| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| To investigate Na+ regulation of agonist binding to somatostatin receptors , Na+ was tested for its effect on the binding of agonists to cloned somatostatin receptor type 1 ( SSTR 1 ) and somatostatin receptor type 2 ( SSTR 2 ) stably expressed in Chinese hamster ovary cells . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Rat somatostatin receptor type 1 couples to G proteins and inhibition of cyclic AMP accumulation . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Agonist dependent up regulation of human somatostatin receptor type 1 requires molecular signals in the cytoplasmic C tail . ^^^ We have previously reported that the human somatostatin receptor type 1 ( hSSTR 1 ) stably expressed in Chinese hamster ovary K 1 cells does not internalize but instead up regulates at the membrane during continued agonist treatment ( 1 microM somatostatin ( SST ) 14 10 22 h ) . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Somatostatin receptor type 1 , 2 , 3 , and 5 mRNA was detected only in tumoral tissue . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Somatostatin receptor type 1 was modelled based on the atomic structure of bovine rhodopsin . ^^^ Possible ways of binding interaction between somatostatin receptor type 1 and TT 232 , a cycloheptapeptide analogue of somatostatin with broad therapeutic potential , were analysed by molecular docking . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Peptide chelating agent conjugate for selective targeting of somatostatin receptor type 1 : synthesis and characterization . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| The effects of somatostatin were insensitive to tetrodotoxin , reduced by Ba2+ , occluded or abolished by the presence of nonhydrolysable GTP or GDP analogues , respectively , and blocked or mimicked by a somatostatin receptor type 2 antagonist ( BIM 23627 ) or somatostatin receptor type 2 agonist ( L 779 , 976 ) , respectively , while somatostatin receptor type 1 , 3 and 4 agonists were ineffective ( L 797 , 591 , L 796 , 778 , L 803 , 087 ) . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Agonist induced up regulation of human somatostatin receptor type 1 is regulated by beta arrestin 1 and requires an essential serine residue in the receptor C tail . ^^^ We have previously shown that the human somatostatin receptor type 1 ( hSSTR 1 ) does not undergo agonist induced internalization , but is instead up regulated at the membrane upon prolonged somatostatin ( SST ) exposure . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Somatostatin receptor type 1 expression was significantly decreased , whereas type 4 receptor was expressed , which are unusual characteristics for pituitary tumors . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Previous studies have shown that at least two subtypes of somatostatin ( SRIF ) receptors ( SRIF 1 and SRIF 2 ) are expressed in mammalian cells . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Two classes of somatostatin receptors , SRIF 1 and SRIF 2 , have been distinguished biochemically and pharmacologically . ^^^ The pharmacological characteristics of receptors expressing these cDNAs resemble those of the SRIF 2 and SRIF 1 classes of somatostatin receptors , respectively . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Pharmacological characterisation of human cerebral cortex somatostatin SRIF 1 and SRIF 2 receptors . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Autoradiographic analysis of somatostatin SRIF 1 and SRIF 2 receptors in the human brain and pituitary . ^^^ The distribution of somatostatin ( SRIF ) receptor sites was studied by in vitro receptor autoradiography in the human brain and pituitary using the SRIF 1 ( sst 2 ) receptor selective [ 125I ] Tyr 3 octreotide , the non subtype selective [ 125I ] LTT SRIF 28 ( [ Leu 8 , D Trp 22 , 125I Tyr 25 ] SRIF 28 ) and the SRIF 2 receptor selective [ 125I ] CGP 23996 ( c [ Asu Lys Asn Phe Phe Trp Lys Thr Tyr Thr Ser ] ) in buffer containing 120 mM Na+ . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Somatostatin ( SRIF ) receptor subtypes ( sst ) were characterized in hypothalamic neurons and astrocytes by quantitative reverse transcription polymerase chain reaction and radioreceptor assays using [ 125I Tyr 0 , D Trp 8 ] SRIF 14 as a ligand in ionic conditions discriminating between SRIF 1 ( sst 2 , 3 , and 5 receptors ) and SRIF 2 ( sst 1 and 4 receptors ) binding sites . ^^^ Somatostatin ( SRIF ) receptor subtypes ( sst ) were characterized in hypothalamic neurons and astrocytes by quantitative reverse transcription polymerase chain reaction and radioreceptor assays using [ 125I Tyr 0 , D Trp 8 ] SRIF 14 as a ligand in ionic conditions discriminating between SRIF 1 ( sst 2 , 3 , and 5 receptors ) and SRIF 2 ( sst 1 and 4 receptors ) binding sites . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| The pharmacological properties of mSSTR 3 and its ability to couple with adenylylcyclase distinguish SSTR 3 from the other cloned somatostatin receptors and indicates that it mediates biological functions different from SSTR 1 or SSTR 2 . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| We previously reported the cloning of two distinct somatostatin receptor ( SSTR ) subtypes , SSTR 1 and SSTR 2 . ^^^ Although both SSTR 1 and SSTR 2 bound somatostatin specifically and with high affinity , neither was coupled to adenylyl cyclase , a major cellular effector of somatostatin ' s actions . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Stably transfected Chinese hamster ovary cells expressing SSTR 1 or SSTR 2 exhibit specific somatostatin binding , with an apparently higher affinity for somatostatin 14 than somatostatin 28 , and NH 2 terminally extended form of somatostatin 14 . ^^^ A SSTR 1 probe hybridized to multiple DNA fragments in EcoRI digests of human and mouse DNA , indicating that SSTR 1 and SSTR 2 are members of a larger family of somatostatin receptors . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| The amino acid sequence of rSSTR 4 is 60 % and 48 % identical to that of somatostatin receptors SSTR 1 and SSTR 2 , respectively , two recently cloned subtypes . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| The genes encoding two different somatostatin receptors ( SSTRs ) have been isolated and characterized , and RNA blotting studies have shown that both SSTR 1 and SSTR 2 are expressed in the brain . ^^^ In order to gain a better understanding of the functions of somatostatin in the CNS , the distribution of SSTR 1 and SSTR 2 mRNAs was determined using the technique of in situ hybridization . ^^^ Several regions of the brain reported to contain dense somatostatin like immunoreactive terminal fields and receptor binding sites were devoid of both SSTR 1 and SSTR 2 mRNA , suggesting the existence of additional SSTR subtypes . . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| In radioligand binding studies we have compared the binding properties of sstr 1 5 with their activities as somatostatin receptors . ^^^ The similarities in receptor sequence and in the binding profiles of short synthetic somatostastin analogues such as octreotide , MK 678 or RC 160 for sstr 1 5 indicate the existence of two classes of receptors sstr1 / sstr4 with virtually no or very low affinity and sstr2 / sstr3 / sstr5 with intermediate to high affinity for the short somatostatin analogues . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| In 1992 , a major breakthrough in our understanding of somatostatin functions was made with the cloning of five different receptor genes ( sstr 1 to sstr 5 ) which belong to the seven transmembrane domain receptor family . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Multiple effector coupling of somatostatin receptor subtype SSTR 1 . ^^^ The signal transduction pathways of a cloned human somatostatin receptor subtype , SSTR 1 , have been investigated in CHO cells stably expressing this receptor . ^^^ In SSTR 1 expressing CHO cells , somatostatin 14 inhibits forskolin stimulated cAMP formation in a dose dependent manner with an ED 50 of 1 . 0 10 10 ( 9 ) M . ^^^ Somatostatin 14 inhibitory action on adenylyl cyclase and stimulatory action on inositol 1 , 4 , 5 trisphosphate formation are both blocked by pertussis toxin , indicating that these effects of SSTR 1 are mediated by pertussis toxin sensitive G protein ( s ) . ^^^ Antiserum against Gi alpha 3 blocked the inhibitory effects of somatostatin 14 on forskolin stimulated adenylyl cyclase , but antiserum against Gi alpha 1 / Gi alpha 2 did not , indicating that Gi alpha 3 dominantly couples SSTR 1 to adenylyl cyclase . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| This implies that SST analogs like SMS 201 995 , known to interact with SSTR2A , but not with SSTR 1 , act on pituitary tumors mainly via the SSTR 2 subtype . . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| In rat GH 3 pituitary tumour cells exposed to 1 microM somatostatin for up to 48 h , sstr 1 , 3 , 4 and 5 mRNA increased dramatically while sstr 2 mRNA exhibited a biphasic response . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| With the aim of elucidating the role of individual somatostatin receptors in the central control of growth hormone secretion , we have examined the distribution of sstr 1 and sstr 2 mRNAs in the hypothalamus of the adult rat by in situ hybridization using 35S labelled antisense riboprobes . ^^^ Within the arcuate nucleus , the distribution of cells expressing sstr 1 and sstr 2 was comparable to that of neurons which bind somatostatin 14 selectively , one third of which have been documented to contain growth hormone releasing hormone . ^^^ Within the periventricular nucleus , the distribution of cells expressing sstr 1 and , to a lesser extent , sstr 2 was reminiscent of that of both [ 125I ] somatostatin labelled and somatostatin immunoreactive cells . ^^^ Taken together , these results imply a role for both sstr 1 and sstr 2 receptors in the central regulation of growth hormone releasing hormone and somatostatin secretion , and hence of growth hormone release , by somatostatin . . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Pharmacological identity between somatostatin SS 2 binding sites and SSTR 1 receptors . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Finally , we studied the expression of the somatostatin receptor gene by both ribonuclease ( RNase ) protection and nuclear run on assays using cDNA encoding for two subtypes of the somatostatin receptor gene ( i . e . , SSTR 1 and SSTR 2 ) . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| It appears from this study that the rat lateral cerebellar nuclei contain a pure population of receptors exhibiting the same binding characteristics as the recently cloned sstr 1 somatostatin receptor . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Patterns of expression of SSTR 1 and SSTR 2 somatostatin receptor subtypes in the hypothalamus of the adult rat : relationship to neuroendocrine function . ^^^ Taken together , these distributional patterns conform to those of somatostatin binding sites as visualized by in vitro autoradiography , suggesting that an important proportion of SSTR 1 and SSTR 2 receptors in the hypothalamus are associated with the perikarya and dendrites of intrinsic neurons . ^^^ The distribution of SSTR 1 expressing cells within the periventricular , paraventricular and suprachiasmatic nuclei was similar to that of neurons previously reported to contain and / or express somatostatin in the brain suggesting that some of the SSTR 1 receptors may correspond to autoreceptors . ^^^ Within the arcuate nucleus , the distribution of SSTR 1 and SSTR 2 messenger RNA expressing cells was comparable to that of neurons previously found to selectively bind somatostatin 14 within this area . ^^^ Taken together , the present results suggest that SSTR 1 and SSTR 2 somatostatin receptor messenger RNAs are heavily expressed in those neurons containing somatostatin and / or growth hormone releasing factor and thereby imply a role for both SSTR 1 and SSTR 2 somatostatin receptor subtypes in neuroendocrine regulation of growth hormone secretion in both sexes of this species . . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Five SST receptors have been characterized ( SSTR 1 to 5 ) . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| The somatostatin receptor SSTR 1 is coupled to phosphotyrosine phosphatase activity in CHO K 1 cells . ^^^ To determine whether cloned somatostatin receptors could mediate coupling to phosphotyrosine phosphotyrosine phosphatase activity , we examined phosphatase activity after somatotostatin activation of the rat somatostatin receptors SSTR 1 and SSTR 2 after their stable expression in heterologous Chinese Hamster Ovary ( CHO K 1 ) cells . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Effects of the stable somatostatin analogue RC 160 on cell proliferation , tyrosine phosphatase activity , and intracellular calcium concentration were investigated in CHO cells expressing the five somatostatin receptor subtypes SSTR 1 to 5 . ^^^ Binding experiments were performed on crude membranes by using [ 125I labeled Tyr 11 ] somatostatin 14 ; RC 160 exhibited moderate to high affinities for SSTR 2 , 3 , and 5 ( IC 50 , 0 . 17 , 0 . 1 and 21 nM , respectively ) and low affinity for SSTR 1 and 4 ( IC 50 , 200 and 620 nM , respectively ) . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Human somatostatin receptor subtypes ( SSTR 1 5 ) bind their natural ligands SRIF 14 and SRIF 28 with high affinity . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Stimulation of tyrosine phosphatase and inhibition of cell proliferation by somatostatin analogues : mediation by human somatostatin receptor subtypes SSTR 1 and SSTR 2 . ^^^ The effects of somatostatin analogues RC 160 and SMS 201 995 on tyrosine phosphatase and cell proliferation were investigated in COS 7 and NIH 3T3 cells expressing human somatostatin receptor subtype 1 or 2 ( SSTR 1 or SSTR 2 ) . ^^^ The somatostatin analogues RC 160 and SMS 201 995 exhibited low affinity for SSTR 1 ( IC 50 of 0 . 43 and 1 . 5 microM , respectively ) and high affinity for SSTR 2 ( IC 50 of 0 . 27 and 0 . 19 nM ) . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| COS 7 cells were transfected with human somatostatin ( SRIF ) receptor type 1 and 2 ( human SSTR 1 and SSTR 2 , respectively ) cDNAs . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| A large number of somatostatin analogs taken from several major families of peptides has been examined for binding to three newly discovered somatostatin receptors ( SSTR 1 , 2 and 3 ) transfected and expressed in various cell membrane preparations . ^^^ Whereas all of the octapeptides had much lower affinity for SSTR 1 and SSTR 3 receptors , which appear to be primarily present in the CNS , high affinity and highly specific ligands for the latter were found within a series of linear somatostatin analogs . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Gene expression of somatostatin receptor subtypes , SSTR 1 and SSTR 2 , in human lung cancer cell lines . ^^^ In the present study , we investigated the expression of somatostatin receptor ( SSTR ) subtype ( SSTR 1 and SSTR 2 ) mRNA in various human lung cancer cell lines by the sensitive reverse transcription PCR method and Southern blotting . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Expression and localization of somatostatin receptor SSTR 1 , SSTR 2 , and SSTR 3 messenger RNAs in primary human tumors using in situ hybridization . ^^^ Somatostatin receptor gene expression of SSTR 1 , SSTR 2 , and SSTR 3 subtypes was evaluated by in situ hybridization in 55 human primary tumors shown to contain a high density of somatostatin receptors in binding assays . ^^^ The cases having SSTR 1 mRNA were identified in binding experiments with 125I labeled somatostatin 14 and 28 analogues rather than with 125I [ Tyr 3 ] octreotide . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| In the present study , we have investigated the affinities of a battery of SRIF analogues to bind to SRIF receptor subtypes SSTR 1 ( cloned somatostatin complex ) , SSTR 2 , and SSTR 3 , as well as their abilities to inhibit the release of growth hormone from anterior pituitary cells in vitro . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| The expression of two somatostatin receptor subtypes , SSTR 1 and SSTR 2 , was studied in 27 pituitary tumors and 1 chronic lymphocytic leukemia pituitary infiltrate . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| We recently have cloned five human somatostatin receptor subtypes ( SSTR 1 SSTR5 ) . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Subtype specific signaling mechanisms of somatostatin receptors SSTR 1 and SSTR 2 . ^^^ We expressed two somatostatin receptor subtypes , SSTR 1 and SSTR 2 , stably in mouse fibroblast Ltk cells and transiently in human embryonic kidney HEK 293 cells to investigate subtype specific pharmacological and functional properties . ^^^ The effects of GTP gamma S and pertussis toxin on [ 125I Tyr 11 ] somatostatin 14 binding indicated that SSTR 2 may couple exclusively to pertussis toxin sensitive G proteins , whereas SSTR 1 may couple to both pertussis sensitive and insensitive G proteins . ^^^ In contrast , only SSTR 1 mediated somatostatin inhibition of Na ( + ) H+ exchange activity , and this action was insensitive to pertussis toxin . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| We found that somatostatin inhibited cAMP accumulation in human embryonic kidney 293 cells only when cells were transfected with either SSTR 1 or SSTR 2 . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Localization of somatostatin ( SRIF ) SSTR 1 , SSTR 2 and SSTR 3 receptor mRNA in rat brain by in situ hybridization . ^^^ In situ hybridization histochemistry was performed to analyse the distribution of the messenger RNA ( mRNA ) of three putative somatostatin ( SRIF ) receptors in rat brain , using oligonucleotide probes derived from the cDNA coding for SSTR 1 , SSTR 2 , and SSTR 3 receptors . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Differential expression of messenger RNAs for somatostatin receptor subtypes SSTR 1 , SSTR 2 and SSTR 3 in adult rat brain : analysis by RNA blotting and in situ hybridization histochemistry . ^^^ The messenger RNAs encoding three somatostatin receptor subtypes , SSTR 1 , SSTR 2 and SSTR 3 , were detected in rat by RNA blotting and in situ hybridization histochemistry to identify the sites of synthesis and expression of these somatostatin receptor subtypes . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| The genes encoding three different human somatostatin receptor subtypes , designated SSTR 1 , SSTR 2 , and SSTR 3 , were mapped to chromosomes 14 , 17 , and 22 , respectively , by analyzing their segregation in a panel of reduced human hamster somatic cell hybrids . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Five seven transmembrane domain somatostatin receptor subtypes ( SSTR 1 5 ) have been recently cloned and only SSTR 1 has been shown to be present in the human stomach . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| To increase further the reliability and prognostic value of sstr identification , we investigated the presence of mRNA for the subtypes sstr 1 and sstr 2 by in situ hybridization on tumor specimen from 25 carcinoid patients ( 22 midgut , 2 foregut , and 1 hindgut ) , all receiving somatostatin analogue treatment ( 12 lanreotide , 8 octreotide , and 5 octastatin ) and compared this to the therapeutic response evaluated as inhibition of hormone secretion . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| The effects of octreotide , a long acting somatostatin agonist selective of the sstr2 / sstr3 / sstr5 receptor subtypes , on ectopic GH secretion and tumor growth were investigated in Wistar Furth female rats implanted with GH secreting ( GC ) cells which express mostly somatostatin receptors of the sstr 1 and sstr 2 subtypes . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| In order to investigate the gonadal regulation of pituitary somatostatin receptors ( SSTRs ) gene expression , we studied the existence of sexual dimorphism on SSTR 1 , SSTR 2 , and SSTR 3 mRNA levels in the rat anterior pituitary . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Development of a selective agonist at the somatostatin receptor subtype sstr 1 . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| The messenger RNA ( mRNA ) expression of somatostatin ( SRIF ) receptors SSTR 1 , SSTR 2 , SSTR 3 , SSTR 4 and SSTR 5 ( called sst 1 5 , now ) was studied in rat brain between embryonic day 17 ( E 17 ) and post natal day 5 ( P 5 ) by in situ hybridization histochemistry and compared to that of adult rats . sst 1 receptor mRNA expression was very low and restricted at E 17 , spread out at E 18 , to reach very high levels comparable to that of adult at P 5 ( e . g . in temia tecta , posteromedial cortical amygdaloid nucleus , subiculum ) . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| A review of prior data on other receptors suggests that this receptor may belong to a unique class of seven transmembrane receptors ( including somatostatin SSTR 1 , dopamine D 3 , and frizzled protein Fz ) for which G protein coupling has not been demonstrated . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| The tissue distribution of mRNA encoding five somatostatin receptor subtypes , SSTR 1 , SSTR 2 , SSTR 3 , SSTR 4 , and SSTR 5 , was determined in adult rat tissues by solution hybridization / nuclease protection analysis using sequence specific cRNA probes . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Among members of the G protein coupled receptor superfamily , the sequences of both msl 1 and msl 1 were most closely related to those of the somatostatin receptors ( SSTRs ) , having approximately 35 % identity with the sequence of SSTR 1 . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| The mRNA distribution in the brain and the coupling to cellular effector systems of four somatostatin receptors ( SSTR 1 4 ) was studied . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Both overlapping and distinct signaling pathways for somatostatin receptor subtypes SSTR 1 and SSTR 2 in pituitary cells . ^^^ To elucidate the signaling events mediated by specific somatostatin receptor ( SSTR ) subtypes , we expressed SSTR 1 and SSTR 2 individually in rat pituitary GH12C1 and F4C1 cells , which lack endogenous somatostatin receptors . ^^^ In transfected F4C1 cells , both SSTR 1 and SSTR 2 mediated somatostatin induced inhibition of adenylyl cyclase via a PTx sensitive pathway . ^^^ Thus , by expressing individual somatostatin receptor subtypes in pituitary cells , we have identified both overlapping and distinct signaling pathways for SSTR 1 and SSTR 2 , and have shown that sequences other than simply the intracellular domains are required for SSTR 2 to couple to the PLC signaling pathway . . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| To illustrate the broad applicability of this technique , we have presented data from cell lines expressing a glycoprotein hormone receptor for follicle stimulating hormone ( FSHR ) , CXC ( CXCR 2 ) , and CC chemokine ( CCR 1 ) receptors and peptide receptors from the somatostatin ( SSTR 1 , 2 , 5 ) and neuropeptide Y ( NPY Y 2 , Y 4 Rs ) families . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Recent molecular studies have revealed the existence of up to five membrane somatostatin receptor subtypes termed SSTR 1 5 . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Expression of the five somatostatin receptor ( SSTR 1 5 ) subtypes in rat pituitary somatotrophes : quantitative analysis by double layer immunofluorescence confocal microscopy . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| We analyzed the mRNA expression of somatostatin receptor subtypes 1 to 5 ( SSTR 1 5 ) in human lymphoid cell lines , human peripheral blood lymphocytes ( PBL ) , and human lymphatic leukemia cells , using the reverse transcription polymerase chain reaction method . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| The five different rat somatostatin receptor subtypes ( SSTR 1 SSTR5 ) were coexpressed with a subunit of G protein gated inwardly rectifying potassium channel ( GIRK 1 ) in Xenopus oocytes . ^^^ SSTR 2 SSTR5 , but not SSTR 1 coupled efficiently to the activation of GIRK currents when stimulated by SST 14 or SST 28 . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| We have analysed the expression of somatostatin receptor subtype ( SSTR 1 5 ) mRNA in normal and malignant breast tissue . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Distribution of somatostatin receptors ( SSTR 1 5 ) was determined in the rat eye . ^^^ The differential expression of SSTR 1 5 may be related to distinct roles of somatostatin in the physiology of different ocular tissues . . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Somatostatin alone , GMPPNP alone , or somatostatin plus GDP were ineffective under these conditions . sstr 1 and sstr 5 failed to increase PTPase activity although both receptors were expressed , as assessed by appearance of high affinity binding sites for [ 125I Tyr 11 ] somatostatin 14 . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| This region , 14q12 q 13 , may contain changes in one of the five known somatostatin receptor genes ( SSTR 1 ) . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Somatostatin activation of mitogen activated protein kinase via somatostatin receptor 1 ( SSTR 1 ) . ^^^ Here we examine the actions of the hormone somatostatin on the MAP kinase cascade through one of its two major receptor subtypes , the somatostatin receptor 1 ( SSTR 1 ) stably expressed in CHO K 1 cells . ^^^ Somatostatin antagonizes the proliferative effects of fibroblast growth factor in CHO SSTR 1 cells via the SSTR 1 receptor . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| These analogs bind with high affinity to only two SST receptor ( SSTR ) subtypes ( 2 and 5 ) , display moderate affinity for SSTR 3 , and low affinity for SSTR 1 and 4 . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Structural basis for the binding specificity of a SSTR 1 selective analog of somatostatin . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Identification of distinct signalling pathways for somatostatin receptors SSTR 1 and SSTR 2 as revealed by microphysiometry . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Five distinct membrane receptors ( SSTR 1 5 ) for SST are known , and at least two ( SSTR 2 and SSTR 5 ) have been proposed to regulate pancreatic endocrine function . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Primary cell cultures from pituitaries of wild type SSTR 1 mice exposed to the SSTR 1 selective somatostatin analog CH 275 show reduction of basal levels of GH secretion whereas somatotrophs isolated from SSTR 1 null mutant mice did not respond to the agonist mediated effect . ^^^ The various functions of SST are mediated through at least five different receptor subtypes ( SSTR 1 5 ) , their precise physiological roles have not been solved yet . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| These assumptions were tested in 20 human cell lines derived from malignant gliomas and 4 glioblastoma tissue specimens , which were analyzed for their expression of the five known somatostatin receptor genes ( SSTR 1 5 ) and for the receptor function . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Localization of somatostatin receptor subtype mRNA in the rat gastrointestinal tract and regulation of SSTR 1 gene expression . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| We hypothesize that SST 14 acts on PTK activity in colonic cancer mainly via SSTR 1 subtype of somatostatin receptors . . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Exposure of these cells to 10 nM somatostatin or 10 nM SSTR 1 specific agonist resulted in alterations to the actin cytoskeleton , as characterized by a loss of actin stress fibers coupled with an increase in lamellipodia formation at the plasma membrane . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| The expression of messenger ribonucleic acid ( mRNA ) for SST receptor subtype 1 ( SSTR 1 ) , subtype 2 ( SSTR 2 ) , and subtype 5 ( SSTR 5 ) was also investigated in 22 samples by RT PCR . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| In the present study , we have assessed the role of a series of SST analogs , three of which were selective for SSTR 1 , SSTR 2 or SSTR 5 , in these cellular events . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Somatostatin receptor 1 ( SSTR 1 ) mediated inhibition of cell proliferation correlates with the activation of the MAP kinase cascade : role of the phosphotyrosine phosphatase SHP 2 . ^^^ In this review we examine recent data concerning the effects of somatostatin on the MAP kinase cascade through one of its major receptor subtype , the somatostatin receptor 1 ( SSTR 1 ) , stably expressed in CHO K 1 cells . ^^^ Somatostatin inhibits the proliferative effects of basic FGF ( bFGF ) in CHO SSTR 1 cell line . ^^^ Previous studies have suggested that somatostatin stimulated PTP activity mediates the growth inhibitory actions of somatostatin , in CHO SSTR 1 cells . ^^^ Thus , the activation of SHP 2 by SSTR 1 may mediate the antiproliferative activity of somatostatin . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| The cytochrome P 450 , subfamily 1 , polypeptide 1 ( CYP1A1 ) , somatostatin receptor 1 ( SSTR 1 ) and transcription termination factor 1 ( TTF 1 ) genes , predicted to reside on SSC7q based on chromosome painting studies , were selected for genetic mapping in the pig . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Association analysis of somatostatin receptor ( SSTR 1 and SSTR 2 ) polymorphisms in breast cancer and solar keratosis . ^^^ The presence of somatostatin receptors ( SSTR 1 5 ) in tumour cells indicates a potential for somatostatin to bind and suppress growth , as well as allowing for therapeutic treatment with somatostatin analogues . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Somatostatin and its receptors ( SSTR 1 to SSTR 5 ) are expressed in normal human parafollicular C cells and medullary thyroid carcinoma ( MTC ) , but the role of SSTR subtypes in cell growth regulation is still not clear . