| Interacting proteins: P52952 and P11831 |
Pubmed |
SVM Score :0.86914766 |
| Investigation of protein protein interactions showed that Nkx 2 . 5 was bound to SRF in the absence of DNA in soluble protein complexes retrieved from cardiac myocyte nuclei but could also be detected in coassociated binding complexes on the proximal SRE 1 . 0.86914766^^^ |
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| Interacting proteins: P52952 and P11831 |
Pubmed |
SVM Score :0.67205105 |
| Mouse knockout and mammalian cell culture studies have shown that the ANF gene is regulated by combinatorial interactions between Nkx 2 5 , GATA 4 , Tbx 5 , and SRF ; however , the molecular mechanisms leading to chamber specific expression are currently unknown . 0.67205105^^^ |
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| Interacting proteins: P52952 and P11831 |
Pubmed |
SVM Score :0.0 |
| Activation of the cardiac alpha actin promoter depends upon serum response factor , Tinman homologue , Nkx 2 . 5 , and intact serum response elements . ^^^ In this study , Nkx 2 . 5 facilitated serum response factor ( SRF ) DNA binding activity to the multiple SREs found on the cardiac alpha actin promoter and together stimulated cardiac alpha actin promoter dependent transcription in 10T1 / 2 fibroblasts . ^^^ Analysis of cardiac alpha actin promoter mutants demonstrated the importance of the multiple upstream SREs and an obligatory requirement for an intact proximal SRE 1 , for providing high levels of activity in the presence of Nkx 2 . 5 and SRF coexpression . ^^^ Transfection assays with mutant SRF species indicated that the C terminal activation domain and DNA binding MADS box were necessary for transcriptional activity in the presence of Nkx 2 . 5 . ^^^ Expression of Nkx 2 . 5 mutants also demonstrated that the homeodomain alone was insufficient for directing promoter activity in the presence of SRF . ^^^ |
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| Interacting proteins: P52952 and P11831 |
Pubmed |
SVM Score :0.0 |
| Competition between negative acting YY 1 versus positive acting serum response factor and tinman homologue Nkx 2 . 5 regulates cardiac alpha actin promoter activity . ^^^ We recently showed that the cardiogenic homeodomain factor , Nkx 2 . 5 , served as a positive acting accessory factor for serum response factor ( SRF ) and together provided strong transcriptional activation of the cardiac alpha actin promoter . ^^^ In addition , Nkx 2 . 5 and SRF collaborated to activate the endogenous murine cardiac alpha actin gene in 10T1 / 2 fibroblasts , by a mechanism that involved coassociation of SRF and Nkx 2 . 5 on intact SREs of the alpha actin promoter . ^^^ Here , we show that the second SRE of the avian cardiac alpha actin promoter served as a binding site for Nkx 2 . 5 , SRF , and zinc finger containing GLI Kruppel like factor , YY 1 . ^^^ Expression of YY 1 inhibited cardiac alpha actin promoter activity , whereas coexpression of Nkx 2 . 5 and SRF was able to partially reverse YY 1 repression . ^^^ |
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| Interacting proteins: P52952 and P11831 |
Pubmed |
SVM Score :0.0 |
| The cardiogenic homeodomain factor Nkx 2 . 5 and serum response factor ( SRF ) provide strong transcriptional coactivation of the cardiac alpha actin ( alphaCA ) promoter in fibroblasts ( C . ^^^ Therefore , alphaCa promoter activity appears to be regulated through the combinatorial interactions of at least three cardiac tissue enriched transcription factors , Nkx 2 . 5 , GATA 4 , and SRF . . ^^^ |
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| Interacting proteins: P52952 and P11831 |
Pubmed |
SVM Score :0.0 |
| The presumed promoter sequence for CDV 1 located in the intron 14 of CDV 1R contained the common TATA box and consensus binding sites for various transcription factors ( Nkx 2 . 5 , Spl , C / EBP , SRF , YY 1 , and CREB ) , which seem to play roles in the heart specific expression and carnitine deficiency associated suppression of CDV 1 . ^^^ |
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| Interacting proteins: P52952 and P11831 |
Pubmed |
SVM Score :0.0 |
| Cardiac muscle specific members of the NK and GATA families exist , and the triad of Nkx 2 . 5 , serum response factor , and GATA 4 also transactivated the cardiac atrial natriuretic factor gene , which contains a CArG like box , a GATA binding box , and an NK binding element . ^^^ |
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| Interacting proteins: P52952 and P11831 |
Pubmed |
