Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P06730 and P60842	Pubmed	SVM Score :1.3319089
In eukaryotes protein synthesis initiates with the binding of the multimeric translation initiation complex eIF4F eIF4E , eIF4A and eIF4G to the monomethylated cap present on the 5 ' end of mRNAs . eIF4E interacts directly with the cap nucleotide , while eIF4A is a highly conserved RNA helicase and eIF4G acts as a scaffold for the complex with binding sites for both eIF4E and eIF4A . eIF4F binding to the mRNA recruits the small ribosomal subunit to its 5 ' end . 1.3319089^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.71166804
The association of eIF4A with Saccharomyces cerevisiae eIF4F has not yet been demonstrated , and therefore the degree to which eIF4A ' s conserved function relies upon this association has remained unclear . 0.71166804^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
The apparent fluctuations in the translation efficiency of rp mRNAs are accompanied by parallel changes in the expression of the genes encoding the initiation factors eIF 4E and eIF 4A . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Western blot analysis also showed that the protein levels of the three eIFs were differentially increased . eIF 4A protein levels increased in proportion to the observed increase in cellular protein synthetic activity while the increases in eIF 4E and eIF 2 alpha proteins were proportionately less . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
In mammalian cells this factor is composed of the cap binding protein eIF 4E , eIF 4A , and a 220 kDa polypeptide . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Immunological analysis revealed that the p 220 component of eIF 4F was decreased in extracts of AS cells and undetectable in AS cells treated with inducer , suggesting that p 220 and eIF 4E levels are coordinately regulated . eIF 4A , another component of eIF 4F , was unaltered . . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
The cap binding property of eIF 2 does not conflict with the current mRNA binding model of initiation factors eIF 4A , 4B , and 4F : cross linking of eIF 4E and of eIF 4B is stimulated by eIF 2 . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
The results indicate that eIF 3 and eIF 4F bind to the cap structure , possibly in the form of a complex , and that a modified form of eIF 3 alone has some cap binding activity in the complete absence of p 220 , eIF 4A , and eIF 4E . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Whereas all mRNAs tested required eIF 4A , mRNAs devoid of secondary structure in their 5 ' untranslated region did not require exogenous eIF 4E for translation . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Messenger RNAs encoding chloramphenicol acetyltransferase ( CAT ) with or without the 5 ' leader sequence of tobacco mosaic virus ( TMV ) RNA were synthesized in vitro and translated in Saccharomyces cerevisiae extracts dependent on eukaryotic initiation factors eIF 4E or eIF 4A . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
The equilibrium constants for the formation of the binary protein : m7GpppG , protein : mRNA , and protein : protein complexes as well as the ternary mRNA : eIF 4E : eIF 4A complexes were measured . ^^^ These studies show , for the first time , direct evidence for an eIF 4A : eIF 4E interaction . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
The interactions of protein synthesis initiation factors eIF 4E from human erythrocytes and eIF 4A and eIF 4F from rabbit reticulocytes with the cap analogue m7GpppG and rabbit globin mRNA were investigated . ^^^ The equilibrium binding constants for the binary complex formation of eIF 4E eIF 4A , m7GpppG eIF 4E , m7GpppG eIF 4F , globin mRNA eIF 4E , globin mRNA eIF 4F , and globin mRNA eIF 4A were measured by direct fluorescence titration experiments . ^^^ Association equilibrium constants were determined for the ternary system mRNA eIF 4E eIF 4A ; four thermodynamically independent equilibria characterize the system . ^^^ These equilibrium binding constants were used to calculate coupling free energies , which provided an estimate of the cooperativity of the interaction of eIF 4E , eIF 4A , and mRNA . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