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| We have examined normal T cells and T cell lines with respect to expression of various somatostatin receptor subtypes ( SSTR 1 5 ) using RT PCR and PCR . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| The effects of somatostatin ( SRIF ) are mediated through five distinct G protein coupled receptors ( SSTR 1 5 ) . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND AND AIMS : Recently , novel somatostatin receptor ( sstr ) subtype specific ligand analogues have been developed for medical treatment of neuroendocrine tumours expressing different sstrs ( sstr 1 5 ) . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| To show that native SSTR 1 shares the ability to inhibit Rho activation , we demonstrated that somatostatin treatment of human umbilical vein endothelial cells attenuated thrombin stimulated Rho GTP accumulation . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Somatostatin ( SRIH ) analogs are commonly used to treat symptoms in medullary thyroid carcinoma ( MTC ) , that expresses SRIH receptors ( SSTR 1 to SSTR 5 ) , as does the human MTC cell line TT . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| INTRODUCTION : Somatostatin is an inhibitory peptide that exerts its effects tissue specifically by activating one or more of five receptors ( SSTR 1 5 ) . ^^^ AIM : To examine the effects of somatostatin and newly synthesized selective SSTR agonists on the cholinergically stimulated expression of the immediate early response gene METHODOLOGY AND RESULTS : In pancreatic acinar AR42J cells , polymerase chain reaction analysis revealed that mRNAs for SSTR 1 , 2 , and 3 were expressed . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Although somatostatin can bind to five different G protein coupled receptors ( SSTRs ) , only SSTR 1 and SSTR2A proteins have been described convincingly in rat and / or human kidneys . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Localization of messenger ribonucleic acids for somatostatin receptor subtypes ( sstr 1 5 ) in the rat adrenal gland . ^^^ Somatostatin ( somatotropin release inhibitory factor , SRIF ) exerts multiple inhibitory actions throughout the central nervous system and the periphery by binding to specific membrane bound SRIF receptors ( sstrs ) of which five subtypes ( sstr 1 5 ) have now been identified . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| A T ( s , p ) 5 motif within intracellular domain 2 and a QQ ( r ) motif within intracellular domain 3 are shared by the somatostatin receptor subtypes SSTR 1 , 3 , and 4 , which couple to the inhibition of NHE 1 , but not by SSTR 2 and 5 , which do not signal to NHE 1 . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| A new SSTR2+5 bispecific analog with high affinity and selectivity for both SSTR 2 and SSTR 5 , and a somatostatin analog with a unique broad receptor ( SSTR 1 , 2 , 3 , and 5 ) binding profile , are both able to inhibit in vitro GH release in GH cell adenomas partially sensitive to octreotide . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Somatostatin receptors were identified in 84 % of the tumors analyzed by RT PCR analysis . sstr 1 and sstr 2 were the receptors most frequently detected . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| The adrenal medulla tissue was examined for somatostatin ( SRIH ) receptor subtypes 1 to 5 ( SSTR 1 to 5 ) expression by RT PCR . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| The activation of SHP 1 , which is attributed to the SSTR 2 receptor and negatively regulates the EGF receptor , corresponds with the ability of cCD 2 to inhibit the EGF induced MAPK activation in COS 7 cells . ( 7 ) Our results show that in SH SY5Y cells cCD 2 inhibits cell growth via the SSTR 1 receptor signalling pathway but may , in other cells , also use other SSTR subtypes and their signalling mechanisms . ( 8 ) cCD 2 represents a novel type of opioid derived antiproliferative SST receptor agonist , which possesses low mu receptor affinity but may induce mu receptor sensitization and is structurally different from the hitherto known SST receptor agonists . . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| The actions of somatostatin ( SST ) in the nervous system are mediated by specific high affinity SST receptors ( SSTR 1 5 ) . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Among the five different G protein coupled somatostatin receptors ( SSTRs ) , SSTR 1 is expressed in human kidney collecting ducts and SSTR 2 is expressed in rat and human collecting ducts and glomeruli . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| SOM 230 , similar to somatostatin , has high affinity binding to SSTR 1 , 2 , 3 , and 5 and , in keeping with this , has an equivalent inhibitory effect on pituitary hormone secretion . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Here , we analyzed the effects of SST on nitric oxide ( NO ) production induced by different agonists [ basic fibroblast growth factor ( bFGF ) , insulin , cholecystokinin ( CCK ) ] and the intracellular signaling involved , using Chinese hamster ovary k 1 cells stably transfected with individual SSTR 1 SSTR4 . bFGF and insulin induced endothelial nitric oxide synthase activity via the generation of ceramide or the Akt dependent phosphorylation of endothelial nitric oxide synthase , respectively . ^^^ SST inhibited NO production stimulated by bFGF through SST receptor 1 ( SSTR 1 ) , SSTR 2 , and SSTR 3 and by CCK through SSTR 2 and SSTR 3 . ^^^ |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Interestingly , somatostatin receptor ( sstr 1 ) expression was increased by SREBP1c , whereas AMPK induced the expression of peptide YY , the early endocrine pancreas marker . . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| We characterized gastric acid secretion in SSTR 2 knockout mice , and used preferential somatostatin receptor agonists to assess the relative role of SSTR 1 , 2 , 3 , 4 , and 5 on gastric acid secretion . ^^^ |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| The mRNA levels of SST , SSTR 1 5 , as well as a housekeeping gene ( beta actin ) were determined by real time RT PCR . ^^^ RESULTS : Without TNF alpha treatment , HCAECs showed mRNA expression of SST , SSTR 1 , SSTR 2 , and SSTR 5 . ^^^ After treatment with TNF alpha , the mRNA levels of SST , SSTR 1 , SSTR 2 , and SSTR 5 were significantly reduced in a dose dependent fashion . ^^^ TNF alpha ( 1 ng / ml ) reduced SST , SSTR 1 , SSTR 2 , and SSTR 5 by 93 , 51 , 85 , and 99 % , respectively , compared to controls ( P < 0 . 001 , t test ) . ^^^ CONCLUSIONS : HCAECs express SST , SSTR 1 , SSTR 2 , and SSTR 5 , which are all decreased by TNF alpha treatment . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Pancreata were removed and levels of insulin , glucagon , somatostatin , and SSTR 1 were studied using immunohistochemical analysis along with H & E staining of the pancreata . ^^^ SSTR 1 expression was significantly increased in islets at 3 months of age , but was nearly absent in islets at 12 months of age , as was somatostatin staining in SSTR 5 / mice . ^^^ |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Transfection of SSTR 1 and SSTR 2 inhibits Panc 1 cell proliferation and renders Panc 1 cells responsive to somatostatin analogue . ^^^ Cell growth rate in Panc 1 cells transfected with SSTR 1 and SSTR 2 was inhibited about 41 % , and the cell proliferation of Panc 1 expressing SSTR 1 and SSTR 2 was further reduced about 12 % on treatment with somatostatin analogue as compared with the control group . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| This action has been considered to be generally mediated via six different specific somatostatin receptors ( sstr ) , sstr 1 , sstr2A , sstr2B , sstr 3 , sstr 4 , and sstr 5 . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| The structure of 1 is compared with the consensus structural motifs of other somatostatin analogues that bind predominantly to SSTR 1 , SSTR2 / SSTR5 and SSTR 4 receptors , and to the 3D structure of a non selective SRIF analogue , cyclo ( 3 14 ) H Cys ( 3 ) Phe ( 6 ) Tyr ( 7 ) D 2Nal ( 8 ) Lys ( 9 ) Thr ( 10 ) Phe ( 11 ) Cys ( 14 ) OH ( des AA ( 1 , 2 , 4 , 5 , 12 , 13 ) [ Tyr ( 7 ) , D 2Nal ( 8 ) ] SRIF ) ( peptide 2 ) . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Immunochemical analysis showed that the pancreatic islets of SSTR 1 / mice had significantly decreased levels of somatostatin staining and a significant decrease of SSTR 5 expression . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| SOM 230 is a novel somatostatin analog which shows affinity to 4 of the 5 known somatostatin receptors ( SSTR 1 5 ) . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| The development of specific somatostatin subtypes analogs , mainly for SSTR 5 , of a SSTR 2 SSTR5 bispecific compound , and of a `` universal ' ' analog with high affinity to SSTR 1 , 2 , 3 , and 5 showed preliminary , albeit promising results for the treatment of resistant somatotropic adenomas . . ^^^ |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| Expression of somatostatin receptor subtypes ( SSTR 1 5 ) in Alzheimer ' s disease brain : an immunohistochemical analysis . ^^^ Somatostatin , widely distributed in human cortical brain regions , acts through specific high affinity somatostatin receptors ( SSTR 1 5 ) to exert profound effects on motor , sensory , behavioral , cognitive and autonomic functions . ^^^ In the present study , the distributional pattern of SSTR 1 5 antigens in the frontal cortex of AD and age matched control brains was studied using antipeptide polyclonal rabbit antibodies directed against the five human somatostatin receptor subtypes . ^^^ |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30872 and P61278 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| We previously reported the cloning of two distinct somatostatin receptor ( SSTR ) subtypes , SSTR 1 and SSTR 2 . ^^^ Although both SSTR 1 and SSTR 2 bound somatostatin specifically and with high affinity , neither was coupled to adenylyl cyclase , a major cellular effector of somatostatin ' s actions . ^^^ |
