| Interacting proteins: P53567 and P49715 |
Pubmed |
SVM Score :0.0 |
| Further analysis of C / EBP beta and C / EBP gamma . ^^^ We isolated two cDNAs which were found to correspond to leucine zipper proteins of the CC AAT / enhancer binding protein ( C / EBP ) family : C / EBP beta and C / EBP gamma . ^^^ In addition , C / EBP beta and C / EBP gamma readily heterodimerize with each other as well as with C / EBP alpha . ^^^ The mRNAs coding for C / EBP beta and C / EBP gamma are expressed in a wider variety of rat tissues than C / EBP alpha mRNA , including yolk sac and fetal liver . ^^^ |
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| Interacting proteins: P53567 and P49715 |
Pubmed |
SVM Score :0.0 |
| Ig / EBP ( C / EBP gamma ) is a transdominant negative inhibitor of C / EBP family transcriptional activators . ^^^ Analysis of cDNA and genomic clones shows that the murine Ig / EBP ( C / EBP gamma ) gene encodes a small protein with a predicted molecular weight of 16 . 4 kDa which contains C / EBP family basic and leucine zipper domains but lacks the transcriptional activation domains present in C / EBP ( C / EBP alpha ) and NF IL 6 ( C / EBP beta ) . ^^^ |
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| Interacting proteins: P53567 and P49715 |
Pubmed |
SVM Score :0.0 |
| Before PH , the expressions of C / EBP alpha , C / EBP beta , and C / EBP gamma were similar in the two treatment groups . ^^^ After PH , in control rats , mRNA and nuclear protein concentrations of C / EBP beta and C / EBP gamma increased approximately fivefold by 3 hours after PH . ^^^ |
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| Interacting proteins: P53567 and P49715 |
Pubmed |
SVM Score :0.0 |
| Cloning of the cDNA encoding human C / EBP gamma , a protein binding to the PRE 1 enhancer element of the human interleukin 4 promoter . ^^^ The deduced amino acid ( aa ) sequence of HC / EBP gamma contains 150 aa with high homology to mouse Ig / EBP 1 and rat C / EBP gamma . ^^^ |
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| Interacting proteins: P53567 and P49715 |
Pubmed |
SVM Score :0.0 |
| Activation of this cell line results in altered expression of several nuclear DNA binding complexes involving two members of the C / enhancer binding protein ( EBP ) family of transcription factors , namely C / EBP beta ( nuclear factor ( NF ) IL 6 / LAP ) and Ig / EBP 1 ( C / EBP gamma ) . ^^^ |
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| Interacting proteins: P53567 and P49715 |
Pubmed |
SVM Score :0.0 |
| The C / EBP family consist of several related proteins , C / EBP alpha , C / EBP beta , C / EBP gamma , and C / EBP delta , that form homodimers and that form heterodimers with each other . ^^^ |
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| Interacting proteins: P53567 and P49715 |
Pubmed |
SVM Score :0.0 |
| We report the isolation and characterization of genomic and cDNA clones encoding avian CCAAT / enhancer binding protein gamma ( C / EBP gamma ) . ^^^ Sequence analysis indicates that this gene encodes the avian homolog of C / EBP gamma . ^^^ As with the murine C / EBP gamma homolog , the avian C / EBP gamma gene is comprised of two exons , with the open reading frame encoded in exon 2 . ^^^ The 150 aa C / EBP gamma protein is highly conserved , as the avian protein shows more than 80 % identity with the murine and human homologs . ^^^ In vitro transcription and translation assay of this avian cDNA followed by radioimmunoprecipitation assay using a murine C / EBP gamma antiserum indicates that this non optimal initiation codon is used to express a 22 kDa DNA binding protein . . ^^^ |
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| Interacting proteins: P53567 and P49715 |
Pubmed |
SVM Score :0.0 |
| Although other members of the C / EBP family are able to bind the element , very little cooperativity was seen with C / EBP alpha and none with C / EBP gamma . ^^^ In fact , C / EBP gamma was able to inhibit IL 4 induced reporter activity . ^^^ |
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| Interacting proteins: P53567 and P49715 |
Pubmed |
SVM Score :0.0 |
| C / EBP gamma has a stimulatory role on the IL 6 and IL 8 promoters . ^^^ CCAAT / enhancer binding protein gamma ( C / EBP gamma ) is an ubiquitously expressed member of the C / EBP family of transcription factors that has been shown to be an inhibitor of C / EBP transcriptional activators and has been proposed to act as a buffer against C / EBP mediated activation . ^^^ We have now unexpectedly found that C / EBP gamma dramatically augments the activity of C / EBP beta in lipopolysaccharide induction of the interleukin 6 and interleukin 8 promoters in a B lymphoblast cell line . ^^^ This activating role for C / EBP gamma is promoter specific , neither being observed in the regulation of a simple C / EBP dependent promoter nor the TNF alpha promoter . ^^^ C / EBP gamma activity also shows cell type specificity with no activity observed in a macrophage cell line . ^^^ |