The m7G affinity purified sample contains three major proteins , p 220 , eIF 4A , and p 28 ( also known as CBP 1 or eIF 4E ) . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Several initiation factors ( eIF 2 , eIF 3 , eIF 4A , eIF 4B , eIF 4C , eIF 4E and eIF 5 ) and elongation factor 1 were found to have no such discriminatory effect . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Cap dependent binding of mRNA to the 40 S ribosomal subunit during translational initiation requires the association of eukaryotic initiation factor 4G ( eIF4G ; formerly eIF 4 gamma and p 220 ) with other initiation factors , notably eIF4E , eIF4A , and eIF 3 . ^^^ Rhinovirus 2A protease and foot and mouth disease virus L protease were used to analyze the association of eIF4G with eIF4A , eIF4E , and eIF 3 . ^^^ Both proteases bisect eIF4G into N and C terminal fragments termed cpN and cpC . cpN was shown to contain the eIF4E binding site , as judged by retention on m7GTP Sepharose , whereas cpC was bound to eIF 3 and eIF4A , based on ultracentrifugal co sedimentation . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Canonical translation factors exhibited slight ( eIF 2 and GEF ) or no ( eIF 4A , eIF 4B , eIF 4E , eIF 4F , eIF 3 , and eEF 1 alpha ) stimulatory activity on translation of TAR containing CAT mRNA . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Stem loop structures in the leader region did not influence the binding of eIF 4E or , in comparative experiments , of eIF 4A . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
This process is mediated by the eukaryotic initiation factor 4 ( eIF 4 ) group of translation initiation factors consisting of eIF 4E , eIF 4A , eIF 4B , and eIF 4 gamma . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
The rate limiting step of eukaryotic protein synthesis is the binding of mRNA to the 40 S ribosomal subunit , a step which is catalyzed by initiation factors of the eIF 4 ( eukaryotic initiation factor 4 ) group : eIF 4A , eIF 4B , eIF 4E , and eIF 4 gamma . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Phosphorylation of the repressor decreases its affinity for eIF 4E , and thus relieves translational inhibition . eIF 4E forms a complex with two other polypeptides , eIF 4A and p 220 , that promote 40S ribosome binding to mRNA . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
These processes are both mediated by eukaryotic initiation factor 4F ( eIF4F ) , which consists of eIF4A ( helicase ) , eIF4E ( cap binding protein ) , and eIF4G subunits . ^^^ In an initiation reaction reconstituted in vitro from purified translation components and lacking eIF4A and 4F , IRES mediated initiation did not require the cap binding protein eIF4E but was absolutely dependent on eIF4A and the central third of eIF4G . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Cross linking experiments identified S . pombe eIF4E as the major cap binding protein while a further protein , p 36 , also showed cap dependent binding . eIF4A was not associated with the cap binding complex . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
The N terminal cleavage product of eIF4G ( cpN ) , which binds eIF4E , was completely degraded within 6 12 h , while the C terminal cleavage product ( cpC ) , which binds to eIF 3 and eIF4A , was more stable over the same period . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
It has homology to the carboxy terminal portion of the eukaryotic translation initiation factor ( eIF ) 4G that binds eIF4A and eIF4E to form eIF4F . ^^^ NAT 1 binds eIF4A but not eIF4E and inhibits both cap dependent and cap independent translation . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Eukaryotic translation initiation factor 4F ( eIF4F ) is a cap binding protein complex that consists of three subunits : eIF4A , eIF4E and eIF4G . eIF4G interacts directly with eIF4E and eIF4A . ^^^ The binding site of eIF4E resides in the N terminal third of eIF4G , while eIF4A and eIF 3 binding sites are present in the C terminal two thirds . ^^^ The GUG initiated open reading frame ( 907 amino acids ) has no canonical eIF4E binding site . p 97 binds to eIF4A and eIF 3 , but not to eIF4E . ^^^ These results suggest that p 97 functions as a general repressor of translation by forming translationally inactive complexes that include eIF4A and eIF 3 , but exclude eIF4E . . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
