange on ARF 6 and is distinct from the ARNO family of ARF 1 exchange factors . ^^^ |
|
| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| ARNO is a member of a family of guanine nucleotide exchange factors that activate small GTPases called ADP ribosylation factors ( ARFs ) [ 1 ] [ 2 ] [ 3 ] , which regulate vesicular trafficking and , in one case ( ARF 6 ) , also regulate cortical actin structure [ 4 ] . ^^^ ARNO is located at the plasma membrane , and in the presence of activated protein kinase C ( PKC ) can induce cortical actin rearrangements reminiscent of those produced by active ARF 6 [ 5 ] [ 6 ] [ 7 ] [ 8 ] . ^^^ |
|
| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| ARNO and GRP 1 are nucleotide exchange factors for the small GTP binding protein ADP ribosylation factor 6 ( ARF 6 ) . ^^^ |
|
| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| The possibility that the plasma membrane associated ARNO participates in the exocytotic pathway by activating ARF 6 and downstream PLD is discussed . . ^^^ |
|
| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| In this review we have described data supporting the conclusion that PtdIns ( 3 , 4 , 5 ) P 3 may regulate the activation state of ARF 6 through an ability to recruit the ARF exchange factors ARNO , GRP 1 and cytohesin 1 to the plasma membrane . ^^^ |
|
| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| Catalytically inactive constructs of PLD 2 and RhoA , but not PLD 1 , ADP ribosylation factor 1 ( ARF 1 ) , ARF 6 , or ADP ribosylation factor nucleotide exchange factor ( ARNO ) blocked Ang 2 induced PLD activity in SHR and WKY PGSMCs . ^^^ |
|
| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| Intra endosomal pH sensitive recruitment of the Arf nucleotide exchange factor ARNO and Arf 6 from cytoplasm to proximal tubule endosomes . ^^^ We show here that an Arf nucleotide exchange factor , ARNO ( ADP ribosylation factor nucleotide site opener ) as well as Arf 6 and Arf 1 small GTPases are located in the kidney proximal tubule receptor mediated endocytosis pathway , and that ARNO and Arf 6 recruitment from cytosol to endosomes is pH dependent . ^^^ In proximal tubules in situ , ARNO and Arf 6 partially co localized with the 5 ATPase in apical endosomes in proximal tubules . ^^^ By Western blot analysis ARNO , Arf 6 , and Arf 1 were detected both in purified endosomes and in proximal tubule cytosol . ^^^ A translocation assay showed that ATP driven endosomal acidification triggered the recruitment of ARNO and Arf 6 from proximal tubule cytosol to endosomal membranes . ^^^ |
|
| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| Activation of ARF 6 by ARNO stimulates epithelial cell migration through downstream activation of both Rac 1 and phospholipase D . ^^^ This transition requires ARNO catalytic activity , which we show leads to enhanced activation of endogenous ARF 6 , but not ARF 1 , using a novel pulldown assay . ^^^ Finally , ARNO induced activation of ARF 6 also results in increased activation of phospholipase D ( PLD ) , and inhibition of PLD activity also inhibits motility . ^^^ |
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| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| The importance of regulation of ARF 6 function is shown by the ability of the ARF GTPase activating protein GIT 1 to inhibit and of the ARF nucleotide exchange factor , ARNO , to enhance receptor endocytosis . ^^^ Upon agonist stimulation of the receptor , beta arrestin also interacts with the GDP liganded form of ARF 6 , thereby facilitating ARNO promoted GTP loading and activation of the G protein . ^^^ Thus , the agonist driven formation of a complex including beta arrestin , ARNO , and ARF 6 provides a molecular mechanism that explains how the agonist stimulated receptor recruits a small G protein necessary for the endocytic process and controls its activation . . ^^^ |
|
| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| Apically added S . typhimurium promoted the translocation of ARF 6 and its exchange factor ARNO to the apical surface . ^^^ |
|
| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| Recent studies have shown that ARNO and ARF 6 play significant roles in coordinating this transition , providing new insight into the interplay between the Rho and ARF families of GTPases . . ^^^ |