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| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Stably transfected Chinese hamster ovary cells expressing SSTR 1 or SSTR 2 exhibit specific somatostatin binding , with an apparently higher affinity for somatostatin 14 than somatostatin 28 , and NH 2 terminally extended form of somatostatin 14 . ^^^ A SSTR 1 probe hybridized to multiple DNA fragments in EcoRI digests of human and mouse DNA , indicating that SSTR 1 and SSTR 2 are members of a larger family of somatostatin receptors . ^^^ |
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| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Previous studies have shown that at least two subtypes of somatostatin ( SRIF ) receptors ( SRIF 1 and SRIF 2 ) are expressed in mammalian cells . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| The amino acid sequence of rSSTR 4 is 60 % and 48 % identical to that of somatostatin receptors SSTR 1 and SSTR 2 , respectively , two recently cloned subtypes . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| The pharmacological properties of mSSTR 3 and its ability to couple with adenylylcyclase distinguish SSTR 3 from the other cloned somatostatin receptors and indicates that it mediates biological functions different from SSTR 1 or SSTR 2 . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| The genes encoding two different somatostatin receptors ( SSTRs ) have been isolated and characterized , and RNA blotting studies have shown that both SSTR 1 and SSTR 2 are expressed in the brain . ^^^ In order to gain a better understanding of the functions of somatostatin in the CNS , the distribution of SSTR 1 and SSTR 2 mRNAs was determined using the technique of in situ hybridization . ^^^ Several regions of the brain reported to contain dense somatostatin like immunoreactive terminal fields and receptor binding sites were devoid of both SSTR 1 and SSTR 2 mRNA , suggesting the existence of additional SSTR subtypes . . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Multiple effector coupling of somatostatin receptor subtype SSTR 1 . ^^^ The signal transduction pathways of a cloned human somatostatin receptor subtype , SSTR 1 , have been investigated in CHO cells stably expressing this receptor . ^^^ In SSTR 1 expressing CHO cells , somatostatin 14 inhibits forskolin stimulated cAMP formation in a dose dependent manner with an ED 50 of 1 . 0 10 10 ( 9 ) M . ^^^ Somatostatin 14 inhibitory action on adenylyl cyclase and stimulatory action on inositol 1 , 4 , 5 trisphosphate formation are both blocked by pertussis toxin , indicating that these effects of SSTR 1 are mediated by pertussis toxin sensitive G protein ( s ) . ^^^ Antiserum against Gi alpha 3 blocked the inhibitory effects of somatostatin 14 on forskolin stimulated adenylyl cyclase , but antiserum against Gi alpha 1 / Gi alpha 2 did not , indicating that Gi alpha 3 dominantly couples SSTR 1 to adenylyl cyclase . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| In rat GH 3 pituitary tumour cells exposed to 1 microM somatostatin for up to 48 h , sstr 1 , 3 , 4 and 5 mRNA increased dramatically while sstr 2 mRNA exhibited a biphasic response . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| With the aim of elucidating the role of individual somatostatin receptors in the central control of growth hormone secretion , we have examined the distribution of sstr 1 and sstr 2 mRNAs in the hypothalamus of the adult rat by in situ hybridization using 35S labelled antisense riboprobes . ^^^ Within the arcuate nucleus , the distribution of cells expressing sstr 1 and sstr 2 was comparable to that of neurons which bind somatostatin 14 selectively , one third of which have been documented to contain growth hormone releasing hormone . ^^^ Within the periventricular nucleus , the distribution of cells expressing sstr 1 and , to a lesser extent , sstr 2 was reminiscent of that of both [ 125I ] somatostatin labelled and somatostatin immunoreactive cells . ^^^ Taken together , these results imply a role for both sstr 1 and sstr 2 receptors in the central regulation of growth hormone releasing hormone and somatostatin secretion , and hence of growth hormone release , by somatostatin . . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| It appears from this study that the rat lateral cerebellar nuclei contain a pure population of receptors exhibiting the same binding characteristics as the recently cloned sstr 1 somatostatin receptor . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Patterns of expression of SSTR 1 and SSTR 2 somatostatin receptor subtypes in the hypothalamus of the adult rat : relationship to neuroendocrine function . ^^^ Taken together , these distributional patterns conform to those of somatostatin binding sites as visualized by in vitro autoradiography , suggesting that an important proportion of SSTR 1 and SSTR 2 receptors in the hypothalamus are associated with the perikarya and dendrites of intrinsic neurons . ^^^ The distribution of SSTR 1 expressing cells within the periventricular , paraventricular and suprachiasmatic nuclei was similar to that of neurons previously reported to contain and / or express somatostatin in the brain suggesting that some of the SSTR 1 receptors may correspond to autoreceptors . ^^^ Within the arcuate nucleus , the distribution of SSTR 1 and SSTR 2 messenger RNA expressing cells was comparable to that of neurons previously found to selectively bind somatostatin 14 within this area . ^^^ Taken together , the present results suggest that SSTR 1 and SSTR 2 somatostatin receptor messenger RNAs are heavily expressed in those neurons containing somatostatin and / or growth hormone releasing factor and thereby imply a role for both SSTR 1 and SSTR 2 somatostatin receptor subtypes in neuroendocrine regulation of growth hormone secretion in both sexes of this species . . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Pharmacological identity between somatostatin SS 2 binding sites and SSTR 1 receptors . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| The somatostatin receptor SSTR 1 is coupled to phosphotyrosine phosphatase activity in CHO K 1 cells . ^^^ To determine whether cloned somatostatin receptors could mediate coupling to phosphotyrosine phosphotyrosine phosphatase activity , we examined phosphatase activity after somatotostatin activation of the rat somatostatin receptors SSTR 1 and SSTR 2 after their stable expression in heterologous Chinese Hamster Ovary ( CHO K 1 ) cells . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| In radioligand binding studies we have compared the binding properties of sstr 1 5 with their activities as somatostatin receptors . ^^^ The similarities in receptor sequence and in the binding profiles of short synthetic somatostastin analogues such as octreotide , MK 678 or RC 160 for sstr 1 5 indicate the existence of two classes of receptors sstr1 / sstr4 with virtually no or very low affinity and sstr2 / sstr3 / sstr5 with intermediate to high affinity for the short somatostatin analogues . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| In 1992 , a major breakthrough in our understanding of somatostatin functions was made with the cloning of five different receptor genes ( sstr 1 to sstr 5 ) which belong to the seven transmembrane domain receptor family . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Finally , we studied the expression of the somatostatin receptor gene by both ribonuclease ( RNase ) protection and nuclear run on assays using cDNA encoding for two subtypes of the somatostatin receptor gene ( i . e . , SSTR 1 and SSTR 2 ) . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| A large number of somatostatin analogs taken from several major families of peptides has been examined for binding to three newly discovered somatostatin receptors ( SSTR 1 , 2 and 3 ) transfected and expressed in various cell membrane preparations . ^^^ Whereas all of the octapeptides had much lower affinity for SSTR 1 and SSTR 3 receptors , which appear to be primarily present in the CNS , high affinity and highly specific ligands for the latter were found within a series of linear somatostatin analogs . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Gene expression of somatostatin receptor subtypes , SSTR 1 and SSTR 2 , in human lung cancer cell lines . ^^^ In the present study , we investigated the expression of somatostatin receptor ( SSTR ) subtype ( SSTR 1 and SSTR 2 ) mRNA in various human lung cancer cell lines by the sensitive reverse transcription PCR method and Southern blotting . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Effects of the stable somatostatin analogue RC 160 on cell proliferation , tyrosine phosphatase activity , and intracellular calcium concentration were investigated in CHO cells expressing the five somatostatin receptor subtypes SSTR 1 to 5 . ^^^ Binding experiments were performed on crude membranes by using [ 125I labeled Tyr 11 ] somatostatin 14 ; RC 160 exhibited moderate to high affinities for SSTR 2 , 3 , and 5 ( IC 50 , 0 . 17 , 0 . 1 and 21 nM , respectively ) and low affinity for SSTR 1 and 4 ( IC 50 , 200 and 620 nM , respectively ) . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Human somatostatin receptor subtypes ( SSTR 1 5 ) bind their natural ligands SRIF 14 and SRIF 28 with high affinity . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Two classes of somatostatin receptors , SRIF 1 and SRIF 2 , have been distinguished biochemically and pharmacologically . ^^^ The pharmacological characteristics of receptors expressing these cDNAs resemble those of the SRIF 2 and SRIF 1 classes of somatostatin receptors , respectively . ^^^ The somatostatin receptors SSTR 1 and SSTR 2 are coupled to inhibition of adenylyl cyclase in Chinese hamster ovary cells via pertussis toxin sensitive pathways . ^^^ Two cDNAs have been recently isolated that encode somatostatin receptors 1 and 2 ( SSTR 1 and SSTR 2 , respectively ) . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Stimulation of tyrosine phosphatase and inhibition of cell proliferation by somatostatin analogues : mediation by human somatostatin receptor subtypes SSTR 1 and SSTR 2 . ^^^ The effects of somatostatin analogues RC 160 and SMS 201 995 on tyrosine phosphatase and cell proliferation were investigated in COS 7 and NIH 3T3 cells expressing human somatostatin receptor subtype 1 or 2 ( SSTR 1 or SSTR 2 ) . ^^^ The somatostatin analogues RC 160 and SMS 201 995 exhibited low affinity for SSTR 1 ( IC 50 of 0 . 43 and 1 . 5 microM , respectively ) and high affinity for SSTR 2 ( IC 50 of 0 . 27 and 0 . 19 nM ) . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| COS 7 cells were transfected with human somatostatin ( SRIF ) receptor type 1 and 2 ( human SSTR 1 and SSTR 2 , respectively ) cDNAs . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Expression and localization of somatostatin receptor SSTR 1 , SSTR 2 , and SSTR 3 messenger RNAs in primary human tumors using in situ hybridization . ^^^ Somatostatin receptor gene expression of SSTR 1 , SSTR 2 , and SSTR 3 subtypes was evaluated by in situ hybridization in 55 human primary tumors shown to contain a high density of somatostatin receptors in binding assays . ^^^ The cases having SSTR 1 mRNA were identified in binding experiments with 125I labeled somatostatin 14 and 28 analogues rather than with 125I [ Tyr 3 ] octreotide . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| In the present study , we have investigated the affinities of a battery of SRIF analogues to bind to SRIF receptor subtypes SSTR 1 ( cloned somatostatin complex ) , SSTR 2 , and SSTR 3 , as well as their abilities to inhibit the release of growth hormone from anterior pituitary cells in vitro . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| To investigate Na+ regulation of agonist binding to somatostatin receptors , Na+ was tested for its effect on the binding of agonists to cloned somatostatin receptor type 1 ( SSTR 1 ) and somatostatin receptor type 2 ( SSTR 2 ) stably expressed in Chinese hamster ovary cells . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| The expression of two somatostatin receptor subtypes , SSTR 1 and SSTR 2 , was studied in 27 pituitary tumors and 1 chronic lymphocytic leukemia pituitary infiltrate . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| We recently have cloned five human somatostatin receptor subtypes ( SSTR 1 SSTR5 ) . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Subtype specific signaling mechanisms of somatostatin receptors SSTR 1 and SSTR 2 . ^^^ We expressed two somatostatin receptor subtypes , SSTR 1 and SSTR 2 , stably in mouse fibroblast Ltk cells and transiently in human embryonic kidney HEK 293 cells to investigate subtype specific pharmacological and functional properties . ^^^ The effects of GTP gamma S and pertussis toxin on [ 125I Tyr 11 ] somatostatin 14 binding indicated that SSTR 2 may couple exclusively to pertussis toxin sensitive G proteins , whereas SSTR 1 may couple to both pertussis sensitive and insensitive G proteins . ^^^ In contrast , only SSTR 1 mediated somatostatin inhibition of Na ( + ) H+ exchange activity , and this action was insensitive to pertussis toxin . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| We found that somatostatin inhibited cAMP accumulation in human embryonic kidney 293 cells only when cells were transfected with either SSTR 1 or SSTR 2 . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Rat somatostatin receptor type 1 couples to G proteins and inhibition of cyclic AMP accumulation . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Localization of somatostatin ( SRIF ) SSTR 1 , SSTR 2 and SSTR 3 receptor mRNA in rat brain by in situ hybridization . ^^^ In situ hybridization histochemistry was performed to analyse the distribution of the messenger RNA ( mRNA ) of three putative somatostatin ( SRIF ) receptors in rat brain , using oligonucleotide probes derived from the cDNA coding for SSTR 1 , SSTR 2 , and SSTR 3 receptors . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Differential expression of messenger RNAs for somatostatin receptor subtypes SSTR 1 , SSTR 2 and SSTR 3 in adult rat brain : analysis by RNA blotting and in situ hybridization histochemistry . ^^^ The messenger RNAs encoding three somatostatin receptor subtypes , SSTR 1 , SSTR 2 and SSTR 3 , were detected in rat by RNA blotting and in situ hybridization histochemistry to identify the sites of synthesis and expression of these somatostatin receptor subtypes . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| A review of prior data on other receptors suggests that this receptor may belong to a unique class of seven transmembrane receptors ( including somatostatin SSTR 1 , dopamine D 3 , and frizzled protein Fz ) for which G protein coupling has not been demonstrated . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| The tissue distribution of mRNA encoding five somatostatin receptor subtypes , SSTR 1 , SSTR 2 , SSTR 3 , SSTR 4 , and SSTR 5 , was determined in adult rat tissues by solution hybridization / nuclease protection analysis using sequence specific cRNA probes . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Among members of the G protein coupled receptor superfamily , the sequences of both msl 1 and msl 1 were most closely related to those of the somatostatin receptors ( SSTRs ) , having approximately 35 % identity with the sequence of SSTR 1 . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| The mRNA distribution in the brain and the coupling to cellular effector systems of four somatostatin receptors ( SSTR 1 4 ) was studied . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| The genes encoding three different human somatostatin receptor subtypes , designated SSTR 1 , SSTR 2 , and SSTR 3 , were mapped to chromosomes 14 , 17 , and 22 , respectively , by analyzing their segregation in a panel of reduced human hamster somatic cell hybrids . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Five seven transmembrane domain somatostatin receptor subtypes ( SSTR 1 5 ) have been recently cloned and only SSTR 1 has been shown to be present in the human stomach . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| To increase further the reliability and prognostic value of sstr identification , we investigated the presence of mRNA for the subtypes sstr 1 and sstr 2 by in situ hybridization on tumor specimen from 25 carcinoid patients ( 22 midgut , 2 foregut , and 1 hindgut ) , all receiving somatostatin analogue treatment ( 12 lanreotide , 8 octreotide , and 5 octastatin ) and compared this to the therapeutic response evaluated as inhibition of hormone secretion . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| The effects of octreotide , a long acting somatostatin agonist selective of the sstr2 / sstr3 / sstr5 receptor subtypes , on ectopic GH secretion and tumor growth were investigated in Wistar Furth female rats implanted with GH secreting ( GC ) cells which express mostly somatostatin receptors of the sstr 1 and sstr 2 subtypes . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| In order to investigate the gonadal regulation of pituitary somatostatin receptors ( SSTRs ) gene expression , we studied the existence of sexual dimorphism on SSTR 1 , SSTR 2 , and SSTR 3 mRNA levels in the rat anterior pituitary . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Development of a selective agonist at the somatostatin receptor subtype sstr 1 . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| The messenger RNA ( mRNA ) expression of somatostatin ( SRIF ) receptors SSTR 1 , SSTR 2 , SSTR 3 , SSTR 4 and SSTR 5 ( called sst 1 5 , now ) was studied in rat brain between embryonic day 17 ( E 17 ) and post natal day 5 ( P 5 ) by in situ hybridization histochemistry and compared to that of adult rats . sst 1 receptor mRNA expression was very low and restricted at E 17 , spread out at E 18 , to reach very high levels comparable to that of adult at P 5 ( e . g . in temia tecta , posteromedial cortical amygdaloid nucleus , subiculum ) . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Pharmacological characterisation of human cerebral cortex somatostatin SRIF 1 and SRIF 2 receptors . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Autoradiographic analysis of somatostatin SRIF 1 and SRIF 2 receptors in the human brain and pituitary . ^^^ The distribution of somatostatin ( SRIF ) receptor sites was studied by in vitro receptor autoradiography in the human brain and pituitary using the SRIF 1 ( sst 2 ) receptor selective [ 125I ] Tyr 3 octreotide , the non subtype selective [ 125I ] LTT SRIF 28 ( [ Leu 8 , D Trp 22 , 125I Tyr 25 ] SRIF 28 ) and the SRIF 2 receptor selective [ 125I ] CGP 23996 ( c [ Asu Lys Asn Phe Phe Trp Lys Thr Tyr Thr Ser ] ) in buffer containing 120 mM Na+ . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| We have analysed the expression of somatostatin receptor subtype ( SSTR 1 5 ) mRNA in normal and malignant breast tissue . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Distribution of somatostatin receptors ( SSTR 1 5 ) was determined in the rat eye . ^^^ The differential expression of SSTR 1 5 may be related to distinct roles of somatostatin in the physiology of different ocular tissues . . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Somatostatin ( SRIF ) receptor subtypes ( sst ) were characterized in hypothalamic neurons and astrocytes by quantitative reverse transcription polymerase chain reaction and radioreceptor assays using [ 125I Tyr 0 , D Trp 8 ] SRIF 14 as a ligand in ionic conditions discriminating between SRIF 1 ( sst 2 , 3 , and 5 receptors ) and SRIF 2 ( sst 1 and 4 receptors ) binding sites . ^^^ |
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| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Somatostatin alone , GMPPNP alone , or somatostatin plus GDP were ineffective under these conditions . sstr 1 and sstr 5 failed to increase PTPase activity although both receptors were expressed , as assessed by appearance of high affinity binding sites for [ 125I Tyr 11 ] somatostatin 14 . ^^^ |