The foot and mouth disease virus encodes two forms of a cysteine proteinase ( L protease ) which bisects the eIF4G polypeptide into an N terminal fragment containing the eIF4E binding site , and a C terminal fragment which contains binding sites for eIF4A and eIF 3 and which associates with the 40S ribosomal subunit . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Mammalian translation initiation factor 4F ( eIF4F ) consists of three subunits , eIF4A , eIF4E , and eIF4G . eIF4G interacts directly with both eIF4A and eIF4E . ^^^ The binding site for eIF4E is contained in the amino terminal third of eIF4G , while the binding site for eIF4A was mapped to the carboxy terminal third of the molecule . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Mammalian eukaryotic translation initiation factor 4F ( eIF4F ) is a cap binding protein complex consisting of three subunits : eIF4E , eIF4A , and eIF4G . ^^^ Far Western analysis and coimmunoprecipitation experiments were used to demonstrate that eIF4GII directly interacts with eIF4E , eIF4A , and eIF 3 . eIF4GII , like eIF4GI , is also cleaved upon picornavirus infection . eIF4GII restores cap dependent translation in a reticulocyte lysate which had been pretreated with rhinovirus 2A to cleave endogenous eIF4G . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
With treatment of Saccharomyces cerevisiae with rapamycin or with entry of cells into the diauxic phase , eIF4G is rapidly degraded , whereas initiation factors eIF4E and eIF4A remain stable . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
In the initiation of translation in eukaryotes , binding of the small ribosomal subunit to the messenger RNA results from recognition of the 5 ' cap structure ( m7GpppX ) of the mRNA by the cap binding complex eIF4F . eIF4F is itself a three subunit complex comprising the cap binding protein eIF4E , eIF4A , an ATP dependent RNA helicase , and eIF4G , which interacts with both eIF4A and eIF4E and enhances cap binding by eIF4E . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
It interacts directly with the cap binding protein eIF4E through its N terminal part , and binds eIF 3 and eIF4A through the central and C terminal region . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Also , significant levels of the translation initiation factors eIF 2alpha , eIF 4E and eIF 4A were maintained during the growth cycle . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
In addition , we show that the amount of poly ( A ) binding protein ( PABP ) present in eIF4F complexes decreases during rotavirus infection , even though eIF4A and eIF4E remain unaffected . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Unexpectedly , we find that the cap binding complex eIF4F ( comprising eIF4E , eIF4G , and eIF4A ) assembles even when IRP 1 is bound to the cap proximal IRE . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Human eukaryotic translation initiation factor 4E ( eIF4E ) binds to the mRNA cap structure and interacts with eIF4G , which serves as a scaffold protein for the assembly of eIF4E and eIF4A to form the eIF4F complex . eIF4E is an important modulator of cell growth and proliferation . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Eukaryotic initiation factor 4A ( eIF4A ) is an RNA dependent ATPase and ATP dependent RNA helicase that is thought to melt the 5 ' proximal secondary structure of eukaryotic mRNAs to facilitate attachment of the 40S ribosomal subunit . eIF4A functions in a complex termed eIF4F with two other initiation factors ( eIF4E and eIF4G ) . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Translation of most mRNAs requires formation of a cap binding initiation complex known as eIF4F , consisting of factors eIF4E , eIF4A , eIF4E kinase Mnk 1 , poly ( A ) binding protein , and adaptor protein eIF4G . ^^^ Hsp 27 specifically bound eIF4G during heat shock , preventing assembly of the cap initiation / eIF4F complex and trapping eIF4G in insoluble heat shock granules . eIF4G is a specific target of Hsp 27 , as eIF4E , eIF4A , Mnk 1 , poly ( A ) binding protein , eIF4B , and eIF 3 were not bound by Hsp 27 and were not recruited into insoluble complexes . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Functions of the constituent proteins of eIF4F include recognition of the mRNA 5 ' cap structure ( eIF4E ) , delivery of an RNA helicase to the 5 ' region ( eIF4A ) , bridging of the mRNA and the ribosome ( eIF4G ) , and circularization of the mRNA via interaction with poly ( A ) binding protein ( eIF4G ) . eIF 4 activity is regulated by transcription , phosphorylation , inhibitory proteins , and proteolytic cleavage . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Translation of cellular mRNAs involves formation of a cap binding translation initiation complex known as eIF4F , containing phosphorylated cap binding protein eIF4E , eIF4E kinase Mnk 1 , eIF4A , poly ( A ) binding protein and eIF4G . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Affinity chromatography on m7GTP Sepharose shows that M FAG retains the ability of eIF4GI to associate with both the mRNA cap binding protein eIF4E and initiation factor eIF4A and that the ribosome bound form of M FAG is also present as a complex with eIF4E and eIF4A . ^^^ These data suggest that the binding sites for eIF4E , eIF4A and eIF 3 on eIF4GI are retained in the caspase generated fragment . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
