Pubmed abstracts for Protein-Protein Interaction search result :


Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
Cloning of a novel somatostatin receptor , SSTR 3 , coupled to adenylylcyclase . ^^^ The pharmacological properties of mSSTR 3 and its ability to couple with adenylylcyclase distinguish SSTR 3 from the other cloned somatostatin receptors and indicates that it mediates biological functions different from SSTR 1 or SSTR 2 . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
Somatostatin receptors , an expanding gene family : cloning and functional characterization of human SSTR 3 , a protein coupled to adenylyl cyclase . ^^^ Membranes from cells expressing human SSTR 3 bound the somatostatin agonist [ 125I ] CGP 23996 specifically and with high affinity , with a rank order of potency of somatostatin 28 = CGP 23996 > somatostatin 14 > SMS 201 995 . ^^^ Studies using cells transiently coexpressing the human dopamine D 1 receptor and human SSTR 3 showed that somatostatin was able to inhibit dopamine stimulated cAMP formation in a dose dependent manner , indicating that SSTR 3 was functionally coupled to adenylyl cyclase . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
The expression of three somatostatin receptor subtypes , SSTR 3 , SSTR 4 , and SSTR 5 , was evaluated in 33 pituitary tumor specimens . ^^^ The widespread pituitary adenoma expression of SSTR 3 , regardless of hormonal secretory type , suggests that SSTR 3 might be involved in a somatostatin action ( s ) other than GH or TSH regulation . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
To test whether Asp 124 of the rat somatostatin receptor subtype 3 ( SSTR 3 ) is responsible for the binding of somatostain 14 ( SST 14 ) , this amino acid residue was replaced by an asparagine or a glutamic acid by polymerase chain reaction ( PCR ) mediated site directed mutagenesis . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
The latter was confirmed by reverse transcription PCR , which revealed the existence of the somatostatin receptor subtypes 2 and 3 ( SSTR 2 and SSTR 3 ) , with a relative mRNA concentration of 85 and 15 % , respectively . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
To determine the molecular mechanisms of action of somatostatin , we have investigated the interaction of the different cloned receptors with G proteins and cellular effector systems . sstr 2 , sstr 3 and sstr 5 associate with pertussis toxin sensitive G proteins and are able to mediate the inhibition of adenylyl cyclase activity by somatostatin . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
The human somatostatin receptors vary in size from 364 ( sstr 5 ) to 418 ( sstr 3 ) amino acids with 46 61 % amino acid identity between receptors , and 105 amino acids are invariant . ^^^ Somatostatin analogues selective for sstr 2 , sstr 3 and sstr 5 have been identified which will facilitate in vivo studies of the functions of these somatostatin receptors . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
Co expression of somatostatin SSTR 3 and SSTR 4 receptor messenger RNAs in the rat brain . ^^^ In situ hybridization histochemistry was used to study the distribution and possible co expression of the messenger RNA of the somatostatin receptor subtypes SSTR 4 and SSTR 3 in rat brain . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
Six SS 8 analogues that distinguish SS subtypes showed that 125I SS 14 bound to somatostatin receptor subtype 3 ( SSTR 3 ) . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
Studies by the reverse transcription polymerase chain reaction ( RT PCR ) showed that mRNA transcripts of the somatostatin receptor ( SSTR ) 2 , SSTR 3 and SSTR 4 genes were present in COR L 103 cells . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
Here , we report the distribution of somatostatin receptor 3 ( SSTR 3 ) mRNA in spinal cord and spinal ganglia by in situ hybridization . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
