Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
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Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.53263222
Iodinated PLP was found to bind MBP but not any other CNS myelin proteins . 0.53263222^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.62038866
T cell reactivities to the putative autoantigens myelin basic protein ( MBP ) , MBP peptides with amino acid residues 110 128 and 148 165 , and myelin proteolipid protein ( PLP ) were examined in patients with acute ischaemic cerebrovascular disease ( CVD ) and , for comparison , in patients with inflammatory neurological diseases and other neurological diseases . 0.62038866^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.82993637
Pronounced differences in occurrence and numbers of T cells recognizing MBP , MBP peptides with the amino acid sequences 63 88 , 110 128 and 148 165 , and PLP were noticed in individual ON and MS patients over the course of disease . 0.82993637^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.6956871
Following this step , two pathways may be activated : ( 1 ) induction of antigen specific suppressor T cells by determinants shared between MBP and copolymer 1 , or ( 2 ) competition with MBP and other myelin associated antigens , PLP and myelin oligodendrocyte glycoprotein , for the activation of effector T cells . 0.6956871^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Myelin basic protein is affected by reduced synthesis of myelin proteolipid protein in the jimpy mouse . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Myelin proteolipid protein from the same gel was racemized approximately half as much as MBP . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
It is noteworthy that alternative splicing of the genes for MBP and PLP also produced multiple mRNAs . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Susceptibility to EAE induced by either PLP or bovine myelin basic protein ( MBP ) also was reduced in old SJL mice . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
EAE is caused by CD4+ T cells specific for defined myelin protein , like MBP and PLP . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Antibodies against mammalian myelin basic protein ( MBP ) and proteolipid protein ( PLP ) were not recognized . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
In normal mice , maximal expression of the PLP gene lags behind that of the MBP gene by several days . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
In this study we detected MBP and PLP in the vaccine using immunological methods . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Myelin was immunostained with antibodies to either proteolipid protein ( PLP ) or myelin basic protein ( MBP ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Both myelin basic protein ( MBP ) and proteolipid protein ( PLP ) have been considered candidate autoantigens . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
They produce little myelin basic protein ( MBP ) , never immunostain for PLP , and rarely elaborate myelin like membranes . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Myelin compaction is mediated by MBP and by PLP , which fulfills some analogous functions in the CNS as PO in the PNS . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
These include MBP , PLP , MOG , MAG , CNP and S 100 . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Finally , the effect of non immune factors in resistance is illustrated by mice with mutations in the MBP or PLP gene . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Oligodendrocyte markers were expressed such as CNPase , MBP and proteolipid protein ( PLP and DM 20 ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
By contrast , the hormone had no effect on the levels of MBP or proteolipid protein ( PLP ) mRNA . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Moreover , increased CNP , and enhanced PLP and myelin basic protein expression were found after GLA administration . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The incorporation of tritium from NaB3H4 into myelin proteins was confirmed by reaction with purified components of myelin basic protein or with lipophilin , a purified fraction of proteolipid protein . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Transcription of the two genes during myelination . cDNA clones of rat brain proteolipid protein ( PLP ) , also named lipophilin , the major integral myelin membrane protein , and of myelin basic protein ( MBP ) , the major extrinsic myelin protein , have been isolated from a rat brain cDNA library cloned into the PstI site of pBR 322 . ^^^ Oligonucleotides synthesized according to the established amino acid sequence of lipophilin and the nucleotide sequence of the small myelin basic protein of the N terminal , the central and C terminal region of their sequences were used as hybridization probes for screening . ^^^ These clones were also characterized . pMBP 2 was sequenced and used together with the lipophilin cDNA clones as hybridization probes to estimate the lipophilin and myelin basic protein mRNA levels of rat brain during the myelination period . ^^^ The expression of the lipophilin and myelin basic protein genes during development of the myelin sheath appears to be strictly coordinated . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Interaction between human myelin basic protein and lipophilin . ^^^ The interaction of human myelin basic protein with lipophilin has been demonstrated by affinity chromatography . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Purified lipophilin , a hydrophobic lipoprotein of myelin , induces a cell mediated demyelinating disease of the central nervous system similar to experimental allergic encephalomyelitis ( EAE ) induced by the myelin basic protein ( MBP ) . ^^^ Guinea pigs challenged with lipophilin ( emulsified with CFA ) developed clinical and histological signs of disease indistinguishable from those developed by animals similarly challenged with MBP . ^^^ Both lipophilin and MBP induced and elicited delayed type hypersensitivity in animals challenged with respective antigens . ^^^ Tryptophan , an essential component of the MBP determinant for disease in guinea pigs , is required for the encephalitogenicity of lipophilin . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Myelin basic protein was shown to extend into cranial roots , in contrast to myelin proteolipid protein which abruptly lose fluorescence as the nerves emerged from the brain . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Experimental autoimmune encephalomyelitis ( EAE ) is an experimentally induced demyelinating disease mediated by CD4+ T cells specific for various myelin proteins including myelin basic protein ( MBP ) and myelin proteolipid protein ( PLP ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
T cell sensitization to two myelin components , myelin basic protein ( MBP ) and myelin proteolipid protein ( PLP ) , may be important to the pathogenesis of multiple sclerosis ( MS ) . ^^^ Frequency of T cells specific for myelin basic protein and myelin proteolipid protein in blood and cerebrospinal fluid in multiple sclerosis . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The level of myelin basic protein in the CNS is also intermediate ; however , myelin proteolipid protein ( PLP ) is virtually absent . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Low levels of mRNA coding for 2 ' , 3 ' cyclic nucleotide 3 ' phosphohydrolase and myelin proteolipid protein , but not for myelin basic protein , were present in the glial progenitor cells . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
OGF infused into the cerebral cortex of the adult rat accelerates the production of myelin proteins as shown by increased specific activity of the myelin enzyme 2 ' , 3 ' cyclic nucleotide 3 ' phosphohydrolase ( 2 ' , 3 ' CNPase ) , by stimulated synthesis of myelin basic protein , and by elevations in levels of myelin proteolipid protein RNA . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Gene expression for myelin proteolipid protein ( PLP ) and myelin basic protein ( MBP ) in the dysmyelinating mutant mice shiverer and jimpy was analyzed by nuclear run off transcription assay , in situ hybridization , and immunohistochemistry . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Electron microscopic immunocytochemical localization of myelin proteolipid protein and myelin basic protein to oligodendrocytes in rat brain during myelination . ^^^ Electron microscopic immunocytochemical studies were carried out to localize myelin basic protein and myelin proteolipid protein during the active period of myelination in the developing rat brain using antisera to purified rat brain myelin proteolipid protein and large basic protein . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Its proteins consisted of ( 1 ) two basic proteins ( 16 , 000 and 18 , 500 apparent Mr ) that reacted with anti human CNS myelin basic protein antibodies and ( 2 ) a major protein ( 29 , 000 apparent Mr ) that stained with concanavalin A horseradish peroxidase and bound to anti rat CNS myelin proteolipid protein ( PLP ) antibodies . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
We also examined the developmental pattern of the gene expression of MBP and that of another protein , myelin proteolipid protein ( PLP ) , specifically expressed in the oligodendrocyte , in mld by RNA dot blot study . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
These RNA preparations were analyzed by both Northern and dot blot for their content of 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNPase ) , myelin proteolipid protein ( PLP ) , and myelin basic protein ( MBP ) specific transcripts . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Computer generated `` hydropathic ' ' profiles were constructed for graphic comparison of the amino acid sequences for P 2 protein , 18 . 5 kilodalton ( kDa ) myelin basic protein ( BP ) , and myelin proteolipid protein ( PLP ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The myelin specific proteins , myelin basic protein ( MBP ) and myelin proteolipid protein ( PLP ) were quantitated by radioimmunoassay ( RIA ) and the activity of the enzyme 2 ' , 3 ' cyclic 3 ' phosphohydrolase ( CNP ) measured , in 27 regions of normal brain and spinal cord . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Using radioimmunoassays ( RIA ) for the myelin specific proteins , myelin proteolipid protein ( PLP ) and myelin basic protein ( MBP ) and an enzyme assay for the activity of the myelin marker enzyme 2 ' 3 ' cyclic 3 ' phosphohydrolase ( CNPase ) , we have studied plaque , periplaque and normal appearing white matter ( NAWM ) tissue . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
We investigated whether lacZ genes could be specifically controlled in mouse glioma cells by glial specific promoters , including the 2 . 5 kb 5 ' flanking region of the mouse glial fibrillary acidic protein ( GFAP ) gene , the 1 . 3 kb 5 ' flanking region of the myelin basic protein ( MBP ) gene , and the 1 . 5 kb 5 ' flanking region of the myelin proteolipid protein ( PLP ) gene . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The major involvement of the proximal PNS in autoimmune disease directed at MBP is in marked contrast to EAE induced by immunisation with myelin proteolipid protein , where the inflammation and demyelination are restricted to the CNS . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Proliferative responses to myelin basic protein but not to myelin proteolipid protein were present in peripheral blood lymphocytes of immunized animals . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Numbers of IL 2 secreting cells were higher in MS patients compared to patients with AM + TH after stimulation with myelin proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) and myelin oligodendrocyte glycoprotein ( MOG ) , but not after stimulation with myelin basic protein ( MBP ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Abnormal autoimmunity could play a role in the pathogenesis of multiple sclerosis ( MS ) , in analogy with its model experimental allergic encephalomyelitis ( EAE ) which can be transferred by T cell lines directed to myelin basic protein ( MBP ) and myelin proteolipid protein ( PLP ) , and worsened with antibody to myelin oligodendrocyte glycoprotein ( MOG ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Myelin basic protein ( MBP ) and myelin proteolipid protein ( PLP ) are both highly abundant in CNS myelin , but are also produced in Schwann cells . ^^^ Myelin basic protein ( MBP ) and myelin proteolipid protein ( PLP ) are both highly abundant in CNS myelin , but are also produced in Schwann cells . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Our approach involved a systematic epitope mapping of responses to myelin proteolipid protein ( PLP ) as well as assaying responses to known encephalitogenic determinants of myelin basic protein ( MBP 87 89 ) and myelin oligodendrocyte glycoprotein ( MOG 92 106 ) at various times after induction of EAE in ( SWR 10 SJL ) F 1 mice immunized with PLP 139 151 . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
By 7 8 weeks after immunization of ( SWR 10 SJL ) F 1 mice with the immunodominant myelin proteolipid protein determinant ( PLP 139 151 ) , splenocytes consistently respond to the immunodominant myelin basic protein determinant ( MBP 87 99 ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The amount of mRNAs of myelin basic protein and of 2 ' 3 ' cyclic nucleotide 3 ' phosphohydrolase were markedly increased in the experimental animals , whereas myelin proteolipid protein mRNA did not show differences relative to controls . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Using stringent criteria for recognition , none of the lines from MS affected or control subjects recognized intact myelin basic protein ( MBP ) or myelin proteolipid protein ( PLP ) molecules . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
We tested the proliferative responses of peripheral blood mononuclear cells from 61 patients with multiple sclerosis , 56 healthy control subjects and 52 patients with other neurological diseases to seven synthetic peptides of myelin proteolipid protein ( PLP ) and 19 synthetic peptides of myelin basic protein ( MBP ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
We have shown that the 1 . 3 kb fragment of the mouse myelin basic protein ( MBP ) promoter region initiates transcription in mouse glioma cells more efficiently than glial fibrillary acidic protein ( GFAP ) or myelin proteolipid protein ( PLP ) promoter . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Likewise , concentrations of myelin specific proteins ( MBP , myelin proteolipid protein , MAG , and 2 ' , 3 ' cyclic nucleotide , 3 ' phosphodiesterase ) were not significantly different in Igf 1 ( / ) and WT mice . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Expression of the mRNAs of myelin basic protein ( MBP ) myelin proteolipid protein ( PLP ) , 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNPase ) , transferrin , and c Jun was investigated in 10 and 17 day old normal and hyperthyroid rats , using Northern blot analysis . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
To investigate the role of molecular mimicry in the pathogenesis of postviral demyelination , alignment algorithms were used and amino acid homologies between immunogenic epitopes of SFV and myelin autoantigens , myelin basic protein ( MBP ) , myelin proteolipid protein ( PLP ) and myelin oligodendrocyte glycoprotein ( MOG ) were identified . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Human T cell lines established using intact or delipidated myelin as the antigen were highly heterogeneous in response , and were each able to recognize myelin basic protein and myelin proteolipid protein peptides . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
In the present study , we have optimized the NEPHGE 2D PAGE protocol for the analysis of myelin proteins with basic pIs , and provide a NEPHGE 2D PAGE map based on size , pI , and immunoreactivity ( Western blot ) of myelin basic protein ( MBP ) , 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNP ) , myelin proteolipid protein ( PLP ) , and its smaller spliced variant DM 20 , myelin / oligodendrocyte glycoprotein ( MOG ) and oligodendrocyte specific protein ( OSP ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Early pregnancy factor suppresses experimental autoimmune encephalomyelitis induced in Lewis rats with myelin basic protein and in SJL / J mice with myelin proteolipid protein peptide 139 151 . ^^^ Two models of EAE were investigated , acute EAE induced in Lewis rats by inoculation with myelin basic protein ( MBP EAE ) and chronic relapsing EAE induced in SJL / J mice by inoculation with myelin proteolipid protein peptide ( residues 139 151 ) ( PLP EAE ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Substantial biological data indicate that the myelin basic protein ( MBP ) and myelin proteolipid protein ( PLP / DM20 ) genes produce products with functions beyond that of serving as myelin structural proteins . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
These account for approximately 40 % of the amino acids of myelin proteolipid protein and for approximately 20 % of myelin basic protein . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Effect of cyclic AMP on the expression of myelin basic protein species and myelin proteolipid protein in committed oligodendrocytes : differential involvement of the transcription factor CREB . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Biochemical analyses of AD white matter combining size exclusion , normal phase , and gas chromatography , immunoassays , and Western blotting revealed increased quantities of Abeta 40 and Abeta 42 in AD white matter accompanied by significant decreases in the amounts of myelin basic protein , myelin proteolipid protein , and 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Even so , the effects on avidin linked lipids are different from those induced by myelin basic protein on freely diffusible lipids , both interacting with the myelin proteolipid protein . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
We have investigated the association of hsp 70 with myelin basic protein ( MBP ) , myelin proteolipid protein ( PLP ) and myelin oligodendrocyte protein ( MOG ) in MS and control brain tissue . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Oligodendroglial cultures were characterized by the expression of such cell type specific mRNAs as myelin proteolipid protein ( PLP ) , oligodendrocyte specific protein ( OSP ) , and 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNPase ) and were further characterized by immunostaining for such differentiation markers as myelin basic protein ( MBP ) , PLP , CNPase , and O 4 . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Over several years we have collected blood from MS patients and controls , and measured T cell responses to myelin proteolipid protein ( PLP ) and myelin basic protein ( MBP ) and have shown increased responses to PLP in MS patients compared to healthy controls and patients with other neurological diseases . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
METHODS : The expression of cyclic nucleotide phosphodiesterase , glial fibrillary acidic protein , myelin associated glycoprotein , myelin basic protein , and myelin proteolipid protein were assessed in the superior frontal gyrus and the primary motor cortex of control , uncomplicated alcoholic , and cirrhotic alcoholic cases . ^^^ RESULTS : Overall , the expression of cyclic nucleotide phosphodiesterase , glial fibrillary acidic protein , myelin associated glycoprotein , and myelin basic protein were significantly lower in the cirrhotic alcoholic cases compared with controls , with a similar tendency for myelin proteolipid protein . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Besides this role , Id 4 also has the ability to decrease the activity of specific myelin promoters , since Id 4 overexpression decreases the activity of luciferase reporter genes driven by the ceramide galactosyltransferase ( CGT ) or myelin basic protein ( MBP ) promoter , but not by a myelin proteolipid protein ( PLP ) promoter . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The present study examines the developmental expression of the major myelin protein genes and translatability of PLP and MBP mRNA . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
