| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.74290181 |
| Mouse knockout and mammalian cell culture studies have shown that the ANF gene is regulated by combinatorial interactions between Nkx 2 5 , GATA 4 , Tbx 5 , and SRF ; however , the molecular mechanisms leading to chamber specific expression are currently unknown . 0.74290181^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| The cardiac tissue restricted homeobox protein Csx / Nkx2 . 5 physically associates with the zinc finger protein GATA 4 and cooperatively activates atrial natriuretic factor gene expression . ^^^ In this study , we tested whether Csx and GATA 4 physically associate and cooperatively activate transcription of a target gene . ^^^ Coimmunoprecipitation experiments demonstrate that Csx and GATA 4 associate intracellularly . ^^^ Interestingly , in vitro protein protein interaction studies indicate that helix 3 of the homeodomain of Csx is required to interact with GATA 4 and that the carboxy terminal zinc finger of GATA 4 is necessary to associate with Csx . ^^^ The promoter enhancer region of the atrial natriuretic factor ( ANF ) contains several putative Csx binding sites and consensus GATA 4 binding sites . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| With a cardiac zinc finger protein GATA 4 , Csx induces cardiomyocyte differentiation of teratocarcinoma cells as well as upregulation of cardiac genes . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Novel cardiac regulatory factors such as Nkx 2 . 5 and GATA 4 , in addition to MEF 2 isoforms , are also beginning to be identified . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| A few transcription factors , among which Nkx 2 . 5 and GATA 4 , are expressed predominantly in the heart . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| The cardiac transcription factors Nkx 2 5 and GATA 4 are mutual cofactors . ^^^ The tissue restricted GATA 4 transcription factor and Nkx 2 5 homeodomain protein are two early markers of precardiac cells . ^^^ Overexpression of GATA 4 or Nkx 2 5 enhances cardiac development in committed precursors , suggesting each interacts with a cardiac cofactor . ^^^ We tested whether GATA 4 and Nkx 2 5 are cofactors for each other by using transcription and binding assays with the cardiac atrial natriuretic factor ( ANF ) promoter _ the only known target for Nkx 2 5 . ^^^ Co expression of GATA 4 and Nkx 2 5 resulted in synergistic activation of the ANF promoter in heterologous cells . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| BMP 2 was able to induce Nkx 2 5 expression ectopically within the anterior head domain , while GATA 4 was also induced more caudally . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| GATA 4 and Nkx 2 . 5 coactivate Nkx 2 DNA binding targets : role for regulating early cardiac gene expression . ^^^ We demonstrate here that Nkx 2 . 5 also cooperates with GATA 4 , a dual C 4 zinc finger transcription factor expressed in early cardiac progenitor cells , to activate the alphaCA promoter and a minimal promoter , containing only multimerized Nkx 2 . 5 DNA binding sites ( NKEs ) , in heterologous CV 1 fibroblasts . ^^^ Transcriptional activity requires the N terminal activation domain of Nkx 2 . 5 and Nkx 2 . 5 binding activity through its homeodomain but does not require GATA 4 ' s activation domain . ^^^ The minimal interactive regions were mapped to the homeodomain of Nkx 2 . 5 and the second zinc finger of GATA 4 . ^^^ Removal of Nkx 2 . 5 ' s C terminal inhibitory domain stimulated robust transcriptional activity , comparable to the effects of GATA 4 on wild type Nkx 2 . 5 , which in part facilitated Nkx 2 . 5 DNA binding activity . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| The basal cardiac promoter is the target of two cardiac specific transcription factors , the zinc finger GATA 4 protein and the Nkx 2 5 homeodomain , which bind to contiguous elements within this region . ^^^ This functional synergy requires physical interaction between the GATA 4 protein and an extended C terminal homeodomain on Nkx 2 5 . ^^^ Optimal synergy requires binding of both proteins to their cognate sites , although modest synergy also could be observed on heterologous promoters containing only multimerized Nkx binding sites , suggesting that Nkx 2 5 is able to recruit GATA 4 into a transcriptionally active complex . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| The GATA element of the slow MyHC 3 is sufficient for expression of a heterologous reporter gene in both atrial and ventricular cardiomyocytes , and expression of GATA 4 , but not Nkx 2 5 or myocyte enhancer factor 2C , activates reporter gene expression in fibroblasts . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Similar to Nkx 2 5 , MEF2C expression initiated cardiomyogenesis , resulting in the up regulation of Brachyury T , bone morphogenetic protein 4 , Nkx 2 5 , GATA 4 , cardiac alpha actin , and myosin heavy chain expression . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| In addition , potential autoregulatory elements can be cooperatively stimulated by Nkx 2 5 and GATA 4 . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Based on these findings and recent studies showing that haploinsufficiency for other cardiac transcription factor genes ( e . g . , TBX 5 , NKX 2 5 ) causes congenital heart disease , we postulate that GATA 4 deficiency may contribute to the phenotype of patients with monosomy of 8p23 . 1 . . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Transcripts for flk 1 , Nkx 2 . 5 , GATA 4 or smooth muscle markers were undetectable , indicating an absence of endothelial , myocardial or preformed smooth muscle cells . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Characterization of the murine A 1 adenosine receptor promoter , potent regulation by GATA 4 and Nkx2 . 5 . ^^^ Co expression studies revealed that GATA 4 and Nkx2 . 5 could individually drive A1AR promoter activity and act synergistically to activate A1AR expression . ^^^ These observations suggest that embryonic A1AR expression involves activation of the A1AR promoter by GATA 4 and Nkx2 . 5 . . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Here , we used a panel of marker genes with early expression within the germ layers ( oct 3 , Brachyury T , Fgf 5 , nodal , and GATA 4 ) or a variety of lineages ( flk 1 , Nkx 2 . 5 , EKLF , and Msx 3 ) to determine how progressive differentiation of embryoid bodies in culture correlated with early postimplantation development of mouse embryos . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Expression of immediate early genes , GATA 4 , and Nkx 2 . 5 in adrenergic induced cardiac hypertrophy and during regression in adult mice . ^^^ GATA 4 , Nkx 2 . 5 , Egr 1 , c jun and c fos expression were increased after 3 , 7 and 14 days of treatment . ^^^ After PE or ISO drug withdrawal the HW / BW was normal and Egr 1 , c jun , c fos and GATA 4 , but not Nkx2 . 5 , expression dropped to control levels . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| In addition , we have identified an essential GATA 4 binding site in the proximal upstream regulatory region of the gene and cooperative transcriptional regulation mediated by Nkx2 . 5 and GATA 4 . ^^^ We have shown that this cooperative regulation is dependent on binding of GATA 4 to its cognate DNA sequence in the promoter , which suggests that Nkx2 . 5 controls CARP expression , at least in part , through GATA 4 . . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| A role for GATA 4 / 5 / 6 in the regulation of Nkx2 . 5 expression with implications for patterning of the precardiac field . ^^^ In contrast , RA does not block expression of the GATA 4 / 5 / 6 genes , which are transcribed normally in an overlapping pattern with Nkx2 . 5 throughout cardiogenesis . ^^^ GATA dependent regulatory sequences of the Nkx2 . 5 gene that implicate GATA 4 / 5 / 6 as upstream positive regulators were described recently . ^^^ Regulatory mechanisms altered by excess RA must function normally to limit GATA 4 / 5 / 6 expression levels , to define the region of Nkx2 . 5 expression and regulate myocardial differentiation . . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Vertebrate heart development is initiated from bilateral lateral plate mesoderm that expresses the Nkx2 . 5 and GATA 4 transcription factors , but the extracellular signals specifying heart precursor gene expression are not known . ^^^ Fgf 8 is required for the earliest stages of nkx2 . 5 and gata 4 , but not gata 6 , expression in cardiac precursors . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| The zinc finger transcription factors GATA 4 , 5 , and 6 and the homeodomain protein Nkx2 . 5 are expressed in the developing heart and have been shown to activate a variety of cardiac specific genes . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| In chicken and mouse embryos , tbx 5 expression is initiated at the earliest stages of heart formation throughout the heart primordia and is colocalized with other cardiac transcription factors such as nkx 2 . 5 and GATA 4 . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Zinc finger containing transcription factor GATA 4 and homeodomain Nkx 2 . 5 govern crucial developmental fates and have been found to promote cardiogenesis in embryonic cells exposed to the differentiating agent DMSO . ^^^ DMSO primed GATA 4 and Nkx 2 . 5 gene expression was preceded by a marked increase in prodynorphin gene expression and dynorphin B synthesis and secretion . ^^^ In the absence of DMSO , dynorphin B triggered GATA 4 and Nkx 2 . 