SST 14 ( 1 microgram ) , the SSTR 5 agonist , BIM 23052 , ( 0 . 5 1 microgram ) , SSTR 3 ( 0 . 5 micrograms ) and 2 ( 0 . 5 microgram ) or vehicle injected i . c . did not modify basal acid secretion . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
The somatostatin receptor agonists included peptides with relative specificity for SSTR 1 5 ( somatostatin 14 ; 10 nmol . kg 1 . h 1 ) ; SSTR 2 , SSTR 3 , and SSTR 5 [ SMS ( 201 995 ) ; 10 nmol . kg 1 . h 1 ] ; SSTR 2 ( 1 1 , 000 nmol . kg 1 . h 1 ) ; SSTR 3 ( 10 1 , 000 nmol . kg 1 . h 1 ) ; and SSTR 5 ( 10 1 , 000 nmol . kg 1 . h 1 ) . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
Whereas all of the octapeptides had much lower affinity for SSTR 1 and SSTR 3 receptors , which appear to be primarily present in the CNS , high affinity and highly specific ligands for the latter were found within a series of linear somatostatin analogs . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
Expression and localization of somatostatin receptor SSTR 1 , SSTR 2 , and SSTR 3 messenger RNAs in primary human tumors using in situ hybridization . ^^^ Somatostatin receptor gene expression of SSTR 1 , SSTR 2 , and SSTR 3 subtypes was evaluated by in situ hybridization in 55 human primary tumors shown to contain a high density of somatostatin receptors in binding assays . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
A human somatostatin receptor ( SSTR 3 ) , located on chromosome 22 , displays preferential affinity for somatostatin 14 like peptides . ^^^ We report here on the cloning of a human intronless gene encoding a member of the G protein linked somatostatin ( SST ) receptor subfamily , termed SSTR 3 . ^^^ Membranes prepared from COS 7 cells transiently expressing the human SSTR 3 gene bound [ 125I ] Leu 8 , D Trp 22 , Tyr 25 SST 28 in a saturable manner with high affinity ( approximately 200 pM ) and with rank order of potency ( D Trp 8 SST 14 > SST 14 > SMS 201 995 > SST 28 ) indicative of a somatostatin 14 selective receptor . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
In the present study , we have investigated the affinities of a battery of SRIF analogues to bind to SRIF receptor subtypes SSTR 1 ( cloned somatostatin complex ) , SSTR 2 , and SSTR 3 , as well as their abilities to inhibit the release of growth hormone from anterior pituitary cells in vitro . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
Localization of somatostatin ( SRIF ) SSTR 1 , SSTR 2 and SSTR 3 receptor mRNA in rat brain by in situ hybridization . ^^^ In situ hybridization histochemistry was performed to analyse the distribution of the messenger RNA ( mRNA ) of three putative somatostatin ( SRIF ) receptors in rat brain , using oligonucleotide probes derived from the cDNA coding for SSTR 1 , SSTR 2 , and SSTR 3 receptors . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
Differential expression of messenger RNAs for somatostatin receptor subtypes SSTR 1 , SSTR 2 and SSTR 3 in adult rat brain : analysis by RNA blotting and in situ hybridization histochemistry . ^^^ The messenger RNAs encoding three somatostatin receptor subtypes , SSTR 1 , SSTR 2 and SSTR 3 , were detected in rat by RNA blotting and in situ hybridization histochemistry to identify the sites of synthesis and expression of these somatostatin receptor subtypes . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
The tissue distribution of mRNA encoding five somatostatin receptor subtypes , SSTR 1 , SSTR 2 , SSTR 3 , SSTR 4 , and SSTR 5 , was determined in adult rat tissues by solution hybridization / nuclease protection analysis using sequence specific cRNA probes . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
The genes encoding three different human somatostatin receptor subtypes , designated SSTR 1 , SSTR 2 , and SSTR 3 , were mapped to chromosomes 14 , 17 , and 22 , respectively , by analyzing their segregation in a panel of reduced human hamster somatic cell hybrids . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
The somatostatin analogue , octreotide , binds with high affinity to the SSTR 2 and SSTR 5 subtype and with a low affinity to the SSTR 3 subtype . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