The m7GpppN cap binding subunit eIF4E binds the N terminal region of eIF4G , which in turn contacts eIF4A through its central and C terminal regions . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Cap dependent translation is mediated by eIF4F , a protein complex composed of three subunits as follows : eIF4E , which recognizes the mRNA 5 ' cap structure ; eIF4A , an RNA helicase ; and eIF4G , a scaffolding protein that binds eIF4E , eIF4A , and the eIF4E kinase Mnk 1 simultaneously . eIF4E is hypophosphorylated and cap dependent translation is reduced at mitosis . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
The translational advantage conferred by the TEV IRES under these conditions was lost when the lysate reduced in eIF4F and eIFiso4F was supplemented with eIF4F ( or , to a lesser extent , eIFiso4F ) but not when supplemented with eIF4E , eIFiso4E , eIF4A , or eIF4B . eIF4G , the large subunit of eIF4F , was responsible for the competitive advantage conferred by the TEV IRES . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
The mammalian eukaryotic initiation factor 4GI ( eIF4GI ) may be divided into three roughly equal regions ; an amino terminal one third ( amino acids [ aa ] 1 to 634 ) , which contains the poly ( A ) binding protein ( PABP ) and eIF4E binding sites ; a middle third ( aa 635 to 1039 ) , which binds eIF4A and eIF 3 ; and a carboxy terminal third ( aa 1040 to 1560 ) , which harbors a second eIF4A binding site and a docking sequence for the Ser / Thr kinase Mnk 1 . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Translation initiating ribosome assembly at cap or IRES elements is mediated by a multiprotein complex of which the initiation factor 4F ( eIF4F ) consisting of eIF4A ( helicase ) , eIF4E ( cap binding protein ) , and eIF4G is a major constituent . eIF4G is a key target of picornaviral protease 2A , which cleaves this initiation factor into eIF4G ( Delta ) and ( Delta ) eIF4G to redirect the cellular translation machinery exclusively to its own IRES containing transcripts . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Eukaryotic initiation factor 4G ( eIF4G ) promotes mRNA recruitment to the ribosome by binding to the mRNA cap and poly ( A ) tail binding proteins eIF4E and Pap1p . eIF4G also binds eIF4A at a distinct HEAT domain composed of five stacks of antiparallel alpha helices . ^^^ In addition , excess eIF 1 inhibits growth of a second eIF4G mutant defective in eIF4E binding , which was also reversed by co overexpression of eIF4A . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Together with eIF4E and eIF4A , eIF4G constitutes the eIF4F complex which is a key component in promoting ribosome binding to the mRNA . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
PH promoted the assembly of eukaryotic initiation factor ( eIF ) 4F complexes consisting of eIF4E , eIF4G , eIF4A1 , and poly A binding protein . eIF4F complex formation after PH occurred without detectable changes in eIF4E binding protein 1 ( 4E BP 1 ) phosphorylation or its binding eIF4E . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
The eukaryotic translation initiation factor 4F ( eIF4F ) consists of three polypeptides ( eIF4A , eIF4G , and eIF4E ) and is responsible for recruiting ribosomes to mRNA . eIF4E recognizes the mRNA 5 ' cap structure ( m7GpppN ) and plays a pivotal role in control of translation initiation , which is the rate limiting step in translation . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
To date , the role of each of the four eIF 4 , i . e . eIF4E , eIF4G , eIF4A and eIF4B , is well established . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Translation of m7G capped cellular mRNAs is initiated by recruitment of ribosomes to the 5 ' end of mRNAs via eukaryotic translation initiation factor 4F ( eIF4F ) , a heterotrimeric complex comprised of a cap binding subunit ( eIF4E ) and an RNA helicase ( eIF4A ) bridged by a scaffolding molecule ( eIF4G ) . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
The helicase eIF4A was the most abundant eIF of the yeast cell , followed by eIF4E at multiple copies per ribosome , and eIF4B at approximately one copy per ribosome . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
