| Interacting proteins: Q13393 and P62330 |
Pubmed |
SVM Score :0.0 |
| Colocalization of phospholipase D 1 and GTP binding defective mutant of ADP ribosylation factor 6 to endosomes and lysosomes . ^^^ |
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| Interacting proteins: Q13393 and P62330 |
Pubmed |
SVM Score :0.0 |
| Crosstalk between ARF 6 and protein kinase Calpha in Fc ( gamma ) RI mediated activation of phospholipase D 1 . ^^^ Here , we show that immune complex aggregation of Fc ( gamma ) RI stimulates the association of phospholipase D 1 with ARF 6 and protein kinase Calpha . ^^^ Moreover , treatment of cells with butan 1 ol or phospholipase D 1 antisense oligonucleotides inhibits translocation of PKCdelta , epsilon , and zeta but had no effect on the association of PKCalpha or ARF 6 with phospholipase D 1 . ^^^ These data indicate that association with ARF 6 and PKCalpha plays a role in coupling Fc ( gamma ) RI to phospholipase D 1 activation and that PLD 1 lies upstream of all Fc ( gamma ) RI mediated PKC activity . . ^^^ |
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| Interacting proteins: Q13393 and P62330 |
Pubmed |
SVM Score :0.0 |
| Thus mobilization of K ( Ca ) was blocked by treatments than inhibit ADP ribosylation factor 6 ( ARF 6 ) signaling , including overexpression of dominant negative ARF 6 , dominant negative ARNO , or dominant negative phospholipase D 1 . ^^^ TGF ( beta ) 1 , the effects of which on K ( Ca ) are much slower in onset , is unable to selectively mobilize channels in the post Golgi pool , and its effects on K ( Ca ) are completely blocked by inhibition of microtubules , Golgi function and also by plasma membrane ARF 6 and phospholipase D 1 signaling . . ^^^ |
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| Interacting proteins: Q13393 and P62330 |
Pubmed |
SVM Score :0.0 |
| SCAMP 2 interacts with Arf 6 and phospholipase D 1 and links their function to exocytotic fusion pore formation in PC 12 cells . ^^^ In neuroendocrine PC 12 cells , SCAMP 2 colocalizes on the cell surface with three other proteins required for dense core vesicle exocytosis : phospholipase D 1 ( PLD 1 ) , the small GTPase Arf 6 , and Arf 6 guanine nucleotide exchange protein ARNO . ^^^ |
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| Interacting proteins: Q13393 and P62330 |
Pubmed |
SVM Score :0.0 |
| Using chromaffin and PC 12 cells , we have recently described that the granule associated GTPase ARF 6 plays a crucial role in exocytosis by activating phospholipase D 1 at the plasma membrane and , presumably , promoting the fusion reaction between the two membrane bilayers . ^^^ |
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| Interacting proteins: Q13393 and P62330 |
Pubmed |
SVM Score :0.0 |
| Catalytically inactive constructs of PLD 2 and RhoA , but not PLD 1 , ADP ribosylation factor 1 ( ARF 1 ) , ARF 6 , or ADP ribosylation factor nucleotide exchange factor ( ARNO ) blocked Ang 2 induced PLD activity in SHR and WKY PGSMCs . ^^^ |
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| Interacting proteins: Q13393 and P62330 |
Pubmed |
SVM Score :0.0 |
| Using a mutant of ARF 6 specifically impaired for PLD 1 stimulation , we find that ARF 6 is functionally linked to phospholipase D ( PLD ) 1 in the exocytotic machinery . ^^^ Finally , we show that ARNO , ARF 6 , and PLD 1 colocalize at sites of exocytosis , and we demonstrate direct interaction between ARF 6 and PLD 1 in stimulated cells . ^^^ Together , these results provide the first direct evidence that ARF 6 plays a role in calcium regulated exocytosis in neuroendocrine cells , and suggest that ARF 6 stimulated PLD 1 activation at the plasma membrane and consequent changes in membrane phospholipid composition are critical for formation of the exocytotic fusion pore . . ^^^ |
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| Interacting proteins: Q13393 and P62330 |
Pubmed |
SVM Score :0.0 |
| PLD 1 and PLD 2 are both expressed in mast cells and green fluorescent protein tagged proteins were used to identify PLD 2 localizing to membrane ruffles of antigen stimulated mast cells together with endogenous ADP ribosylation factor 6 ( ARF 6 ) . ^^^ |
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| Interacting proteins: Q13393 and P62330 |
Pubmed |
SVM Score :0.0 |
| In agonist stimulated cells , however , there is specificity , with , for example in RBL 2H3 cells , antigen stimulating the activation of PLD 1 by association with Arf 6 , Rac 1 and protein kinase Calpha . ^^^ |
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| Interacting proteins: Q13393 and P62330 |
Pubmed |
SVM Score :0.0 |
| We propose that ARNO ARF 6 participate in the exocytotic reaction by controlling the plasma membrane bound PLD 1 . ^^^ |
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| Interacting proteins: Q13393 and P62330 |
Pubmed |
SVM Score :0.0 |
| Constitutive class 1 mGluR internalization is not dependent on the downstream Ral effector proteins Ral binding protein 1 and PLD 1 or either ADP ribosylation factors ARF 1 or ARF 6 . ^^^ |
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| Interacting proteins: Q13393 and P62330 |
Pubmed |
SVM Score :0.0 |
| When wild type or dominant negative ARF 6 and PLD 1 or PLD 2 were co expressed , they had a similar localization in cytosolic particles and at the cell periphery . ^^^ In contrast , dominant active ARF 6 caused cell shrinkage and had a similar localization with PLD 1 and PLD 2 in dense structures , containing the trans Golgi apparatus and actin . ^^^ To determine , if ARF 6 selectively activated PLD 1 or PLD 2 , wild type and mutant forms of the ARF isoform were transfected together with PLD 1 or PLD 2 . ^^^ |
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| Interacting proteins: Q13393 and P62330 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13393 and P62330 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13393 and P62330 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13393 and P62330 |
Pubmed |
SVM Score :0.0 |
| NA |
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