In this study , levels of myelin basic protein ( MBP ) and proteolipid protein ( PLP ) were measured by immunoblotting in samples prepared from spinal cord , brainstem and cerebral hemispheres of normal and affected goats at 2 4 days ( newborns ) and 2 4 weeks of age . ^^^ In affected goats , total levels of MBP in spinal cord were normal , while PLP levels were 60 70 % of normal at both ages . ^^^ In contrast , PLP and MBP in brainstem and cerebral hemispheres were severely decreased at both ages , with levels of PLP 10 13 % and MBP 25 29 % of normal in newborns , and generally more reduced at 2 4 weeks . ^^^ Myelin from spinal cord had a normal composition with regard to PLP and MBP content , while the myelin fraction from brainstem was markedly deficient in both proteins . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Tolerance induced by the i . v . injection of syngeneic splenocytes coupled with purified neuroantigens or encephalitogenic peptides of MBP and PLP using ethylene carbodiimide was extremely effective in both prevention and treatment of acute and relapsing forms of EAE in Lewis rats and SJL / J mice . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Peak ( day 21 ) proteolipid protein ( PLP ) and myelin basic protein ( MBP ) mRNA levels were reduced when pups were nursed by mothers fed a fat free or 5 % coconut oil diet . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Evaluation of T cell repertoire by enumeration of cells secreting interferon gamma in response to proteolipid protein ( PLP ) , myelin basic protein ( MBP ) , and to various synthetic MBP peptides revealed similar patterns of T cell reactivity within the families both in MS affected parents and unaffected children . ^^^ Analysis of B cell responses in blood showed low numbers of cells secreting IgG , IgA , or IgM antibodies against MBP , PLP , myelin associated glycoprotein , and myelin oligodendrocyte glycoprotein both in MS affected and unaffected family members . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Live labeled oligodendrocytes were stained brightly for myelin oligodendrocyte glycoprotein , galactocerebroside , and other surface markers but did not stain for the PLP C terminus or the intracellular proteins myelin basic protein and beta tubulin . ^^^ The permeabilized oligodendrocytes stained intensely for the PLP C terminus , myelin basic protein , and beta tubulin . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Immunostaining intensity is somewhat reduced for myelin basic protein ( MBP ) and the C terminal common to proteolipid protein ( PLP ) and DM 20 and profoundly decreased for the PLP specific peptide . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The initial onset of disease correlates with delayed type hypersensitivity ( DTH ) reactivity specific for PLP 139 151 ( S ) , in the apparent absence of T cell reactivity to the major myelin basic protein ( MBP ) peptide . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
These data indicate that encephalitogenic determinants of PLP are short contiguous peptide sequences similar in length and diversity to those of MBP . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Transcription rates of the PLP and MBP genes parallel their respective steady state mRNA levels throughout rat brain development . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Early myelination in zitter rat was investigated by light and electron microscopic observations , by immunostaining for myelin basic protein ( MBP ) , proteolipid protein ( PLP ) and myelin associated glycoprotein ( MAG ) , and by morphometric analysis from the 1st to the 28th day of age . ^^^ However , the fundamental structure of the myelin lamellae appeared to be normal and the immunoreactivities for MBP , PLP and MAG were slightly delayed and weakend in comparison with age matched controls . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Relative to myelin purified from the same starting material , the 0 . 8M subfraction was enriched in MAG , CNPase , carbonic anhydrase and Na+ , K+ ATPase but was extremely low in PLP and MBP . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
T 3 increases the concentrations of mRNA transcribed from the following four myelin protein genes : myelin basic protein ( Mbp ) , myelin associated glycoprotein ( Mag ) , proteolipid protein ( Plp ) , and 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( Cnp ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Changes in myelin gene expression were not limited to PLP mRNA , as the steady state levels of myelin basic protein ( MBP ) mRNA paralleled those of PLP mRNA in the developing cerebrum and in degenerating optic nerves . ^^^ Thus , oligodendrocytes require axons to maintain their normal levels of PLP and MBP transcripts and the high proportion of distally initiated PLP transcripts that characterize early myelination . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Our results confirm the extensive degradation of myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , and , to a lesser extent , the other myelin proteins by endo and exogenous CANP . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
In addition to myelin basic protein ( MBP ) and proteolipid protein ( PLP ) , oligodendrocyte ( Od ) membrane autoantigens , such as the glycoprotein M2 / MOG , could participate in the pathogenesis of autoimmune demyelinating diseases of the central nervous system ( CNS ) , such as experimental allergic encephalomyelitis ( EAE ) or multiple sclerosis ( MS ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
We have used this technique to detect and locate mRNA for myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNPase ) and myelin associated glycoprotein ( MAG ) in oligodendrocytes of 7 day old and adult rat brains . ^^^ PLP and MAG mRNA were restricted to the perinuclear cytoplasm , whereas MBP and CNPase mRNA was additionally present in peripheral oligodendrocyte processes . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Myelin basic protein , palmitoyl CoA hydrolase , and myelin associated nonspecific esterase were also ruled out as the PLP fatty acylesterase . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
T cell responses against intact , purified major myelin proteins ( myelin basic protein ( MBP ) and proteolipid protein ( PLP ] , and against altered myelin constituents were readily demonstrable in SJL / J mice with R EAE , but were not detectable in SJL / J mice with TMEV induced demyelinating disease . ^^^ TMEV infected mice also did not display T cell responses against the peptide fragments of MBP ( 91 104 ) and PLP ( 139 151 ) recently shown to be encephalitogenic in SJL / J mice . ^^^ In addition , induction of neuroantigen specific tolerance to a heterogeneous mixture of CNS antigens , via the i . v . injection of syngeneic SJL / J splenocytes covalently coupled with mouse spinal cord homogenate , resulted in significant suppression of clinical and histologic signs of R EAE and the accompanying MBP and PLP specific DTH responses . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Mice tolerized with splenocytes coupled with MSCH ( a complex mixture of neuroantigens ) or with purified PLP , but not purified myelin basic protein , were resistant to the development of clinical and histologic R EAE . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Plasmolipin levels in myelin were enriched during five successive cycles of myelin purification , similar to the enrichment of myelin proteolipid apoprotein ( PLP ) and myelin basic protein ( MBP ) . ^^^ Plasmolipin , purified from kidney membranes , was detected by silver stain on gels at 18 kDa and did not show immunological cross reactivity with either PLP or MBP . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The myelin deficient ( md ) rat shows greatly reduced mRNA expression for several oligodendrocyte markers : glycerol phosphate dehydrogenase ( GPDH ) , myelin basic protein ( MBP ) and proteolipid protein ( PLP ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
We determined the temporal profile of expression of the genes encoding the P 0 glycoprotein , the myelin associated glycoprotein ( MAG ) , the myelin basic protein ( MBP ) , the proteolipid protein ( PLP ) , and 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNP ) in the sciatic nerve of rats . ^^^ P 0 and MBP RNA were present at very high levels , whereas the amount of MAG , PLP and CNP RNA were much less . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
In situ hybridization studies of rat spinal cord sections localized mRNAs encoding proteolipid protein ( PLP ) and myelin basic protein ( MBP ) 20 and 40 days after unilateral rhizotomy . ^^^ Cellular RNA was extracted from transected and age matched control optic nerves 5 , 10 , 20 , and 40 days after surgery , and levels of the following mRNAs were determined by slot blot procedures : PLP , MBP , myelin associated glycoprotein ( MAG ) , and 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNP ) . ^^^ In transected nerves , PLP and MBP mRNA levels were approximately 85 % , 45 % , and 25 % of control values at 5 , 20 and 40 days posttransection , respectively . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Myelin basic protein ( BP ) , an encephalitogenic peptide of BP ( BP 87 99 ) , and proteolipid protein ( PLP ) can each induce R EAE in SJL / J mice , and a peptide of PLP ( PLP 139 151 ) has been reported to induce acute EAE . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
A nonradioactive in situ hybridization procedure was employed to study the distribution of mRNAs for myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) and 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNP ) in oligodendrocytes in primary cultures of mouse brain . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
To elucidate the molecular mechanisms producing reduced levels of PLP , myelin basic protein ( MBP ) and glycerol phosphate dehydrogenase ( GPDH ) mRNAs , and their corresponding proteins in md rats , in vitro transcription assays were performed . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Myelin basic protein ( MBP ) and proteolipid protein ( PLP ) gene expression was investigated in the murine dysmyelinating mutant , jimpy and age matched normal mice . ^^^ MBP and PLP mRNA expression was examined in several brain regions by in situ hybridization histochemistry between 10 and 20 days postpartum . ^^^ The results showed a general reduction in both PLP and MBP mRNA expression throughout in jimpy brain due primarily to fewer numbers of jimpy oligodendrocytes expressing these genes . ^^^ Myelin basic protein ( MBP ) and proteolipid protein ( PLP ) gene expression was investigated in the murine dysmyelinating mutant , jimpy and age matched normal mice . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Umbilical cord blood of newborns and peripheral blood of healthy adults were investigated by an immunospot assay for cells secreting IgG , IgA and IgM antibodies against myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) and myelin oligodendrocyte glycoprotein ( MOG ) which represent putative antigens for an autoimmune attack in multiple sclerosis ( MS ) and against acetylcholine receptor ( AChR ) which is considered an important autoantigen in myasthenia gravis . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
We note especially that there are potential nucleotide binding sites in proteolipid protein ( PLP ) , MBP and 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNP ) . ^^^ This is consistent with the experimental observations that PLP acts like an ionophore or proton channel when reconstituted into planar lipid bilayers , MBP binds GTP , and CNP catalyzes in vitro the hydrolysis of 2 ' , 3 ' nucleotides into corresponding 2 ' nucleotides . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The distribution of proteolipid protein ( PLP ) and myelin basic protein ( MBP ) was examined in differentiating oligodendrocytes of primary and secondary mouse brain cell cultures by single and double label indirect immunofluorescence . ^^^ In primary cultures , MBP and PLP were differentially located in oligodendrocytes . ^^^ To compare the detailed distribution of PLP and MBP in the absence of underlying cells , a simple method for the growth of isolated oligodendrocytes in secondary cultures was developed . ^^^ MBP and PLP were differentially localized in these cell structures . ^^^ The observations show that PLP and MBP are differentially located in cultured mouse oligodendrocytes . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The integral proteolipid apoprotein ( PLP ) from bovine spinal cord has been reconstituted in dimyristoylphosphatidylglycerol ( DMPG ) bilayers , and the mutual interactions on binding the peripheral myelin basic protein ( MBP ) have been studied . ^^^ Quantitation of protein and lipid contents in the MBP PLP DMPG double recombinants at different PLP : DMPG ratios led to the conclusion that MBP binds only to the DMPG lipid headgroups and is hindered from interaction with the first shell of lipids surrounding the PLP . ^^^ No specific PLP MBP association could be detected . ^^^ Electron spin resonance ( ESR ) spectra of phosphatidylglycerol spin labeled at position n = 5 in the sn 2 chain showed that complexation of MBP with the PLP DMPG recombinants leads to a decrease in lipid chain mobility to an extent which correlates with the degree of MBP binding . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Effects of dexamethasone ( DEX ) on the relative abundance of myelin basic protein ( MBP ) , proteolipid protein ( PLP ) and glial fibrillary acidic protein ( GFAP ) mRNAs in the developing rat brain were examined . ^^^ The appearance of MBP , PLP and GFAP mRNAs in cerebellum preceded that in cerebrum in the control group . ^^^ In cerebrum , the relative abundance of MBP and PLP mRNAs was significantly less in the DEX group than that in the control group at P 20 and P 30 . ^^^ In cerebellum , a significant decrease in the abundance of MBP , PLP and GFAP mRNAs in the DEX group was observed only at P 10 but not at P 20 and P 30 . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Haplotypes H 2s and H 2u appear to be immunologically co dominant in F 1 mice in the PLP EAE system , which differs from the H 2u dominance in F 1 mice in the myelin basic protein EAE system . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
However , activated , PLP reactive T cells were susceptible to lysis by cytotoxic MBP specific T cells in the presence of MBP . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
We have reexamined the levels of myelin basic protein ( MBP ) and proteolipid protein ( PLP ) in the brains of mice bred to carry both the shi / shi and jp / Y hypomyelination defects . ^^^ MBP and PLP levels were assessed by immunoblotting . ^^^ Animals proven to be shi / shi * jp / Y had no detectable MBP or PLP ; those proven to be shi / shi * + / Y ( the crossover ) had no MBP but had PLP . ^^^ These results differ from an earlier report of both MBP and PLP in the brains of presumed shi * jp animals . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Alternative splicing of the transcripts of the single genes of PLP and myelin basic protein ( MBP ) is the underlying mechanism by which the isoforms of the two main proteins of the myelin membrane arise . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Myelin protein composition of the sheaths was examined by immunostaining for myelin basic protein ( MBP ) and two peptide regions of PLP / DM 20 molecule ; one being proteolipid protein ( PLP ) specific and the other recognizing the c terminal common to PLP DM 20 . ^^^ The majority of myelin sheaths immunostained for MBP and PLP . ^^^ Occasional MBP positive sheaths failed to stain with PLP / DM 20 or PLP specific antiserum . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
MAP1B staining was also compared with that of myelin proteolipid ( PLP ) , myelin basic protein ( MBP ) and beta tubulin in secondary glial cultures that were enriched for oligodendrocytes . ^^^ The results showed a typical staining of cell bodies and membranous profiles using PLP antibodies , and the staining of cell bodies and flattened regions of membranous sheets by MBP antibodies . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
We also found that glucocorticoids stimulate the translation of MBP and PLP mRNAs and inhibit the translation of CNP mRNA in cell free systems . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
These findings demonstrate that immunization of SJL / J mice with PLP peptide 139 151 produces a disease with the clinical and morphologic features of CNS tissue , whole PLP , whole MBP , and MBP peptide induced acute EAE . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Incubation of myelin purified from rat spinal cord with CaCl 2 ( 1 5 mM ) in 10 50 mM Tris HCl buffer at pH 7 . 6 containing 2 mM dithiothreitol resulted in the loss of both the large and small myelin basic proteins ( MBPs ) , whereas incubation of myelin with Triton 10 100 ( 0 . 25 0 . 5 % ) and 5 mM EGTA in the absence of calcium produced preferential extensive loss of proteolipid protein ( PLP ) relative to MBP . ^^^ These results indicate that purified myelin is associated with and / or contains a CANP whose substrates include MBP , PLP , and 150K NFP . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
A chronic experimental allergic encephalomyelitis ( EAE ) was produced in Hartley guinea pigs with bovine white matter proteolipid protein ( PLP ) , in which the levels of myelin basic protein ( MBP ) and galactocerebroside ( GC ) were less than 0 . 014 % and 0 . 13 % , respectively , by our method of purification . ^^^ Cells of an MBP specific T cell line did not proliferate in the presence of 100 micrograms of PLP and antigen presenting cells . ^^^ Antibodies to PLP were highly elevated in the animals with demyelinating plaques but antibodies to MBP and GC were not elevated in the serum samples . ^^^ These results suggest that PLP is encephalitogenic and produces demyelination in the central nervous system without contamination by MBP or GC in Hartley guinea pigs . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Almost no MBP , and low levels of PLP , were detected in the immunoblots , suggesting the possibility of a partial failure of PLP to be assembled into myelin in shiverer . ^^^ In the jimpy mutant , low levels of MBP synthesis were observed in vitro ( approximately 26 % of controls ) and very little synthesis of PLP was evident . ^^^ The immunoblots of 21 day jimpy brain homogenates revealed no appreciable steady state levels of PLP or MBP , again indicating that most newly synthesized MBPs were not incorporated into a stable membrane structure in this mutant . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Oligodendrocytes , the myelin forming cells of the central nervous system , were cultured from newborn rat brain and optic nerve to allow us to analyze whether two transmembranous myelin proteins , myelin associated glycoprotein ( MAG ) and proteolipid protein ( PLP ) , were expressed together with myelin basic protein ( MBP ) in defined medium with low serum and in the absence of neurons . ^^^ We found that a proportion of oligodendrocytes derived from brain and optic nerve invariably express MBP , MAG , and PLP about a week after the emergence of GC , which occurs around birth . ^^^ In brain derived oligodendrocytes , MBP and MAG first emerge between the fifth and the seventh day after birth , followed by PLP 1 to 2 d later . ^^^ Each protein shows a specific cytoplasmic localization : diffuse for MBP , mostly perinuclear for MAG , and particulate for PLP . ^^^ MBP and PLP are only seen in these locations after 15 d . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Myelin associated glycoprotein ( MAG ) , 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNPase ) activity , myelin basic protein ( BP ) , and proteolipid protein ( PLP ) were quantitated in the brains of 20 day old Jimpy and control mice . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The 3 major protein components of CNS myelin are : myelin basic protein ( MBP ) , proteolipid ( PLP ) and 2 ' , 3 ' cyclic nucleotide phosphohydrolase ( CNP ) . ^^^ Previous work has shown that in jimpy animals the CNS contains reduced levels of MBP and CNP while PLP is undetectable . ^^^ In this study we have measured MBP , PLP and CNP in both the CNS ( cerebral hemispheres ) and PNS ( sciatic nerve ) of mice carrying the jimpy and shiverer mutations , individually and in combination . ^^^ This means that part of the biochemical phenotype of the jimpy mutation ( reduced levels of CNP and absence of PLP ) is suppressed by the shiverer mutation , and part of the biochemical phenotype of the shiverer mutation ( absence of MBP ) is suppressed by the jimpy mutation . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The predominant proteins in myelin are the major myelin proteolipid ( PLP ) and the myelin basic proteins ( MBP ) , which together account for 80 90 % of total myelin proteins . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Lymphocyte stimulation indices in active MS patients that were greater than 2 standard deviations above controls were as follows : 9 / 30 for MAG , 0 / 17 for PLP , and 8 / 81 for MBP . ^^^ No control subjects responded to MAG or PLP , and only 1 / 29 control subjects responded to MBP . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Myelin basic protein ( BP ) , proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) , and 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNPase ) activity were quantitated in the brains and spinal cords of normal and myelin deficient ( md ) rats at 8 , 12 , 18 , and 25 days of age . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