In order to investigate the gonadal regulation of pituitary somatostatin receptors ( SSTRs ) gene expression , we studied the existence of sexual dimorphism on SSTR 1 , SSTR 2 , and SSTR 3 mRNA levels in the rat anterior pituitary . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
The messenger RNA ( mRNA ) expression of somatostatin ( SRIF ) receptors SSTR 1 , SSTR 2 , SSTR 3 , SSTR 4 and SSTR 5 ( called sst 1 5 , now ) was studied in rat brain between embryonic day 17 ( E 17 ) and post natal day 5 ( P 5 ) by in situ hybridization histochemistry and compared to that of adult rats . sst 1 receptor mRNA expression was very low and restricted at E 17 , spread out at E 18 , to reach very high levels comparable to that of adult at P 5 ( e . g . in temia tecta , posteromedial cortical amygdaloid nucleus , subiculum ) . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
Only those somatostatin analogs that shared a high affinity for sstr 3 receptors elicited muscle contraction . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
Internalized SSTR 3 is cycled back to the plasma membrane while endocytosed rho Ala 1 SST 14 remains inside the cell . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
Peptide agonists with relatively high specificity for SSTR 1 5 ( somatostatin 14 ) , SSTR 2 ( MK 678 ) , SSTR 3 ( L 362823 ) , and SSTR 5 ( L 362855 ) were infused i . v . after nutrient stimulated gastric acid secretion and gastrin release with intraduodenal perfusions of 8 % peptone and after secretagogue stimulated acid secretion with gastrin ( 75 pmol kg 1 h 1 ) or histamine ( 20 micrograms kg 1 h 1 ) . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
SSTR 3 , a somatostatin ( SST ) receptor , is an adenylyl cyclase ( AC ) inhibiting receptor . ^^^ Cellular transfection of SSTR 3 and measurement of SST dependent AC activity through co transfected chimeric G alpha ( s ) revealed that SSTR 3 recognizes the C termini of G alpha ( i1 / 2 ) but not of G alpha ( o ) or G alpha ( z ) , and those of G alpha ( 14 ) and G alpha ( 16 ) , but not of G alpha ( q ) or G alpha ( 11 ) . ^^^ As predicted by the chimeric G alpha ( s ) , SST bound SSTR 3 stimulated polyphosphoinositide turnover only when G alpha ( 16 ) or G alpha ( 14 ) was co transfected . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
RNA blot analysis revealed that MIN 6 cells expressed only SSTR 3 among the five somatostatin receptors so far identified . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
Treatment of membranes from sstr 2 , sstr 3 , or sstr 4 expressing cells with somatostatin 14 plus guanyl 5 ' yl imidodiphosphate ( GMPPNP ) increased PTPase activity , and this stimulation was pertussis toxin sensitive . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
Agonist dependent internalization of the rat somatostatin receptor subtype 3 ( SSTR 3 ) requires four hydroxyl amino acids ( Ser 341 , Ser 346 , Ser 351 , and Thr 357 ) in the receptor C terminus ( Roth , A . , Kreienkamp , H . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
These findings are consistent with a role of SSTR 3 in the observed somatostatin mediated inhibition of acetylcholine release from myenteric neurons and of secretomotor neuron activity in the submucous plexus . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
Among the three cases with a true positive study , SSTR 2 , the SSTR subtype that preferentially binds to the somatostatin analogue octreotide , was detected in two , SSTR 5 was demonstrated in the three , and SSTR 3 was detected in one . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
This is in contrast to another somatostatin receptor subtype , SSTR 3 , which mediates rapidly desensitizing currents . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
We found that two of the five subtypes of SSTR ( SSTR 3 and SSTR 4 ) are expressed in skeletal muscle with a time course that correlates with the transient expression of SST in motoneurons . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