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| Interacting proteins: P53567 and P49715 |
Pubmed |
SVM Score :0.0 |
| Although five C / EBP family proteins are known which bind the E site , antibody ablation of DNA : protein complexes resolved by electrophoretic mobility shift assays showed that Ig / EBP 1 is the only E site binding protein detectable in early B cell lines ; more mature B cells contain Ig / EBP 1 and NF IL 6 . ^^^ |
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| Interacting proteins: P53567 and P49715 |
Pubmed |
SVM Score :0.0 |
| Ig / EBP 1 : a ubiquitously expressed immunoglobulin enhancer binding protein that is similar to C / EBP and heterodimerizes with C / EBP . ^^^ Among known family members , Ig / EBP 1 demonstrates highest homology to C / EBP throughout the DNA binding domain and leucine repeat region . ^^^ Ig / EBP 1 and C / EBP have highly overlapping binding specificities ; both cloned proteins bind to the IgH enhancer and the VH 1 promoter E sites , and Ig / EBP 1 binds to previously characterized C / EBP binding sites in the Rous sarcoma virus ( RSV ) LTR and the murine albumin promoter . ^^^ Consistent with their homology in the leucine repeat region , Ig / EBP 1 and C / EBP form heterodimers ; Ig / EBP 1 is the first member of this family that has been found to heterodimerize with the well characterized C / EBP . ^^^ In tissues where Ig / EBP 1 and C / EBP are both present , heterodimers may be functionally important . ^^^ |
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| Interacting proteins: P53567 and P49715 |
Pubmed |
SVM Score :0.0 |
| The C / EBP motif is bound by Ig / EBP 1 in pre B cell and T cell extracts , but is replaced by nuclear factor IL 6beta or a nuclear factor IL 6beta Ig / EBP 1 heterodimer in plasmacytoma cell extracts . ^^^ |
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| Interacting proteins: P53567 and P49715 |
Pubmed |
SVM Score :0.0 |
| The first interaction ( detailed here ) is between JDP 2 , a member of the basic leucine zipper ( bZIP ) family , and C / EBPgamma , a member of the CCAAT / enhancer binding protein ( C / EBP ) family . ^^^ |
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| Interacting proteins: P53567 and P49715 |
Pubmed |
SVM Score :0.0 |
| Regulation of CCAAT / enhancer binding protein ( C / EBP ) activator proteins by heterodimerization with C / EBPgamma ( Ig / EBP ) . ^^^ Biochemical fractionation and antibody supershift assays demonstrate that the C / EBPbeta heterodimeric partner is C / EBPgamma ( Ig / EBP ) , a C / EBP protein that has been implicated as an inhibitor of other family members . ^^^ Repression by C / EBPgamma occurs at the level of transactivation and requires heterodimerization with the C / EBP partner . ^^^ C / EBPgamma was an ineffective repressor in HepG 2 hepatoma cells despite forming C / EBP heterodimers , and C / EBPalpha was not effectively inhibited in either L or HepG 2 cells . ^^^ Our findings demonstrate that C / EBPgamma modulates C / EBP activity in a cell and isoform specific manner . . ^^^ |
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| Interacting proteins: P53567 and P49715 |
Pubmed |
SVM Score :0.0 |
| Here we show that inhibitory / regulatory C / EBP family proteins , Ig / EBP ( C / EBPgamma ) and CHOP ( C / EBPzeta ) , but not positively functioning NF IL 6 ( C / EBPbeta ) , are constitutively multiubiquitinated and subsequently degraded by the proteasome . ^^^ |
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| Interacting proteins: P53567 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P53567 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P53567 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P53567 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P53567 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P53567 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P53567 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P53567 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P53567 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P53567 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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