During brain development , the maximal period of PLP mRNA expression was from 14 to 25 days of age , and this was a similar time course to that for myelin basic protein mRNA expression . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
We have studied the sequence of expression in the oligodendrocyte of four myelin constituents : galactosylceramide ( GalC ) , myelin basic protein ( MBP ) , proteolipid protein ( PLP ) and Wolfgram protein ( W 1 ) . ^^^ Expression of PLP occurred shortly after that of MBP . ^^^ As judged both by the intensity of the labeling and the number of positive fibers , W 1 and MBP were the first antigens to be deposited , followed by PLP and finally by GalC . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
T cells were directly cloned from autopsied MS brain plaque tissue and reactivity was measured with the major encephalitogenic neuroantigens , myelin basic protein ( MBP ) , and proteolipid protein ( PLP ) . ^^^ None of 57 brain derived T cell clones proliferated to either MBP or PLP , although they responded well to PHA and IL 2 . ^^^ An additional 235 clones derived from the cerebrospinal fluid and 126 clones from the peripheral blood of other subjects with multiple sclerosis also did not proliferate to MBP or PLP . ^^^ These studies , the first to clone T cells directly from MS plaque tissue , suggest that the lack of consistent T cell reactivity to MBP or PLP in the peripheral blood of MS patients does not appear to be secondary to the sequestration of a large number of these cells in the brain . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Quantitative measurements with slot blot analyses showed that the levels of PLP and MBP RNAs peaked simultaneously at 18 days in nuclei but that maximal levels of PLP RNA lagged behind MBP RNA by several days in the polysomes . ^^^ Thus , the deletion of a portion of the MBP gene appeared to have a major effect on the expression of the PLP gene in this mutant . ^^^ In jimpy mice , where the mutation has been shown to involve the PLP gene , expression of MBP mRNA was also severely reduced , to less than 25 % of control values . ^^^ The extent to which the expression of PLP and MBP was affected by the quaking mutation depended on the age at which it was examined . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Mice primed in this manner exhibited significant Tprlf and DTH responses specific for both major myelin proteins , myelin basic protein ( MBP ) and proteolipid protein ( PLP ) . ^^^ A time course comparison between the induction of R EAE and the development of neuroantigen specific cell mediated immune ( CMI ) responses ( Tprlf and DTH ) revealed that the MBP and PLP specific Tprlf and DTH responses peaked prior to the onset of initial clinical symptoms and the DTH responses remained at significant levels throughout the relapsing course of the disease . ^^^ In vivo administration of a total of 100 micrograms anti L3T4 antibody , but not anti Lyt 2 antibody , resulted in delayed onset and reduced severity of clinical signs of R EAE concomitant with significantly reduced levels of MBP and PLP specific Tprlf and DTH responses . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Myelin associated glycoprotein ( MAG ) , myelin basic protein ( MBP ) , and proteolipid protein ( PLP ) were quantitated by immunoassays in nine plaque , inner periplaque , outer periplaque , and normal appearing white matter regions from brains of five multiple sclerosis patients and compared with the levels found in white matter samples of control subjects matched for age , postmortem time , and brain region . ^^^ In outer periplaque regions , MBP and PLP were close to control levels , but MAG was significantly reduced to a mean of 57 % of control . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Expression of PLP in the human spinal cord between 11 and 23 weeks after conception was examined and compared with the expression of the myelin basic protein ( MBP ) . ^^^ Immunoblot analysis of whole spinal cord homogenates indicated that polypeptides for MBP preceded the appearance polypeptides for PLP by 3 4 weeks . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Expression of the myelin basic protein ( MBP ) is also restricted to the oligodendrocytes in the central nervous system as is the PLP expression . ^^^ Homology search against the mouse MBP gene revealed that several boxes in the 5 ' flanking region of PLP show a high degree of homology with the sequence present in the MBP 5 ' flanking region , possibly of importance in the concomitant expression of both genes in the central nervous system . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
In the case of the dysmyelinating mutants shiverer and jimpy , the molecular defects involve the MBP gene and PLP gene , respectively ; most of the dysmyelinating mutants , including those in which the genetic defect is established , appear to exhibit pleiotropy with respect to the expression of other myelin protein genes . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Vibratome sections of brain were incubated with antisera to myelin basic protein ( MBP ) , myelin associated glycoprotein ( MAG ) , 2 ' : 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNP ) ( EC 3 . 1 . 4 . 37 ) , PLP , a synthetic PLP peptide , glial fibrillary acidic protein ( GFAP ) , and transferrin . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Endogenous zinc , as indicated by a dithizone binding method , eluted with fractions containing both MBP and proteolipid protein ( PLP ) . ^^^ Thus , one means whereby zinc stabilises association of MBP with brain myelin membranes may be by promoting its binding to PLP . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
We asked whether induction of specific immune tolerance to the major CNS myelin proteins , myelin basic protein ( MBP ) and proteolipid protein ( PLP ) , could inhibit the development of R EAE . ^^^ Sham tolerized controls developed significant MBP and PLP specific DTH responses before the onset of clinical R EAE . ^^^ In contrast , MSCH SP tolerized mice exhibited a dramatically reduced incidence of clinical and histologic signs of disease which correlated with the failure to develop MBP and PLP specific DTH responses . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The lymphocytes exhibited excellent proliferative responses to MBP , proteolipid apoprotein ( PLP ) and whole myelin , as well as to purified protein derivative ( PPD ) and concanavalin A ( ConA ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
We analyzed the location and abundance of transcripts for the 4 CNS myelin protein genes , myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) , and 2 ' , 3 ' cyclic nucleotide phosphohydrolase ( CNP ) , in the mouse cervical spinal cord from the time of rapid myelination until adulthood ( 8 45 d ) . ^^^ Total MBP and PLP mRNAs stayed high until 20 d and showed a minor decrease thereafter . ^^^ PLP , MAG , and CNP transcripts were clustered in the oligodendrocyte cell body , while MBP mRNAs were scattered throughout the cell body and processes . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Expression of mRNAs for the two major myelin proteins , myelin basic protein ( MBP ) and proteolipid protein ( PLP ) , was examined in a number of regions of the developing mouse brain using in situ hybridization . ^^^ In general , MBP and PLP mRNAs were observed to be coexpressed during ontogeny , prior to the histological appearance of myelin . ^^^ As myelination proceeded , two different labeling patterns were observed with the PLP and MBP 35S labeled cDNA probes . ^^^ These two distinctly different patterns of labeling are consistent with alternative intracellular trafficking of MBP and PLP mRNAs , in which PLP mRNAs remain associated with ribosomes within the cell soma and MBP mRNAs move from the cell soma to the oligodendrocyte processes at a specific stage early in myelinogenesis . ^^^ Additionally , the in situ hybridization studies revealed a population of unidentified cells residing in cortical molecular layers that express PLP mRNA in the absence of MBP mRNA . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Northern blots and slot blots of poly ( A ) + RNA from developing brain were sequentially probed with cDNAs specific for the oligodendrocyte markers glycerol phosphate dehydrogenase ( GPDH ) , myelin basic protein ( MBP ) , and proteolipid protein ( PLP ) , for the neuronal marker glutamic acid decarboxylase ( GAD ) , and for the astrocyte markers glial fibrillary acidic protein ( GFAP ) and glutamine synthetase ( GS ) . ^^^ The mRNA levels of the two myelin markers , MBP and PLP , were severely impaired in md rat brain during the entire myelinating period and represented less than 10 % of control mRNA levels at P 25 . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Developmental profile and differential localization of mRNAs of myelin proteins ( MBP and PLP ) in oligodendrocytes in the brain and in culture . ^^^ We applied the in situ hybridization technique to localize the mRNAs for two myelin proteins : proteolipid protein ( PLP ) and myelin basic protein ( MBP ) . ^^^ Expression of PLP and MBP genes progressed in the caudo cranial direction in the brain as far as we examined it in the tissue sections up to the 30th postnatal day : mRNAs of both genes were first detected in the pons and the medulla oblongata on the 3rd postnatal day and then in the cerebellum and the anterior part of the brain . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The major myelin proteins proteolipid protein ( PLP ) , DM 20 , and myelin basic protein ( MBP ) , which dominate the sodium dodecyl sulfate polyacrylamide gel electrophoresis pattern of myelin were minor components of the plasmalemma . ^^^ In order to analyze the minor proteins in myelin and compare them to the plasma membrane by two dimensional gel electrophoresis , myelin was extracted with chloroform : methanol to remove PLP , DM 20 , and MBP . ^^^ Even in the absence of PLP , DM 20 , and MBP the pattern of extracted myelin still differed from that of plasmalemma indicating that their minor protein compositions were not the same . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
At a concentration of 0 . 15 mM L cycloserine , the incorporation of [ 3H ] glycine into total proteins , proteolipid apoproteins ( APL ) , PLP , and myelin basic proteins ( MBP ) of the total homogenate was unaffected by the presence of the inhibitor , whereas the incorporation of [ 3H ] serine into glycosphingolipids decreased markedly . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
When tested for antigen specific proliferative activity , 26 TCLs responded to rubella antigen , 16 TCLs reacted to myelin basic protein ( MBP ) , four TCLs to proteolipid protein ( PLP ) , four to galactocerebrosides and two to actin . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Transport of PLP into myelin was compared to transport of MBP ; transport of both proteins was equally decreased as indicated by the similar ratio of labeled PLP to MBP in myelin from carriers compared to noncarriers . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The expression of myelin protein genes in Schwann cells has been studied in situ hybridization . 35S UTP labelled , antisense and sense RNA probes to the major protein Po , myelin basic protein ( MBP ) , myelin associated glycoprotein ( MAG ) and proteolipid protein ( PLP ) were employed with paraffin embedded sections , teased fibres and dissociated Schwann cells from sciatic nerves of rats . ^^^ The expression of MAG and PLP mRNA was markedly lower than Po and MBP . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Both myelin basic protein ( MBP ) and PLP message levels were reduced in MD rats to 10 20 % of the normal littermate controls , while the level of expression of an astrocyte specific gene , glial fibrillary acidic protein ( GFAP ) , was normal . ^^^ Although MBP and PLP mRNAs were equally depressed , only MBP was detected with immunolabeling of corpus callosum , while PLP was absent in oligodendrocytes both in vivo and in vitro . ^^^ In conclusion , the absence of PLP and reduced levels of MBP in the MD rats point to similarities with the jimpy mouse lesion . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Despite the absence of PLP , the major integral membrane protein of central nervous system ( CNS ) myelin , this study shows that there are many scattered myelinated fibers present in the spinal cord of this murine mutant which are immunocytochemically positive for myelin basic protein ( MBP ) , yet negative for PLP . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The LNC proliferated in vitro to PLP , but not to myelin basic protein ( MBP ) , and induced delayed type hypersensitivity . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The biochemical pathology was that of degradation of axonal ( neurofilaments ) and myelin proteins ( MBP and PLP ) with increased activities of proteolytic enzymes and particularly the neutral proteinase . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Immunocytochemical staining of adjacent sections following a variety of fixation methods showed that the myelinated fibers were positive for myelin basic protein but negative for proteolipid protein ( PLP ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The cellular and subcellular distributions of mRNAs encoding three myelin specific proteins myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , and Po protein were studied in tissue sections of developing rat nervous systems by in situ hybridization . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The line cells bore surface phenotypes of T helper / inducer cells ( Lyt 1+ , Lyt 2 , L3T4+ ) and responded well to bovine , rat , and guinea pig PLP but not to myelin basic protein . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Our results show that the relative amounts of mRNA for PLP and that for the major extrinsic protein of the myelin membrane , myelin basic protein , increase coordinately during the course of myelination in the brain . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Newly synthesized PLP and MBPs are not proteolytically processed , because the primary translation products synthesized in vitro had the same electrophoretic mobility and N terminal amino acid sequence as the mature PLP and MBP polypeptides . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Immunoblot analysis of antiserum to rat central nervous system ( CNS ) myelin revealed antibodies to myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , and numerous high molecular weight proteins . ^^^ Purified MBP and PLP were immunostained by anti CNS myelin sera and MBP and P 0 by anti PNS myelin sera , indicating that antigenic sites are preserved during protein purification . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Bands comigrating with myelin proteins , the basic protein ( MBP ) , the proteolipid protein ( PLP ) , and the Wolfgram protein ( WP ) doublet , were detected by Coomassie Blue staining and by autoradiography . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Cerebral cortical proteolipid protein ( PLP ) and myelin basic protein ( MBP ) mRNAs consistently exhibit approximately 200 % increases in IGF 1 Tg mice and approximately 50 % decreases in IGFBP 1 Tg mice . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
This gradient along the nerve of oligodendrocyte differentiation continues with oligodendrocytes near the chiasm expressing the genes and encoded proteins to MBP and PLP 3 d before oligodendrocytes near the eye . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Myelin sheaths of jprsh immunostain for myelin basic protein ( MBP ) and DM 20 , but very few contain PLP . ^^^ The majority of oligodendrocytes from mutant mice progress normally to express MBP ; approximately 30 % , relative to wild type , contain DM 20 at the in vivo age of 16 days , but very few immunostain for PLP or the O 10 and O 11 markers . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Although T cell responses to the quantitatively major myelin proteins , myelin basic protein ( MBP ) and proteolipid protein ( PLP ) , are likely to be of importance in the course of multiple sclerosis ( MS ) , cell mediated autoimmune responses to other myelin antigens , in particular quantitatively minor myelin antigens , such as myelin associated glycoprotein ( MAG ) and the central nervous system specific myelin oligodendrocyte glycoprotein ( MOG ) , could also play a prevalent role in disease initiation or progression . ^^^ Highly purified myelin antigens were used in this study to assess cell mediated immune response to MOG in MS patients , in the context of the reactivity to other myelin antigens , MBP , PLP , and MAG . ^^^ The incidence of responses to MBP , PLP , and MAG did not differ greatly between MS patients and control individuals . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
There are increased T and B cell responses to the myelin components myelin basic protein ( MBP ) and proteolipid protein ( PLP ) in both ON and MS , but there is little information on the types of cytokines produced by such cells . ^^^ Expression of these cytokines was studied in mononuclear cells from peripheral blood and cerebrospinal fluid ( CSF ) from patients with ON and MS after in vitro exposure to MBP and PLP , and in absence of antigen . ^^^ There were elevated levels of cells that in response to MBP and PLP expressed IFN gamma , IL 4 and TGF beta mRNA in blood and further enriched in CSF in both ON and MS , compared to patients with other neurological diseases . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