These analogs bind with high affinity to only two SST receptor ( SSTR ) subtypes ( 2 and 5 ) , display moderate affinity for SSTR 3 , and low affinity for SSTR 1 and 4 . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
Somatostatin and somatostatin analogs specific for SSTR 2 and / or SSTR 3 enhanced adhesion of T cells to fibronectin ( FN ) , and to a certain extent , also to collagen type 4 ( CIV ) and laminin ( LAM ) . ^^^ T lymphocytes express multiple SSTR and somatostatin may therefore regulate lymphocyte functions via distinct receptor subtypes as shown here for adhesion to extracellular matrix components ( ECM ) via SSTR 2 and SSTR 3 . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
Tc 99m depreotide is a new radiolabeled somatostatin analogue that shows high affinity for SSTR 2 , SSTR 3 , and SSTR 5 subtypes . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
Whereas KS Imm cells do not express somatostatin receptors ( SSTRs ) , endothelial cells express several SSTRs , in particular SSTR 3 . ^^^ Somatostatin significantly inhibited angiogenesis in vivo in the matrigel sponge assay ; this inhibition was mimicked by the SSTR 3 agonist L 796778 and reversed by the SSTR 3 antagonist BN 81658 , demonstrating involvement of SSTR 3 . ^^^ BN 81658 reversed somatostatin inhibition of cell proliferation , NO production , and MAPK activity , indicating that SSTR 3 activation is required for the effects of somatostatin in vitro . ^^^ In conclusion , we demonstrate that somatostatin is a powerful antitumor agent in vivo that inhibits tumor angiogenesis through SSTR 3 mediated inhibition of both eNOS and MAPK activities . . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
RT PCR analysis demonstrated that HUVECs expressed the somatostatin receptor subtype SSTR 3 . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
InIII DOTA NOC exhibited the following IC 50 values ( nM ) when studied in competition with [ 125I ] [ Leu 8 , d Trp 22 , Tyr 25 ] somatostatin 28 ( values for YIII DOTA NOC are shown in parentheses ) : sstr 2 , 2 . 9 + / 0 . 1 ( 3 . 3 + / 0 . 2 ) ; sstr 3 , 8 + / 2 ( 26 + / 1 . 9 ) ; sstr 5 , 11 . 2 + / 3 . 5 ( 10 . 4 + / 1 . 6 ) . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
CONCLUSION : The effect exerted by somatostatin and its analogues on HSCs may mainly depend on the expression of SSTR 2 , SSTR 3 and SSTR 5 . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
The effect of somatostatin and SSTR 3 on proliferation and apoptosis of gastric cancer cells . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
SST inhibited NO production stimulated by bFGF through SST receptor 1 ( SSTR 1 ) , SSTR 2 , and SSTR 3 and by CCK through SSTR 2 and SSTR 3 . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
We have previously shown that the somatostatin receptors SSTR 2 and SSTR 3 are present on the parasite egg and worms . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
This action has been considered to be generally mediated via six different specific somatostatin receptors ( sstr ) , sstr 1 , sstr2A , sstr2B , sstr 3 , sstr 4 , and sstr 5 . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
Conformational analysis of a potent SSTR 3 selective somatostatin analogue by NMR in water solution . ^^^ The three dimensional structure of a potent SSTR 3 selective analogue of somatostatin , cyclo ( 3 14 ) H Cys ( 3 ) Phe ( 6 ) Tyr ( 7 ) D Agl ( 8 ) ( N ( beta ) Me , 2 naphthoyl ) Lys ( 9 ) Thr ( 10 ) Phe ( 11 ) Cys ( 14 ) OH ( des AA ( 1 , 2 , 4 , 5 , 12 , 13 ) [ Tyr ( 7 ) , D Agl ( 8 ) ( N ( beta ) Me , 2 naphthoyl ) ] SRIF ) ( peptide 1 ) has been determined by ( 1 ) H NMR in water and molecular dynamics ( MD ) simulations . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
Somatostatin acting via G ( 1 ) 1 coupled sstr 3 receptor , DPDPE ( [ D Pen 2 , D Pen 5 ] enkephalin ; where Pen is penicillamine ) acting via G ( 1 ) 2 coupled delta opioid receptors , and cyclopentyl adenosine acting via G ( 1 ) 3 coupled adenosine A 1 receptors preferentially activated PI3K ( phosphoinositide 3 kinase ) and ILK ( integrin linked kinase ) , whereas ACh ( acetylcholine ) acting via G ( 1 ) 3 coupled M 2 receptors preferentially activated PI3K , Cdc 42 ( cell division cycle 42 ) / Rac1 , PAK 1 ( p 21 activated kinase 1 ) and p 38 MAPK ( mitogen activated protein kinase ) . ^^^
