| Interacting proteins: P62070 and P04049 |
Pubmed |
SVM Score :0.92038098 |
| Unexpectedly , yeast two hybrid binding analyses showed that although both Ras and TC 21 could interact with the isolated Ras binding domain of Raf 1 , only Ras interacted with full length Raf 1 , A Raf , or B Raf . 0.92038098^^^ |
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| Interacting proteins: P62070 and P04049 |
Pubmed |
SVM Score :0.0 |
| While activation of the serine / threonine kinases Raf 1 and B Raf has been implicated in signalling pathways leading to transformation by H Ras , it has been argued that TC 21 does not activate Raf 1 or B Raf . ^^^ Raf 1 and B Raf are constitutively active in TC 21 transformed cells and the ERK / MAPK cascade is required for the maintenance of the transformed state . ^^^ Furthermore , using point mutations in the effector loop of TC 21 , we show that the interaction of TC 21 with Raf 1 is crucial for transformation . . ^^^ |
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| Interacting proteins: P62070 and P04049 |
Pubmed |
SVM Score :0.0 |
| However , unlike Ras , TC 21 fails to bind to and activate the Raf 1 serine threonine kinase . ^^^ Thus , whereas Ras transformation is critically dependent on Raf 1 TC 21 activity is promoted by activation of Raf independent signaling pathways . ^^^ First we determined the basis for the inability of TC 21 to activate Raf 1 . ^^^ Whereas Ras can interact with the two distinct Ras binding sequences in NH 2 terminus of Raf 1 , designated RBS 1 and Raf Cys , TC 21 could only bind Raf Cys . ^^^ Thus , the inability of TC 21 to bind to RBS 1 may prevent it from promoting the translocation of Raf 1 to the plasma membrane . ^^^ |
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| Interacting proteins: P62070 and P04049 |
Pubmed |
SVM Score :0.0 |
| However , in contrast to Ras , we found previously that TC 21 fails to activate the Raf 1 serine / threonine kinase . ^^^ |
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| Interacting proteins: P62070 and P04049 |
Pubmed |
SVM Score :0.0 |
| When 14 3 3 binding to conserved region 2 ( CR 2 ) was disrupted , Raf 1 basal kinase activity was elevated and it could be further activated by ( V 12 , G 37 ) Ras , ( V 23 ) TC 21 , and ( V 38 ) R Ras . ^^^ |
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| Interacting proteins: P62070 and P04049 |
Pubmed |
SVM Score :0.0 |
| NA |
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