| Interacting proteins: Q99593 and P52952 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99593 and P52952 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q99593 and P52952 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q99593 and P52952 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q99593 and P52952 |
Pubmed |
SVM Score :0.0 |
| Here we show that , in Xenopus embryos , Tbx 5 is expressed in the early heart field , posterior to the cardiac homeobox transcription factor , Nkx2 . 5 . ^^^ |
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| Interacting proteins: Q99593 and P52952 |
Pubmed |
SVM Score :0.0 |
| Based on these findings and recent studies showing that haploinsufficiency for other cardiac transcription factor genes ( e . g . , TBX 5 , NKX 2 5 ) causes congenital heart disease , we postulate that GATA 4 deficiency may contribute to the phenotype of patients with monosomy of 8p23 . 1 . . ^^^ |
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| Interacting proteins: Q99593 and P52952 |
Pubmed |
SVM Score :0.0 |
| In chicken and mouse embryos , tbx 5 expression is initiated at the earliest stages of heart formation throughout the heart primordia and is colocalized with other cardiac transcription factors such as nkx 2 . 5 and GATA 4 . ^^^ |
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| Interacting proteins: Q99593 and P52952 |
Pubmed |
SVM Score :0.0 |
| Mutations of the TBX 5 , NKX2 . 5 , EVC , and PRKAR 1 alpha genes all result in abnormalities of human atrial growth and development , and mutations in at least one gene results in familial atrial fibrillation and is as yet unidentified . ^^^ |
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| Interacting proteins: Q99593 and P52952 |
Pubmed |
SVM Score :0.0 |
| Direct and cooperative transactivation of the ANF and cx 40 promoters by Tbx 5 and the homeodomain transcription factor Nkx 2 5 was also demonstrated . ^^^ |
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| Interacting proteins: Q99593 and P52952 |
Pubmed |
SVM Score :0.0 |
| Comparison of the 5 ' flanking regions of the human and murine corin genes revealed several conserved sequences , including binding sites for TBX 5 , GATA , NKX2 . 5 , and Krppel like transcription factors . ^^^ |
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| Interacting proteins: Q99593 and P52952 |
Pubmed |
SVM Score :0.0 |
| The TBX 5 gene , which encodes another cardiac transcription factor that collaborates with NKX2 . 5 , is also an important cardiac disease gene , with heterozygous mutations responsible for Holt Oram ( hand / heart ) syndrome . ^^^ |
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| Interacting proteins: Q99593 and P52952 |
Pubmed |
SVM Score :0.0 |
| Furthermore , we report that human ANF promoter is synergistically activated by Tbx 5 , Nkx2 . 5 and GATA 4 . ^^^ |
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| Interacting proteins: Q99593 and P52952 |
Pubmed |
SVM Score :0.0 |
| METHODS : We studied in vitro biochemical characteristics , including nuclear localization , DNA binding , transcriptional activation and protein protein interaction with transcriptional partners ( GATA 4 , TBX 5 and NKX2 . 5 itself ) , of eight homeodomain missense mutations . ^^^ |
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| Interacting proteins: Q99593 and P52952 |
Pubmed |
SVM Score :0.0 |
| Transcription factors representative of multiple subfamilies have been identified and include members of zinc finger subfamilies ( GATA 4 , GATA 6 HF 1b ) , skeletal muscle transcription factors ( MyoD ) , T box genes ( Tbx 5 ) , and also homeodomain transcription factors ( Msx 2 and Nkx2 . 5 ) . ^^^ |
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| Interacting proteins: Q99593 and P52952 |
Pubmed |
SVM Score :0.0 |
| Mouse models also addressed the role and contribution of specific signaling molecules , such as PRKAG 2 , Nkx2 . 5 , ALK 3 , and Tbx 5 , in the development of the cardiac conduction system and the pathogenesis of cardiac dysfunction in humans . . ^^^ |
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| Interacting proteins: Q99593 and P52952 |
Pubmed |
SVM Score :0.0 |
| Normal heart development is orchestrated by a set of highly conserved transcription factors that includes GATA 4 , Nkx 2 5 , and Tbx 5 . ^^^ In mouse models , haploinsufficiency for Nkx 2 5 or Tbx 5 resulted in an increased incidence of structural heart disease , confirming that normal heart development is sensitive to small changes in expression levels of Nkx 2 5 and Tbx 5 . ^^^ |
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| Interacting proteins: Q99593 and P52952 |
Pubmed |
SVM Score :0.0 |