Interacting proteins: P32745 and P61278 Pubmed SVM Score :0.0
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Cloning of a novel somatostatin receptor , SSTR 3 , coupled to adenylylcyclase . ^^^ The pharmacological properties of mSSTR 3 and its ability to couple with adenylylcyclase distinguish SSTR 3 from the other cloned somatostatin receptors and indicates that it mediates biological functions different from SSTR 1 or SSTR 2 . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
Somatostatin receptors , an expanding gene family : cloning and functional characterization of human SSTR 3 , a protein coupled to adenylyl cyclase . ^^^ Membranes from cells expressing human SSTR 3 bound the somatostatin agonist [ 125I ] CGP 23996 specifically and with high affinity , with a rank order of potency of somatostatin 28 = CGP 23996 > somatostatin 14 > SMS 201 995 . ^^^ Studies using cells transiently coexpressing the human dopamine D 1 receptor and human SSTR 3 showed that somatostatin was able to inhibit dopamine stimulated cAMP formation in a dose dependent manner , indicating that SSTR 3 was functionally coupled to adenylyl cyclase . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
The expression of three somatostatin receptor subtypes , SSTR 3 , SSTR 4 , and SSTR 5 , was evaluated in 33 pituitary tumor specimens . ^^^ The widespread pituitary adenoma expression of SSTR 3 , regardless of hormonal secretory type , suggests that SSTR 3 might be involved in a somatostatin action ( s ) other than GH or TSH regulation . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
To test whether Asp 124 of the rat somatostatin receptor subtype 3 ( SSTR 3 ) is responsible for the binding of somatostain 14 ( SST 14 ) , this amino acid residue was replaced by an asparagine or a glutamic acid by polymerase chain reaction ( PCR ) mediated site directed mutagenesis . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
The latter was confirmed by reverse transcription PCR , which revealed the existence of the somatostatin receptor subtypes 2 and 3 ( SSTR 2 and SSTR 3 ) , with a relative mRNA concentration of 85 and 15 % , respectively . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
To determine the molecular mechanisms of action of somatostatin , we have investigated the interaction of the different cloned receptors with G proteins and cellular effector systems . sstr 2 , sstr 3 and sstr 5 associate with pertussis toxin sensitive G proteins and are able to mediate the inhibition of adenylyl cyclase activity by somatostatin . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
The human somatostatin receptors vary in size from 364 ( sstr 5 ) to 418 ( sstr 3 ) amino acids with 46 61 % amino acid identity between receptors , and 105 amino acids are invariant . ^^^ Somatostatin analogues selective for sstr 2 , sstr 3 and sstr 5 have been identified which will facilitate in vivo studies of the functions of these somatostatin receptors . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
Co expression of somatostatin SSTR 3 and SSTR 4 receptor messenger RNAs in the rat brain . ^^^ In situ hybridization histochemistry was used to study the distribution and possible co expression of the messenger RNA of the somatostatin receptor subtypes SSTR 4 and SSTR 3 in rat brain . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
Six SS 8 analogues that distinguish SS subtypes showed that 125I SS 14 bound to somatostatin receptor subtype 3 ( SSTR 3 ) . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
Studies by the reverse transcription polymerase chain reaction ( RT PCR ) showed that mRNA transcripts of the somatostatin receptor ( SSTR ) 2 , SSTR 3 and SSTR 4 genes were present in COR L 103 cells . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
Here , we report the distribution of somatostatin receptor 3 ( SSTR 3 ) mRNA in spinal cord and spinal ganglia by in situ hybridization . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