|
| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| ARNO , a potential guanine nucleotide exchange factor for ARF 6 , is expressed and localized in the plasma membrane of chromaffin cells . ^^^ We propose that ARNO ARF 6 participate in the exocytotic reaction by controlling the plasma membrane bound PLD 1 . ^^^ |
|
| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| ARNO and ARF 6 regulate axonal elongation and branching through downstream activation of phosphatidylinositol 4 phosphate 5 kinase alpha . ^^^ To investigate the role of these molecules in axonogenesis , we expressed ARNO and ARF 6 in cultured rat hippocampal neurons . ^^^ Expression of catalytically inactive ARNO or dominant negative ARF 6 resulted in enhanced axonal extension and branching and this effect was abrogated by coexpression of constitutively active ARF 6 . ^^^ We sought to identify the downstream effectors of ARF 6 during neurite extension by coexpressing phosphatidyl inositol 4 phosphate 5 Kinase alpha [ PI ( 4 ) P 5 Kinase alpha ] with catalytically inactive ARNO and dominant negative ARF 6 . ^^^ These results suggest that ARNO and ARF 6 , through PI ( 4 ) P 5 Kinase alpha , regulate axonal elongation and branching during neuronal development . . ^^^ |
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| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| Moreover , the fact that HERC 1 overexpression does not stimulate protrusion formation in the absence of AlF ( 4 ) ( ) , in conditions where ARNO does , indicates that HERC 1 is not acting as an ARF 6 GEF in this system , but that instead its recruitment takes place downstream of ARF 6 activation . ^^^ |
|
| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| We show that HERC 1 , through its RLD 1 , stimulates GDP release from ARF 6 but , unexpectedly , it inhibits GDP / GTP exchange on ARF 6 under conditions where ARNO stimulates it . ^^^ |
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| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| Thus mobilization of K ( Ca ) was blocked by treatments than inhibit ADP ribosylation factor 6 ( ARF 6 ) signaling , including overexpression of dominant negative ARF 6 , dominant negative ARNO , or dominant negative phospholipase D 1 . ^^^ |
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| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| The DOCK180 / Elmo complex couples ARNO mediated Arf 6 activation to the downstream activation of Rac 1 . ^^^ Together , these data suggest that ARNO and ARF 6 coordinate with the Dock180 / Elmo complex to promote Rac activation at the leading edge of migrating cells . . ^^^ |
|
| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| Functional assay of ARNO and ARF 6 in neurite elongation and branching . ^^^ |
|
| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| ARF 6 is activated by the nucleotide exchange factor ARNO following docking of granules to the plasma membrane . ^^^ |
|
| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| V ATPase interacts with ARNO and Arf 6 in early endosomes and regulates the protein degradative pathway . ^^^ The recruitment of the small GTPase Arf 6 and ARNO from cytosol to endosomal membranes is driven by 5 ATPase dependent intra endosomal acidification . ^^^ Here , we demonstrate that Arf 6 interacts with the c subunit , and ARNO with the a 2 isoform of 5 ATPase . ^^^ They also indicate that 5 ATPase could modulate membrane trafficking by recruiting and interacting with ARNO and Arf 6 ; characteristics that are consistent with the role of 5 ATPase as an essential component of the endosomal pH sensing machinery . . ^^^ |
|
| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| Expression of ARNO stimulates apical endocytosis of the polymeric immunoglobulin receptor , and coexpression of ARF 6 with ARNO leads to a synergistic stimulation of apical endocytosis . ^^^ We conclude that ARNO acts together with ARF 6 to regulate apical endocytosis . . ^^^ |
|
| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99418 and P62330 |
Pubmed |
SVM Score :0.0 |
| NA |
|