| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.50583817 |
| Nuclear transport factor 2 ( NTF 2 ) is associated with the translocation stage of nuclear protein import and binds both to nuclear pore proteins ( nucleoporins ) containing phenylalanine rich repeats and to the Ras family GTPase Ran . 0.50583817^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.57425256 |
| We have used a range of complementary biochemical and biophysical methods to investigate the interactions between nuclear transport factor 2 ( NTF 2 ) , the Ras family GTPase Ran , and XFXFG nucleoporin repeats that are crucial for nuclear trafficking . 0.57425256^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.76444777 |
| Bacterially expressed rat NTF 2 exists in solution as dimers and , when bound to Sepharose beads , is able to interact specifically with both the Ras like GTPase Ran , and the xFxFG repeat containing domains of nucleoporins p 62 ( vertebrate ) and Nsp1p ( yeast ) . 0.76444777^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.57950009 |
| This interaction between NTF 2 and GDP Ran is required for efficient nuclear protein import and may function between the docking and translocation steps of the pathway . . 0.57950009^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.79812487 |
| Engineered mutants in the switch 2 loop of Ran define the contribution made by key residues to the interaction with nuclear transport factor 2 ( NTF 2 ) and the role of this interaction in nuclear protein import . 0.79812487^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| Nuclear transport factor 2 ( NTF 2 ) facilitates protein transport into the nucleus and interacts with both the small Ras like GTPase Ran and nucleoporin p 62 . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| We demonstrate that nuclear transport factor 2 ( NTF 2 ) , nucleoporin p 62 and the Ras like GTPase Ran bind to the importin heterodimer via its beta subunit . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| Structural basis for molecular recognition between nuclear transport factor 2 ( NTF 2 ) and the GDP bound form of the Ras family GTPase Ran . ^^^ Nuclear transport factor 2 ( NTF 2 ) and the Ras family GTPase Ran are two soluble components of the nuclear protein import machinery . ^^^ We have used 10 ray crystallography to determine the structure of the macromolecular complex formed between GDP Ran and nuclear transport factor 2 ( NTF 2 ) at 2 . 5 A resolution . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| The addition of energy and nuclear transport factor 2 ( NTF 2 ) [ 5 ] was sufficient for the accumulation of Ran in the nucleus . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| In this system , export is dependent on exogenous CRM1 / Exportin1 a nuclear export receptor the GTPase Ran and nucleotide triphosphates ( NTPs ) , and is further stimulated by Ran binding protein 1 ( RanBP 1 ) and nuclear transport factor 2 ( NTF 2 ) . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| The mechanism of ran import into the nucleus by nuclear transport factor 2 . ^^^ We show that nuclear transport factor 2 ( NTF 2 ) is required to concentrate Ran in the nucleus in the budding yeast , Saccharomyces cerevisiae . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| One such carrier is nuclear transport factor 2 ( NTF 2 ) , whose import cargo is the small GTPase Ran . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| RESULTS : We found genetic interaction between genes establishing the Ran gradient , nuclear transport factor 2 ( ntf 2 ) , Ran GTPase activating protein ( Sd ) , and the gene encoding Drosophila Profilin , chickadee ( chic ) . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| Identification and characterization of the Arabidopsis orthologs of nuclear transport factor 2 , the nuclear import factor of ran . ^^^ Nuclear import of Ran relies on a small RanGDP binding protein , Nuclear Transport Factor 2 ( NTF 2 ) . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| The second step , translocation , requires at least two soluble factors , a small GTPase Ran and its interacting protein p10 / nuclear transport factor 2 ( NTF 2 ) , along with nuclear pore complex components . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| Overexpression of NTF 2 can suppress the temperature sensitive phenotype of gsp 1 1 and gsp 1 2 and partially rescue nuclear transport defects . ^^^ However , overexpression of a mutant allele of NTF 2 with decreased binding to Gsp1p can not rescue the temperature sensitivity of gsp 1 1 and gsp 1 2 . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| Nuclear import of proteins containing a nuclear localization signal ( NLS ) is dependent on the presence of a cytoplasmic NLS receptor , the GTPase Ran , and p10 / NTF 2 . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| Recombinant importin alpha , importin beta , Ran , and NTF 2 alone were sufficient to support the import of both L form and D form conjugates in permeabilized cells . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| NTF 2 mediates nuclear import of Ran . ^^^ A direct NTF 2 RanGDP interaction is crucial for this process , since point mutations that disturb the RanGDP NTF 2 interaction also interfere with Ran import . ^^^ The release of Ran from NTF 2 into the nucleus , and thus the directionality of Ran import , probably involves nucleotide exchange to generate RanGTP , for which NTF 2 has no detectable affinity , followed by binding of the RanGTP to an importin beta family transport receptor . . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| We report here isolation of three multicopy suppressors PDE 2 , NTF 2 , and a gene designated MOG 1 all of which rescued a growth defect of these gsp 1 strains . ^^^ Previously , NTF 2 was reported to suppress gsp 1 but not PDE 2 . ^^^ Both MOG 1 and NTF 2 suppressed a series of gsp 1 alleles with similar efficiency , and both suppressed gsp 1 even with a single gene dose . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| Nuclear import of PTHrP was assessed in vitro using purified components , demonstrating that importin beta , together with the GTP binding protein Ran , was able to mediate efficient nuclear accumulation in the absence of importin alpha , whereas the addition of nuclear transport factor NTF 2 reduced transport . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| NLS dependent nuclear import was reconstituted in vitro using the different importin subunits together with the transport factors Ran and NTF 2 . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| Identification of an NTF 2 related factor that binds Ran GTP and regulates nuclear protein export . ^^^ Ran function is modulated in a noncatalytic fashion by NTF 2 , a protein that mediates nuclear import of Ran GDP . ^^^ Here we characterize a novel component of the Ran system that is 26 % identical to NTF 2 , which based on its function we refer to as NTF 2 related export protein 1 ( NXT 1 ) . ^^^ In contrast to NTF 2 , NXT 1 preferentially binds Ran GTP , and it colocalizes with the nuclear pore complex ( NPC ) in mammalian cells . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| In rat liver nuclei , poly ( A ) ( + ) RNA , hnRNPs A 1 and C , mrnp 41 , ASF , and a phosphorylated subset of SR proteins were also distributed like mRNA , as were various transport factors and their cargoes ( NTF 2 , Ran , RCC 1 , karyopherin ( beta ) , Rch 1 , transportin ( alpha ) , m ( 2 , 2 , 7 ) trimethylG ) . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| Monoclonal antibodies to NTF 2 inhibit nuclear protein import by preventing nuclear translocation of the GTPase Ran . ^^^ NTF 2 has been shown to bind nuclear pore complex proteins and the GDP form of Ran in vitro . ^^^ Recently , it has been reported that NTF 2 can stimulate the accumulation of Ran in digitonin permeabilized cells . ^^^ Evidence that NTF 2 directly mediates Ran import or that NTF 2 is required to maintain the nuclear concentration of Ran in living cells has not been obtained . ^^^ Here we show that cytoplasmic injection of anti NTF 2 mAbs resulted in a dramatic relocalization of Ran to the cytoplasm . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| Proteins that regulate the GTPase cycle and subcellular distribution of Ran include the cytoplasmic GTPase activating protein ( RanGAP ) and its co factors ( RanBP 1 , RanBP 2 ) , the nuclear guanine nucleotide exchange factor ( RanGEF ) , and the Ran import receptor ( NTF 2 ) . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| The interaction between Ran and NTF 2 is required for cell cycle progression . ^^^ Here , we examine the in vivo role of NTF 2 in Ran import and the effect that disruption of Ran imported into the nucleus has on the cell cycle . ^^^ A temperature sensitive ( ts ) mutant of Saccharomyces cerevisiae NTF 2 that does not bind to Ran is unable to import Ran into the nucleus at the nonpermissive temperature . ^^^ These findings demonstrate that NTF 2 is required to transport Ran into the nucleus in vivo . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| While transport factors such as Ran and NTF 2 show only transient interaction with the pores , a number of soluble transport receptors , including importin beta , show a tight association with the purified pores . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| Of the nuclear transport proteins , karyopherin beta 1 , Rch 1 , Ran , and RCC 1 were detected at high concentrations ; NTF 2 , nucleoporin p 62 , and Hsp 70 were detected at low concentrations ; while tranportin , CAS , and Ran BPI were not detected . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| The interaction between NTF 2 and Ran is essential for the import of Ran into the nucleus . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| The TAP p 15 heterodimer is structurally similar to the homodimeric transport factor NTF 2 , but unlike NTF 2 , it is incompatible with either homodimerization or Ran binding . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| We describe the identification and characterization of the RanGTPase and its binding partners : the guanine nucleotide exchange factor , RanGEF ; the GTPase activating protein , RanGAP ; the soluble import and export receptors ; Ran binding domain ( RBD ) containing proteins ; and NTF 2 and related factors . . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| In contrast , a significant reduction in affinity of the RanGDP binding protein NTF 2 for Ran CoreGDP is observed , indicating that the switch regions have changed conformation in the Ran Core mutant . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| Engineered mutants at the interaction interface reduce the binding of NTF 2 to nuclear pores and cause reduced growth rates and Ran mislocalization when substituted for the wild type protein in yeast . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| Four mutant complementation groups were characterized that correspond to respective mutations in genes encoding Ran ( gsp 1 ) , and essential Ran regulatory factors Ran GTPase activating protein ( rna 1 ) , Ran guanine nucleotide exchange factor ( prp 20 ) , and the RanGDP import factor ( ntf 2 ) . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| CLIC 4 associates with the Ran , NTF 2 , and Importin alpha nuclear import complexes in immunoprecipitates of lysates from cells treated with apoptotic / stress inducing agents . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| Here , we identify a novel activity that stimulates release of GDP from Ran in the presence of NTF 2 . ^^^ In contrast , neither hydrolyzable ATP nor nonhydrolyzable ATP analogues affect GDP dissociation from Ran catalyzed by recombinant RCC 1 or inhibition of GDP dissociation from Ran by recombinant NTF 2 . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| The model reflects experimentally determined rates for cargo import and correctly predicts that import is limited principally by Impalpha and Ran , but is also sensitive to NTF 2 . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| Using an in vitro transport system , the nuclear import of Snail was reconstituted by importin ( karyopherin ) beta in the presence of Ran and NTF 2 . ^^^ |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61970 and P62826 |
Pubmed |
SVM Score :0.0 |
| NA |
|