There are mononuclear cells in blood and cerebrospinal fluid of patients with MS that produce interferon gamma and interleukin 4 in response to myelin basic protein ( MBP ) and proteolipid protein ( PLP ) . ^^^ Blood and cerebrospinal fluid cells were exposed in short term cultures to MBP and PLP and , after hybridization with complementary DNA oligonucleotide probes , they were evaluated for TGF beta mRNA expression . ^^^ Patients with MS had higher numbers of MBP and PLP responsive TGF beta mRNA expressing cells in blood compared with control patients with other neurological diseases or myasthenia gravis and a five and threefold further increment in their cerebrospinal fluid . ^^^ Patients with other neurological diseases showed infrequent and low responses to MBP , PLP , and AChR . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Equal numbers of CD4+ T cells recognizing myelin basic protein ( MBP ) and proteolipid protein ( PLP ) are found in the circulation of normal individuals and multiple sclerosis ( MS ) patients . ^^^ While there were no differences in the frequencies of MBP and PLP reactive T cells after primary antigen stimulation , the frequency of MBP or PLP but not tetanus toxoid reactive T cells generated after primary recombinant interleukin ( rIL 2 ) stimulation was significantly higher in MS patients as compared with control individuals . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Human myelin basic protein ( hMBP ) and proteolipid protein ( PLP ) were used as antigens in a solid phase radioimmunoassay to determine relative frequencies of anti MBP and anti PLP in cerebrospinal fluid ( CSF ) of optic neuritis and multiple sclerosis ( MS ) patients . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Functional studies indicated that disruption of oligodendrocyte adhesion to a glial derived matrix by an RGD containing synthetic peptide resulted in a substantial decrease in the level of mRNAs for several myelin components including myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , and cyclic nucleotide phosphodiesterase ( CNP ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Myelin basic protein ( MBP ) and proteolipid protein ( PLP ) were purified from non MS human brain and used in solid phase radioimmunoassays to detect their specific antibodies in cerebrospinal fluid ( CSF ) of optic neuritis and clinically definite multiple sclerosis ( MS ) patients . ^^^ The remaining 6 patients with undetectable anti MBP had increased levels of anti PLP in their CSF . ^^^ Three of the 4 remaining patients with undetectable anti MBP had increased anti PLP in their CSF . ^^^ Of 110 MS patients with chronic progressive disease , 107 had increased CSF anti MBP and 2 had elevated anti PLP . ^^^ Of 87 MS patients in remission , 15 had modestly elevated anti MBP and none had detectable anti PLP . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
T cells recognizing the myelin components myelin basic protein ( MBP ) and proteolipid protein ( PLP ) are increased in multiple sclerosis ( MS ) , and there are elevated numbers of T cells recognizing the nicotinic acetylcholine receptor ( AChR ) in myasthenia gravis ( MG ) . ^^^ High numbers of IFN gamma and IL 4 mRNA expressing cells in response to MBP and PLP were detected in patients with untreated MS , and to AChR in MG . ^^^ The levels of IFN gamma and IL 4 mRNA positive cells in MS after culture in the presence of AChR , and in MG after culture in the presence of MBP or PLP , did not differ from those detected after culture without antigen . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
These results suggest that during the course of myelin breakdown caused by an immune response to one myelin component ( MBP ) , autoimmune reactivity to at least one additional myelin antigen ( PLP ) can arise . ^^^ Cells from 25 mice at different stages of experimental autoimmune encephalomyelitis ( EAE ) adoptively transferred using lymph node cells sensitized to a synthetic encephalitogenic peptide of myelin basic protein ( p 87 99 ) were examined for reactivity to a different encephalitogenic myelin antigen , proteolipid protein ( PLP ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Abnormal T cell responses to myelin components including myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , and myelin associated glycoprotein ( MAG ) have been implicated in the pathogenesis of multiple sclerosis . ^^^ Untreated patients with optic neuritis and multiple sclerosis had similarly raised levels of T cells recognising MBP , PLP , and MAG in peripheral blood . ^^^ The role of raised MBP , PLP , and MAG reactive Th 1 like cells found in optic neuritis and multiple sclerosis remains unexplained . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The elution was monitored of the two major protein components , i . e . myelin basic protein ( MBP ) and proteolipid protein ( PLP ) , as well as of minor components , viz . myelin associated glycoprotein ( MAG ) and myelin / oligodendrocyte glycoprotein ( MOG ) . ^^^ Whereas MBP and MOG elute as single sharp protein peaks upon chromatography , PLP and MAG are resolved into several different components . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
When OL cells are separated from human white matter and allowed to regenerate in vitro , a much larger proportion of these cells express developmentally regulated genes , while exon 2 MBP and proteolipid protein ( PLP ) transcripts characteristic of mature OL cells appear transiently downregulated . ^^^ Yet bFGF induces human oligodendrocytes to regenerate their processes rapidly in vitro and to express O 4 antigens as well as exon 2+ MBP , MyTI , and PLP transcripts . ^^^ In contrast , high levels of insulin in the absence of bFGF allow accumulation of exon 2 MBP and PLP transcripts in most OL cells at 2 3 weeks in vitro . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The histological findings were compared with those in acute EAE induced in the Lewis rat by inoculation with whole CNS tissue or with myelin basic protein : in PLP EAE , in contrast to these other models , the disease was essentially restricted to the CNS . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Thus , orally administered MBP or MBP peptides suppress proteolipid protein ( PLP ) induced EAE , whereas intravenously administered MBP does not . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Over expression of MBP and PLP messenger RNA in 8 day old trembler brain . ^^^ We measured a two to four fold increase for myelin basic protein ( MBP ) and proteolipid protein ( PLP ) mRNAS in the trembler samples . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
OBJECTIVE : To study the intrathecal synthesis of anti myelin basic protein ( MBP ) and anti proteolipid protein ( PLP ) antibodies in patients in the early stages of multiple sclerosis . ^^^ MAIN OUTCOME MEASURES : Numbers of anti MBP and anti PLP antibody secreting cells in peripheral blood and cerebrospinal fluid samples that were enumerated with an immunospot assay . ^^^ RESULTS : Cerebrospinal fluid cells that secreted anti MBP or anti PLP antibodies were detected in 10 of 15 and in 21 of 23 patients with acute ON , while they were detected in nine of 18 and in six of 18 patients with other neurological diseases , respectively . ^^^ CONCLUSIONS : These data suggest that anti PLP antibodies are a more specific finding in demyelinating disease than anti MBP antibodies . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Experimental autoimmune encephalomyelitis ( EAE ) is an inflammatory demyelinating disease of the central nervous system ( CNS ) and can be induced by inoculation of animals with homogenized CNS tissue or highly purified myelin proteins such as myelin basic protein ( MBP ) or proteolipid protein ( PLP ) . ^^^ We performed the present neurophysiological study to define the location of nerve conduction abnormalities in EAE induced by immunization with PLP ( PLP EAE ) and in EAE induced by immunization with MBP ( MBP EAE ) in the Lewis rat . ^^^ The distribution of nerve conduction abnormalities is consistent with previous histological studies showing that inflammation and primary demyelination are restricted to the CNS in PLP EAE , but are present in the CNS and in the spinal roots of the PNS in MBP EAE . ^^^ The restriction of functional abnormalities to the CNS in PLP EAE but not in MBP EAE may have implications for the human inflammatory demyelinating diseases , including multiple sclerosis . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Peptide sequences from human myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) , and myelin oligodendrocyte glycoprotein have been identified that bind to and form stable complexes with HLA A 2 . ^^^ MBP 110 118 , PLP 80 88 , MAG 287 295 , MAG 509 517 , and MAG 556 564 were all able to induce peptide specific HLA A 2 restricted CD8+ cytotoxic T lymphocyte ( CTL ) responses in vitro in HLA A2+ individuals . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Multiple sclerosis is assumed to be an autoimmune disease mediated by T cells specific for myelin protein , such as , myelin basic protein ( MBP ) and PLP . ^^^ To our surprise , the CDR 3 motifs of PLP specific clones resemble those of MBP specific clones . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The distinct characteristics of the PLP molecule that may contribute to complex immune responses to this protein are reviewed and these responses are compared with those to MBP , both in the pathology of EAE and at the level of the T cell . ^^^ Recent evidence demonstrating differences between T cell responses to PLP and MBP is reviewed . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
It is drawn largely from work published within the last ten years and discusses the immunology of PLP in the historical context of what has been learned from extensive studies on the immune response to myelin basic protein ( MBP ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
By contrast , the electron density profile calculated from a chemical model that includes lipids plus myelin basic protein ( but not PLP ) shows a higher density at the cytoplasmic than at the extracellular side . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Multiple sclerosis ( MS ) is associated with high levels of circulating T lymphocytes that respond to the myelin antigens myelin basic protein ( MBP ) and proteolipid protein ( PLP ) by producing various cytokines including interferon gamma ( IFN gamma ) that makes MS worse and transforming growth factor beta ( TGF beta ) , an endogenously produced immunosuppressant that might act beneficially . ^^^ Femtogram concentrations of rTGF beta 1 suppressed MBP , PLP and PPD induced upregulation of IFN gamma , IL 4 , IL 6 , tumor necrosis factor alpha ( TNF alpha ) , TNF beta and perforin in MS , and acetylcholine receptor ( AChR ) induced augmentation of these pro inflammatory cytokines in MG , but had no effects on autoantigen or PPD induced expression of IL 10 or TGF beta itself . rTGF beta 1 also suppressed numbers of myelin antigen reactive IFN gamma and IL 4 secreting cells in MS and AChR reactive IFN gamma and IL 4 secreting cells in MG . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Relative mRNA levels for myelin basic protein , proteolipid protein ( PLP ) , and 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase in lesions of IGF 1 treated rats were significantly higher than they were in placebo treated rats . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Although most studies have implicated myelin basic protein as a potent encephalitogenic myelin component , proteolipid protein ( PLP ) appears also to be a possible target antigen in the autoimmune response in MS . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Protein level immunofluorescence analysis by confocal microscopy reveals that in PLP minus mice there is a complete absence of PLP , a scattered appearance of MBP , and MAG expressed more widely in regions lacking MBP staining , which may be a compensatory mechanism . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
We compared the T cell responses of Lewis ( LEW ) and DA rats to guinea pig myelin basic protein ( MBP ) , the synthetic peptides corresponding to the epitopes that are encephalitogenic in the LEW strain ( MBP 73 86 , MBP 68 86 , and MBP 87 99 ) , and bovine proteolipid protein ( PLP ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
These same results are also observed in secondary oligodendrocyte cultures , although in general late myelin markers such as MBP and PLP are expressed at a much lower level in mouse cells than rat cells . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The T cell line , when tested for antigen specificity , reacted strongly with exon 2 peptide , but not with MBP peptides pAc 1 11 , p 43 88 , p 89 101 or PLP p 139 151 . ^^^ Splenocytes or lymphocytes isolated from mice at the second relapse were reactive with MBP p 43 88 , p 89 101 and PLP p 139 151 in addition to exon 2 peptide and MBP peptide Ac 1 11 . ^^^ T cell lines selected by culture with MBP exon 2 peptide or PLP p 139 151 from splenic cells from mice with relapsing EAE were weakly encephalitogenic ; however , T cell lines selected from the same mice with MBP pAc 1 11 were not encephalitogenic . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Transmembrane proteins like proteolipid protein ( PLP ) , the peripheral myelin protein 22 ( PMP 22 ) and P 0 as well as myelin basic protein ( MBP ) are crucial for this process . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
There was an increase in the amount of carbonyl groups ( CO ) , accompanied by an enhanced susceptibility to degradation by trypsin and subtilisin of myelin basic proteins ( MBP ) and of the major proteolipid protein ( PLP ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The genes expressed late in the myelination program ( PLP and MBP ) were affected more dramatically than those expressed earlier in oligodendrocyte development ( CNP and GPDH ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
One line ( N20 . 1 ) engineered from normal oligodendrocytes also expressed myelin basic protein ( MBP ) and proteolipid protein ( PLP ) mRNAs , genes normally expressed by mature , differentiated oligodendrocytes . ^^^ However , MBP and PLP polypeptides could not be detected by western blot or immunocytochemical staining at either the permissive or nonpermissive temperature . ^^^ Because the cell line expressed the MBP and PLP genes , it represents a `` mature ' ' oligodendrocyte , but the splicing patterns of these genes indicate that it is at an early stage of `` maturation . ' ' This cell line has now been passaged > 40 times with fidelity of phenotype and genotype . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Immunocytochemical colocalization of PKC with oligodendrocyte specific markers O 4 , galactosyl cerebroside , MBP , and PLP in enriched oligodendrocyte cultures suggested that the PKC enzyme activities assayed in these cultures were predominantly contributed by oligodendrocytes . ^^^ PKC inhibition resulting from long term exposure to 4 beta phorbol 12 , 13 dibutyrate ( 4 beta PDB ) reduced steady state levels of MBP , PLP , MAG , CNP , and PKC alpha mRNAs , as detected by slot blots or in situ hybridization , and downregulated the oligodendrocyte specific markers O 4 , galactosyl cerebroside , and the major constituent proteins MBP and PLP , as detected by immunocytochemistry . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Sublytic activation of serum C reduced accumulation of mRNA encoding proteolipid protein ( PLP ) and myelin basic protein ( MBP ) within 1 h , but not beta actin mRNA . ^^^ Experiments using C 7 deficient human serum to determine the role of TCC showed that selective MBP and PLP mRNA down regulation was achieved only when C 7 was reconstituted to form TCC . ^^^ Post transcriptional regulation was explored by determining mRNA decay , which demonstrated that the MBP and PLP mRNA were selectively destabilized when C 7 was reconstituted . ^^^ Limited exploration of the signals responsible for the TCC effect revealed that down regulation of mRNA by TCC was significantly influenced by Ca2+ on PLP , whereas MBP did not show the same Ca2+ sensitivity as PLP . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Northern blot analysis showed that the mRNA levels of PLP and myelin basic protein , two major myelin proteins produced by oligodendrocytes , were much reduced in the PMD brain , hence , there was a specific loss of oligodendrocytes . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The intensity of p75NGFR receptor like immunoreactivity and the mRNAs encoding p75NGFR , T alpha 1 alpha tubulin , GAP 43 and the myelin proteins MBP and PLP were measured in the developing cerebellum to study the effects of perinatal thyroid hormone imbalance in rats . ^^^ MBP and PLP mRNAs were first detected on P 2 of hyperthyroid rats , and they increased with age . ^^^ Hypo or hyperthyroidism did not affect the initial onset of MBP and PLP expression , however , hyperthyroidism increased levels of PLP and MBP mRNAs between P 2 and P 10 . ^^^ By contrast , the most consistent decrease in MBP and PLP mRNAs in rats with thyroid hormone deficiency was observed only on P 10 . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
All cytolytic PLP 40 60 TCL were CD4+ and MHC class 2 restricted and further analysis of MS 1 TCL showed that the PLP 40 60 TCL were restricted by DR 4 whereas the MBP TCL from MS 1 were restricted by DR 6 . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
MAG , myelin basic protein ( MBP ) , 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNP ) , proteolipid protein ( PLP ) and glial fibrillary acidic protein ( GFAP ) were quantified by western blot analysis in whole homogenates of spinal cords and hemispheres from affected goats at 1 , 3 and 6 days of age and from normal controls . ^^^ In mutant spinal cords , the deficits of MAG , CNP and PLP measured in whole homogenates corresponded reasonably well with the decreased myelin yields , but the levels of MBP were consistently much closer to control levels than those of the other myelin proteins . ^^^ A greater deficiency of PLP than MBP was also apparent in the myelin fractions purified from the affected spinal cords . ^^^ In homogenates of mutant hemispheres , MAG , MBP , PLP and CNP were undetectable or at trace levels in comparison to controls . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) , and 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNP ) were measured by densitometric scanning of western blots . ^^^ PLP and MBP increased from 10 30 % of normal in cerebellar white matter at birth to 40 60 % of normal at 6 months , suggesting some catch up in the amount of compact myelin with development . ^^^ A common finding in all regions examined was that MBP and PLP were reduced more than MAG and CNP . ^^^ This is probably explained by a greater deficit of compact myelin , in which MBP and PLP are localized , than of associated oligodendroglial membranes , in which MAG and CNP are concentrated . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ Myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) , and 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNP ) were measured by densitometric scanning of western blots . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
