| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Chloroform induction of ornithine decarboxylase antizyme ( ODC AZ ) in male rat liver . ^^^ ODC AZ was determined by measuring the inhibition of isolated ODC activity as described by Hayashi and Fujita and modified in our laboratory to use kidney ODC . ^^^ Chloroform induced ODC AZ activity in males at 3 and 7 d ( 26 % and 37 % inhibition of the ODC activity in the incubation medium , respectively ) . ^^^ While females exhibited a similar decline in ODC activity after repeated doses , ODC AZ was not induced . ^^^ Thus , it would appear that daily exposure of rats to chloroform results in a refractoriness of its induction of ODC activity accompanied by an induction of the ODC AZ in males . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Recent studies have provided convincing evidence to add to a number of earlier observations suggesting that the rapid intracellular degradation of mammalian ornithine decarboxylase ( ODC ) is further accelerated by the action of ornithine decarboxylase antizyme ( ODC Az ) , a polyamine induced protein . ^^^ However , the mechanism whereby ODC Az exerts its effect in this proteolytic process is mostly unknown . ^^^ Here , by using reticulocyte lysate based synthesis and degradation systems , we demonstrate that interaction of ODC Az with ODC results in two related outcomes : ( a ) ODC is inactivated as a result of its monomerization , and ( b ) ODC degradation is dramatically accelerated . ^^^ While ODC inactivation requires the integrity of the ODC Az binding site of ODC and the ODC binding site of ODC Az , acceleration in ODC degradation also requires the previously characterized carboxyl terminal destabilizing segment of ODC and a specific segment of ODC Az that may be functionally distinct from that required for ODC binding . ^^^ Interestingly , an active ODC variant with a mutant ODC Az binding site is stable under basal degradation conditions . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Reduction of ornithine decarboxylase antizyme ( ODC Az ) level in the 7 , 12 dimethylbenz ( a ) anthracene induced hamster buccal pouch carcinogenesis model . ^^^ The hamster cDNA is homologous to the human , mouse and rat ornithine decarboxylase antizyme gene ( ODC Az ) . ^^^ The hamster ODC Az gene demonstrated a restriction fragment length polymorphism ( RFLP ) upon Southern blot analysis comparing normal and tumor hamster genomic DNA . ^^^ Northern blot analysis revealed that normal hamster oral keratinocytes express readily detectable level of ODC Az mRNA . ^^^ Malignant oral keratinocytes demonstrate reduced expression of the ODC Az mRNA . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Ornithine decarboxylase antizyme is a normal component of uninduced H 35 cells and rat liver . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Seven monoclonal antibodies were prepared against rat liver ornithine decarboxylase antizyme , a unique regulatory protein of the enzyme induced by its products . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Studies on ornithine decarboxylase antizyme . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Ornithine decarboxylase antizyme in mammalian tissues . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Difluoromethylornithine does not bind to ornithine decarboxylase antizyme complex . alpha Difluoromethylornithine ( DFMO ) has been widely used for determining the amounts of ornithine decarboxylase ( ODC ) in cells . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Ornithine decarboxylase antizyme in kidneys of male and female mice . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| No induction of ornithine decarboxylase antizyme by 2 methylputrescine could be detected . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Properties of ornithine decarboxylase antizyme from mammary gland of lactating rats . ^^^ Ornithine decarboxylase antizyme was induced in mammary gland of fasted lactating rats by administration of 1 , 3 diaminopropan 2 ol . ^^^ However , ornithine decarboxylase antizyme from mammary gland eluted from Sephadex G 75 with an apparent molecular mass of 55 kDa , compared with 27 kDa , for antizyme from liver under identical conditions . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Cloning and characterization of a rat gene encoding ornithine decarboxylase antizyme . ^^^ We cloned an ornithine decarboxylase antizyme encoding gene ( Oaz ) from a rat liver genomic library . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Ornithine decarboxylase antizyme : a regulatory factor of the enzyme degradation ] . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Presence of ornithine decarboxylase antizyme in primary cultured hepatocytes of the frog Xenopus laevis . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Analyses of ornithine decarboxylase antizyme mRNA with a cDNA cloned from rat liver . ^^^ Ornithine decarboxylase antizyme is a unique inhibitory protein induced by polyamines and involved in the regulation of ornithine decarboxylase . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Some evidence for inactivation through ornithine decarboxylase antizyme complex formation was obtained . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Modulation of ornithine decarboxylase activity and ornithine decarboxylase antizyme complex in rat heart by hormone and putrescine treatment . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| In addition , mammalian cells contain an anti antizyme , a protein that specifically binds to the antizyme of an ornithine decarboxylase antizyme complex and liberates free ornithine decarboxylase from the complex . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Accumulation of ornithine decarboxylase antizyme complex in HMOA cells . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Existence of antizyme and ornithine decarboxylase antizyme complex in RK 13 kidney cells . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Cell cycle phase dependent induction of ornithine decarboxylase antizyme . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Effect of starvation and refeeding on polyamine concentrations and ornithine decarboxylase antizyme in mammary gland of lactating rats . ^^^ Starvation caused a marked increase in putrescine content in mammary gland of lactating rats , together with a marked decrease in activity of ornithine decarboxylase and appearance of measurable ornithine decarboxylase antizyme . 2 . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Immunoadsorption on a column of the monoclonal antibody Sepharose 4B was shown to be useful for the removal of ornithine decarboxylase from antizyme inhibitor preparations , an essential procedure for the accurate assay of either ornithine decarboxylase antizyme complex or antizyme inhibitor . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Diaminopropane inhibits brain ornithine decarboxylase , but does not induce an ornithine decarboxylase antizyme . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Permanent decrease in activity of ornithine decarboxylase antizyme in rat liver during chemical hepatocarcinogenesis . ^^^ The formation of hepatic ornithine decarboxylase antizyme was elicited by i . p . injections of putrescine into rats fed a basal diet and rats fed the carcinogenic diet . ^^^ The activities of both hepatic ornithine decarboxylase and hepatic ornithine decarboxylase antizyme were measured every month for five months , i . e . , until hepatoma was fully developed . ^^^ During azo dye hepatocarcinogenesis and in fully developed hepatoma the activity of hepatic ornithine decarboxylase antizyme was always significantly lower than in normal resting liver , with minima at the second and the third months . ^^^ The hepatoma does not synthesize ornithine decarboxylase antizyme more slowly than normal liver , since the difference could be neither abolished nor lessened by lengthening the time available for antizyme formation . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Restoration of normal ornithine decarboxylase antizyme activity in rat liver after acute carcinogen treatment . ^^^ This study was undertaken to see whether or not the decrease in ornithine decarboxylase antizyme activity caused in rat liver by a hepatocarcinogen could be reversed . ^^^ The activities of both hepatic ornithine decarboxylase and hepatic ornithine decarboxylase antizyme were measured at intervals of hours after the injection of thioacetamide . ^^^ The hepatic ornithine decarboxylase antizyme in thioacetamide treated rats was minimal at 40 and 80 h after carcinogen administration . ^^^ The reversal process requires a very long time , namely 450 h for normal levels of hepatic ornithine decarboxylase antizyme activity to be restored in treated rats . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| A macromolecular inhibitor of ornithine decarboxylase that has been termed antizyme is induced in response to 1 , 3 diaminopropane , but our results indicate that the loss of enzyme activity is not due to the accumulation of inactive ornithine decarboxylase antizyme complexes . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Detection of ornithine decarboxylase antizyme in mouse brain . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Autoregulatory frameshifting in decoding mammalian ornithine decarboxylase antizyme . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Physiological function of ornithine decarboxylase antizyme ] . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Polyamines regulate the expression of ornithine decarboxylase antizyme in vitro by inducing ribosomal frame shifting . ^^^ Conventional reading of the sequence of ornithine decarboxylase antizyme mRNA ( a protein that modulates the rate of ornithine decarboxylase degradation ) results in premature termination at an in frame termination codon ( stop 1 ) , located shortly after the initiation codon . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Functional regions of ornithine decarboxylase antizyme . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Identification , cloning , and nucleotide sequencing of the ornithine decarboxylase antizyme gene of Escherichia coli . ^^^ The ornithine decarboxylase antizyme gene of Escherichia coli was identified by immunological screening of an E . coli genomic library . ^^^ These data strongly suggest that ( 1 ) the cloned gene encodes the ornithine decarboxylase antizyme and ( 2 ) antizyme is a bifunctional protein serving as both an inhibitor of polyamine biosynthesis as well as a transcriptional regulator of an as yet unknown set of genes . . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| The expression of ornithine decarboxylase antizyme mRNA and protein in rat motoneurons . ^^^ The distribution of ornithine decarboxylase antizyme messenger ribonucleic acid ( AZ mRNA ) and AZ like immunoreactivity ( LI ) was studied in the brainstem and spinal cord motoneurons and in the extraocular and triceps surae muscles of rat . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Programmed translational frameshifting is essential for the expression of mammalian ornithine decarboxylase antizyme , a protein involved in the regulation of intracellular polyamines . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| A frameshift in a mammalian chromosomal gene , ornithine decarboxylase antizyme , has recently been reported ; 5 ' sequences have been shown to be vital for this frameshift event . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Nucleotide sequence of ornithine decarboxylase antizyme cDNA from Xenopus laevis . ^^^ An ornithine decarboxylase antizyme cDNA was obtained from Xenopus laevis liver and its sequence was determined . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| These genes were chromogranin B , intracisternal A particle envelope , ornithine decarboxylase antizyme , heat shock protein 70 and one unidentified gene . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Molecular cloning and sequencing of a human cDNA encoding ornithine decarboxylase antizyme . ^^^ We report the cloning of a cDNA encoding the human homolog of ornithine decarboxylase antizyme from a human gingival fibroblast cDNA library . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Ornithine decarboxylase antizyme : a novel type of regulatory protein . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| The guf gene was cloned and found to encode a 21 kD protein with a significant sequence similarity to the mammalian ornithine decarboxylase antizyme ( OAZ ) . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| We have cloned the cDNA encoding the human ornithine decarboxylase antizyme from a 5 ' stretch cDNA library of human B cell lymphoma Daudi . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Structure , organization and expression of the mouse ornithine decarboxylase antizyme gene . ^^^ Ornithine decarboxylase antizyme is a protein that participates in the regulation of cellular polyamine levels . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Cloning and sequencing of a human cDNA encoding ornithine decarboxylase antizyme inhibitor . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| The other two cDNA fragments representing human elongation factor 1 alpha ( HEF 1 alpha ) and ornithine decarboxylase antizyme ( OAz ) were overexpressed in PCI 06A Raf ( K ) transfectants compared with PCI 06A Raf ( AS ) transfectants . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Polyamines regulate both transcription and translation of the gene encoding ornithine decarboxylase antizyme in mouse . ^^^ These results indicate a role for polyamines in the transcriptional and translational regulation of ornithine decarboxylase antizyme . . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Osmotic stress induces variation in cellular levels of ornithine decarboxylase antizyme . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Previously , a Drosophila melanogaster sequence with high homology to the sequence for mammalian antizyme ( ornithine decarboxylase antizyme ) was reported . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| The chicken cDNA for ornithine decarboxylase antizyme . ^^^ Two full length avian cDNAs for ornithine decarboxylase antizyme were isolated from a chicken cochlear cDNA library and differed in length through use of alternative poly ( A ) addition signals . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| The coding sequence for mammalian ornithine decarboxylase antizyme is in two different partially overlapping reading frames with no independent ribosome entry to the second ORF . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| A second mammalian ornithine decarboxylase antizyme was discovered . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| The elevation of cellular polyamine levels on the other hand accelerates the induction of ornithine decarboxylase antizyme ( antizyme ) , which is involved not only in ODC degradation , but in the negative regulation of polyamine transport . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Conversely , ornithine decarboxylase antizyme transcript dropped to its lowest level at 12 h , while reaching its highest values between 24 and 48 h . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Ornithine decarboxylase antizyme 1 , a negative regulator of polyamine levels , acts by destabilizing the first enzyme in the polyamine biosynthetic pathway ( ODC ) and by inhibiting uptake of polyamines . ^^^ Structure of human ornithine decarboxylase antizyme 2 gene . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Ornithine decarboxylase antizyme levels , measured by Western blotting , were elevated 1 2 h after spermine treatment of spermine sensitive cells but not spermine resistant cells . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Discovery of a spermatogenesis stage specific ornithine decarboxylase antizyme : antizyme 3 . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Identification and characterization of testis specific ornithine decarboxylase antizyme ( OAZ t ) gene : expression in haploid germ cells and polyamine induced frameshifting . ^^^ RESULTS : We have cloned a testis specific gene encoding an ornithine decarboxylase antizyme known to control intracellular concentrations of polyamines in a feedback manner . ^^^ The mRNA encoding the protein named ornithine decarboxylase antizyme in testis ( OAZ t ) was specifically expressed in haploid germ cells . ^^^ In contrast , the mRNA level of somatic ornithine decarboxylase antizyme 1 ( OAZ 1 ) decreased markedly at the late stages of haploid germ cell differentiation . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Ornithine decarboxylase antizyme ( OAZ ) , induced by high polyamine levels , inhibits ODC activity and prevents extracellular polyamine uptake . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| The hamster ornithine decarboxylase antizyme ( ODC Az ) cDNA was transfected into the hamster malignant oral keratinocyte cell line , HCPC 1 . ^^^ Induction of epithelial differentiation and DNA demethylation in hamster malignant oral keratinocyte by ornithine decarboxylase antizyme . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| The targeting of Smad 1 to proteasome involves not only the receptor activation induced Smad 1 ubiquitination but also the targeting functions of the ornithine decarboxylase antizyme and the proteasome beta subunit HsN 3 . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Ornithine decarboxylase antizyme is rapidly degraded through a mechanism that requires functional ubiquitin dependent proteolytic activity . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| P elements inserted in the vicinity of or within the Drosophila snRNP SmD 3 gene nested in the first intron of the Ornithine Decarboxylase Antizyme gene affect only the expression of SmD 3 . ^^^ The Drosophila gene for snRNP SmD 3 ( SmD 3 ) is contained in reverse orientation within the first intron of the Ornithine Decarboxylase Antizyme ( AZ ) gene . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Smad 1 forms a complex with a proteasome beta subunit HsN 3 and the ornithine decarboxylase antizyme ( Az ) . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| The protein ornithine decarboxylase antizyme ( AZ ) is inhibitory to both polyamine transport and synthesis . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Structure and promoter activity of the gene encoding ornithine decarboxylase antizyme expressed exclusively in haploid germ cells in testis ( OAZt / Oaz3 ) . ^^^ Ornithine decarboxylase antizyme 1 and 2 ( OAZ 1 and OAZ 2 ) are expressed ubiquitously , and control the intracellular concentration of polyamines . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| In an experiment aimed at identifying targets of Sex lethal in early germ cells , the RNA encoded by gutfeeling , the Drosophila homolog of Ornithine Decarboxylase Antizyme , was isolated . gutfeeling interacts genetically with Sex lethal . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Through the interaction with proteasome beta subunit HsN 3 and the substrate marker protein ornithine decarboxylase antizyme ( AZ ) , the BMP responsive Smad 1 regulates the proteasomal targeting events that contribute to the degradation of Smad 1 and its interacting proteins , one of which is SNIP 1 , a repressor of the transcriptional co activator CBP / p300 . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Smad 1 and Smad 5 related with transcription factors included core binding factor A 1 ( CBFA 1 ) , smad interacting protein 1 ( SIP 1 ) , ornithine decarboxylase antizyme ( OAZ ) , activating protein 1 ( AP 1 ) , xenopus ventralizing homeobox protein 2 ( Xvent 2 ) , sandostatin ( Ski ) , antiproliferative proteins ( Tob ) , and homeodomain containing transcriptian factor 8 ( Hoxc 8 ) , et al . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| This antiproliferative action was not due to an effect on ornithine decarboxylase antizyme , since its intracellular concentration was not significantly changed by agmatine . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Transient expression analysis of the mouse ornithine decarboxylase antizyme haploid specific promoter using in vivo electroporation . ^^^ The testicular isoform of the ornithine decarboxylase antizyme ( OAZt ) gene is expressed exclusively in the haploid spermatids of mice . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Transgenic expression of ornithine decarboxylase antizyme has suggested that this unique protein may act as a general tumor suppressor . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| The current version of FSFinder is capable of finding 1 frameshift sites on heptamer sequences 10 XXY YYZ , and +1 frameshift sites for two genes : protein chain release factor B ( prfB ) and ornithine decarboxylase antizyme ( oaz ) . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| We found that gametogenetin binding protein 1 ( GGNBP 1 ) , gametogenetin binding protein 2 ( GGNBP 2 ) and ornithine decarboxylase antizyme 3 ( OAZ 3 ) were potential GGN 1 interaction partners . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| The data revealed that both FGF 2 and HA 12 promoted endothelial cell morphogenesis in a process depending on the expression of ornithine decarboxylase ( Odc ) and ornithine decarboxylase antizyme inhibitor ( Oazi ) genes . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Ornithine decarboxylase antizyme ( Az ) , a polyamine induced protein that targets ornithine decarboxylase ( ODC ) to rapid degradation , is synthesized as two isoforms . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Characterization of a counterpart to Mammalian ornithine decarboxylase antizyme in prokaryotes . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| An autoregulatory translational shift to the +1 frame is required for the expression of ornithine decarboxylase antizyme from fungi to mammals . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Identification of polymorphisms and balancing selection in the male infertility candidate gene , ornithine decarboxylase antizyme 3 . ^^^ Ornithine decarboxylase antizyme 3 ( OAZ 3 ) is a testis specific antizyme paralog and the only antizyme expressed in the mid to late stages of spermatogenesis . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| We describe the cloning of a mouse AZI cDNA , encoding a protein with high homology to mouse ODC . ^^^ Using purified recombinant proteins , we show that AZI ( which has no ODC activity ) can release enzymically active ODC from antizyme suppression in vitro . ^^^ We also show that ODC reactivation takes place in mouse fibroblasts upon transient transfection with an AZI expressing plasmid construct . ^^^ Finally we demonstrate that the AZI mRNA content of mouse fibroblasts increases significantly within an hour of growth stimulation , i . e . much earlier than ODC transcripts . ^^^ Our results indicate that induction of AZI synthesis may represent a means of rescuing ODC molecules that have been inactivated and tagged for degradation by antizyme , when culture conditions improve and polyamine production is needed for cell growth and proliferation . . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| We have cloned a cDNA for a novel human protein that is 54 % identical to ODC and 45 % identical to antizyme inhibitor ( AZI ) . mRNA for ODC paralogue ( ODC p ) was found only in the central nervous system and testes , suggesting a role in terminal differentiation rather than cell proliferation . ^^^ ODC p might also function as a brain and testis specific AZI . . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| As described previously , in a degradation system utilizing rabbit reticulocyte lysate , antizyme 1 ( AZ 1 ) accelerates proteasomal ornithine decarboxylase ( ODC ) degradation , but antizyme 2 ( AZ 2 ) does not . ^^^ AZ 1 binds to ODC with about a 3 fold higher potency than AZ 2 , but this can not account for their distinct degradative activities . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Ornithine decarboxylase ( ODC ) is regulated by its metabolic products through a feedback loop that employs a second protein , antizyme 1 ( AZ 1 ) . ^^^ AZ 1 accelerates the degradation of ODC by the proteasome . ^^^ Our results demonstrate that AZ 1 acts on ODC to enhance the association of ODC with the proteasome , not the rate of its processing . ^^^ Substrate linked or free polyubiquitin chains compete for AZ 1 stimulated degradation of ODC . ^^^ ODC AZ 1 is therefore recognized by the same element ( s ) in the proteasome that mediate recognition of polyubiquitin chains . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| In this study , we document the time course effect of testosterone on 1 ) gene expression of ODC , antizyme 1 ( AZ 1 ) , and spermidine / spermine N 1 acetyltransferase ( N 1 SSAT ) ; 2 ) ODC activity in proximal convoluted tubules ( PCT ) and cortical proximal straight tubules ( CPST ) ; and 3 ) renal polyamine levels . ^^^ Surprisingly , although ODC protein and activity were undetectable in female kidneys , the levels of AZ 1 mRNA and protein were similar to those in males . ^^^ Therefore , one may propose that ODC protein could be continuously degraded by AZ 1 in female kidneys . . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Antizyme 1 ( AZ 1 ) is a protein that negatively regulates polyamine synthesis by inhibiting the key synthetic enzyme ornithine decarboxylase and targeting it for degradation by the 26 S proteasome . ^^^ |
|
| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Antizyme 1 has been shown to be negatively regulated through the AZI ( antizyme inhibitor ) that binds antizyme 1 with higher affinity compared with ODC . ^^^ In the present study , we show by yeast two and three hybrid protein protein interaction studies that AZI interacts with all members of the antizyme family and is capable of disrupting the interaction between each antizyme and ODC . ^^^ The protein interactions could be confirmed by immunoprecipitation of the human ODC antizyme 2 AZI complexes . ^^^ In summary , we conclude that human AZI is capable of acting as a general inhibitor for all members of the antizyme family and that the previously not yet characterized antizyme 4 is capable of binding ODC and inhibiting its enzymic activity similar to the other members of the antizyme family . . ^^^ |