BACKGROUND : The recruitment of mRNA for translation involves the assembly at the 5 ' cap of a complex of three initiation factors : the cap binding protein eIF4E , the ATP dependent RNA helicase eIF4A and the scaffold protein eIF4G . eIF4G mediates the binding of this mRNA protein complex to the 43S ribosomal preinitiation complex . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Recruitment of the 40S ribosome to the 5 ' end of a eukaryotic mRNA requires assembly of translation initiation factors eIF4E , the cap binding protein , together with eIF4A and eIF4G into a complex termed eIF4F . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Here , we report the selective binding of TIAR to several mRNAs encoding translation factors such as eukaryotic initiation factor 4A ( eIF4A ) and eIF4E ( translation initiation factors ) , eEF1B ( a translation elongation factor ) , and c Myc ( which transcriptionally controls the expression of numerous translation regulatory proteins ) . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
In higher eukaryotes , the C terminus of eIF4G ( 4G / C ) supports translational regulation by recruiting eIF4A , an RNA helicase , and Mnk 1 , the kinase responsible for phosphorylating eIF4E . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Interactions of eIF4G with eIF4E , eIF4A , eIF 3 , poly ( A ) binding protein , and Mnk1 / 2 have been mapped to discrete domains on eIF4G , and conversely , the eIF4G binding sites on all but one of these ligands have been determined . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
This interaction is mediated by a ribonucleoprotein network that contains , at a minimum , the poly ( A ) binding protein ( PABP ) , the capbinding protein eIF4E and a scaffolding protein , eIF4G . eIF4G , in turn , contains binding sites for eIF4A and eIF 3 , a 40S ribosome associated initiation factor . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
The activities of eIF4F , CBP 1 and the eIF4A free complex purified here were compared in a fractionated system translating capped globin mRNA . ^^^ They suggest that the eIF4A free complex may provide a function that is not a property of either CBP 1 or of eIF4A , but may be a property of p220 . . ^^^ The active complex therefore differs from eIF4F , the complex purified by cap analog affinity chromatography , in that it lacks the Mr 50 , 000 subunit which is antigenically identical to eIF4A . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Most initiation factor polypeptides are moderately abundant proteins with concentrations approaching those of ribosomes . eIF4A and eIF5A are more abundant than ribosomes , whereas eIF4F alpha and eIF2B are considerably less abundant than the other factors . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
The binding of mRNA to the ribosome is mediated by eukaryotic initiation factors eukaryotic initiation factor 4F ( eIF4F ) , eIF4B , eIF4A , and eIF 3 , eIF4F binds to the mRNA cap structure and , in combination with eIF4B , is believed to unwind the secondary structure in the 5 ' untranslated region to facilitate ribosome binding . eIF 3 associates with the 40S ribosomal subunit prior to mRNA binding . eIF4B copurifies with eIF 3 and eIF4F through several purification steps , suggesting the involvement of a multisubunit complex during translation initiation . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
After a brief introduction to the function of the mRNA specific translation factors eIF4A , eIF4B , and EIF4F , this article presents appropriate methodology for the study of the translation factors associated with the activation of mRNA for translation in eukaryotic systems . ^^^ The purification of eIF4A , eIF4B , and eIF4F from rabbit reticulocyte lysates is given along with a procedure for the purification of hemoglobin mRNA . ^^^ It is hoped that this information will facilitate the study of either the regulation of factor activity ( for eIF4A , eIF4B , or eIF4F ) or the translation efficiency ( sometimes regulated ) of various mRNAs . . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Ribosomal binding and positioning on these mRNAs did not require the initiation factors eIF 3 , eIF4A , eIF4B , and eIF4F and translation of these mRNAs was not inhibited by a trans dominant eIF4A mutant . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