In contrast to the tumor parent cells , a hybrid clone M03 . 13 expressed surface immunoreactivity for galactosyl cerebroside and intracellular immunoreactivity for myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , and glial fibrillary acidic protein ( GFAP ) . ^^^ Consistent with immunohistochemical studies , northern blot analysis demonstrated that both MBP and PLP mRNA were up regulated in MO3 . 13 cells by PMA treatment . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Multiple sclerosis ( MS ) is characterized by perivascular inflammation and high levels of circulating T and B lymphocytes that respond to the myelin antigens myelin basic protein ( MBP ) and proteolipid protein ( PLP ) , thereby suggesting a role for immunoregulatory cytokines . ^^^ Cultures of MNC in presence and absence of antigen revealed that MBP and PLP induced strong responses in MS reflected by high levels of IFN gamma , IL 4 and TGF beta mRNA expressing cells . ^^^ Cytokine mRNA expressing cells were enriched in the MS patients ' cerebrospinal fluid , as were the cytokine mRNA positive cells detected after culture in presence of MBP and PLP , reflecting an autonomy of the immune response in this compartment . ^^^ Patients with non inflammatory neurological diseases and healthy subjects had either no or low numbers of IFN gamma , IL 4 and TGF beta mRNA expressing cells when blood MNC were examined without previous culture , and after culture in presence and absence of MBP , PLP and AChR as antigens . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Myelin basic protein ( MBP ) and proteolipid protein ( PLP ) , the most abundant proteins of central nervous system ( CNS ) myelin , have been extensively studied as possible primary target antigens in multiple sclerosis ( MS ) , a primary demyelinating autoimmune disease of the CNS . ^^^ We recently demonstrated a predominant response to MOG by peripheral blood lymphocytes from patients with MS tested for their reactivity against various myelin antigens , including MBP and PLP . ^^^ The disease presented with a delayed onset and with clinical signs which differed significantly in their progression and expression from the typical ascending paralysis of experimental autoimmune encephalomyelitis induced with other myelin components , such as MBP and PLP . ^^^ Myelin basic protein ( MBP ) and proteolipid protein ( PLP ) , the most abundant proteins of central nervous system ( CNS ) myelin , have been extensively studied as possible primary target antigens in multiple sclerosis ( MS ) , a primary demyelinating autoimmune disease of the CNS . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Moreover , the percentage of oligodendrocytes labelled with anti MBP antibodies decreased by 50 % , as well as that of oligodendrocytes expressing myelin oligodendrocyte glycoprotein ( MOG ) , whereas proteolipid protein ( PLP ) immunolabelled cells were less affected . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Biochemical sequences , as manifested by the appearance of the myelin associated lipids and myelin specific proteins , closely followed previously described anatomic sequences both temporally and by region , and were identical in all sites sampled : sphingomyelin was followed simultaneously by cerebrosides , MBP , PLP , and nonhydroxy sulfatide , followed by hydroxy sulfatide . ^^^ Cholesterol ester was transiently elevated during late gestation and early infancy , prior to and around the time of the appearance of cerebrosides , sulfatides , PLP , and MBP . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Taken together , the present results demonstrated that T cells , which are reactive to the myelin component , such as MBP or PLP , may be activated by HTLV 1 infection and infiltrate into the spinal cord lesions of HAM / TSP patients and suggested that an autoimmune mechanism may be involved in the pathogenesis of HAM / TSP . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
In addition , the abundant myelin antigens , myelin basic protein ( MBP ) and proteolipid protein ( PLP ) , are sequestered in the compact myelin . ^^^ To gain access to the large quantities of MBP and PLP hidden in the myelin sheath behind the blood brain barrier , autoreactive T lymphocytes must be activated from their resting , `` ignorant ' ' state . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Specific RNA probes were used to detect messenger RNA ( mRNA ) coding for proteolipid protein ( PLP ) , myelin basic protein ( MBP ) and glial fibrillary acidic protein ( GFAP ) . ^^^ PLP and MBP are markers for oligodendrocytes , GFAP for astrocytes . ^^^ Oligodendrocytes positive for PLP and MBP mRNA were found in both DBCC and brain sections of neonatal dogs . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Experimental allergic encephalomyelitis ( EAE ) is an autoimmune disease that can be induced in laboratory animals by immunization with the major myelin proteins , myelin basic protein ( MBP ) and proteolipid protein ( PLP ) . ^^^ When the TCR repertoire is limited by introduction of a novel rearranged TCR 5 beta 8 . 2 chain in transgenic SJL mice , EAE could be induced in the transgenic mice by immunization with the encephalitogenic epitopes of PLP , but not with the encephalitogenic epitope of MBP . ^^^ Thus , skewing the TCR repertoire affects the susceptibility to EAE by immunization with MBP but not with PLP . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
MBP deficiency corresponds approximately to the level of the hypomyelination , whereas PLP expression is drastically reduced . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Predominant protein components of myelin , myelin basic protein ( MBP ) and proteolipid protein ( PLP ) , have been considered as possibly relevant autoantigens , especially since both proteins are encephalitogenic in various laboratory animals . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
We have induced resistance to rat spinal cord homogenate ( RSCH ) induced experimental allergic encephalomyelitis ( EAE ) in SJL / J mice by vaccination with four T cell lines ( RZ 8 , RZ 15 , RZ 16 , and A 51 ) which were reactive to myelin basic protein ( MBP ) but not to proteolipid protein ( PLP ) . ^^^ We examined the effect of T cell vaccination on lymph node T cell proliferative responses to MBP , encephalitogenic peptides of PLP and MBP , OVA and anti CD 3 . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
To investigate whether the pathological changes in myelin , due to Pb exposure , might be partially mediated by modulations of the expression of genes involved in CNS myelin , we have examined the developmental profiles of the proteolipid protein ( PLP ) and myelin basic protein ( MBP ) , two major structural constituents of CNS myelin and 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNP ) , a non structural enzyme associated with myelin formation . ^^^ On PND 20 , Pb resulted in a dramatic stimulation of the mRNA levels of PLP and a small increase in MBP mRNA levels , but had no effect on the CNP message . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Because proteins can serve as excellent markers for the myelin formation process , we examined the developmental expression of several important myelin proteins ( PLP / DM 20 , MBP , CNP , MAG , and MOG ) in both pt mutant and control rabbit brain homogenates . ^^^ Expression of the investigated proteins occurs in rabbits as follows : CNP and MAG are already present at the early postnatal stage ; PLP / DM 20 and MBP appear about the 10th postnatal day ; MOG , expressed last , has been detected on the 28th postnatal day . ^^^ Whereas the MBP , CNP , MAG , and MOG content is only slightly reduced in mature pt mutant brain homogenates ( 80 90 % of control values ) , the amount of PLP corresponds to approximately 30 40 % of that present in controls . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
In our previous work , we presented developmental studies on the expression of several myelin specific proteins ( PLP / DM 20 , MBP , CNP , MAG , and MOG ) in the brain homogeates of both pt mutant and age matched control Chinchilla rabbits aged 1 120 postnatal days . ^^^ Whereas the content of CNP , MBP , and MAG in isolated myelin membrane fractions seemed close to normal , a drastic PLP deficiency was observed . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
It is surprising that the structure of CNS myelin should be almost normal in the absence of the major structural components , namely myelin basic protein ( MBP ) for shiverer of proteolipid protein ( PLP ) for jimpy . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
In comparison with disease induced by spinal cord homogenate or peptides of myelin oligodendrocyte glycoprotein ( MOG ) or proteolipid protein ( PLP ) , the low incidence indicates that , at least in ABH mice , MBP is a minor encephalitogen . ^^^ However , the data suggest the presence of a peptide core between MBP 21 26 ( HARHGF ) , which contains similar elements to the previously defined encephalitogenic MOG 1 22 and PLP 56 70 peptides . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Experimental autoimmune encephalomyelitis ( EAE ) , an animal model for multiple sclerosis , can be prevented by feeding myelin basic protein ( MBP ) or proteolipid protein ( PLP ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
We generated 4 , 620 T cell lines from 34 relapsing remitting MS patients ( 17 were fed bovine myelin daily ) , and each line was examined for proliferation to MBP , PLP , and TT and for secretion of IL 4 , IFN gamma , and TGF beta 1 . ^^^ The frequency of TGF beta 1 secreting T cell lines after MBP and PLP stimulation in fed patients was greater than that of nonfed patients . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Within 6 days in vitro they form extensive membranous sheets and acquire myelin associated glycoprotein ( MAG ) , proteolipid protein ( PLP ) , and myelin basic protein ( MBP ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Subcutaneously administered IGF 1 also increased relative mRNA levels of myelin basic protein ( MBP ) , proteolipid ( PLP ) and 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNP ) , thereby promoting myelin regeneration . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
However , the temporospatial relationship of expression of two other major myelin proteins , proteolipid protein ( PLP ) and myelin associated glycoprotein ( MAG ) to that of MBP during fetal development has not been assessed in human tissues . ^^^ By the late second trimester , expression of MBP , PLP and MAG was noted in all locations in the spinal white matter except for the corticospinal tract . ^^^ The results suggest that PLP and MAG are expressed later than MBP but follow similar spatial gradients . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Tg mice were immunized with peptides , myelin basic protein ( MBP ) 87 106 and proteolipid protein ( PLP ) 175 192 , that are considered to be immunodominant epitopes in HLA DR 4 individuals . ^^^ Non Tg mice immunized with either PLP 175 192 or MBP 87 106 did not develop EAE . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Here , we investigated whether in MS patients oral myelin treatment , containing both myelin basic protein ( MBP ) and proteolipid protein ( PLP ) , induced antigen specific MBP or PLP reactive T cells that either secreted IL 4 , TGF beta 1 , or alternatively did Th 1 type sensitization occur as measured by IFN gamma secretion . ^^^ Specifically , 4 , 860 short term T cell lines were generated to either MBP , PLP , or tetanus toxoid ( TT ) from 34 relapsing remitting MS patients : 17 orally treated with bovine myelin daily for a minimum of 2 yr as compared to 17 nontreated patients . ^^^ We found a marked increase in the relative frequencies of both MBP and PLP specific TGF beta 1 secreting T cell lines in the myelin treated MS patients as compared to non treated MS patients ( MBP P < 0 . 001 , PLP P < 0 . 003 ) . ^^^ In contrast , no change in the frequency of MBP or PLP specific IFN gamma or TT specific TGF beta 1 secreting T cells were observed . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Of the known Schwann cell proteins , MBP , P 2 , MAG , PLP , PO , PMP 22 and Connexin 32 , gene defects of the latter three have recently been shown to be responsible for some forms of hereditary sensory and motor neuropathies . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
These data show that when HSC 70 protein content is sufficiently reduced by antisense oligonucleotide , synthesis of MBP ( but not PLP or pi ) is correspondingly down regulated , and provide evidence consistent with the role of HSC 70 as a chaperone for MBP . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
In MS , T cell responses to MBP and proteolipid protein ( PLP ) have been studied and certain T cell immunodominant regions are suggested . ^^^ Before reaching this conclusion , we must show that the MBP or PLP specific polyreactive T cells are indeed encephalitogenic . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The cells , initially devoid of myelin basic protein ( MBP ) and PLP , co express these myelin proteins at approximately E16 . 5 / 17 . 0 . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The levels of mRNA for MAG , MBP and PLP in affected goat spinal cords compared with those of controls were equally decreased to approximately 50 % for the three myelin proteins . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
In patients with the demyelinating disease multiple sclerosis ( MS ) , multiple potentially autoantigenic epitopes have been identified on the two major proteins of the myelin sheath , myelin basic protein ( MBP ) and proteolipid protein ( PLP ) . ^^^ To generate a tolerogenic protein for the therapy of patients with MS , we have produced a protein fusion between the 21 . 5 kD isoform of MBP ( MBP21 . 5 ) and a genetically engineered form of PLP ( deltaPLP 4 ) . ^^^ Importantly , the administration of MP 4 completely suppressed the development of EAE initiated by the cotransfer of both MBP and PLP activated T cells . ^^^ Prevention of clinical disease after the intravenous injection of MP 4 was paralleled by the formation of long lived functional peptide MHC complexes in vivo , as well as by a significant reduction in both MBP and PLP specific T cell proliferative responses . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Whole mounts of optic nerve , chiasm , and optic tract were sectioned horizontally and incubated with antibodies to myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) , `` Rip , ' ' and the neurite inhibitory protein ( IN 1 ) , followed by visualization with diaminobenzidine and a peroxidase conjugated secondary antibody . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
As analyzed by indirect immunofluorescence , the A2B5 surface marker is absent , they express galactocerebroside and myelin specific proteins , such as myelin basic protein ( MBP ) , myelin associated glycoprotein ( MAG ) , proteolipidprotein ( PLP ) , and Wolfgram protein ( WP ) , but do not exhibit astrocytic properties , such as the expression of vimentin or the glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
In situ hybridization with probes specific for PLP , myelin basic protein , and MOG mRNA showed significantly higher numbers of positive cells in test animals compared with controls in all brain regions . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Furthermore , after 6 months of treatment , there was no increased sensitization to myelin antigens seen , as measured by antimyelin basic protein ( MBP ) or anti proteolipid apoprotein ( PLP ) antibodies . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
MBP peptide 89 101 and PLP peptide 178 191 induced EAE upon active immunization although proteolipid protein peptide 139 151 was ineffective in this regard . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Significant T cell proliferation to PLP 139 151 , but not to other PLP and myelin basic protein ( MBP ) epitopes , was observed in both males and females during the initial episode , recovery , and first relapse of clinical disease . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
In contrast to expectations , the peptide was found to augment not only EAE induced by MBP specific T cells , but also proteolipid protein ( PLP ) specific T cell or PLP peptide induced EAE in SJL / J mice , and MBP induced EAE and adjuvant arthritis ( AA ) in rats . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Demyelinating diseases , such as multiple sclerosis ( MS ) in man or experimental allergic encephalomyelitis ( EAE ) in rodents , may include an associated immune response directed against myelin protein antigens such as the proteolipid protein ( PLP ) and the myelin basic protein ( MBP ) . ^^^ Therefore , we wished to determine whether genes that encode the two major myelin protein components , PLP and MBP , were expressed in the human thymus . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Expression of the genes for the major CNS myelin proteins , PLP and the myelin basic protein , are most dramatically affected in the shaking pup , although reduced expression levels are observed for other oligodendrocyte specific genes such as 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase and glucose phosphate dehydrogenase . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
While a progressive increase in T cell responsiveness specific for the immunodominant MBP 87 106 region was observed during disease , there was no evidence of either intermolecular epitope spreading to the immunodominant region of proteolipid protein ( PLP ) 139 151 or of intramolecular epitope spreading to the exon 2 encoded region of MBP , which is spliced out of 18 . 5 kDa MBP . ^^^ In contrast during active EAE induced by MBP , epitope spreading to the immunodominant epitope of PLP , 139 151 , was observed . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Suramin , a polysulfonated napthylurea , decreased the proliferation of T cells , in the presence of various stimuli , such as guinea pig myelin basic protein ( MBP ) , mouse spinal cord homogenate ( MSCH ) , bovine proteolipid protein ( PLP ) , P 1 ( synthetic peptide 139 151 of PLP ) , and Mycobacterium tuberculosis ( MTB ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
In view of their ability to induce experimental autoimmune encephalomyelitis ( EAE ) , an animal model for MS , the quantitatively major malign proteins myelin basic protein ( MBP ) and proteolipid protein ( PLP ) have been extensively studied as the relevant primary antigens in MS , and therapeutic approaches have been targeted to counteract autoimmune reactivity to MBP and PLP . ^^^ Accordingly , copolymer 1 , a random synthetic amino acid copolymer crossreactive with MBP and highly protective against the induction of EAE with MBP or PLP , is not being extensively tested in clinical studies as a therapeutic agent for MS . ^^^ In this context , we have demonstrated that peripheral blood lymphocytes from patients with MS respond predominantly to myelin oligodendrocyte glycoprotein ( MOG ) rather than to MBP or PLP , suggesting an important role for cell reactivity against MOG in the pathogenesis of MS . ^^^ Therefore , notwithstanding the importance of the autoimmune reactivity to MBP and PLP in MS , the potentially pathogenic autoimmune reactivity to MOG must now also be taken into consideration in therapeutic approaches to MS . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
With increasing postnatal ages , cells representing maturing oligodendrocytes which co label for PLP and MBP are present in the optic pathways . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Experimental allergic encephalomyelitis ( EAE ) is inducible in experimental animals immunized with myelin basic protein ( MBP ) , proteolipid protein ( PLP ) or their peptides . ^^^ We compared T cell responses to encephalitogenic epitopes of PLP ( 43 64 ) and MBP ( Ac 1 11 ) in a single mouse strain , ( PL / J 10 SJL ) F 1 . ^^^ MBP ( 1 11 ) specific T cell hybridomas expressed predominantly TCR 5 beta 8 or 5 beta 4 , while PLP ( 43 64 ) specific hybridomas expressed a diverse TCR repertoire . ^^^ Mice injected with SEB and immunized with MBP ( Ac 1 11 ) showed significant inhibition of EAE , whereas SEB pretreated mice immunized with PLP ( 43 64 ) had an increased severity of EAE and developed a chronic disease . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The examination of an experimental animal model for MS , experimental allergic encephalomyelitis ( EAE ) , has demonstrated that myelin basic protein ( MBP ) or proteolipid protein ( PLP ) specific T cells mediate the destruction of CNS myelin . ^^^ In recent years , elegant studies in EAE have shown that encephalitogenic T cells recognize short peptides of MBP or PLP in the context of MHC / HLA class 2 molecules , express a restricted number of T cell receptor ( TCR ) molecules and secrete interferon gamma and tumor necrosis factor alpha / beta . ^^^ Although MBP and PLP specific T cells with properties similar to those that are encephalitogenic in animals can be isolated from patients , they are not specific for MS and occur with similar frequency in controls . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