The somatostatin receptor agonists included peptides with relative specificity for SSTR 1 5 ( somatostatin 14 ; 10 nmol . kg 1 . h 1 ) ; SSTR 2 , SSTR 3 , and SSTR 5 [ SMS ( 201 995 ) ; 10 nmol . kg 1 . h 1 ] ; SSTR 2 ( 1 1 , 000 nmol . kg 1 . h 1 ) ; SSTR 3 ( 10 1 , 000 nmol . kg 1 . h 1 ) ; and SSTR 5 ( 10 1 , 000 nmol . kg 1 . h 1 ) . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
Whereas all of the octapeptides had much lower affinity for SSTR 1 and SSTR 3 receptors , which appear to be primarily present in the CNS , high affinity and highly specific ligands for the latter were found within a series of linear somatostatin analogs . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
Expression and localization of somatostatin receptor SSTR 1 , SSTR 2 , and SSTR 3 messenger RNAs in primary human tumors using in situ hybridization . ^^^ Somatostatin receptor gene expression of SSTR 1 , SSTR 2 , and SSTR 3 subtypes was evaluated by in situ hybridization in 55 human primary tumors shown to contain a high density of somatostatin receptors in binding assays . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
A human somatostatin receptor ( SSTR 3 ) , located on chromosome 22 , displays preferential affinity for somatostatin 14 like peptides . ^^^ We report here on the cloning of a human intronless gene encoding a member of the G protein linked somatostatin ( SST ) receptor subfamily , termed SSTR 3 . ^^^ Membranes prepared from COS 7 cells transiently expressing the human SSTR 3 gene bound [ 125I ] Leu 8 , D Trp 22 , Tyr 25 SST 28 in a saturable manner with high affinity ( approximately 200 pM ) and with rank order of potency ( D Trp 8 SST 14 > SST 14 > SMS 201 995 > SST 28 ) indicative of a somatostatin 14 selective receptor . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
In the present study , we have investigated the affinities of a battery of SRIF analogues to bind to SRIF receptor subtypes SSTR 1 ( cloned somatostatin complex ) , SSTR 2 , and SSTR 3 , as well as their abilities to inhibit the release of growth hormone from anterior pituitary cells in vitro . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
Localization of somatostatin ( SRIF ) SSTR 1 , SSTR 2 and SSTR 3 receptor mRNA in rat brain by in situ hybridization . ^^^ In situ hybridization histochemistry was performed to analyse the distribution of the messenger RNA ( mRNA ) of three putative somatostatin ( SRIF ) receptors in rat brain , using oligonucleotide probes derived from the cDNA coding for SSTR 1 , SSTR 2 , and SSTR 3 receptors . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
Differential expression of messenger RNAs for somatostatin receptor subtypes SSTR 1 , SSTR 2 and SSTR 3 in adult rat brain : analysis by RNA blotting and in situ hybridization histochemistry . ^^^ The messenger RNAs encoding three somatostatin receptor subtypes , SSTR 1 , SSTR 2 and SSTR 3 , were detected in rat by RNA blotting and in situ hybridization histochemistry to identify the sites of synthesis and expression of these somatostatin receptor subtypes . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
The tissue distribution of mRNA encoding five somatostatin receptor subtypes , SSTR 1 , SSTR 2 , SSTR 3 , SSTR 4 , and SSTR 5 , was determined in adult rat tissues by solution hybridization / nuclease protection analysis using sequence specific cRNA probes . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
The genes encoding three different human somatostatin receptor subtypes , designated SSTR 1 , SSTR 2 , and SSTR 3 , were mapped to chromosomes 14 , 17 , and 22 , respectively , by analyzing their segregation in a panel of reduced human hamster somatic cell hybrids . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
The somatostatin analogue , octreotide , binds with high affinity to the SSTR 2 and SSTR 5 subtype and with a low affinity to the SSTR 3 subtype . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