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| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| This region , 14q12 q 13 , may contain changes in one of the five known somatostatin receptor genes ( SSTR 1 ) . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Both overlapping and distinct signaling pathways for somatostatin receptor subtypes SSTR 1 and SSTR 2 in pituitary cells . ^^^ To elucidate the signaling events mediated by specific somatostatin receptor ( SSTR ) subtypes , we expressed SSTR 1 and SSTR 2 individually in rat pituitary GH12C1 and F4C1 cells , which lack endogenous somatostatin receptors . ^^^ In transfected F4C1 cells , both SSTR 1 and SSTR 2 mediated somatostatin induced inhibition of adenylyl cyclase via a PTx sensitive pathway . ^^^ Thus , by expressing individual somatostatin receptor subtypes in pituitary cells , we have identified both overlapping and distinct signaling pathways for SSTR 1 and SSTR 2 , and have shown that sequences other than simply the intracellular domains are required for SSTR 2 to couple to the PLC signaling pathway . . ^^^ |
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| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| To illustrate the broad applicability of this technique , we have presented data from cell lines expressing a glycoprotein hormone receptor for follicle stimulating hormone ( FSHR ) , CXC ( CXCR 2 ) , and CC chemokine ( CCR 1 ) receptors and peptide receptors from the somatostatin ( SSTR 1 , 2 , 5 ) and neuropeptide Y ( NPY Y 2 , Y 4 Rs ) families . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Recent molecular studies have revealed the existence of up to five membrane somatostatin receptor subtypes termed SSTR 1 5 . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Expression of the five somatostatin receptor ( SSTR 1 5 ) subtypes in rat pituitary somatotrophes : quantitative analysis by double layer immunofluorescence confocal microscopy . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| We analyzed the mRNA expression of somatostatin receptor subtypes 1 to 5 ( SSTR 1 5 ) in human lymphoid cell lines , human peripheral blood lymphocytes ( PBL ) , and human lymphatic leukemia cells , using the reverse transcription polymerase chain reaction method . ^^^ |
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| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| The five different rat somatostatin receptor subtypes ( SSTR 1 SSTR5 ) were coexpressed with a subunit of G protein gated inwardly rectifying potassium channel ( GIRK 1 ) in Xenopus oocytes . ^^^ |
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| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| These assumptions were tested in 20 human cell lines derived from malignant gliomas and 4 glioblastoma tissue specimens , which were analyzed for their expression of the five known somatostatin receptor genes ( SSTR 1 5 ) and for the receptor function . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Localization of somatostatin receptor subtype mRNA in the rat gastrointestinal tract and regulation of SSTR 1 gene expression . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| We hypothesize that SST 14 acts on PTK activity in colonic cancer mainly via SSTR 1 subtype of somatostatin receptors . . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Somatostatin activation of mitogen activated protein kinase via somatostatin receptor 1 ( SSTR 1 ) . ^^^ Here we examine the actions of the hormone somatostatin on the MAP kinase cascade through one of its two major receptor subtypes , the somatostatin receptor 1 ( SSTR 1 ) stably expressed in CHO K 1 cells . ^^^ Somatostatin antagonizes the proliferative effects of fibroblast growth factor in CHO SSTR 1 cells via the SSTR 1 receptor . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Structural basis for the binding specificity of a SSTR 1 selective analog of somatostatin . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Identification of distinct signalling pathways for somatostatin receptors SSTR 1 and SSTR 2 as revealed by microphysiometry . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Agonist dependent up regulation of human somatostatin receptor type 1 requires molecular signals in the cytoplasmic C tail . ^^^ We have previously reported that the human somatostatin receptor type 1 ( hSSTR 1 ) stably expressed in Chinese hamster ovary K 1 cells does not internalize but instead up regulates at the membrane during continued agonist treatment ( 1 microM somatostatin ( SST ) 14 10 22 h ) . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Primary cell cultures from pituitaries of wild type SSTR 1 mice exposed to the SSTR 1 selective somatostatin analog CH 275 show reduction of basal levels of GH secretion whereas somatotrophs isolated from SSTR 1 null mutant mice did not respond to the agonist mediated effect . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Exposure of these cells to 10 nM somatostatin or 10 nM SSTR 1 specific agonist resulted in alterations to the actin cytoskeleton , as characterized by a loss of actin stress fibers coupled with an increase in lamellipodia formation at the plasma membrane . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Somatostatin receptor 1 ( SSTR 1 ) mediated inhibition of cell proliferation correlates with the activation of the MAP kinase cascade : role of the phosphotyrosine phosphatase SHP 2 . ^^^ In this review we examine recent data concerning the effects of somatostatin on the MAP kinase cascade through one of its major receptor subtype , the somatostatin receptor 1 ( SSTR 1 ) , stably expressed in CHO K 1 cells . ^^^ Somatostatin inhibits the proliferative effects of basic FGF ( bFGF ) in CHO SSTR 1 cell line . ^^^ Previous studies have suggested that somatostatin stimulated PTP activity mediates the growth inhibitory actions of somatostatin , in CHO SSTR 1 cells . ^^^ Thus , the activation of SHP 2 by SSTR 1 may mediate the antiproliferative activity of somatostatin . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| The cytochrome P 450 , subfamily 1 , polypeptide 1 ( CYP1A1 ) , somatostatin receptor 1 ( SSTR 1 ) and transcription termination factor 1 ( TTF 1 ) genes , predicted to reside on SSC7q based on chromosome painting studies , were selected for genetic mapping in the pig . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Association analysis of somatostatin receptor ( SSTR 1 and SSTR 2 ) polymorphisms in breast cancer and solar keratosis . ^^^ The presence of somatostatin receptors ( SSTR 1 5 ) in tumour cells indicates a potential for somatostatin to bind and suppress growth , as well as allowing for therapeutic treatment with somatostatin analogues . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Somatostatin and its receptors ( SSTR 1 to SSTR 5 ) are expressed in normal human parafollicular C cells and medullary thyroid carcinoma ( MTC ) , but the role of SSTR subtypes in cell growth regulation is still not clear . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| We have examined normal T cells and T cell lines with respect to expression of various somatostatin receptor subtypes ( SSTR 1 5 ) using RT PCR and PCR . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| The effects of somatostatin ( SRIF ) are mediated through five distinct G protein coupled receptors ( SSTR 1 5 ) . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND AND AIMS : Recently , novel somatostatin receptor ( sstr ) subtype specific ligand analogues have been developed for medical treatment of neuroendocrine tumours expressing different sstrs ( sstr 1 5 ) . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| To show that native SSTR 1 shares the ability to inhibit Rho activation , we demonstrated that somatostatin treatment of human umbilical vein endothelial cells attenuated thrombin stimulated Rho GTP accumulation . ^^^ |
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| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Somatostatin ( SRIH ) analogs are commonly used to treat symptoms in medullary thyroid carcinoma ( MTC ) , that expresses SRIH receptors ( SSTR 1 to SSTR 5 ) , as does the human MTC cell line TT . ^^^ |
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| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| INTRODUCTION : Somatostatin is an inhibitory peptide that exerts its effects tissue specifically by activating one or more of five receptors ( SSTR 1 5 ) . ^^^ AIM : To examine the effects of somatostatin and newly synthesized selective SSTR agonists on the cholinergically stimulated expression of the immediate early response gene METHODOLOGY AND RESULTS : In pancreatic acinar AR42J cells , polymerase chain reaction analysis revealed that mRNAs for SSTR 1 , 2 , and 3 were expressed . ^^^ |
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| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Although somatostatin can bind to five different G protein coupled receptors ( SSTRs ) , only SSTR 1 and SSTR2A proteins have been described convincingly in rat and / or human kidneys . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Localization of messenger ribonucleic acids for somatostatin receptor subtypes ( sstr 1 5 ) in the rat adrenal gland . ^^^ Somatostatin ( somatotropin release inhibitory factor , SRIF ) exerts multiple inhibitory actions throughout the central nervous system and the periphery by binding to specific membrane bound SRIF receptors ( sstrs ) of which five subtypes ( sstr 1 5 ) have now been identified . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| A T ( s , p ) 5 motif within intracellular domain 2 and a QQ ( r ) motif within intracellular domain 3 are shared by the somatostatin receptor subtypes SSTR 1 , 3 , and 4 , which couple to the inhibition of NHE 1 , but not by SSTR 2 and 5 , which do not signal to NHE 1 . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| A new SSTR2+5 bispecific analog with high affinity and selectivity for both SSTR 2 and SSTR 5 , and a somatostatin analog with a unique broad receptor ( SSTR 1 , 2 , 3 , and 5 ) binding profile , are both able to inhibit in vitro GH release in GH cell adenomas partially sensitive to octreotide . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Somatostatin receptors were identified in 84 % of the tumors analyzed by RT PCR analysis . sstr 1 and sstr 2 were the receptors most frequently detected . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| The adrenal medulla tissue was examined for somatostatin ( SRIH ) receptor subtypes 1 to 5 ( SSTR 1 to 5 ) expression by RT PCR . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Somatostatin receptor type 1 , 2 , 3 , and 5 mRNA was detected only in tumoral tissue . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Somatostatin receptor type 1 was modelled based on the atomic structure of bovine rhodopsin . ^^^ Possible ways of binding interaction between somatostatin receptor type 1 and TT 232 , a cycloheptapeptide analogue of somatostatin with broad therapeutic potential , were analysed by molecular docking . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| The actions of somatostatin ( SST ) in the nervous system are mediated by specific high affinity SST receptors ( SSTR 1 5 ) . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Among the five different G protein coupled somatostatin receptors ( SSTRs ) , SSTR 1 is expressed in human kidney collecting ducts and SSTR 2 is expressed in rat and human collecting ducts and glomeruli . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| SOM 230 , similar to somatostatin , has high affinity binding to SSTR 1 , 2 , 3 , and 5 and , in keeping with this , has an equivalent inhibitory effect on pituitary hormone secretion . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Peptide chelating agent conjugate for selective targeting of somatostatin receptor type 1 : synthesis and characterization . ^^^ Previously reported results suggest that the analogue of the somatostatin des AA 1 , 2 , 5 [ D Trp 8 , IAmp 9 ] somatostatin ( CH 275 ) peptide bearing chelating agents able to coordinate radioactive metals could be used for scintigraphic imaging of tumor lesions overexpressing sstr 1 . ^^^ A preliminary biological assay of the binding ability of 111In DTPAGlu Gly CH 275 indicates , however , that the labeled compound does not display any specific interaction with somatostatin sstr 1 receptors in the tested cell lines . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Interestingly , somatostatin receptor ( sstr 1 ) expression was increased by SREBP1c , whereas AMPK induced the expression of peptide YY , the early endocrine pancreas marker . . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| We characterized gastric acid secretion in SSTR 2 knockout mice , and used preferential somatostatin receptor agonists to assess the relative role of SSTR 1 , 2 , 3 , 4 , and 5 on gastric acid secretion . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| The effects of somatostatin were insensitive to tetrodotoxin , reduced by Ba2+ , occluded or abolished by the presence of nonhydrolysable GTP or GDP analogues , respectively , and blocked or mimicked by a somatostatin receptor type 2 antagonist ( BIM 23627 ) or somatostatin receptor type 2 agonist ( L 779 , 976 ) , respectively , while somatostatin receptor type 1 , 3 and 4 agonists were ineffective ( L 797 , 591 , L 796 , 778 , L 803 , 087 ) . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Agonist induced up regulation of human somatostatin receptor type 1 is regulated by beta arrestin 1 and requires an essential serine residue in the receptor C tail . ^^^ We have previously shown that the human somatostatin receptor type 1 ( hSSTR 1 ) does not undergo agonist induced internalization , but is instead up regulated at the membrane upon prolonged somatostatin ( SST ) exposure . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Expression of somatostatin receptor subtypes ( SSTR 1 5 ) in Alzheimer ' s disease brain : an immunohistochemical analysis . ^^^ Somatostatin , widely distributed in human cortical brain regions , acts through specific high affinity somatostatin receptors ( SSTR 1 5 ) to exert profound effects on motor , sensory , behavioral , cognitive and autonomic functions . ^^^ In the present study , the distributional pattern of SSTR 1 5 antigens in the frontal cortex of AD and age matched control brains was studied using antipeptide polyclonal rabbit antibodies directed against the five human somatostatin receptor subtypes . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Pancreata were removed and levels of insulin , glucagon , somatostatin , and SSTR 1 were studied using immunohistochemical analysis along with H & E staining of the pancreata . ^^^ SSTR 1 expression was significantly increased in islets at 3 months of age , but was nearly absent in islets at 12 months of age , as was somatostatin staining in SSTR 5 / mice . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Transfection of SSTR 1 and SSTR 2 inhibits Panc 1 cell proliferation and renders Panc 1 cells responsive to somatostatin analogue . ^^^ Cell growth rate in Panc 1 cells transfected with SSTR 1 and SSTR 2 was inhibited about 41 % , and the cell proliferation of Panc 1 expressing SSTR 1 and SSTR 2 was further reduced about 12 % on treatment with somatostatin analogue as compared with the control group . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| This action has been considered to be generally mediated via six different specific somatostatin receptors ( sstr ) , sstr 1 , sstr2A , sstr2B , sstr 3 , sstr 4 , and sstr 5 . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| The structure of 1 is compared with the consensus structural motifs of other somatostatin analogues that bind predominantly to SSTR 1 , SSTR2 / SSTR5 and SSTR 4 receptors , and to the 3D structure of a non selective SRIF analogue , cyclo ( 3 14 ) H Cys ( 3 ) Phe ( 6 ) Tyr ( 7 ) D 2Nal ( 8 ) Lys ( 9 ) Thr ( 10 ) Phe ( 11 ) Cys ( 14 ) OH ( des AA ( 1 , 2 , 4 , 5 , 12 , 13 ) [ Tyr ( 7 ) , D 2Nal ( 8 ) ] SRIF ) ( peptide 2 ) . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Somatostatin receptor type 1 expression was significantly decreased , whereas type 4 receptor was expressed , which are unusual characteristics for pituitary tumors . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Immunochemical analysis showed that the pancreatic islets of SSTR 1 / mice had significantly decreased levels of somatostatin staining and a significant decrease of SSTR 5 expression . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| SOM 230 is a novel somatostatin analog which shows affinity to 4 of the 5 known somatostatin receptors ( SSTR 1 5 ) . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| The development of specific somatostatin subtypes analogs , mainly for SSTR 5 , of a SSTR 2 SSTR5 bispecific compound , and of a `` universal ' ' analog with high affinity to SSTR 1 , 2 , 3 , and 5 showed preliminary , albeit promising results for the treatment of resistant somatotropic adenomas . . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| This implies that SST analogs like SMS 201 995 , known to interact with SSTR2A , but not with SSTR 1 , act on pituitary tumors mainly via the SSTR 2 subtype . . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Five SST receptors have been characterized ( SSTR 1 to 5 ) . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| These analogs bind with high affinity to only two SST receptor ( SSTR ) subtypes ( 2 and 5 ) , display moderate affinity for SSTR 3 , and low affinity for SSTR 1 and 4 . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Five distinct membrane receptors ( SSTR 1 5 ) for SST are known , and at least two ( SSTR 2 and SSTR 5 ) have been proposed to regulate pancreatic endocrine function . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| The expression of messenger ribonucleic acid ( mRNA ) for SST receptor subtype 1 ( SSTR 1 ) , subtype 2 ( SSTR 2 ) , and subtype 5 ( SSTR 5 ) was also investigated in 22 samples by RT PCR . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| In the present study , we have assessed the role of a series of SST analogs , three of which were selective for SSTR 1 , SSTR 2 or SSTR 5 , in these cellular events . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| The activation of SHP 1 , which is attributed to the SSTR 2 receptor and negatively regulates the EGF receptor , corresponds with the ability of cCD 2 to inhibit the EGF induced MAPK activation in COS 7 cells . ( 7 ) Our results show that in SH SY5Y cells cCD 2 inhibits cell growth via the SSTR 1 receptor signalling pathway but may , in other cells , also use other SSTR subtypes and their signalling mechanisms . ( 8 ) cCD 2 represents a novel type of opioid derived antiproliferative SST receptor agonist , which possesses low mu receptor affinity but may induce mu receptor sensitization and is structurally different from the hitherto known SST receptor agonists . . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| Here , we analyzed the effects of SST on nitric oxide ( NO ) production induced by different agonists [ basic fibroblast growth factor ( bFGF ) , insulin , cholecystokinin ( CCK ) ] and the intracellular signaling involved , using Chinese hamster ovary k 1 cells stably transfected with individual SSTR 1 SSTR4 . bFGF and insulin induced endothelial nitric oxide synthase activity via the generation of ceramide or the Akt dependent phosphorylation of endothelial nitric oxide synthase , respectively . ^^^ SST inhibited NO production stimulated by bFGF through SST receptor 1 ( SSTR 1 ) , SSTR 2 , and SSTR 3 and by CCK through SSTR 2 and SSTR 3 . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| The mRNA levels of SST , SSTR 1 5 , as well as a housekeeping gene ( beta actin ) were determined by real time RT PCR . ^^^ RESULTS : Without TNF alpha treatment , HCAECs showed mRNA expression of SST , SSTR 1 , SSTR 2 , and SSTR 5 . ^^^ After treatment with TNF alpha , the mRNA levels of SST , SSTR 1 , SSTR 2 , and SSTR 5 were significantly reduced in a dose dependent fashion . ^^^ TNF alpha ( 1 ng / ml ) reduced SST , SSTR 1 , SSTR 2 , and SSTR 5 by 93 , 51 , 85 , and 99 % , respectively , compared to controls ( P < 0 . 001 , t test ) . ^^^ CONCLUSIONS : HCAECs express SST , SSTR 1 , SSTR 2 , and SSTR 5 , which are all decreased by TNF alpha treatment . ^^^ |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P61278 and P30872 |
Pubmed |
SVM Score :0.0 |
| NA |
|