5 gene expression and led to the appearance of both alpha myosin heavy chain and myosin light chain 2V transcripts , two markers of cardiac differentiation . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| The development of transplanted and cultured cells in cardiomyocytes was evaluated by testing the expression of several cardiac transcription factor genes ( Nkx 2 . 5 , eHAND , dHAND , GATA 4 ) , alpha cardiac actin mRNA , and beta myosin heavy chain protein . ^^^ In vitro analyses showed that cells with the potential to form cardiac muscle were present prior to gastrulation in 6 . 5 day postconception ( dpc ) epiblasts , as indicated by the expression of Nkx 2 . 5 , eHAND , dHAND , and GATA 4 cardiac transcription factors ; desmin transgene ; alpha cardiac actin ; and beta myosin heavy chain . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| During each embryogenesis , the beta MHC gene is expressed as part of the cardiac myogenic program under the control of NKX 2 . 5 , MEF 2C , and GATA 4 / 5 / 6 . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Cells in the secondary heart field express Nkx2 . 5 and Gata 4 , as do the cells of the primary heart fields . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| The timing and regulation of zebrafish hrT expression in anterior lateral plate mesoderm suggest a very early role for hrT in the differentiation of the cardiac precursors . hrT is coexpressed with gata 4 and nkx2 . 5 not only in anterior lateral plate mesoderm but also in noncardiac mesoderm adjacent to the tail bud , suggesting that a conserved regulatory pathway links expression of these three genes in cardiac and noncardiac tissues . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Addition of Noggin to explants isolated at stage 4 and cultured for 24 h resulted in loss of NKX2 . 5 , GATA 4 , eHAND , Mef2A and vMHC expression . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Unmanipulated early growth response 1 ( Egr 1 ) deficient / mice have similar heart to body weight ratios but express lower amounts of atrial natriuretic factor ( ANF ) , beta myosin heavy chain ( beta MHC ) , skeletal actin , NGF 1 A binding protein ( NAB ) 2 , Sp 1 , c fos , c jun , GATA 4 , and Nkx2 . 5 than + / + or + / mice . alpha MHC , tubulin , and NAB 1 expression was similar . ^^^ Only Iso + PE treated + / + mice showed induction of NAB 1 , GATA 4 , and Nkx2 . 5 . ^^^ Surprisingly , vehicle treated / mice displayed fibrosis and increased Sp 1 , skeletal actin , Nkx2 . 5 , and GATA 4 expression without hypertrophy . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| The zinc finger containing transcription factor GATA 4 , which is widely expressed in mesodermal and endodermal derived tissues , is thought to regulate cardiac myocyte specific gene expression through combinatorial interactions with other semi restricted transcription factors such as myocyte enhancer factor 2 , nuclear factor of activated T cells , serum response factor , and Nkx2 . 5 . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Examination of markers of precardiac tissue showed that expression of GATA 4 is normal , while the levels of Nkx 2 5 mRNA are strongly reduced . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| The cells expressed Nkx2 . 5 , GATA 4 , TEF 1 , and MEF 2 C mRNA before 5 azacytidine exposure , and MEF 2 A and MEF 2 D after exposure . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| The CLP 1 gene promoter is active in different cell types and sequence analysis shows a number of potential binding sites for cardiogenic transcription factors such as Nkx2 . 5 and GATA 4 , indicating a potential role in development . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| The beating cells expressed markers characteristic of cardiomyocytes , such as cardiac alpha myosin heavy chain , cardiac troponin 1 and T , atrial natriuretic factor , and cardiac transcription factors GATA 4 , Nkx2 . 5 , and MEF 2 . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Cardiac muscle specific members of the NK and GATA families exist , and the triad of Nkx 2 . 5 , serum response factor , and GATA 4 also transactivated the cardiac atrial natriuretic factor gene , which contains a CArG like box , a GATA binding box , and an NK binding element . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Combinatorial expression of GATA 4 , Nkx 2 5 , and serum response factor directs early cardiac gene activity . ^^^ Herein , the restricted expression of serum response factors ( SRF ) closely overlapped with Nkx 2 5 and GATA 4 transcripts in early chick embryos coinciding with the earliest appearance of cardiac alpha actin ( alphaCA ) transcripts and nascent myocardial cells . ^^^ The combinatorial expression of SRF , a MADS box factor Nkx 2 5 ( a NK 4 homeodomain ) , and / or GATA 4 , a dual C 4 zinc finger protein , in heterologous CV 1 fibroblasts and Schneider 2 insect cells demonstrated synergistic induction of alphaCA promoter activity . ^^^ These assays indicated a dependence upon cooperative SRF binding for facilitating the recruitment of Nkx 2 5 and GATA 4 to the alphaCA promoter . ^^^ Furthermore , the recruitment of Nkx 2 5 and GATA 4 by SRF was observed to strongly enhance SRF DNA binding affinity . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| After binding to their receptor , growth factors induce expression of a number of cardiac specific transcription factors such as the zinc finger GATA proteins and Nkx 2 . 5 , a coactivator of the GATA 4 protein . ^^^ The GATA 4 transcription factor and Nkx 2 . 5 are essential for heart development . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| We investigated the effects of Aroclor 1254 , a complex mixture of > 80 PCB isomers and congeners , on the expression of nuclear transcription factors ( GATA 4 , Nkx 2 . 5 , MEF 2c , OCT 1 ) and of downstream target genes ( atrial and brain natriuretic peptide , alpha and beta myosin heavy chain , alpha cardiac and alpha skeletal actin ) , which play an important role in cardiac biology . 3 . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| The development of cultured and transplanted cells in cardiomyocytes was evaluated by testing the expression of several cardiac transcription factor genes ( Nkx 2 . 5 , eHAND , dHAND , GATA 4 ) , alpha cardiac actin , and beta myosin heavy chain protein . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Cardiac T box factor Tbx 20 directly interacts with Nkx 2 5 , GATA 4 , and GATA 5 in regulation of gene expression in the developing heart . ^^^ Tbx 20 physically interacted with cardiac transcription factors Nkx 2 5 , GATA 4 , and GATA 5 , collaborating to synergistically activate cardiac gene expression . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Furthermore , we report that human ANF promoter is synergistically activated by Tbx 5 , Nkx2 . 5 and GATA 4 . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| In all cases , the manipulations that restricted the size of the myocardium also restricted the expression of Nkx2 . 5 and GATA 4 , both key regulatory genes in the cardiogenic pathway . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| RT PCR analyses showed that the transcription factors Nkx2 . 5 , Gata 4 , Mef2c and Irx 4 were expressed at levels in the same order of magnitude as the levels observed in embryonic , neonatal and adult hearts . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| The prodynorphin gene and its product , dynorphin B , have been found to promote cardiogenesis in embryonic cells by inducing the expression of GATA 4 and Nkx 2 . 5 , two transcription factor encoding genes essential for cardiogenesis . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Direct exposure of nuclei isolated from undifferentiated ES cells to dynorphin B or U 50 , 488H , a synthetic kappa opioid receptor agonist , time and dose dependently activated the transcription of GATA 4 and Nkx 2 . 5 , 2 cardiac lineage promoting genes . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| These results show that activation of GATA 4 , in cooperation with a factor binding on Nkx 2 . 5 binding element , is essential for mechanical stretch induced cardiomyocyte hypertrophy . . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| GATA 4 and Nkx 2 . 5 seem to be essential for heart development . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| The different cardiac expression of the type 2 iodothyronine deiodinase gene between human and rat is related to the differential response of the Dio 2 genes to Nkx 2 . 5 and GATA 4 transcription factors . ^^^ Here we report that the human Dio 2 promoter is very sensitive to the cardiac transcription factors Nkx 2 . 5 and GATA 4 . ^^^ In coexpression studies , GATA 4 alone was a poor inducer of the hDio 2 promoter ; however in synergy with Nkx 2 . 5 , it activated D 2 reporter gene expression in the human , but not the rat promoter . ^^^ Functional analysis showed that both C and D sites are required for the complete Nkx 2 . 5 response and for the Nkx 2 . 5 / GATA 4 synergistic effect . ^^^ In conclusion , this study shows that Nkx 2 . 5 and GATA 4 play prime roles in Dio 2 gene regulation in the human heart and suggests that it is their synergistic action in humans that causes the differential expression of the cardiac Dio 2 gene between humans and rats . . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Transcriptional regulation of the murine Connexin 40 promoter by cardiac factors Nkx 2 5 , GATA 4 and Tbx 5 . ^^^ Several putative regulatory sites for transcription factors were predicted within this region by computer analysis , and we demonstrated that the nuclear factors Sp 1 , Nkx 2 5 , GATA 4 and Tbx 5 could interact specifically with elements present in the minimal promoter region of the Cx 40 . ^^^ Furthermore , co transfection experiments showed the ability of Nkx 2 5 and GATA 4 to transactivate the minimal Cx 40 promoter while Tbx 5 repressed Nkx 2 5 / GATA4 mediated activation . ^^^ CONCLUSION : In this work , we cloned the promoter region of the Cx 40 and demonstrated that the core promoter was modulated by cardiac transcriptional factors Nkx 2 5 , Tbx 5 and GATA 4 acting together with ubiquitous Sp1 . . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Here , we show that JMJ represses ANF gene expression by inhibiting transcriptional activities of Nkx2 . 5 and GATA 4 . ^^^ JMJ represses the Nkx2 . 5 or GATA 4 dependent activation of the reporter genes containing the ANF promoter enhancer or containing the Nkx2 . 5 or GATA 4 binding consensus sequence . ^^^ JMJ physically associates with Nkx2 . 5 and GATA 4 in vitro and in vivo as determined by glutathione S transferase pull down and immunoprecipitation assays . ^^^ Using mutational analyses , we mapped the protein protein interaction domains in JMJ , Nkx2 . 5 , and GATA 4 . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| METHODS : We studied in vitro biochemical characteristics , including nuclear localization , DNA binding , transcriptional activation and protein protein interaction with transcriptional partners ( GATA 4 , TBX 5 and NKX2 . 5 itself ) , of eight homeodomain missense mutations . ^^^ |
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| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Transcription factors representative of multiple subfamilies have been identified and include members of zinc finger subfamilies ( GATA 4 , GATA 6 HF 1b ) , skeletal muscle transcription factors ( MyoD ) , T box genes ( Tbx 5 ) , and also homeodomain transcription factors ( Msx 2 and Nkx2 . 5 ) . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Normal heart development is orchestrated by a set of highly conserved transcription factors that includes GATA 4 , Nkx 2 5 , and Tbx 5 . ^^^ Surprisingly , expression levels of the putative GATA 4 target genes ANF , BNP , MEF2C , Nkx 2 5 , cyclin D 2 , and BMP 4 were unchanged in mutant hearts , suggesting that GATA 4 is not a dose limiting regulator of the expression of these genes during later stages of embryonic cardiac development . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| The dHAND basic helix loop helix ( bHLH ) transcription factor , which is essential for heart development and extra embryonic structures , is thought to regulate cardiomyocyte specific gene expression through combinatorial interactions with other cardiac restricted transcription factors such as GATA 4 and NKX2 . 5 . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Here , we show that exposure of mouse ES cells to extremely low frequency magnetic fields triggered the expression of GATA 4 and Nkx 2 . 5 , acting as cardiac lineage promoting genes in different animal species , including humans . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| TGF beta increased the cellular expression of myosin , troponins , connexin 43 , GATA 4 , and NKx 2 . 5 , which suggested that it induced the myogenic differentiation of CD117+ cells . ^^^ CONCLUSIONS : TGF beta conducted the myogenic differentiation of CD117+ stem cells by upregulating GATA 4 and NKx 2 . 5 expression . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Within 12 days of differentiation , these cells expressed markers characteristic of cardiomyocytes and hepatocytes such as GATA 4 , Nkx2 . 5 , beta myosin heavy chain , myosin light chain 2V , cardiac troponin T , sodium calcium exchanger and HNF 4alpha , alpha fetoprotein , albumin , transthyretin , and CK 18 , respectively . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| The expression of human NKX2 . 5 in C2C12 myoblasts inhibited myocyte differentiation and myotube formation , and up regulated Gata 4 and Tbx 5 expression . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| In response to 1 mM of 5 aza , P 19 cells expressed bone morphogenetic protein 2 ( BMP 2 ) , BMP 4 , Bmpr1a and Smad 1 at day 6 or 9 , and also cardiac markers such as GATA 4 , Nkx2 . 5 , cardiac troponin 1 , and desmin were up regulated in a time dependent manner after induction of BMP signaling molecules . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Spectrum of atrial septal defects associated with mutations of NKX2 . 5 and GATA 4 transcription factors . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Semiquantitative reverse transcription polymerase chain reaction analysis showed that cardiomyogenesis and CCS specific markers , such as Nkx2 . 5 , GATA 4 , Irx 4 , Connexin 40 , Connexin 45 , HF 1b , and MinK , were up regulated in the presence of either growth factor . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| However , Shh expression induced cardiomyogenesis following cellular aggregation , resulting in the expression of factors expressed in cardiac muscle including GATA 4 , MEF2C , and Nkx 2 5 . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Phenotypes with GATA 4 or NKX2 . 5 mutations in familial atrial septal defect . ^^^ Recently , GATA 4 and NKX2 . 5 were reported as the disease genes of atrial septal defect ( ASD ) but the relationship between the locations of their mutations and phenotypes is not clear . ^^^ We analyzed GATA 4 and NKX2 . 5 mutations in 16 familial ASD cases , including four probands with atrioventricular conduction disturbance ( AV block ) and two with pulmonary stenosis ( PS ) , by PCR and direct sequencing , and examined their phenotypes clinically . ^^^ Five mutations , including two GATA 4 and three NKX2 . 5 mutations , were identified in 31 . 3 % of the probands with ASD , and three of them were novel . ^^^ The two GATA 4 mutations in the probands without AV block were S52F and E359Xfs ( c . 1075delG ) that was reported previously , and three NKX2 . 5 mutations in the probands with AV block were A88Xfs ( c . 262delG ) , R190C , and T178M . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Tbx 20 synergized with Isl 1 and Gata 4 to activate both the Mef2c and Nkx 2 5 enhancers , thus providing a unifying mechanism for gene activation by Tbx 20 in the AHF . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Cardiomyocytes were evident as they expressed cardiac cell specific genes and protein markers including GATA 4 , Nkx2 . 5 , beta MHC , atrial natriuretic factor ( ANF ) and alpha actin when analyzed by reverse transcription polymerase chain reaction ( RT PCR ) and immunohistochemical staining . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Mutations in 5 human genes ( NKX2 . 5 , TBX 5 , GATA 4 , MYHC , ACTC ) are known to cause dominant ASD , but these account for a minority of cases . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| We observed the absence of myogenic alpha actins , SM22alpha , and myocardin expression and the failure to form beating cardiac myocytes in aggregated SRF null embryonic stem cells , whereas the appearance of transcription factors Nkx 2 5 and GATA 4 were unaffected . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| The isolated MSCs produced colonies with a fibroblast like morphology in 2 weeks ; over 95 % expressed CD 71 , and 28 % expressed the cardiomyocyte specific transcription factor , Nkx2 . 5 , as well as a skeletal actin , Nkx2 . 5 , and GATA 4 . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Members of the GATA 4 , 5 , and 6 subfamily of transcription factors are co expressed with the homeoprotein Nkx 2 . 5 in the precardiac mesoderm during the earliest stages of its specification and are known to be important determinants of cardiac gene expression . ^^^ Here we demonstrate that when P 19 CL6 cells are induced to differentiate to a cardiogenic lineage , the expression of GATA 4 and GATA 6 is up regulated prior to the transcriptional activation of Nkx 2 . 5 . ^^^ Moreover , over expression of GATA 4 or 6 at the time of Nkx 2 . 5 induction results in a significant up regulation of endogenous Nkx 2 . 5 transcription . ^^^ |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| The expression of developmental and functional cardiac markers , Nkx 2 . 5 , MLC2V , GATA 4 , Connexin 43 , Connexin 45 , Serca 2a , cardiac alpha actin , and phospholamban , among others , was confirmed in LMPC ES derived cardiomyocytes . ^^^ |
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| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| LIF stimulation resulted in the induction of endothelial cell specific genes , including VE cadherin , Flk 1 , and CD 31 , whereas neither smooth muscle nor cardiac muscle marker genes such as GATA 4 , GATA 6 , Nkx 2 . 5 , and calponin were up regulated . ^^^ |
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| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| MOMPs labeled with a fluorescent marker and co cultivated with rat cardiomyocytes for 4 weeks expressed the cardiomyocyte specific transcription factors Nkx2 . 5 , GATA 4 , eHAND , and MEF 2 and the hematopoietic / monocytic markers CD 45 and CD 14 within 10 days and retained their proliferative capacity for up to 16 days . ^^^ |
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| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Overexpression of hhLIM in P 19 cells can enhance expression of cardiac marker genes Nkx2 . 5 and GATA 4 and potentiate development of cardiomyocyte like morphology . ^^^ |
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| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Icariin significantly elevated mRNA levels of cardiac transcription factors GATA 4 and Nkx2 . 5 , and cardiac specific alpha MHC , MLC 2v and beta AR genes in a concentration and time dependent manner ( P < 0 . 05 ) . ^^^ |
