| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.50590719 |
| Interestingly , DCoH retains its enzymatic activity while complexed with HNF 1 . 0.50590719^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| A dimerization cofactor of HNF 1 alpha ( DCoH ) was identified that displayed a restricted tissue distribution and did not bind to DNA , but , rather , selectively stabilized HNF 1 alpha dimers . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| The bifunctional protein pterin 4a carbinolamine dehydratase ( PCD ) / dimerization cofactor of HNF 1 ( DCoH ) is a cytoplasmic enzyme involved in the tetrahydrobiopterin regeneration and is found in complex with the transcription factor HNF 1 in liver cell nuclei . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| The bi functional protein dimerization cofactor of HNF 1 ( DCoH ) / pterin 4 alpha carbinolamine dehydratase ( PCD ) is found in liver cell nuclei bound to the transcription factor hepatocyte nuclear factor 1 ( HNF 1 ) as well as in the cytoplasm acting as an enzyme involved in the phenylalanine hydroxylation system . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| Two recent crystal structures of the tetrameric pterin 4 alpha carbinolamine dehydratase ( PCD ) / dimerization cofactor of HNF 1 ( DCoH ) protein provide fresh insights into how this multifunctional enzyme / transcriptional coactivator works . . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| It is also a dimerization cofactor of HNF 1 and therefore is believed to function as part of the hepatic gene transcription system . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| Human white blood cells and hair follicles are good sources of mRNA for the pterin carbinolamine dehydratase / dimerization cofactor of HNF 1 for mutation detection . ^^^ Pterin carbinolamine dehydratase / dimerization cofactor of HNF 1 ( PCD / DCoH ) is a protein that has a dual function . ^^^ In addition , it is the dimerization cofactor of HNF 1 that is able to activate the transcriptional activity of HNF 1 . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| The bifunctional protein DCoH ( dimerization cofactor of HNF 1 ) is a transcriptional coactivator of homeodomain proteins and a cytoplasmic enzyme . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| Characterization of expression of the gene for human pterin carbinolamine dehydratase / dimerization cofactor of HNF 1 . ^^^ Pterin carbinolamine dehydratase / dimerization cofactor of HNF 1 ( PCD / DCoH ) is a dual function protein . ^^^ In the nucleus , it functions as a dimerization cofactor of HNF 1 and increases the transcriptional activity of HNF 1 . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| Mutations in the genes for hepatocyte nuclear factor ( HNF ) 1alpha , 4alpha , 1beta , and 3beta ; the dimerization cofactor of HNF 1 ; and insulin promoter factor 1 are not common causes of early onset type 2 diabetes in Pima Indians . ^^^ RESEARCH DESIGN AND METHODS : The coding regions of the known MODY genes hepatocyte nuclear factor ( HNF ) 1alpha , HNF 4alpha , HNF 1beta , and insulin promoter factor 1 and the coding regions of two MODY candidate genes , HNF 3beta and the dimerization cofactor of HNF 1 , were sequenced in genomic DNA from Pima Indians . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| Several MODY 3 mutations target the HNF 1alpha dimerization domain ( HNF p 1 ) , which binds the coactivator , dimerization cofactor of HNF 1 ( DCoH ) . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| The N terminal dimerization domain of the transcriptional activator hepatocyte nuclear factor 1alpha ( HNF 1alpha ) is essential for DNA binding and association of the transcriptional coactivator , DCoH ( dimerization cofactor of HNF 1 ) . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| Studies of the variability of the hepatocyte nuclear factor 1beta ( HNF 1beta / TCF 2 ) and the dimerization cofactor of HNF 1 ( DcoH / PCBD ) genes in relation to type 2 diabetes mellitus and beta cell function . ^^^ To investigate whether mutations in HNF 1 are implicated in the pathogenesis of MODY or late onset diabetes with and without nephropathy in Danish Caucasians we examined the HNF 1beta ( TCF 2 ) and the dimerization cofactor of HNF 1 ( DCoH , PCBD ) genes for mutations in 11 MODY probands , 28 type 2 diabetic patients with nephropathy , and 46 type 2 diabetic patients with an impaired beta cell function by combined single strand conformation polymorphism ( SSCP ) and heteroduplex analysis . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| Transcription by HNF1alpha is stimulated by the bifunctional coactivator DCoH ( dimerization cofactor of HNF 1 ) . