| Interacting proteins: P60896 and P51587 |
Pubmed |
SVM Score :0.671794 |
| BRCA 2 associates with a 70 amino acid protein , DSS 1 , but the functional significance of this interaction has remained unclear . 0.671794^^^ |
|
| Interacting proteins: P60896 and P51587 |
Pubmed |
SVM Score :0.60263706 |
| Human DSS 1 associates with BRCA 2 , a tumour suppressor protein required for efficient recombinational DNA repair , but the biochemical function of DSS 1 is not known . 0.60263706^^^ |
|
| Interacting proteins: P60896 and P51587 |
Pubmed |
SVM Score :0.63308317 |
| Interaction between Arabidopsis Brca 2 and its partners Rad 51 , Dmc 1 , and Dss 1 . 0.63308317^^^ |
|
| Interacting proteins: P60896 and P51587 |
Pubmed |
SVM Score :0.0 |
| Interaction between the product of the breast cancer susceptibility gene BRCA 2 and DSS 1 , a protein functionally conserved from yeast to mammals . ^^^ Using the yeast two hybrid system with fragments of human BRCA 2 , we identified an interaction with the human DSS 1 ( deleted in split hand / split foot ) gene . ^^^ Yeast and mammalian two hybrid assays showed that DSS 1 can associate with BRCA 2 in the region of amino acids 2472 to 2957 in the C terminus of the protein . ^^^ Using coimmunoprecipitation of epitope tagged BRCA 2 and DSS 1 cDNA constructs transiently expressed in COS cells , we were able to demonstrate an association . ^^^ Furthermore , endogenous BRCA 2 could be coimmunoprecipitated with endogenous DSS 1 in MCF 7 cells , demonstrating an in vivo association . ^^^ |
|
| Interacting proteins: P60896 and P51587 |
Pubmed |
SVM Score :0.0 |
| Here , we present the 3 . 1 angstrom crystal structure of a approximately 90 kilodalton BRCA 2 domain bound to DSS 1 , which reveals three oligonucleotide binding ( OB ) folds and a helix turn helix ( HTH ) motif . ^^^ |
|
| Interacting proteins: P60896 and P51587 |
Pubmed |
SVM Score :0.0 |
| DSS 1 , the mammalian homologue of Sem1p , is functionally conserved between yeast and mammalian cells , and in mammalian cells physically interacts with the strong tumour supressor BRCA 2 . ^^^ |
|
| Interacting proteins: P60896 and P51587 |
Pubmed |
SVM Score :0.0 |
| The BRCA 2 interacting protein DSS 1 is vital for DNA repair , recombination , and genome stability in Ustilago maydis . ^^^ DSS 1 encodes a small acidic protein shown in recent structural studies to interact with the DNA binding domain of BRCA 2 . ^^^ Here we report that an ortholog of DSS 1 is present in Ustilago maydis and associates with Brh 2 , the BRCA 2 related protein , thus recapitulating the protein partnership in this genetically amenable fungus . ^^^ These findings conclusively show that Dss 1 constitutes a protein with a significant role in the recombinational repair pathway in U . maydis , and imply that it plays a similar key role in the recombination systems of organisms in which recombinational repair is BRCA 2 dependent . . ^^^ |
|
| Interacting proteins: P60896 and P51587 |
Pubmed |
SVM Score :0.0 |
| The activity of BRCA 2 is in turn governed by DSS 1 , a small acidic protein that appears to function as a necessary cofactor . ^^^ A model fungal system that reproduces the BRCA 2 RAD51 interaction offers the opportunity to understand the mechanism of DSS 1 activation at the molecular level . . ^^^ |
|
| Interacting proteins: P60896 and P51587 |
Pubmed |
SVM Score :0.0 |
| Overproduction of one such factor , Dsk 2 , is lethal in Saccharomyces cerevisiae and we show here that this lethality can be suppressed by mutations in SEM 1 , a gene previously recognized as an ortholog of the human gene encoding DSS 1 , which binds the BRCA 2 DNA repair protein . ^^^ |
|
| Interacting proteins: P60896 and P51587 |
Pubmed |
SVM Score :0.0 |
| DSS 1 is known to associate with the tumor suppressor protein BRCA 2 involved in repair of DNA double strand breaks ( DSBs ) . ^^^ |
|
| Interacting proteins: P60896 and P51587 |
Pubmed |
SVM Score :0.0 |
| Brh 2 , the BRCA 2 homolog in Ustilago maydis , functions in recombinational repair of DNA damage by regulating Rad 51 and is , in turn , regulated by Dss 1 . ^^^ |
|
| Interacting proteins: P60896 and P51587 |
Pubmed |
SVM Score :0.0 |
| Other groups have also shown how BRCA 2 , RAD 51 and DSS 1 co exist in a mnage trois and how the disruption of any one of the three cohabitants can have disastrous consequences for the cell . . ^^^ |
|
| Interacting proteins: P60896 and P51587 |
Pubmed |
SVM Score :0.0 |
| DSS 1 is required for the stability of BRCA 2 . ^^^ DSS 1 is an evolutionarily conserved acidic protein that binds to BRCA 2 . ^^^ Here , we developed a modified Western blotting protocol that detects endogenous DSS 1 protein , and used it to study the function of DSS 1 and its interaction with BRCA 2 in mammalian cells . ^^^ We found that essentially all BRCA 2 in human cell lines is associated with DSS 1 . ^^^ Importantly , we found that RNAi knockdown of DSS 1 in human cell lines led to dramatic loss of BRCA 2 protein , mainly due to its increased degradation . ^^^ |
|
| Interacting proteins: P60896 and P51587 |
Pubmed |
SVM Score :0.0 |
| NA |
|