For instance , it was demonstrated that activated myelin basic protein ( MBP ) and proteolipid protein ( PLP ) specific T cells accumulate in the central nervous system ( CNS ) , and that clonally expanded MBP specific T cells persist for several years in the blood of patients with MS . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
In addition , Gtx binds to several sites within the MBP , PLP and Gtx promoters in a sequence specific manner probably by way of the core homeodomain binding motif . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Using in situ hybridization of cultured rat OLG we found that mRNAs are localized to at least three different locations : 1 ) to the perinuclear region [ myelin associated glycoprotein ( MAG ) mRNA ] ; 2 ) mainly to the processes ( the mRNA for the 14 kDa isoform of MBP ) ; and 3 ) to both the perinuclear region and the primary processes [ 2 ' , 3 ' cyclic nucleotide phosphodiesterase ( CNPase ) and proteolipid protein ( PLP ) mRNAs ] . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
To assess the role of thyroid hormone on myelin gene expression , we have studied the effect of hypothyroidism on the mRNA steady state levels for the major myelin protein genes : myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) and 2 ' : 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNP ) in different rat brain regions , during the first postnatal month . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Autoaggressive T cells specific for myelin proteins like proteolipid protein ( PLP ) and myelin basic protein ( MBP ) are thought to play a major role in the pathogenesis of demyelinating diseases of the central nervous system ( CNS ) . 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNPase ) is the third most abundant myelin protein in the CNS . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The cellular / regional expression of myelin specific proteins : PLP , MBP , CNP ase , MAG and MOG was investigated in the brains of 14 and 42 days old control and pt mutant rabbits . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Functional analysis in vivo of the double mutant mouse deficient in both proteolipid protein ( PLP ) and myelin basic protein ( MBP ) in the central nervous system . ^^^ To unravel the functions of the two dominant myelin proteins in the CNS , proteolipid protein ( PLP ) and myelin basic protein ( MBP ) , we generated and characterized the homozygous double mutant mouse line ( plp / , mbp / ) , which is viable and fertile . ^^^ Plasma membrane processes of oligodendrocytes deficient in PLP and MBP , but not in myelin associated glycoprotein ( MAG ) , spirally wrap large diameter axons , tightly adhering at their extracytosolic surfaces and forming a pseudo compacted myelin . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Gtx mRNA accumulates in parallel with the RNAs encoding the major structural proteins of myelin , myelin basic protein ( MBP ) , and proteolipid protein ( PLP ) during postnatal brain development ; Gtx mRNA decreases in parallel with MBP and PLP mRNAs in the brains of myelin deficient rats , which have a point mutation in the PLP gene . ^^^ These data thus demonstrate that Gtx is expressed uniquely in differentiated oligodendrocytes in postnatal rodent brain and that its expression is regulated in parallel with the major myelin protein mRNAs , encoding MBP and PLP , under a variety of physiologically relevant circumstances . ^^^ Immunoprecipitation of labeled DNA fragments encoding either the MBP or PLP promoter regions with this fusion protein has identified several Gtx binding fragments , and we have confirmed these data using an electrophoretic mobility shift assay . ^^^ In this way we have identified four Gtx binding sites within the first 750 bp of the MBP promoter and four Gtx binding sites within the first 1 . 3 kb of the PLP promoter . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Furthermore , preimmunization with L144 / R147 protected mice from EAE induced with the encephalitogenic peptides PLP ( 178 191 ) and myelin oligodendrocyte protein ( MOG ) residues 92 106 and with mouse myelin basic protein ( MBP ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The examination of an experimental animal model for MS , experimental allergic encephalomyelitis ( EAE ) , demonstrated that myelin basic protein ( MBP ) or proteolipidprotein ( PLP ) specific T cells mediate the destruction of CNS myelin . ^^^ In recent years , elegant studies in EAE showed that encephalitogenic T cells recognize short peptides of MBP or PLP in the context of MHC / HLA class 2 molecules , express a restricted number of T cell receptor ( TCR ) molecules and secrete interferon gamma and tumor necrosis factor alpha / beta . ^^^ MBP and PLP specific T cells with similar properties could also be isolated from MS patients and control individuals . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Northern blot analyses showed a rapid reduction of MBP and PLP gene expression induced by mezerein , and both morphological and biochemical findings indicate a drastic loss of compact myelin . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The most abundant mmsv ( s ) in the adult CNS are present at detectable levels after expression of the myelin basic protein ( Mbp ) gene and marginally after or coincident with the proteolipid protein ( Plp ) gene . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Myelin basic protein ( MBP ) and proteolipid protein ( PLP ) were assayed in cerebrum by immunoblots and Northern blot . ^^^ One week after induction of hydrocephalus , CGa1T and GPCP activity were reduced in the corpus callosum there was less MBP and PLP in the cerebrum , and myelin sheaths around axons greater than 0 . 4 micron in diameter were abnormally thin . ^^^ Myelin basic protein ( MBP ) and proteolipid protein ( PLP ) were assayed in cerebrum by immunoblots and Northern blot . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
A direct comparison of MBP and proteolipid protein ( PLP ) gene expression with that of MOBP by in situ hybridization revealed a very similar regional distribution . ^^^ In contrast to MBP and PLP , expression of MOBP was undetectable in the peripheral nervous system during the entire development . ^^^ MOBP showed a very specific timing of expression : in spinal cord and brain , MOBP gene expression occurred significantly later ( 2 3 days ) than that of MBP and PLP , but slightly earlier than myelin oligodendrocyte glycoprotein gene expression . ^^^ MOBP proteins appeared in spinal cord and brain stem also after MBP protein , suggesting that the MOBPs functionally act after the structural myelin proteins MBP and PLP . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Interestingly , the ratio of PLP mRNA stained cells : myelin basic protein ( MBP ) mRNA stained cells in normal white matter and low grade astrocytoma was about 2 : 1 but approached 1 : 1 with increasing tumor grade . ^^^ Unlike astrocytomas , oligodendrogliomas were found consistently to contain few PLP mRNA or MBP mRNA expressing cells . ^^^ In contrast , gemistocytic astrocytomas , typically highly invasive tumors , contained high numbers of PLP positive cells and a ratio of PLP mRNA : MBP mRNA stained cells of about 1 . 5 : 1 , similar to low grade astrocytomas . ^^^ For example , gemistocytic astrocytes , which were found to be strongly vimentin mRNA positive , contained little glial fibrillary acidic protein mRNA and did not stain for PLP or MBP mRNAs . ^^^ In addition , the expression of mRNAs for the two major myelin protein genes , PLP and MBP , could be used to predict the grade and extent of tumor infiltration in astrocytomas . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Immunohistochemical staining methods and antibodies against bovine proteolipid protein ( PLP ) , synthetic tridecapeptide of bovine PLP , human myelin basic protein ( MBP ) , human myelin associated glycoprotein ( MAG ) ; and 2 . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Here we demonstrate heterogenous T cell responses to PLP 40 60 , PLP 89 106 and MBP 87 106 in both MS patients and controls . ^^^ PLP 40 60 specific cytolytic TCL were increased in MS patients , whereas healthy controls had increased percentages of cytolytic TCL responding to PLP 89 106 and MBP 87 106 . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Expression levels of mRNAs coding for the myelin proteins PLP and MBP were substantially reduced in the hippocampus and cortex at P 20 , but not in the sciatic nerve . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
A computer algorithm was used to examine the sequences of the two major encephalitogenic proteins of myelin , MBP and PLP , for HLA A 2 binding motifs . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
In situ expression of PLP / DM 20 , MBP , and CNP during embryonic and postnatal development of the jimpy mutant and of transgenic mice overexpressing PLP . ^^^ We analyzed by in situ hybridization the spatiotemporal expression of dm 20 , myelin basic protein ( MBP ) and 2 ' 3 ' cyclic nucleotide phosphodiesterase ( CNP ) during embryonic and postnatal development of the normal mouse and two plp / dm 20 mutants : the jimpy mouse and a transgenic mouse overexpressing the plp gene . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Disease in these rEAE models was induced by the adoptive transfer of T lymphocytes specific for 18 . 5 kDa MBP , MBP peptide 83 102 , or PLP peptide 139 151 in the SJL / J mouse and the adoptive transfer of T lymphocytes specific for MBP peptide Ac 1 9 in the ( SJL / J 10 PL / J ) F 1 mouse . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Compared to the expression of myelin basic protein ( MBP ) , another major myelin protein in the central nervous system , the developmental changes of PLP is in the same order as MBP , but the PLP immunoreactivity revealed greater and earlier appearance in the cerebrum than that of MBP in the fetal period . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
IL 2 , INF gamma secreting cells before MP in relapsing MS patients and those in remission significantly increased in response to myelin basic protein ( MBP ) 4 14 , 68 84 and proteolipid protein ( PLP ) . ^^^ INF gamma secreting cells after MP therapy in relapsing MS patients significantly increased in response to MBP 4 14 , 68 84 , and PLP . ^^^ IL 2R secreting cells in MS patients in remission significantly increased in response to MBP 4 14 , 68 84 , and PLP and were significantly higher after MP therapy in relapsing MS patients and in controls . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
In mice , EAE is induced by immunizing with spinal cord homogenate , components of the myelin sheath , such as myelin basic protein ( MBP ) or proteolipid protein ( PLP ) , or peptides derived from these components . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Two such genes are the human myelin basic protein ( MBP ) and proteolipid protein ( PLP ) genes , whose products make up approximately 80 % of the total protein in CNS myelin . ^^^ Here we test the hypothesis that mutations in the human MBP and PLP genes might be associated with MS by examining the entire expressed sequence of both genes by single strand conformation polymorphism ( SSCP ) analysis , using a panel of 71 MS patients and 71 controls . ^^^ Three base changes were found in the human PLP gene and nine base changes in the human MBP gene ; these were essentially equally distributed between patients and controls . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Levels of message for UDP galactose : ceramide galactosyl transferase ( CGT ) , a key enzyme in the synthesis of GalC and sulfatide , were compared with those of MBP and proteolipid protein ( PLP ) , before and after elevation of cAMP . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
In this study , we demonstrate that soluble products of these PLP specific CD8+ CTL can chemoattract CD4+ T cells that are specific for a myelin basic protein peptide and a PLP peptide , and that the majority of this chemotactic activity is mediated by IFN inducible protein 10 . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
These included epitopes Ac 1 9 and 89 101 of myelin basic protein ( MBP ) and 139 151 from proteolipid protein ( PLP ) . ^^^ By contrast , PLP [ 139 151 ] was able to induce bystander suppression of T cells responsive to both itself and the two epitopes from MBP . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Affected transgenic mice of various strains had severely hypomyelinated central nervous systems and expressed low levels of c myc , myelin basic protein ( MBP ) and proteolipid protein ( PLP ) mRNAs in the brain . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Similar swellings were absent in dysmyelinated shiverer mice , which lack myelin basic protein ( MBP ) , but recurred in MBP * PLP double mutants . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
In this paper we investigated , in patients with MS , whether oral myelin treatment ( myelin containing both MBP and PLP ) induced antigen specific MBP or PLP reactive T cells that were either Th 2 like ( secreted IL 4 or TGF beta 1 ) , or alternatively whether Th 1 type sensitization occurred as measured by IFN gamma secretion . ^^^ Specifically , 4 , 860 short term T cell lines were generated to either MBP , PLP or TT from 34 relapsing remitting patients with MS ; 17 were orally treated with bovine myelin daily for a minimum of two years as compared to 17 non treated patients . ^^^ We found a marked increase in the relative frequencies of both MBP and PLP specific TGF beta 1 secreting T cell lines in the myelin treated MS patients as compared to non treated MS patients ( MBP , p < 0 . 001 ; PLP , p < 0 . 003 ) . ^^^ In contrast , no changes in the frequency of MBP or PLP specific IFN gamma or TT specific TGF beta 1 secreting T cells were observed . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The genes include thymidine kinase ( TK ) , p 53 tumor suppressor , c fos , renin , myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , transferrin , transferrin receptor , platelet derived growth factor A ( PDGF A ) , platelet derived growth factor B ( PDGF B ) , platelet derived growth factor receptor alpha ( PDGF alpha receptor ) , platelet derived growth factor receptor beta ( PDGF beta receptor ) , glial fibrillary acidic protein ( GFAP ) , transforming growth factor beta 1 ( TGF beta 1 ) , basic fibroblast growth factor ( bFGF ) , fibroblast growth factor receptor 1 ( FGF R 1 ) , insulin like growth factor 1 ( IGF 1 ) , and somatostatin . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The maturation of these newly formed oligodendrocytes results in the upregulation of expression of a variety of myelin specific genes such as MBP and PLP and the subsequent elaboration of the myelin organelle . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
To investigate whether the increased IL 4 secretion was myelin antigen specific , we generated 3990 short term T cell lines to myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , or tetanus toxoid ( TT ) from 31 progressive MS patients : 11 MS patients treated with CY / MP , 10 MS patients treated with MP alone , and 10 untreated MS patients . ^^^ We found increased frequencies of both MBP and PLP specific IL 4 secreting T cell lines in CY / MP treated patients compared to untreated MS patients . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The appearance of immunoreactive fibers for PLP was usually earlier than that for myelin basic protein ( MBP ) and the pattern of difference between PLP and MBP can be classified into three groups : ( 1 ) their time of appearance and progress are almost the same , as in the optic tract ; ( 2 ) the appearance and progress of PLP occurs earlier than those of MBP , as in the pyramidal tract ; ( 3 ) the appearance of PLP occurs earlier than that of MBP , but their progress is the same . ^^^ Our findings revealed that the time of appearance and progress of myelination as measured by PLP are different among the nervous pathways , and that there is also a difference between PLP and MBP . ^^^ This difference between PLP and MBP may indicate a functional difference between them . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
MBP , anti MBP and anti PLP antibodies , and intrathecal complement activation in multiple sclerosis . ^^^ We compared total intrathecal synthesis of IgA , IgG , and IgM , the number of cells secreting anti myelin basic protein ( MBP ) and anti proteolipid protein ( PLP ) antibodies of the IgG isotype and intrathecal activation of the complement cascade in patients with possible onset symptoms of MS ( n = 18 ) or clinically definite MS ( n = 30 ) . ^^^ Intrathecal IgG , IgA and IgM synthesis also correlated weakly with the presence of cells secreting anti MBP or anti PLP autoantibodies . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The genes include thymidine kinase ( TK ) , c fos , renin , myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , glial fibrillary acidic protein ( GFAP ) , insulin like growth factor 1 ( IGF 1 ) , and somatostatin . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Concomitantly , as investigated by RT PCR analysis , the transcriptional activity of c fos and c jun was stimulated , without altering mRNA expression of the myelin specific genes MBP , MAG , and PLP . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Until recently , the search for the ' culprit ' autoantigen towards which deleterious autoimmunity is directed in multiple sclerosis ( MS ) centered mostly on myelin basic protein ( MBP ) and proteolipid ( PLP ) , the two most abundant protein components of central nervous system ( CNS ) myelin , the target tissue for the autoimmune attack in MS . ^^^ Although such research has yielded important data , furthering our understanding of the disease and opening avenues for possible immune specific therapeutic approaches , attempts to unequivocally associate MS with MBP or PLP as primary target antigens in the disease have not been successful . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
We evaluated expression of mRNA for myelin proteins using in situ hybridization with oligonucleotide probes to proteolipid protein ( PLP ) , the major protein in central myelin ; myelin basic protein ( MBP ) , a major component of central myelin and a minor component of peripheral myelin ; and protein zero ( P 0 ) , the major structural protein of peripheral myelin , as well as myelin transcription factor 1 ( MYT 1 ) . ^^^ We found reduced expression of PLP and MBP chronically after SCI in the dorsal , lateral , and ventral white matter both rostral and caudal to the injury epicenter . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Both myelin basic protein ( MBP ) and proteolipid protein ( PLP ) are expressed at the RNA and protein level in lymph node , thymus and spleen of SJL mice with relapsing EAE . ^^^ Further , T lymphocyte lines from SJL mice specific for the immunodominant and subdominant epitopes of MBP and PLP can recognize endogenous protein within cells derived from lymphoid tissues . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Oligodendrocytes , the presumed cell of origin of oligodendrogliomas , highly express the genes encoding myelin basic protein ( MBP ) and proteolipid protein ( PLP ) . ^^^ MBP and PLP were highly expressed in all oligodendrogliomas and minimally expressed in glioblastomas multiforme . ^^^ MBP was highly expressed in mixed oligoastrocytomas , whereas PLP expression was minimal . ^^^ The association between tumor classification and expression of the MBP and PLP genes was statistically significant . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
To estimate the Ag specificities of dominant clonotypes in the activated T cells , these samples were examined in parallel with Th 1 T cell clones specific for myelin basic protein or proteolipid protein ( PLP ) derived from the same patients . ^^^ Analysis of two patients demonstrated that the dominant clonotypes would contain those specific for myelin basic protein or PLP . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