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| Functions attributed to erh include enhancement of pyrimidine biosynthesis , a role in cell cycle regulation , and repression of the tissue specific transcription factor HNF 1 ( hepatocyte nuclear factor 1 ) through binding the coactivator DCoH ( dimerization cofactor of HNF 1 ) . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| The property of stabilizing LFB 1 dimers is also shared by HeLa cells which produce a HeLa homolog of DCoH . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| This enzyme has the identical amino acid sequence to DCoH , the dimerization cofactor of the transcription regulator , HNF 1 alpha . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| In mammals , LFB 1 activity is modulated by DCoH , a cofactor that stimulates the LFB 1 transactivation significantly . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| Regulation of the HNF 1 homeodomain proteins by DCoH . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| The bifunctional protein PCD / DCoH is both an enzyme involved in the phenylalanine hydroxylation system and a transcription coactivator forming a 2 : 2 heterotetrameric complex with the nuclear transcription factor HNF 1 . ^^^ The crystal structures of the tetrameric rat and the dimeric bacterial PCD / DCoH have led to the proposal of substrate and HNF 1 binding sites . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| The cofactor DCoH , known to stabilize dimer formation of HNF 1 alpha , is present as a maternal factor in the egg and has a partially distinct tissue distribution compared to HNF 1 alpha . ^^^ This implies that DCoH does not only modulate HNF 1 alpha dimerization but may also cooperate with other transcription factors . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| DCoH , the dimerization cofactor of hepatocyte nuclear factor 1 ( HNF 1 ) , functions as both a transcriptional coactivator and a pterin dehydratase . ^^^ The dihedral symmetry of DCoH suggests that binding to the dimerization domain of HNF 1 likely involves the superposition of two fold rotation axes of the two proteins . . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| The bifunctional DCOH protein binds to HNF 1 independently of its 4 alpha carbinolamine dehydratase activity . ^^^ We repeatedly obtained cDNA clones encoding DCOH / 4 alpha carbinolamine dehydratase , an enzyme involved in the oxidation of aromatic amino acids that was shown to bind to and stabilise HNF 1 dimers . ^^^ Using the yeast system , we show that the enzymatic activity of DCOH is not essential for HNF 1 binding and that the HNF 1 dimerisation domain is sufficient for DCOH binding . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| The cofactor of the HNF 1 proteins ( DCoH ) is present throughout development and thus can not limit the activation potential of HNF 1 alpha in early development . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| DCoH dimers harbor a binding site for hepatocyte nuclear factor 1 ( HNF 1 ) , two active centers that bind pterins , and a saddle shaped surface that resembles nucleic acid binding domains . ^^^ The transcriptional function of DCoH therefore is composed of two parts , HNF 1 binding and another contributing effect that involves the active site and , indirectly , the saddle . . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| It is also identical to the dimerization cofactor of hepatocyte nuclear factor 1 ( HNF 1 ) ( DCoH ) that is able to enhance the transcriptional activity of HNF 1 . ^^^ However , this 9 kb fragment was transactivated by HNF 1 in a dose dependent manner with a maximum of nearly 8 fold activation ; DCoH potentiated this transactivation by another 1 . 6 fold . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| DCoH might have a function in tissue specific gene expression mediated by HNF 1 in the adults and in the developing embryo as it is found in the kidney and the liver , organs known to contain HNF 1 . ^^^ In addition DCoH is a maternal factor in the rat egg lacking HNF 1 transcription factors . ^^^ Evidence for a HNF 1 independent function of DCoH is also given by the fact that DCoH is present in the eyes ( pigmented epithelium ) and the brain ( ependym cells ) of the rat embryos , cell types lacking HNF 1 proteins . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| A second regulatory task of the same protein is DCoH , a coactivation of transcription by hepatocyte nuclear factor 1alpha ( HNF 1alpha ) , a function that is apparently not impaired in these HPA individuals . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| Three conserved histidines have been shown to be important for the enzymatic activity of 4a hydroxy tetrahydropterin dehydratase , a bifunctional enzyme which is involved in regeneration of tetrahydrobiopterin and is also a cofactor ( DCoH ) for the transcription factor HNF 1alpha . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| In addition to playing a catalytic role in pterin recycling in the cytoplasm , it plays a regulatory role in the nucleus , where it acts as a dimerization cofactor component ( called DCoH ) for the transcriptional activator HNF 1alpha . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| The bifunctional protein PCD / DCoH is both a pterin 4alpha carbinolamine dehydratase ( PCD ) involved in the recycling of tetrahydrobiopterin ( BH 4 ) and a dimerisation cofactor ( DCoH ) of the hepatic nuclear factor 1alpha ( HNF 1alpha ) . ^^^ Our results show that PCD / DCoH is present in cells expressing enzymes that use BH 4 as a cofactor and / or HNF 1alpha . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| Pterin 4a carbinolamine dehydratase ( PCD ) is a bifunctional protein also known as DCoH ( dimerization co factor of hepatocyte nuclear factor 1 ( HNF 1 ) ) . ^^^ PCD / DCoH modulates the DNA binding specificity of HNF 1 , thus acting on its transcriptional activity . ^^^ Immunoblotting and RT PCR analysis for PCD / DCoH and HNF 1 were also performed on both human tissues and CACO 2 and HT 29 cell lines . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| Ectopic pigmentation in Xenopus in response to DCoH / PCD , the cofactor of HNF 1 transcription factor / pterin 4alpha carbinolamine dehydratase . ^^^ DCoH , the dimerization cofactor of the HNF 1 homeodomain proteins ( hepatocyte nuclear factor 1alpha and beta ) , is involved in gene expression by associating with these transcription factors . ^^^ DCoH / PCD presumably cooperates with other partners , because it is expressed earlier than HNF 1 and phenylalanine hydroxylase ( PAH ) in early vertebrate development . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| DCoHm ( dimerization cofactor of hepatocyte nuclear factor 1alpha ( HNF1alpha ) from muscle ) , a novel gene of the DCoH family with 78 % amino acid identity to DCoH , was identified as a Mirk binding protein by yeast two hybrid analysis and cloned . ^^^ DCoH stabilizes HNF1alpha as a dimer and enhances its transcriptional activity on the beta fibrinogen promoter reporter , and DCoHm had similar activity . ^^^ Mirk may function as an HNF1alpha transcriptional activator in response to an MKK 3 mediated stress signal , and the selective expression of DCoH could restrict the Mirk response to carcinoma cells . . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| The bifunctional protein DCoH ( Dimerizing Cofactor for HNF 1 ) acts as an enzyme in intermediary metabolism and as a binding partner of the HNF 1 family of transcriptional activators . ^^^ DCoH acts as a cofactor for HNF 1 that stabilizes the dimeric HNF 1 complex . ^^^ Surprisingly , HNF 1 function in DCoH null mice is only slightly impaired , and mice are mildly glucose intolerant in contrast to HNF1alpha null mice , which are diabetic . ^^^ DCoH function as it pertains to HNF 1 activity appears to be partially complemented by a newly identified homolog , DCoH2 . . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| These were 92bp upstream of SLC3A1 , 52bp upstream of PCBD ( DCOH ) , and 42202bp upstream of TCF 2 ( HNF 1 beta ) . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| PCD also functions as a dimerization cofactor ( DCoH ) for the hepatocyte nuclear factor 1alpha ( HNF1alpha ) and the relative abundance of PCD / DCoH in the nucleus is also decreased . ^^^ HNF1alpha is required for the expression of PAH in the liver and our results support a role for PCD / DCoH , through its interaction with HNF1alpha , in regulating the expression of PAH . . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| DCoHalpha demonstrated the ability to quantitatively alter HNF 1 dependent DNA binding in vitro whereas DCoH was ineffective in vitro . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| The human pterin 4 alpha carbinolamine dehydratase ( PCD ) / dimerization cofactor for the transcription factor HNF 1 alpha is a bifunctional protein proposed to be involved in entirely different biochemical functions . ^^^ Characterization of the human PCBD gene encoding the bifunctional protein pterin 4 alpha carbinolamine dehydratase / dimerization cofactor for the transcription factor HNF 1 alpha . ^^^ |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61457 and P20823 |
Pubmed |
SVM Score :0.0 |
| NA |
|