In order to investigate the gonadal regulation of pituitary somatostatin receptors ( SSTRs ) gene expression , we studied the existence of sexual dimorphism on SSTR 1 , SSTR 2 , and SSTR 3 mRNA levels in the rat anterior pituitary . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
The messenger RNA ( mRNA ) expression of somatostatin ( SRIF ) receptors SSTR 1 , SSTR 2 , SSTR 3 , SSTR 4 and SSTR 5 ( called sst 1 5 , now ) was studied in rat brain between embryonic day 17 ( E 17 ) and post natal day 5 ( P 5 ) by in situ hybridization histochemistry and compared to that of adult rats . sst 1 receptor mRNA expression was very low and restricted at E 17 , spread out at E 18 , to reach very high levels comparable to that of adult at P 5 ( e . g . in temia tecta , posteromedial cortical amygdaloid nucleus , subiculum ) . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
Only those somatostatin analogs that shared a high affinity for sstr 3 receptors elicited muscle contraction . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
Peptide agonists with relatively high specificity for SSTR 1 5 ( somatostatin 14 ) , SSTR 2 ( MK 678 ) , SSTR 3 ( L 362823 ) , and SSTR 5 ( L 362855 ) were infused i . v . after nutrient stimulated gastric acid secretion and gastrin release with intraduodenal perfusions of 8 % peptone and after secretagogue stimulated acid secretion with gastrin ( 75 pmol kg 1 h 1 ) or histamine ( 20 micrograms kg 1 h 1 ) . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
SSTR 3 , a somatostatin ( SST ) receptor , is an adenylyl cyclase ( AC ) inhibiting receptor . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
RNA blot analysis revealed that MIN 6 cells expressed only SSTR 3 among the five somatostatin receptors so far identified . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
Treatment of membranes from sstr 2 , sstr 3 , or sstr 4 expressing cells with somatostatin 14 plus guanyl 5 ' yl imidodiphosphate ( GMPPNP ) increased PTPase activity , and this stimulation was pertussis toxin sensitive . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
Agonist dependent internalization of the rat somatostatin receptor subtype 3 ( SSTR 3 ) requires four hydroxyl amino acids ( Ser 341 , Ser 346 , Ser 351 , and Thr 357 ) in the receptor C terminus ( Roth , A . , Kreienkamp , H . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
These findings are consistent with a role of SSTR 3 in the observed somatostatin mediated inhibition of acetylcholine release from myenteric neurons and of secretomotor neuron activity in the submucous plexus . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
Among the three cases with a true positive study , SSTR 2 , the SSTR subtype that preferentially binds to the somatostatin analogue octreotide , was detected in two , SSTR 5 was demonstrated in the three , and SSTR 3 was detected in one . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
This is in contrast to another somatostatin receptor subtype , SSTR 3 , which mediates rapidly desensitizing currents . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
Somatostatin and somatostatin analogs specific for SSTR 2 and / or SSTR 3 enhanced adhesion of T cells to fibronectin ( FN ) , and to a certain extent , also to collagen type 4 ( CIV ) and laminin ( LAM ) . ^^^ T lymphocytes express multiple SSTR and somatostatin may therefore regulate lymphocyte functions via distinct receptor subtypes as shown here for adhesion to extracellular matrix components ( ECM ) via SSTR 2 and SSTR 3 . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
Tc 99m depreotide is a new radiolabeled somatostatin analogue that shows high affinity for SSTR 2 , SSTR 3 , and SSTR 5 subtypes . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