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| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Semiquantitative reverse transcription polymerase chain reaction analyses demonstrated expression of marker genes typical for endoderm , mesoderm , cardiac mesoderm , and CMs , including brachyury , goosecoid , Tbx 5 , Tbx 20 , Mesp 1 , Nkx2 . 5 , GATA 4 , FOG 2 , Mlc2a , MLC2v , ANF , and alpha MHC in rESC derived CMs . ^^^ |
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| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| P 19 murine embryonal carcinoma cells to beating cardiac myocytes : its expression is activated by the cardiac Gata 4 , Nkx2 . 5 , and Tbx 5 proteins . ^^^ Experiments with reporter constructs , including regulatory regions from the Ifi 204 gene ( encoding p 204 ) in P 19 cells and in cultured newborn rat cardiac myocytes , as well as chromatin coimmunoprecipitations with transcription factors , revealed that p 204 expression was synergistically transactivated by the cardiac Gata 4 , Nkx2 . 5 , and Tbx 5 transcription factors . ^^^ Furthermore , ectopic p 204 triggered the expression of Gata 4 and Nkx2 . 5 in P 19 cells . p 204 contains a nuclear export signal and was partially translocated to the cytoplasm during the differentiation . p 204 from which the nuclear export signal was deleted was not translocated , and it did not induce differentiation . ^^^ |
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| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Chem . 281 , 14882 14892 ) that ( 1 ) the p 204 protein is required for the differentiation of murine P 19 embryonal carcinoma stem cells to beating cardiac myocytes , and ( 2 ) the expression of p 204 in the differentiating P 19 cells is synergistically transactivated by the cardiac transcription factors Gata 4 , Nkx2 . 5 , and Tbx 5 . ^^^ This was in consequence of the binding of Id 1 , Id 2 , or Id 3 protein to the Gata 4 and Nkx2 . 5 proteins and the resulting inhibitions ( 1 ) of the binding of these transcription factors to each other and to DNA and ( 2 ) of their synergistic transactivation of the expression of various genes , including atrial natriuretic factor and Ifi 204 ( encoding p 204 ) . p 204 overcame this inhibition by Id proteins in consequence of ( 1 ) binding and sequestering Id proteins , ( 2 ) accelerating their ubiquitination and degradation by proteasomes , and ( 3 ) decreasing the level of Id proteins in the nucleus by increasing their translocation from the nucleus to the cytoplasm . ^^^ In the course of the differentiation , Gata 4 , Nkx2 . 5 , and p 204 were components of a positive feedback loop . ^^^ This loop arose in consequence of it that p 204 overcame the inhibition of the synergistic activity of Gata 4 and Nkx2 . 5 by the Id proteins . . ^^^ |
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| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| These improvements in LV function were associated with 70 % more blood vessels and a doubling of cells expressing cardiomyocyte specific transcription factors GATA 4 , Nkx2 . 5 and alpha actinin cells within the infarct zone . ^^^ |
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| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Our data are consistent with the hypothesis that BMP dependent activation of transcription factors including GATA 4 , Nkx2 . 5 , and MEF 2C augments cardiac differentiation mediated by cooperative control of Smad and Wnt signaling pathways . ^^^ |
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| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Accordingly , no expression of human Nkx2 . 5 , GATA 4 , or cardiac troponin 1 was detected . ^^^ |
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| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| However , upon aggregation , these Oct 4 positive cells , termed P 19 SI because they `` self induce ' ' cardiac muscle , exhibit increased mRNAs encoding the mesodermal factor Brachyury , cardiac transcription factors Nkx 2 . 5 and GATA 4 , the transcriptional repressor Msx 1 , and cytokines Wnt 3a , Noggin , and BMP 4 . ^^^ |
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| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| Skeletal muscle derived cells expressed cardiac specific proteins such as cardiac troponin T and atrial natriuretic peptide as well as cardiac enriched transcription factors such as Nkx2E ( formerly called Csx / Nkx2 . 5 ) and GATA 4 by coculture with cardiomyocytes . ^^^ |
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| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52952 and P43694 |
Pubmed |
SVM Score :0.0 |
| NA |
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