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| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| The enzyme ornithine decarboxylase ( ODC ) and its regulatory protein antizyme 1 ( AZ 1 ) are key regulators in the homeostasis of polyamines . ^^^ To gain more insight into the exact intracellular distribution of ODC and AZ 1 , we performed immunocytochemical and Green Fluorescent Protein fluorocytochemical studies in cultured human cervix carcinoma and human prostatic carcinoma ( PC 346C ) cells . ^^^ ODC localization patterns varied from predominantly cytoplasmic to both cytoplasmic and nuclear staining , whereas AZ 1 was mostly found in the nucleus . ^^^ In cells that were synchronized in the mitotic phase , localization of both ODC and AZ 1 changed from perinuclear at the beginning of mitosis into nucleoplasmic at close proximity to the chromosomes during meta , ana and telophase . ^^^ Upon completion of mitosis , localization of ODC and AZ 1 was reverted back to the cytoplasm , i . e . , predominantly perinuclear immediately after cytokinesis . ^^^ |
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| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Antizyme has the ability to bind and inhibit the enzyme ornithine decarboxylase ( ODC ) , targeting it for degradation at the proteasome ; antizyme is also known to affect the transport of polyamines and interact with the antizyme inhibitor protein ( AZI ) , as well as the cell cycle protein cyclin D 1 . ^^^ |
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| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Antizyme inhibitor ( AzI ) is a homolog of ornithine decarboxylase ( ODC ) , a key enzyme of polyamine synthesis . ^^^ AzI overexpression resulted in elevation of ODC activity and of polyamine uptake . ^^^ |
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| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| In contrast to the considerable interest in the oncogene ornithine decarboxylase ( ODC ) and in the family of antizymes with regard to cell proliferation and tumorigenesis , the endogenous antizyme inhibitor ( AZI ) has been less well studied . ^^^ AZI is highly homologous to the enzyme ODC but does not possess any decarboxylase activity . ^^^ AZI inactivates all members of the antizyme family , reactivates ODC and prevents the proteolytic degradation of ODC , which may suggest a role for AZI in tumor progression . . ^^^ |
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| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Degradation of yeast ODC by the proteasome depends on Oaz 1 . ^^^ We propose a model , in which polyamines inhibit their ODC mediated biosynthesis by two mechanisms , the control of Oaz 1 synthesis and inhibition of its degradation . . ^^^ |
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| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Antizyme inhibitor ( AzI ) is an ODC related protein that does not retain enzymatic activity yet binds Az with higher affinity than ODC . ^^^ We show here that like ODC , AzI is also a short lived protein that undergoes proteasomal degradation . ^^^ However , in contrast to ODC degradation , the degradation of AzI is ubiquitin dependent and does not require interaction with Az . ^^^ Substituting the C terminus of AzI with that of ODC , which together with Az constitutes the complete degradation signal of ODC , does not subvert AzI degradation from the ubiquitin dependent mode to the Az dependent mode , suggesting dominance of the ubiquitination signal . ^^^ Our results suggest opposing roles of Az in regulating the degradation of AzI and ODC . . ^^^ |
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| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| Moreover , the antizyme inhibitor ( AZI ) serves as another factor in regulating ODC , by binding to antizyme and releasing ODC from ODC antizyme complexes . ^^^ Therefore , to evaluate the role of AZI in regulating ODC activity in tumors , we successfully down regulated AZI expression using RNA interference technology in A 549 lung cancer cells expressing high levels of AZI . ^^^ Using biological assays , antizyme activity increased in AZI siRNA transfected cells , and ODC levels and activity were reduced as well . ^^^ Our results directly demonstrate that downregulation of AZI regulates ODC activity , intracellular polyamine levels , and cell growth through regulating antizyme activity . ^^^ This study also suggests that highly expressed AZI may be partly responsible for increased ODC activity and cellular transformation . . ^^^ |
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| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P54368 and P11926 |
Pubmed |
SVM Score :0.0 |
| NA |
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