This protein has been purified to greater than 80 % homogeneity from rabbit reticulocyte lysate and has been given the name eIF4H . eIF4H was shown to stimulate the in vitro activities of eIF4B and eIF4F in globin synthesis , as well as the in vitro RNA dependent ATPase activities of eIF4A , eIF4B , and eIF4F . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
In their absence , 43S ribosomal preinitiation complexes incubated with ATP , eIF4A , eIF4B and eIF4F bind exclusively to the cap proximal region but are unable to reach the initiation codon . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Ribosomal binding and positioning on this mRNA to form a 48S complex did not require the initiation factors eIF4A , eIF4B , or eIF4F , and translation of this mRNA was resistant to inhibition by a trans dominant eIF4A mutant that inhibited cap mediated initiation of translation . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Furthermore , eIF4A ( 46 kDa ) , a second component of eIF4F , is also cleaved in these later stages of the infection cycle . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
In contrast to mammalian eIF4B and eIF4A , the combination of wheat eIF4B and eIF4A does not stimulate RNA dependent ATP hydrolysis activity ; however , wheat eIF4B does stimulate eIF4F and eIF4A RNA dependent ATP hydrolysis activity . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
The resulting complex requires eIF 1 , eIF1A , eIF4A , eIF4B and eIF4F to bind to a messenger RNA and to scan to the initiation codon . eIF 5 stimulates hydrolysis of eIF 2 bound GTP and eIF 2 is released from the 48S complex formed at the initiation codon before it is joined by a 60S subunit to form an active 80S ribosome . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
These results suggest that , in terms of the scanning model of translation initiation , PABP may enhance the mRNA scanning rate of the complex formed by eIF4A , eIF4B , and eIF4F or eIF ( iso ) 4F and increase the rate of translation . . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
To be active , it must bind eIF4G and form the eIF4F complex , which also contains eIF4A . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
The RNA duplex unwinding activity of wheat germ eIF4A is similar to other mammalian systems ; however , eIF4F or eIFiso4F is required , probably because of the low binding affinity of wheat germ eIF4A for mRNA . ^^^ The data presented in this paper suggest that eIF4F or eIFiso4F acts to position the eIF4A and stabilize the interaction with mRNA . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Reconstitution of initiation using fully fractionated translation components indicated that 48S complex formation on both IRESs requires eIF 2 , eIF 3 , eIF4A , eIF4B , eIF4F , and the pyrimidine tract binding protein ( PTB ) but that the FMDV IRES additionally requires ITAF ( 45 ) , also known as murine proliferation associated protein ( Mpp 1 ) , a proliferation dependent protein that is not expressed in murine brain cells . ^^^ Specific binding sites for ITAF ( 45 ) , PTB , and a complex of the eIF4G and eIF4A subunits of eIF4F were mapped onto the FMDV IRES , and the cooperative function of PTB and ITAF ( 45 ) in promoting stable binding of eIF4G / 4A to the IRES was characterized by chemical and enzymatic footprinting . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
The cap binding complex eIF4F and the factors eIF4A and eIF4B are required for binding of 43S complexes ( comprising a 40S subunit , eIF2 / GTP / Met tRNAi and eIF 3 ) to the 5 ' end of capped mRNA but are not sufficient to promote ribosomal scanning to the initiation codon . eIF1A enhances the ability of eIF 1 to dissociate aberrantly assembled complexes from mRNA , and these factors synergistically mediate 48S complex assembly at the initiation codon . ^^^ The eIF4A and eIF4G subunits of eIF4F bind immediately upstream of the EMCV initiation codon and promote binding of 43S complexes . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Modulation of the helicase activity of eIF4A by eIF4B , eIF4H , and eIF4F . ^^^ This study demonstrates how the helicase activity of eIF4A is modulated by eIF4B , eIF4H , or as a subunit of eIF4F . ^^^ Results indicate that a linear relationship exists between the initial rate or amplitude of unwinding and duplex stability for all factor combinations tested . eIF4F , like eIF4A , behaves as a non processive helicase . ^^^ Furthermore , eIF4A ( or eIF4F ) becomes a slightly processive helicase in the presence of eIF4B or eIF4H . ^^^ The combinations of eIF4A , eIF4A + eIF4B , eIF4A + eIF4H , and eIF4F showed differences in their ability to unwind chemically modified duplexes . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