SVM Score :0.0 |
| Combinatorial expression of GATA 4 , Nkx 2 5 , and serum response factor directs early cardiac gene activity . ^^^ Herein , the restricted expression of serum response factors ( SRF ) closely overlapped with Nkx 2 5 and GATA 4 transcripts in early chick embryos coinciding with the earliest appearance of cardiac alpha actin ( alphaCA ) transcripts and nascent myocardial cells . ^^^ The combinatorial expression of SRF , a MADS box factor Nkx 2 5 ( a NK 4 homeodomain ) , and / or GATA 4 , a dual C 4 zinc finger protein , in heterologous CV 1 fibroblasts and Schneider 2 insect cells demonstrated synergistic induction of alphaCA promoter activity . ^^^ These assays indicated a dependence upon cooperative SRF binding for facilitating the recruitment of Nkx 2 5 and GATA 4 to the alphaCA promoter . ^^^ Furthermore , the recruitment of Nkx 2 5 and GATA 4 by SRF was observed to strongly enhance SRF DNA binding affinity . ^^^ |
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| Interacting proteins: P52952 and P11831 |
Pubmed |
SVM Score :0.0 |
| The zinc finger containing transcription factor GATA 4 , which is widely expressed in mesodermal and endodermal derived tissues , is thought to regulate cardiac myocyte specific gene expression through combinatorial interactions with other semi restricted transcription factors such as myocyte enhancer factor 2 , nuclear factor of activated T cells , serum response factor , and Nkx2 . 5 . ^^^ |
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| Interacting proteins: P52952 and P11831 |
Pubmed |
SVM Score :0.0 |
| Differential regulation of the cardiac sodium calcium exchanger promoter in adult and neonatal cardiomyocytes by Nkx2 . 5 and serum response factor . ^^^ Nkx2 . 5 and serum response factor ( SRF ) are critically important transcription factors in cardiac morphogenesis . ^^^ In this paper we demonstrate that the interaction of Nkx2 . 5 and SRF in cardiac specific gene regulation is different between neonatal and adult cardiomyocytes . ^^^ In rat neonatal cardiomyocytes NCX 184 activity is substantially induced with Nkx2 . 5 or SRF and additively with both . ^^^ In contrast , in adult cardiomyocytes , co infection of Nkx2 . 5 and SRF adenovirus vectors showed Nkx2 . 5 induction but SRF did not have additive effects on NCX 1 promoter regulation . ^^^ |
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| Interacting proteins: P52952 and P11831 |
Pubmed |
SVM Score :0.0 |
| This protein interacted mainly with the transcriptional activation domain of the SRF protein and was found to bind to SRF or to the complex of SRF and another cofactor , such as myocardin or Nkx2 . 5 . ^^^ |
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| Interacting proteins: P52952 and P11831 |
Pubmed |
SVM Score :0.0 |
| We observed the absence of myogenic alpha actins , SM22alpha , and myocardin expression and the failure to form beating cardiac myocytes in aggregated SRF null embryonic stem cells , whereas the appearance of transcription factors Nkx 2 5 and GATA 4 were unaffected . ^^^ |
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| Interacting proteins: P52952 and P11831 |
Pubmed |
SVM Score :0.0 |
| The zipzap protein interacted with SRF in vivo and was found in protein complexes containing SRF and other SRF cofactors , including p49 / strap and Nkx2 . 5 . ^^^ |
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| Interacting proteins: P52952 and P11831 |
Pubmed |
SVM Score :0.0 |
| Like its cardiac homolog , NKX2 . 5 , NKX3 . 1 acts synergistically with serum response factor ( SRF ) to activate expression from the smooth muscle gamma actin ( SMGA ) gene promoter . ^^^ |
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| Interacting proteins: P52952 and P11831 |
Pubmed |
SVM Score :0.0 |
| Previous studies have shown that HOP , which consists entirely of a putative homeodomain , acts downstream of Nkx2 . 5 and associates with the serum response factor ( SRF ) , repressing transcription from SRF responsive genes . ^^^ |
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| Interacting proteins: P52952 and P11831 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52952 and P11831 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52952 and P11831 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52952 and P11831 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52952 and P11831 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52952 and P11831 |
Pubmed |
SVM Score :0.0 |
| NA |
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