We have cloned and overexpressed the PLP 1 domain in Escherichia coli by using a T 7 RNA polymerase promoter system or as a maltose binding protein ( MBP ) fusion protein . ^^^ In contrast to the cleavage activity demonstrated for PLP 1 , no in vitro cleavage in cis or in trans could be detected with PLP 2 expressed either as a polypeptide , including flanking viral sequences , or as an MBP fusion enzyme . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Some were positive for myelin basic protein , proteolipid protein ( PLP ) , and myelin oligodendrocyte glycoprotein , but the majority were positive for human 2 ' 3 ' cyclic nucleotide 3 ' phosphodiesterase and all were positive for carbonic anhydrase 2 , confirming that they are oligodendrocytes . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
PLP rats were more sensitive to experimental autoimmune encephalomyelitis mediated by T cells specific for PLP , but not another encephalitogenic myelin protein , MBP . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
To analyze TNF alpha and IGF 1 actions on oligodendrocyte function , we measured the abundance of messenger RNAs ( mRNAs ) for two major myelin specific proteins , myelin basic protein ( MBP ) and proteolipid protein ( PLP ) . ^^^ While TNF alpha decreased MBP and PLP mRNA abundance by 5 to 6 fold , IGF 1 abrogated TNF alpha induced reductions in a dose and time dependent manner . ^^^ The changes in MBP and PLP mRNA abundance could not be completely explained by the changes in oligodendrocyte number , indicating that myelin protein gene expression is regulated by both TNF alpha and IGF 1 . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
To gain insights into the mechanisms of myelin repair in the CNS and to establish the extent to which this process resembles myelination in development we have examined the patterns of expression of transcripts of the major myelin proteins , myelin basic protein ( MBP ) and proteolipid protein ( PLP ) during remyelination of lysolecithin induced demyelination in the adult rat spinal cord . ^^^ Injection of 1 microliter 1 % lysolecithin into the dorsal funiculus caused a dramatic decrease in levels of MBP exon 1 and MBP exon 2 containing transcripts and PLP / DM20 transcripts . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The N20 . 1 murine OL cell line provides a model of an intermediate stage of OL maturation in which to study responses to Ca ( 1 ) increases with regard to viability , as well as the expression of mRNAs for myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , DM 20 , SCIP , and the immediate early genes ZIF 268 , c fos , and c jun . ^^^ With both agents , SCIP , MBP , and PLP mRNA levels were unaffected by 3 hr , but markedly reduced after 18 hours . ^^^ Thus , in N20 . 1 cells , increased calcium affects the IEG c jun first , SCIP is coordinately decreased with MBP and PLP mRNAs at a later time point , and DM 20 message is under different regulation than PLP . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
We compared T cell proliferative responses in peripheral blood following challenge in vitro with myelin / oligodendrocyte glycoprotein ( recombinant protein , rMOG ) , myelin basic protein ( MBP ) and proteolipid apoprotein ( PLP ) in 50 patients with MS and 40 healthy controls . ^^^ By contrast , reactivity against PLP was present in only one ( 2 % ) MS patient and six ( 15 % ) controls , and no reactivity against MBP was found in any subject . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Our data show that the expression of myelin basic protein ( MBP ) and proteolipid protein ( PLP ) of the little brain exhibit the same temporal pattern and amount as that of the wild type brain . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
We performed linkage analysis of MS to markers in chromosomal regions that include the genes encoding myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) , oligodendrocyte myelin glycoprotein ( OMGP ) , and myelin oligodendrocyte glycoprotein ( MOG ) in a well characterized population of 65 multiplex MS families consisting of 399 total individuals , 169 affected with MS and 102 affected sibpairs . ^^^ Results indicate that MAG , MBP , OMGP , and PLP genes do not have a significant genetic effect on susceptibility to MS in this population . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Monoclonal antibodies directed to myelin basic protein ( MBP ) clones 1 , 12 , 17 , 22 , 26 , proteolipid protein ( PLP ) , galactocerebroside ( GalC ) and myelin oligodendrocyte glycoprotein ( MOG ) clones Y 1 , Y 4 , Y 6 , Y 7 , Y 9 , Y 10 , Y 11 and Z 12 were incubated with purified murine myelin labeled with DiI . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Myelin destruction during the initial relapsing phase of R EAE in SJL mice initiated by immunization with the proteolipid protein ( PLP ) epitope PLP 139 151 is associated with activation of T cells specific for the endogenous , non cross reactive PLP 178 191 epitope ( intramolecular epitope spreading ) , while relapses in R EAE induced with the myelin basic protein ( MBP ) epitope MBP 84 104 are associated with PLP 139 151 specific responses ( intermolecular epitope spreading ) . ^^^ Collectively , these data indicate that epitope spreading is B 7 1 dependent , plays a major pathologic role in disease progression , and follows a hierarchical order associated with the relative encephalitogenic dominance of the myelin epitopes ( PLP 139 151 > PLP 178 191 > MBP 84 104 ) . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Others have indicated that myelin composition may also vary with respect to myelin basic protein ( MBP ) and proteolipid protein ( PLP ) , and the small ( S ) and large ( L ) isoforms of myelin associated glycoprotein ( MAG ) . ^^^ In this study , we have determined the expression of myelin proteins in oligodendrocyte unit phenotypes 1 4 , which myelinate fibres ranging in diameter from 0 . 3 12 microns diameter in the AMV , by using double immunolabelling for Rip , which labels entire units , and MBP , PLP , myelin oligodendrocyte glycoprotein ( MOG ) , L MAG and S MAG . ^^^ All units , irrespective of fibre diameter , expressed Rip , MBP , PLP and MOG . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
A decade ago the myelin basic protein ( MBP ) and proteolipid protein ( PLP ) genes were thought to be expressed solely in myelin forming cells , and their products were thought to be structural components of myelin . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The expression of the late myelin proteins MBP and PLP in oligodendrocytes was impeded by both PMA and FGF 2 treatment , and , as a consequence , also myelin formation . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Results show that lymphocytes stimulated with the major PLP epitope have a powerful capacity for demyelinating these cultures , while MBP stimulated lymphocytes and lymphocytes from control animals do not . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The transduced MBP specific Th 1 cells were found to lose the capacity to provoke EAE in BALB / c mice , and to gain instead the ability to protect against EAE in ( SJLxBALB / c ) F 1 mice immunized with proteolipid protein ( PLP ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Although all tested proteins could be floated to a low density fraction , proteolipid protein ( PLP ) , myelin basic protein ( MBP ) and myelin associated glycoprotein ( MAG ) were solubilized by the detergent extraction , and connexin 32 ( Cx 32 ) and oligodendrocyte specific protein ( OSP ) met only some of the criteria for DIGCEMs . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
In contrast , no significant differences in the gross morphology and innervation of the organ of Corti or myelin gene expression in the auditory system , detected as myelin basic protein ( MBP ) and proteolipid protein ( PLP ) mRNA using Northern blot approach , were observed when TH supply was delayed for few days . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Consistent with this hypothesis , Schwann cells in adult P 0 / mice display a novel molecular phenotype : PMP 22 expression is down regulated , MAG and PLP expression are up regulated , and MBP expression is unchanged . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Early onset of axonal degeneration in double ( plp / mag / ) and hypomyelinosis in triple ( plp / mbp / mag / ) mutant mice . ^^^ Double ( plp / mag / ) and triple ( plp / mbp / mag / ) null allelic mouse lines deficient in proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) , and myelin basic protein ( MBP ) were generated and characterized genetically , biochemically , and morphologically including their behavioral capacities . ^^^ CNS axons of the plp / mbp / mag / mouse are hypomyelinated and ensheathed by `` pseudomyelin ' ' with disturbed protein and complex lipid composition . ^^^ The shiverer trait in the plp / mbp / mag / similar to the plp / mbp / mutant is significantly ameliorated , and its lifespan is considerably prolonged . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The effects of FGF 9 and FGF 2 on FGFR and myelin protein levels were comparable ; both up regulated FGFR 1 , and down regulated FGFR 2 , CNP , PLP and MBP . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Cotransfection of a Gtx expression vector into a variety of cell lines , including oligodendrocytes , along with constructs containing portions of the PLP , MBP , or Gtx promoters fused to a reporter gene , however , did not modulate transcription from any of these promoter constructs . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
We performed monthly limiting dilution assays for 12 16 mo in four healthy subjects and five patients with relapsing remitting MS to quantify the frequencies of circulating T cells proliferating in response to PLP ( 41 58 ) , PLP ( 184 199 ) , PLP ( 190 209 ) , myelin basic protein ( MBP ) , MBP ( 82 100 ) , and tetanus toxoid . ^^^ Compared with healthy controls , MS patients had significantly more frequent surges of T cells reactive to the 184 209 region of PLP , but infrequent surges of T cell reactivity to MBP ( 82 100 ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Immunohistochemical analysis of taiep brains and spinal cords revealed a gradual decrease in levels of several myelin proteins including myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) , and 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The expression of two major myelin proteins [ myelin basic protein ( MBP ) and proteolipid protein ( PLP ) ] in cerebral cortex ( CTX ) and hippocampus ( HIP ) was decreased by 73 92 % in undernourished control mice , as judged by Northern and Western blot hybridization . ^^^ The abundances of MBP and PLP mRNAs and proteins , however , were decreased by only 40 70 % in undernourished IGF 1 Tg mice . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Active immunization of mice with several different protein components of myelin , including myelin basic protein ( MBP ) , proteolipid protein ( PLP ) and myelin oligodendrocyte glycoprotein ( MOG ) , are capable of eliciting an immune response resulting in the quintessential symptoms of EAE : ascending paralysis involving the tail and then the limbs . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
This was correlated with markedly reduced expression of the myelin proteins , proteolipid protein ( PLP ) , myelin basic protein ( MBP ) , 2 ' , 3 cyclic nucleotide 3 ' phosphodiesterase , and both large ( L ) and small ( S ) isoforms of myelin associated glycoprotein ( MAG ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Myelin proteins , including myelin basic protein ( MBP ) , proteolipid protein ( PLP ) and myelin oligodendrocyte glycoprotein ( MOG ) are candidate autoantigens in MS . ^^^ We evaluated the IFN gamma production induced by MBP and MOG and selected MBP , MOG and PLP peptides in MS patients and healthy controls using the IFN gamma ELISPOT assay . ^^^ No myelin peptide was preferentially recognized among the peptides tested ( MBP 84 102 , 143 168 , MOG 1 22 , 34 56 , 64 86 , 74 96 , PLP 41 58 , 184 199 , 190 209 ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The phenotypes of double mutant mice whose genomes are homozygous for an Mbp ( myelin basic protein ) mutation and hemizygous for a juvenile lethal Plp ( proteolipid protein ) mutation were compared in earlier studies . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Autoreactive T cells specific for candidate myelin antigens , including myelin basic protein ( MBP ) and proteolipid protein ( PLP ) , are thought to play an important role in the pathogenesis of multiple sclerosis ( MS ) . ^^^ In this study , we examined the precursor frequency , the reactivity pattern and cytokine profile of myelin reactive T cells that were primed in vitro with overlapping peptides of MBP and PLP in patients with MS and healthy individuals . ^^^ The results revealed that T cells specific for peptides of MBP and PLP occurred at a relatively higher precursor frequency in patients with MS than that in healthy individuals . ^^^ We identified a number of dominant T cell epitopes within MBP and PLP , some of which were not previously detected using whole myelin antigens as the primary stimuli . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The increase in the size of the sheets is also mirrored by changes in the intensity and distribution of myelin basic protein ( MBP ) and proteolipid protein ( PLP ) mRNAs . ^^^ As compared to controls , the PPAR delta agonist treated groups contain more OLs that have MBP and PLP mRNA extending into distal processes . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Two immortalized stable cell lines , a normal ( 158N ) and jimpy ( 158JP ) cell line , expressed transcripts and proteins of oligodendrocyte markers , including proteolipid protein ( PLP ) , myelin basic protein ( MBP ) , and carbonic anhydrase 2 ( CAII ) . ^^^ While the level of oligodendrocyte markers ( PLP , MBP , and CAII ) were significantly increased by dibutyryl cAMP ( dbcAMP ) treatment in the normal cell line , no changes were observed in the jimpy cell lines . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
To clarify the characteristics of T cells autoreactive to myelin proteins in each MS subtype , we established T cell lines reactive to such myelin antigens as myelin basic protein ( MBP ) , proteolipid protein ( PLP ) and myelin oligodendrocyte glycoprotein ( MOG ) from 5 of 10 OS MS patients , 6 of 11 C MS patients and 7 of 13 healthy controls ( HCs ) , and T cell epitopes and their restriction molecules were determined . ^^^ We found that ( a ) intermolecular epitope spreading was found to be significantly more frequent in MS patients than in HCs ( P=0 . 0128 ) , ( b ) intramolecular epitope spreading also tended to occur more frequently in MS patients than in HCs ( P=0 . 0584 ) , ( c ) in OS MS , HLA DR restricted and MOG autoreactive T cells were more frequently established as compared with those reactive to MBP or PLP epitopes and ( d ) in C MS , HLA DQ restricted and PLP autoreactive T cells dominated those autoreactive to MBP or MOG epitopes . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The classical myelin markers myelin basic protein ( MBP ) and proteolipidic protein ( PLP ) are expressed later in oligodendroglial development , when myelin sheath formation is in progress . ^^^ We assessed the developmental sequence of appearance of CNPase , MBP , MOPB , and PLP proteins in cerebellum ( Cb ) and corpus callosum ( cc ) in an experimental hypothyroidism model . ^^^ The developmental pattern of expression of CNPase , MBP , and PLP proteins was also delayed in the H group . ^^^ CNPase and MBP expression was recovered in cc and Cb , whereas PLP remained below control levels at P 90 in cc . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
MBP , MOG , PLP , MAG ) , the presence of reactive T cells in blood and , further enriched , in the cerebrospinal fluid ( CSF ) , large numbers of B cells in the CSF secreting antibodies of multiple but unknown specificities , an increase of mononuclear cells ( MNC ) expressing and secreting both pro and anti inflammatory cytokines , including Th 1 cytokines interferon gamma ( IFN gamma ) and interleukin ( IL ) 6 , the Th 2 related IL 4 and IL 10 , and the Th 3 driven TGF beta , elevated numbers of MNC in both blood and CSF expressing a spectrum of metalloproteinases and their inhibitors , as well as many other aberrations . ^^^ Furthermore , a complete epitope spread affecting MBP , MOG , PLP , MAG and other myelin components is already present in ON . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Lymphocyte proliferation assays demonstrated a more than twofold decrease in the reactivity to myelin antigens ( MBP and PLP ) and downregulation of the activated lymphocytes ( expressing the CD62L , and IA k MHC Class 1 markers and the Vb 17 T cell receptor ) in the FTS treated group ; in vitro FTS suppressed the Ras activity of lymphocytes and inhibited the proliferative ability of the lymphocytes in a dose dependent manner . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
In contrast to the results obtained using MBP , we found that approximately 50 % of PLP reactive T cells were derived from the CD45RO+ memory subpopulation of T cells isolated from these control subjects . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The isoforms of the main myelin proteins , the peripheral myelin basic isoproteins ( MBP ) and the integral proteolipid proteins , PLP and DM 20 , arise from alternative splicing . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Here we show that in rats that overexpress PLP , impairment of PLP transport from the cell body to the processes interferes with the translocation of other membrane proteins such as myelin associated glycoprotein ( MAG ) and myelin oligodendrocyte glycoprotein ( MOG ) , but not with peripherally translated MBP . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Importantly , myelin specific tolerance induced by intravenous administration of MP 4 , a fusion of the myelin proteins myelin basic protein ( MBP ) and PLP , to SJL mice with ongoing TMEV IDD attenuated disease progression and resulted in significantly less demyelination and decreased inflammatory cell infiltration in the CNS . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Compared to controls treated with saline or PDGF AA , FGF 2 induced an accumulation of PLP protein and MBP mRNA within the somata of myelin producing oligodendrocytes . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Ceramide monohexoside ( CMH ) levels correlated highly with the expression of mRNA for 4 myelin proteins : CGT , MBP , CNP , and PLP . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The pathogenicity of antibodies ( Ab ) to MOG , myelin basic protein ( MBP ) , proteolipid protein ( PLP ) and galactocerebroside ( GalC ) was investigated in vivo following injection at the onset of EAE . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The serial evaluation of antimyelin ( MBP , PLP , MOG ) T cell responses revealed highly dynamic shifts and fluctuations from one pattern to another in a patient dependent manner . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
A synthetic gene was constructed to encode selected disease relevant epitopes of myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , and myelin oligodendrocyte glycoprotein ( MOG ) . ^^^ Systemic administration of hmTAP not only suppressed and treated experimental autoimmune encephalomyelitis ( EAE ) initiated by autoreactivity to a PLP epitope , but also abrogated complex EAE transferred by multispecific line T cells reactive against encephalitogenic epitopes of MBP , PLP , and MOG . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