| Transcriptional regulation of the murine Connexin 40 promoter by cardiac factors Nkx 2 5 , GATA 4 and Tbx 5 . ^^^ Several putative regulatory sites for transcription factors were predicted within this region by computer analysis , and we demonstrated that the nuclear factors Sp 1 , Nkx 2 5 , GATA 4 and Tbx 5 could interact specifically with elements present in the minimal promoter region of the Cx 40 . ^^^ Furthermore , co transfection experiments showed the ability of Nkx 2 5 and GATA 4 to transactivate the minimal Cx 40 promoter while Tbx 5 repressed Nkx 2 5 / GATA4 mediated activation . ^^^ CONCLUSION : In this work , we cloned the promoter region of the Cx 40 and demonstrated that the core promoter was modulated by cardiac transcriptional factors Nkx 2 5 , Tbx 5 and GATA 4 acting together with ubiquitous Sp1 . . ^^^ |
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| Interacting proteins: Q99593 and P52952 |
Pubmed |
SVM Score :0.0 |
| The expression of human NKX2 . 5 in C2C12 myoblasts inhibited myocyte differentiation and myotube formation , and up regulated Gata 4 and Tbx 5 expression . ^^^ |
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| Interacting proteins: Q99593 and P52952 |
Pubmed |
SVM Score :0.0 |
| Furthermore , we demonstrate that overexpression of Lbh in cultured mammalian cells represses the synergistic activity of key cardiac transcription factors , Nkx2 . 5 and Tbx 5 , leading to reduced activation of the common target gene , Anf ( Nppa ) . ^^^ Thus , repression of Nkx2 . 5 and Tbx 5 mediated gene expression by deregulated Lbh may account in part for the cardiac anomalies observed in these mice . ^^^ |
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| Interacting proteins: Q99593 and P52952 |
Pubmed |
SVM Score :0.0 |
| Cardiac transcription factors including Nkx2 . 5 and Tbx 5 mRNA were first expressed at day 4 in hanging drop embryoid bodies , and adhesion of embryoid bodies to surfaces at or before that day strongly inhibited differentiation of ES cells to cardiomyocytes . ^^^ |
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| Interacting proteins: Q99593 and P52952 |
Pubmed |
SVM Score :0.0 |
| Moreover , expression patterns of known key regulatory genes of atrial septum development , including Nkx2 . 5 , Pitx 2 , and Tbx 5 , are unaltered in the developing heart in Odd 1 ( / ) mutants compared to that of the wild type littermates . ^^^ |
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| Interacting proteins: Q99593 and P52952 |
Pubmed |
SVM Score :0.0 |
| Mutations in 5 human genes ( NKX2 . 5 , TBX 5 , GATA 4 , MYHC , ACTC ) are known to cause dominant ASD , but these account for a minority of cases . ^^^ |
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| Interacting proteins: Q99593 and P52952 |
Pubmed |
SVM Score :0.0 |
| Semiquantitative reverse transcription polymerase chain reaction analyses demonstrated expression of marker genes typical for endoderm , mesoderm , cardiac mesoderm , and CMs , including brachyury , goosecoid , Tbx 5 , Tbx 20 , Mesp 1 , Nkx2 . 5 , GATA 4 , FOG 2 , Mlc2a , MLC2v , ANF , and alpha MHC in rESC derived CMs . ^^^ |
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| Interacting proteins: Q99593 and P52952 |
Pubmed |
SVM Score :0.0 |
| P 19 murine embryonal carcinoma cells to beating cardiac myocytes : its expression is activated by the cardiac Gata 4 , Nkx2 . 5 , and Tbx 5 proteins . ^^^ Experiments with reporter constructs , including regulatory regions from the Ifi 204 gene ( encoding p 204 ) in P 19 cells and in cultured newborn rat cardiac myocytes , as well as chromatin coimmunoprecipitations with transcription factors , revealed that p 204 expression was synergistically transactivated by the cardiac Gata 4 , Nkx2 . 5 , and Tbx 5 transcription factors . ^^^ |
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| Interacting proteins: Q99593 and P52952 |
Pubmed |
SVM Score :0.0 |
| Chem . 281 , 14882 14892 ) that ( 1 ) the p 204 protein is required for the differentiation of murine P 19 embryonal carcinoma stem cells to beating cardiac myocytes , and ( 2 ) the expression of p 204 in the differentiating P 19 cells is synergistically transactivated by the cardiac transcription factors Gata 4 , Nkx2 . 5 , and Tbx 5 . ^^^ |
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| Interacting proteins: Q99593 and P52952 |
Pubmed |
SVM Score :0.0 |
| Vertebrate heart development involves many transcription factors such as Nkx2 . 5 , GATA , and tbx 5 . ^^^ |
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| Interacting proteins: Q99593 and P52952 |
Pubmed |
SVM Score :0.61320347 |
| All seven missense mutations greatly reduced the interaction of TBX 5 with NKX2 . 5 in vivo and in vitro . 0.61320347^^^ |
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| Interacting proteins: Q99593 and P52952 |
Pubmed |
SVM Score :0.58672823 |
| Mouse knockout and mammalian cell culture studies have shown that the ANF gene is regulated by combinatorial interactions between Nkx 2 5 , GATA 4 , Tbx 5 , and SRF ; however , the molecular mechanisms leading to chamber specific expression are currently unknown . 0.58672823^^^ |
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