We found that a ribosomal 40S subunit , eukaryotic initiation factor ( eIF ) 3 , and the eIF 2 ternary complex form a 43S complex that can bind to the 5 ' end of an unstructured 5 ' untranslated region ( 5 ' UTR ) and in the presence of eIF 1 scan along it and locate the initiation codon without a requirement for adenosine triphosphate ( ATP ) or factors ( eIF4A , eIF4B , eIF4F ) associated with ATP hydrolysis . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Recombinant Pdcd 4 specifically inhibited the helicase activity of eIF4A and eIF4F . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
The extreme sensitivity to secondary structure around the initiation codon is likely to be due to the fact that the eIF4F complex ( which has eIF4A as one of its subunits ) is not required for and does not participate in initiation on these IRESs . . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
A critical test was provided by an mRNA with an unstructured 5 ' UTR , which is translated by scanning but does not absolutely need eIF4G and eIF4A : There was efficient reinitiation in a standard reticulocyte lysate , when initiation would be largely driven by eIF4F , but no reinitiation in an eIF4G depleted lysate . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
In addition , a complex containing bacterially expressed Vhs and a glutathione S transferase ( GST ) eIF4H fusion protein has RNase activity . eIF4H shares a region of sequence homology with eIF4B , and it appears to be functionally similar in that both stimulate the RNA helicase activity of eIF4A , a component of the mRNA cap binding complex eIF4F . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
The translation initiation factor eIF4A is the prototypical DEAD box RNA helicase that works in conjunction with eIF4B and eIF4H and as a subunit of eIF4F to prepare the mRNA template for ribosome binding , possibly by unwinding the secondary structure proximal to the 5 ' m7GpppN cap structure . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
In addition , eIF1A , eIF4F ( or the C terminal fragment of eIF4G ) , and eIF4A strongly stimulated the assembly of this complex , whereas eIF4B had no effect . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
This model can explain the cooperativity between all binding partners of eIF4A ( eIF4G , RNA , ATP ) and stimulation of eIF4A activity in the eIF4F complex . . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
Here we have investigated the role of two Trypanosoma brucei homologues of the translation initiation factor eIF4A ( in the light of subsequent experiments these were named as TbEIF4AI and TbEIF4AIII ) . eIF4A , a DEAD box RNA helicase , is a subunit of the translation initiation complex eIF4F which binds to the cap structure of eukaryotic mRNA and recruits the small ribosomal subunit . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
The eukaryotic translation initiation factor ( eIF ) 4B promotes the RNA dependent ATP hydrolysis activity and ATP dependent RNA helicase activity of eIF4A and eIF4F during translation initiation . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
We also demonstrate that both FCV and MNV RNA translation require the RNA helicase component of the eIF4F complex , namely eIF4A , because translation was sensitive ( albeit to different degrees ) to a dominant negative form and to a small molecule inhibitor of eIF4A ( hippuristanol ) . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
We have identified the initiation factor 4A ( eIF4A ) in a two dimensional protein database of Drosophila wing imaginal discs . eIF4A , a member of the DEAD box family of RNA helicases , forms the active eIF4F complex that in the presence of eIF4B and eIF4H unwinds the secondary structure of the 5 ' UTR of mRNAs during translational initiation . ^^^
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
NA
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
NA
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
NA
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
NA
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
NA
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
NA
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
NA
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
NA
Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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Interacting proteins: P06730 and P60842	Pubmed	SVM Score :0.0
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