To evaluate myelination we investigated IGF 1 stimulated expression of myelin basic protein ( MBP ) and proteolipid protein ( PLP ) in the cerebral cortex CTX and brainstem , and found similar increases in each region in IRS 1 ( / ) and wild type mice . ^^^ In studies using mixed glial cultures derived from IRS 1 ( / ) mice , IGF 1 also increased the abundance of MBP and PLP mRNA . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
In this study , we examined myelination during postnatal development in IGF 1 knock out ( KO ) mice by assessing myelin staining , the expression of myelin basic protein ( MBP ) and proteolipid protein ( PLP ) , two major myelin specific proteins , and the number of oligodendrocytes and their precursors . ^^^ We found that myelin staining , MBP and PLP expression , and the percentage of oligodendrocytes and their precursors are significantly reduced in all brain regions of developing IGF 1 KO mice but are similar to controls in adult IGF 1 KO mice . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The lack of PLP is accompanied by deficits in the other myelin proteins of the central nervous system , including myelin basic protein , myelin associated glycoprotein , and cyclic nucleotide phosphodiesterase . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
To further our understanding of the functions of the major myelin proteins , myelin basic protein ( MBP ) and proteolipid protein ( PLP ) , and other myelin proteins , such as 2 ' 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNP ) and myelin associated glycoprotein ( MAG ) , bovine brain myelin was extracted with Triton 10 100 , and protein complexes in the detergent soluble fraction were isolated by coimmunoprecipitation and sucrose density gradient sedimentation . ^^^ MBP , PLP , and the small isoform of MAG ( S MAG ) were coimmunoprecipitated from the detergent soluble fraction by anti PLP , anti MBP or anti MAG monoclonal antibodies . ^^^ Lipid analysis revealed that the PLP MBP S MAG coimmunoprecipitated with some phospholipids and sulfatide but not cholesterol or galactosylceramide . ^^^ Given that MAG is localized mainly in the periaxonal region of myelin , where it interacts with axonal ligands , the PLP MBP S MAG complex may come from these regions , where it could participate in dynamic functions in the myelin sheath and myelin axonal interactions . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
PLP and a second simultaneously upregulated gene , the myelin basic protein ( MBP ) gene , were spatially separated in both progenitors and differentiated oligodendrocytes . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
We found by run off transcription that the MBP mRNA was significantly increased at the nuclear level but the PLP message was unaffected . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Furthermore , a reduction in the expression of myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , and glial fibrillary acidic protein ( GFAP ) , markers for mature oligodendrocytes and astrocytes , was observed in the Ngn 3 null mice . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The TCL showed reactivity to MBP , MOG and / or PLP as tested by Elispot and had a restricted clonality . ^^^ Anti MBP / PLP / MOG reactivities remained low or were reduced in all patients . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
In the chronic stage of PVL , MyT 1 positive cells were significantly increased around necrotic foci and some of the regions were coincident with increasing MBP and PLP immunoreactivity . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Furthermore , we show that NG2+ cells express mRNAs for proteolipid protein ( PLP ) , myelin basic protein , and 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase , but they dot not express DM 20 mRNA , a PLP mRNA splicing variant . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
An immunohistochemical study , which can detect the specific expression of PLP , myelin basic protein , myelin associated glycoprotein , and platelet derived growth factor receptor alpha subunit in brain tissues , showed that the myelination was almost the same as that of age matched controls . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The ELISPOT assay was used to determine the ex vivo interferon gamma ( IFNgamma ) and interleukin 10 ( IL 10 ) production by peripheral blood mononuclear cells ( PBMCs ) in response to peptides spanning the entire proteolipid protein ( PLP ) and myelin basic protein ( MBP ) molecules in 20 patients with relapsing remitting ( RR ) MS and 27 age and sex matched healthy controls . ^^^ MS patients showed significantly higher MBP induced IFNgamma responses and PLP induced IL 10 responses compared with healthy controls . ^^^ Using the Multiple Sclerosis Functional Composite ( MSFC ) , a new multifactorial measure of disability , MS patients showed a significant correlation between the IFNgamma response to PLP peptides and MBP peptides , and disability . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
A panel of recombinant lactobacilli was constructed producing myelin proteins and peptides , including human and guinea pig myelin basic protein ( MBP ) and proteolipid protein peptide 139 151 ( PLP ( 139 151 ) ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
We identified apoptosis and decrease in transcription of the genes for proteolipid protein ( PLP ) and myelin basic protein ( MBP ) in five 1 cm long spinal cord segments ( S 1 , distant rostral ; S 2 , near rostral ; S 3 , lesion ; S 4 , near caudal ; and S 5 , distant caudal ) 24 h after induction of SCI ( 40 g . cm force ) in rats by weight drop method . ^^^ Levels of transcription of PLP and MBP were reduced highly in the lesion and moderately in near segments , suggesting that apoptotic loss of cells impaired biosynthesis of two important structural components of myelin . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
In addition , RA treated CD133+ cells expressed cell type specific markers for oligodendrocytes including myelin basic protein ( MBP ) as shown by RT PCR , proteolipid protein ( PLP ) by Western blot analysis , and cyclic nucleotide phosphodiesterase ( CNPase ) by immunostaining . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Levels of PLP / DM20 and myelin basic protein are considerably greater in myelin fractions from older compared with younger mutants . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
We determined the serum levels of autoantibodies for myelin basic protein ( MBP ) , proteolipid ( PLP ) and myelin oligodendrocyte glycoprotein sequence MOG 92 106 in a group of 54 healthy individuals and 26 MS patients expressing or not HLA DQB1 * 0602 . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The most robust and consistent finding , however , was a decrease in the expression of a number of myelin related genes , including myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , and myelin associated oligodendrocyte basic protein ( MOBP ) . ^^^ The differential expression seen by microarray for CART as well as MBP , MOBP , and PLP was verified by RT PCR . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
RTL 401 given i . v . or s . c . but not empty RTL 400 or free PLP 139 151 peptide prevented relapses and significantly reduced clinical severity of EAE induced by PLP 139 151 peptide in SJL / J or ( C57BL / 6 10 SJL ) F ( 1 ) mice , but did not inhibit EAE induced by PLP 178 191 or MBP 84 104 peptides in SJL / J mice , or MOG 35 55 peptide in ( C57BL / 6 10 SJL / J ) F ( 1 ) mice . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
An immunohistochemical decrease in proteolipid protein ( PLP ) and a more profound deficiency of myelin basic protein ( MBP ) indicate that this lesion is not simply the result of a delay or arrest in myelination and suggests a `` dying back ' ' oligopathy . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Myelin oligodendrocyte glycoprotein ( MOG ) , proteolipid protein ( PLP ) , MAPK , and some phosphotyrosine containing proteins were found to co cluster with GalC and MBP , but myelin associated glycoprotein ( MAG ) and phosphatidylinositol 4 , 5 bisphosphate ( PIP ( 2 ) ) did not . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
T cell epitope spreading has been demonstrated in rodents for myelin basic protein ( MBP ) and proteolipid protein ( PLP ) determinants , but not for myelin oligodendrocyte glycoprotein ( MOG ) , another important myelin antigen . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
In this study , we show that the immune response elicited by immunization with this epitope is qualitatively different from immune responses induced by the well defined epitopes myelin basic protein ( MBP ) 84 96 and proteolipid protein ( PLP ) 139 151 . ^^^ Mice with MOG 92 106 , but not with MBP 84 96 or PLP 139 151 induced experimental autoimmune encephalomyelitis developed extensive B cell reactivity against secondary myelin Ags . ^^^ These secondary Abs were directed against a set of encephalitogenic peptide Ags derived from MBP and PLP as well as a broad range of epitopes spanning the complete MBP sequence . ^^^ In sharp contrast , B cell reactivity in MBP 84 96 and PLP 139 151 induced experimental autoimmune encephalomyelitis was directed against the disease inducing Ag only . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Purified immature rodent oligodendrocytes treated with BMP 4 maintained galactocerebroside ( GalC ) expression , whereas the expression of three key myelin proteins , proteolipid protein ( PLP ) , myelin basic protein ( MBP ) , and 2 ' 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNP ) , was severely decreased . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Demonstration of different myelin proteins ( myelin basic protein [ MBP ] , proteolipid protein [ PLP ] and myelin oligodendrocyte glycoprotein [ MOG ] ) is used as a tool to determine the stage of MS lesions in autopsy tissue . ^^^ The cytospots were immunocytochemically stained with mono and polyclonal antibodies directed against various myelin proteins ( MOG , MBP , PLP ) . ^^^ We found that MOG is degraded after 1 day , whereas PLP and MBP can be detected for a longer period , 2 and 3 days , respectively . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Macrophages containing myelin basic protein ( MBP ) and proteolipid protein ( PLP ) were found in CLN of all MS patients , whereas these could only be detected in a minority of HC . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
These antibodies , which recognize myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , myelin associated glycoprotein ( MAG ) , myelin oligodendrocyte glycoprotein ( MOG ) , oligodendrocyte specific protein ( OSP ) , 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNP ) , and transaldolase ( TAL ) , have been identified mostly in cerebrospinal fluid and in serum . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
A striking exception is a group of myelin related genes , consisting of multiple transcripts representing myelin basic protein ( MBP ) , proteolipid protein ( PLP ) and myelin associated oligodendrocyte basic protein ( MOBP ) , which as a group are substantially decreased in cocaine abusers compared to controls . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The ELISPOT assay was used to determine the ex vivo cytokine production in response to nine amino acid long peptides spanning the entire proteolipid protein ( PLP ) and myelin basic protein ( MBP ) molecules in relapsing remitting ( RR ) MS patients and matched healthy controls . ^^^ We identified three longitudinal levels of myelin induced cytokine secretion by adding up the positive responses for all PLP or MBP peptides obtained for five timepoints , at three month intervals : low reactivity ( < 200 cumulative cytokine secreting cells ) , isolated peptide reactivity ( 201 450 cumulative cytokine secreting cells ) and recurrent protein wide bursts of cytokine reactivity ( > 451 cumulative cytokine secreting cells ) . ^^^ The majority of MS patients showed recurrent bursts to PLP and MBP . ^^^ The majority of patients with gad enhancing lesions showed PLP / IFN gamma and MBP / IFN gamma recurrent burst responses . ^^^ This is the first longitudinal study on MS patients in which nine amino acid long myelin peptides are used to reveal the broad range of PLP and MBP peptide cytokine reactivity across the whole molecule of these two major myelin proteins . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
CGT deficient mice expressing the PLP CGT transgene did not show any behavioral abnormalities , normal myelin structure , and MBP levels . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Quantitative RT PCR was used to determine if P 0 overexpression altered expression of other myelin related genes compared with glial fibrillary acidic protein ( GFAP ) ; the ratios of myelin basic protein ( MBP ) / GFAP and proteolipid protein ( PLP ) / GFAP were increased 2 to 3 fold in the P 0 transfected cells . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
To understand if proinflammatory mediators such as IL ( interleukin ) 1beta and TNF ( tumor necrosis factor ) alpha are capable of modulating the expression of myelin specific genes , we investigated the effect of these cytokines on the expression of myelin basic protein ( MBP ) , 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNPase ) , myelin oligodendrocyte glycoprotein ( MOG ) , and proteolipid protein ( PLP ) in human primary oligodendrocytes . ^^^ Interestingly , both IL 1beta and TNF alpha markedly inhibited the expression of MOG , CNPase , and PLP but not MBP , the effect that was blocked by antioxidants such as N acetylcysteine ( NAC ) and pyrrolidine dithiocarbamate ( PDTC ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
These proteins include one to several alternatively spliced myelin basic protein ( MBP ) isoforms in all sheaths , proteolipid protein ( PLP ) and DM 20 ( except in amphibians ) in tetrapod CNS sheaths , and one or two protein zero ( P 0 ) isoforms in fish CNS and in all vertebrate PNS sheaths . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The prominent autoimmune etiology of MS is considered to be the aberrant activation of IFN gamma producing Th 1 cells that recognize self peptides of the myelin sheath , such as myelin basic protein ( MBP ) and proteolipid protein ( PLP ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Much of it stems from models of experimental autoimmune encephalomyelitis , generated by inoculation of animals with central nervous system antigens such as MBP , PLP , S 100 and MOG or peptides thereof . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Immunohistochemical studies revealed significantly reduced levels of PLP / DM20 and other major myelin components such as myelin basic protein ( MBP ) , myelin associated glycoprotein ( MAG ) , and 2 ' , 3 ' cyclic nucleotide 3 ' phosphodiesterase ( CNP ) in shp myelin . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
MBP and PLP are major structural protein components of myelin . ^^^ Immune responses to MBP and PLP have been implicated in the pathogenesis of multiple sclerosis ( MS ) , an auto immune disease of the central nervous system . ^^^ We have prepared recombinant forms of MBP and PLP stably expressed in CHO cells . ^^^ Several clones with proper cytoplasmic MBP or surface PLP localization were obtained and characterized by flow cytometry and indirect immunostaining . ^^^ CHO cells expressing the recombinant forms of MBP and PLP can be very useful in studies on the autoimmune mechanism of MS . . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Astrocytes have been shown to present myelin basic protein ( MBP ) and proteolipid protein ( PLP ) to T cells , but it has remained unresolved whether astrocytes present myelin oligodendrocyte glycoprotein ( MOG ) , which has been implicated as an important autoantigen in MS . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
In the brain of 14 day old transgenic mice , the DM 20 mRNA level was decreased , whereas the PLP , MBP , CNP , and MAG mRNA levels were increased . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
These results were confirmed by : ( 1 ) myelin basic protein ( MBP ) immunohistochemistry ; ( 2 ) western blotting of myelin associated glycoprotein ( MAG ) and MBP ; ( 3 ) qRT PCR analyses of myelin associated oligodendrocytic basic protein ( MOBP ) , MAG , MBP and proteolipid protein ( PLP ) in hippocampus , cortex and spinal cord ; ( 4 ) quantitation of ethanolamine and choline plasmalogens , all core myelin components ; ( 5 ) evaluation of myelin content in brain sections employing toluidine blue staining ; and ( 6 ) ultrastructural evaluation of myelin sheath thickness via electron microscopy . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Similarly , increased myelin expression was seen in brain sections from hypothermia plus NAC group , when stained for Luxol Fast Blue ( LFB ) , Myelin Basic Protein ( MBP ) and Proteolipid protein ( PLP ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Using 51Cr release and tetramer staining assays , we found that the CTL response against VP 1 , the major capsid protein of the polyomavirus JC ( JCV ) , was significantly higher than the one against epitopes of MBP and PLP . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Biochemical analyses showed decreased levels of myelin basic protein ( MBP ) , proteolipid protein ( PLP ) , and glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
This study identifies that protease M / neurosin in OLGs is closely associated with the expression of the MBP and the PLP gene . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
At the molecular level , we find that Olig 1 regulates transcription of the major myelin specific genes , Mbp , Plp 1 , and Mag , and suppresses expression of a major astrocyte specific gene , Gfap . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
The myelin basic protein ( MBP ) gene , which encodes the second most abundant CNS myelin protein after PLP 1 , presents rearrangements in hypomyelinating murine mutants and is always included in the minimal region deleted in 18q patients with an abnormal hypomyelination pattern on cerebral MRI . ^^^ In this study , we looked at the genomic copy number at the Golli MBP locus in 195 patients with cerebral MRI suggesting a myelin defect , who do not have PLP 1 mutation . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Here we show that human QKI mRNA levels can account for a high proportion ( 47 % ) of normal interindividual mRNA expression variation ( and covariation ) of six oligodendrocyte related genes ( PLP 1 , MAG , MBP , TF , SOX 10 , and CDKN1B ) in 55 human brain autopsy samples from individuals without psychiatric diagnoses . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
Expression of genes corresponding to proteolipid protein ( PLP / DM20 ) , myelin protein zero ( P 0 ) , and myelin basic protein ( MBP ) is detected at 2 days postfertilization ( dpf ) , first in the ventral hindbrain , close to the midline . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
We studied the myelination of the visual pathway during the ontogeny of the lizard Gallotia galloti using immunohistochemical methods to stain the myelin basic protein ( MBP ) and proteolipid protein ( PLP / DM20 ) , and electron microscopy . ^^^ The staining pattern for the PLP / DM20 and MBP overlapped during the lizard ontogeny and was first observed at E 39 in cell bodies and fibers located in the temporal optic nerve , optic chiasm , middle optic tract , and in the stratum album centrale of the optic tectum ( OT ) . ^^^
Interacting proteins: P60201 and P02686	Pubmed	SVM Score :0.0
One case of each of the following human leukodystrophies was examined immunocytochemically with antisera against myelin basic protein ( MBP ) , myelin associated glycoprotein ( MAG ) and gliofibrillary acidic protein ( GFA ) : Metachromatic leukodystrophy ( MLD ) , connatal adrenoleukodystrophy ( ALD ) , sudanophilic leukodystrophy of the adult ( SLD ) and connatal Pelizaeus Merzbacher disease ( PMD ) . ^^^ In PMD , MBP is present in the vicinity of axons not surrounded by a myelin sheath demonstrable with conventional means . ^^^