Whereas KS Imm cells do not express somatostatin receptors ( SSTRs ) , endothelial cells express several SSTRs , in particular SSTR 3 . ^^^ Somatostatin significantly inhibited angiogenesis in vivo in the matrigel sponge assay ; this inhibition was mimicked by the SSTR 3 agonist L 796778 and reversed by the SSTR 3 antagonist BN 81658 , demonstrating involvement of SSTR 3 . ^^^ BN 81658 reversed somatostatin inhibition of cell proliferation , NO production , and MAPK activity , indicating that SSTR 3 activation is required for the effects of somatostatin in vitro . ^^^ In conclusion , we demonstrate that somatostatin is a powerful antitumor agent in vivo that inhibits tumor angiogenesis through SSTR 3 mediated inhibition of both eNOS and MAPK activities . . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
RT PCR analysis demonstrated that HUVECs expressed the somatostatin receptor subtype SSTR 3 . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
InIII DOTA NOC exhibited the following IC 50 values ( nM ) when studied in competition with [ 125I ] [ Leu 8 , d Trp 22 , Tyr 25 ] somatostatin 28 ( values for YIII DOTA NOC are shown in parentheses ) : sstr 2 , 2 . 9 + / 0 . 1 ( 3 . 3 + / 0 . 2 ) ; sstr 3 , 8 + / 2 ( 26 + / 1 . 9 ) ; sstr 5 , 11 . 2 + / 3 . 5 ( 10 . 4 + / 1 . 6 ) . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
CONCLUSION : The effect exerted by somatostatin and its analogues on HSCs may mainly depend on the expression of SSTR 2 , SSTR 3 and SSTR 5 . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
The effect of somatostatin and SSTR 3 on proliferation and apoptosis of gastric cancer cells . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
We have previously shown that the somatostatin receptors SSTR 2 and SSTR 3 are present on the parasite egg and worms . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
This action has been considered to be generally mediated via six different specific somatostatin receptors ( sstr ) , sstr 1 , sstr2A , sstr2B , sstr 3 , sstr 4 , and sstr 5 . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
Conformational analysis of a potent SSTR 3 selective somatostatin analogue by NMR in water solution . ^^^ The three dimensional structure of a potent SSTR 3 selective analogue of somatostatin , cyclo ( 3 14 ) H Cys ( 3 ) Phe ( 6 ) Tyr ( 7 ) D Agl ( 8 ) ( N ( beta ) Me , 2 naphthoyl ) Lys ( 9 ) Thr ( 10 ) Phe ( 11 ) Cys ( 14 ) OH ( des AA ( 1 , 2 , 4 , 5 , 12 , 13 ) [ Tyr ( 7 ) , D Agl ( 8 ) ( N ( beta ) Me , 2 naphthoyl ) ] SRIF ) ( peptide 1 ) has been determined by ( 1 ) H NMR in water and molecular dynamics ( MD ) simulations . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
Somatostatin acting via G ( 1 ) 1 coupled sstr 3 receptor , DPDPE ( [ D Pen 2 , D Pen 5 ] enkephalin ; where Pen is penicillamine ) acting via G ( 1 ) 2 coupled delta opioid receptors , and cyclopentyl adenosine acting via G ( 1 ) 3 coupled adenosine A 1 receptors preferentially activated PI3K ( phosphoinositide 3 kinase ) and ILK ( integrin linked kinase ) , whereas ACh ( acetylcholine ) acting via G ( 1 ) 3 coupled M 2 receptors preferentially activated PI3K , Cdc 42 ( cell division cycle 42 ) / Rac1 , PAK 1 ( p 21 activated kinase 1 ) and p 38 MAPK ( mitogen activated protein kinase ) . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
SST 14 ( 1 microgram ) , the SSTR 5 agonist , BIM 23052 , ( 0 . 5 1 microgram ) , SSTR 3 ( 0 . 5 micrograms ) and 2 ( 0 . 5 microgram ) or vehicle injected i . c . did not modify basal acid secretion . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
Internalized SSTR 3 is cycled back to the plasma membrane while endocytosed rho Ala 1 SST 14 remains inside the cell . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
We found that two of the five subtypes of SSTR ( SSTR 3 and SSTR 4 ) are expressed in skeletal muscle with a time course that correlates with the transient expression of SST in motoneurons . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
These analogs bind with high affinity to only two SST receptor ( SSTR ) subtypes ( 2 and 5 ) , display moderate affinity for SSTR 3 , and low affinity for SSTR 1 and 4 . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
SST inhibited NO production stimulated by bFGF through SST receptor 1 ( SSTR 1 ) , SSTR 2 , and SSTR 3 and by CCK through SSTR 2 and SSTR 3 . ^^^
Interacting proteins: P61278 and P32745 Pubmed SVM Score :0.0
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