| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| In both GSF and MCF , GLUT 2 was generally decreased or partially absent at all time points . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| We find that in addition to insulin the insulinoma express the normal beta cell markers Pdx 1 , IAPP , and Glut 2 , and that these markers are absent from the glucagonoma : a reflection of the normal alpha cell . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| The exogenous expression of PDX 1 in alphaTC1 . 6 cells alone could induce islet amyloid polypeptide ( IAPP ) mRNA expression in the cells but not the expression of insulin , glucokinase , or GLUT 2 gene . ^^^ GLUT 2 mRNA remained undetectable in the PDX 1 expressing alphaTC1 . 6 cells . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| The expression of other known beta cell specific factors such as Pax 4 , Pax 6 , Pdx 1 , GLUT 2 and GLP 1 R is unchanged by the introduction of Nkx6 . 1 . . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| The differential expression of the insulin , PDX 1 , GLUT 2 , and glucokinase genes were assessed by quantitative reverse transcription polymerase chain reaction . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| As glucose uptake / metabolism is essential for insulin secretion , decreased GLUT 2 may contribute to the etiology of diabetes in pdx 1 ( PB ) HNF 6 transgenics . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| Alternatively , beta cells have been suggested to arise late , directly from the GLUT 2 and pancreatic duodenal homeobox factor 1 ( PDX 1 ) expressing epithelium , which gives rise also to the acinar cells during this stage . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| Altered beta cell distribution of pdx 1 and GLUT 2 after a short term challenge with a high fat diet in C57BL / 6J mice . ^^^ However , the nuclear translocation of the homeobox transcription factor , pdx 1 , and the plasma membrane translocation of GLUT 2 were both impaired in high fat fed animals after 1 week . ^^^ The early onset islet dysfunction is accompanied by impaired beta cell trafficking of two factors , pdx 1 and GLUT 2 , which are involved in beta cell proliferation and glucose recognition . ^^^ |
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| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| The loss in beta cell function in Pdx 1 ( + / ) / Hnf 3beta ( + / ) mice was associated with decreased expression of Nkx 6 . 1 , glucokinase ( Gck ) , aldolase B ( aldo B ) , and insulin , whereas Nkx2 . 2 , Nkx 6 . 1 , Glut 2 , Gck , aldo B , the liver isoform of pyruvate kinase , and insulin expression was reduced in Pdx 1 ( + / ) / Hnf 1alpha ( + / ) mice . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| As the glucose transporter type 2 ( GLUT 2 ) gene expression is selectively decreased in the beta pancreatic cells of experimental models of diabetes , we postulated that the loss of GLUT 2 gene expression in the pancreatic islets of diabetic animals may be due to the loss of PDX 1 transacting function on the GLUT 2 gene . ^^^ We , therefore , investigated the potential role of PDX 1 in the transcriptional control of GLUT 2 . ^^^ These data demonstrate that the murine GLUT 2 promoter is controlled by the PDX 1 homeobox factor through the identified GLUT2TAAT motif . . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| In this report , we assessed the DNA binding activities of GTIIa and PDX 1 to their respective cis elements of the GLUT 2 promoter using nuclear extracts prepared from pancreatic islets of 12 week old db / db diabetic mice . ^^^ We show that the decreased GLUT 2 mRNA expression correlates with a decrease of the GTIIa DNA binding activity , whereas the PDX 1 binding activity is increased . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| The noninsulinomas are often periductal in location , express none of the four major islet peptide hormones , Glut 2 , Pdx 1 , tyrosine hydroxylase , Pax 4 , Pax 6 , or Nkx6 . 1 , but do express glucokinase , Sur 1 , Isl 1 , Hnf3beta , Hnf 6 , Beta2 / NeuroD , and Nkx2 . 2 . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| GLUT 2 mRNA was decreased , but other beta cell associated genes ( IAPP [ islet amyloid pancreatic polypeptide ] , PDX 1 [ pancreatic and duodenal homeobox 1 ] , and BETA2 / NeuroD ) were expressed at near normal levels . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| They were found to be immunoreactive to insulin and expressed pancreatic duodenal homeobox 1 ( PDX 1 ) , proinsulin 1 , proinsulin 2 , glucagon , pancreatic polypeptide , glucose transporter 2 ( GLUT 2 ) , and islet specific glucose 6 phosphatase catalytic subunit related protein ( IGRP ) mRNA . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| Real time quantitative RT PCR analysis of islets from Anx 7 heterozygous mice and littermate controls revealed remarkable down regulation in PTEN , Glut 2 , PDX 1 , IGF 1 , and Neuro D 1 expression , but not in liver . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| The authors have derived a new beta cell line ( betaIns 2 ( / lacZ ) ) from Ins 2 / mice that carry the lacZ reporter gene under control of the Ins 2 promoter . betaIns 2 ( / lacZ ) cells stained positively using anti insulin antibody , expressed beta cell specific genes encoding the transcription factor PDX 1 , glucokinase , and Glut 2 , retained glucose responsiveness for insulin secretion , and expressed the lacZ gene . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| We have also demonstrated the expression of upstream genes of Pdx 1 , such as HNF3beta and HNF1alpha , as well as its downstream genes , including insulin , Glut 2 , and Nkx6 . 1 , to be well preserved . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| PIC + IFN gamma inhibited insulin and GLUT 2 expression without modifying pdx 1 mRNA expression . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| Further more , GLP 1 upregulates expression of b cell genes ( GLUT 2 , glucokinase , insulin , and PDX 1 ) and promotes b cell neogenesis and differentiation of ductal cells into insulin secreting cells . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| Cell trapping selection yielded an almost pure population of insulin positive cells , which expressed the beta cell genes / proteins Pdx 1 , Nkx6 . 1 , insulin , glucokinase , GLUT 2 and Sur 1 . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| We observed that the number of c Kit , glucose transport type 2 ( Glut 2 ) and the transcription factor pancreatic duodenal homeobox 1 ( PDX 1 ) expressing cells were significantly increased in the HGF+ group . ^^^ Co localization of insulin and PDX 1 or Glut 2 was revealed frequently in cells treated with HGF+ with occasional co staining of c Kit and insulin observed . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| The newly formed cells secreted insulin in response to glucose and were immunoreactive for C peptide 1 , Pdx 1 and GLUT 2 , which are characteristics of mature beta cells . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| Our results show that TPCK blocked rIL 1beta mediated early increase of MnSOD activity and beta cell defence / repair protein expression , suggesting a protective role for NF kappaB at the beginning of IL 1beta treatment ; but , in a second phase , NF kappaB induces a sustained decrease of specific beta cell proteins like insulin , GLUT 2 and PDX 1 with a concomitant increase of aspecific proteins and iNOS transcription . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| Arginine stimulated insulin secretion , pancreatic insulin mRNA and peptide levels , beta cell mass , islet architecture , and GLUT 2 and PDX 1 immunoreactivity in RIP G Tg pancreas were not significantly different from those of nontransgenic littermates . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| Further analysis revealed that insulin 1 , insulin 2 , Pdx 1 , Beta 2 , and Glut 2 transcripts are diminished in MafA deficient mice . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| Although the expression of islet hormones appeared normal , CART KO islets displayed age dependent reduction of pancreatic duodenal homeobox 1 ( PDX 1 ) and glucose transporter 2 ( GLUT 2 ) immunoreactivity , indicating beta cell dysfunction . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| The release of insulin , the expression of preproinsulin ( PPI ) , glucose transporter 2 ( GLUT 2 ) and pancreatic duodenal homeobox 1 ( PDX 1 ) , and levels of intracellular free Ca++ ( [ Ca++ ] 1 ) were measured in rat pancreatic islets treated with or without high concentrations of FFA ( 0 . 1 and 1 . 0 mM oleic acid ) for 24 h . ^^^ Elevated expression of PPI , PDX 1 and GLUT 2 was also observed after treatment of the islets with oleic acid , which may partially contribute to the increased basal insulin secretion . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| A five stage culturing strategy starting with embryoid bodies ( EBs ) was used in this study . mRNA for pancreatic duodenal homeobox gene 1 ( PDX 1 ) and neurogenic differentiation ( NeuroD ) was detected from stage 1 , hepatocyte nuclear factor 3 beta ( HNF3beta ) and insulin 2 from stage 2 , Ngn 3 and glucose transporter 2 ( GLUT 2 ) from stage 3 , and insulin 1 and other beta cell markers , at stages 4 5 . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| Islet cell differentiation was studied by the following : ( a ) 10 gal staining after neomycin selection , ( b ) BrdU ( bro modeoxyuridine ) studies , ( c ) simple and double immunohistochemistry for insulin , C peptide , and glucose transporter 2 ( Glut 2 ) , ( d ) reverse transcription polymerase chain reaction for insulin and pancreas duodenum homeobox 1 ( PDX 1 ) , ( e ) insulin and C peptide content and secretion assays , ( f ) intraperitoneal glucose tolerance test , ( g ) electrophysiology ( patch clamp studies in inside out configuration ) , and ( h ) transplantation of differentiated cells under the kidney capsule of streptozotocin diabetic mice . ^^^ The differentiated ESCs showed the following : changes in the mRNA levels of insulin and PDX 1 ; coexpression of insulin , C peptide , and Glut 2 ; glucose and tolbutamide dependent insulin and C peptide release ; K channel activity regulated by ATP ; and normalization of blood glucose levels after transplantation into diabetic mice and hyperglycemia after graft removal . ^^^ |
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| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| In correlation with this degradation , we observed a subsequent reduction in the activation of the insulin promoter and a decrease in PDX 1 mediated gene expression , i . e . glut 2 and glucokinase . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| We sought to assess the capacity of hepatocyte growth factor ( HGF ) to induce expression and maturation of proteins PDX 1 and GLUT 2 and the subsequent beta cell secretory profiles . ^^^ In all conditions immunostaining studies showed positivity for the major endocrine phenotype cell markers : insulin , PDX 1 , glucokinase , and GLUT 2 . ^^^ |
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| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| The aim of the present study was to determine , in weanling Wistar rats , the effect of a maternal high fat diet ( HFD ) during defined periods of gestation and lactation , on body weight , circulating glucose and insulin concentrations , and the expression of GLUT 2 , GK and Pdx 1 . ^^^ Pancreatic sections , immunostained for GLUT 2 , GK or Pdx 1 , were assessed by image analysis . ^^^ |
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| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| Analysis of gene expression in LXR agonist treated INS 1E cells and islets from LXRbeta deficient mice revealed that LXRbeta positively regulated expression of ATP binding cassette transporter A 1 ( ABCA 1 ) , sterol regulatory element binding protein 1 ( SREBP 1 ) , insulin , PDX 1 , glucokinase , and glucose transporter 2 ( Glut 2 ) . ^^^ Down regulation of SREBP 1 expression with the specific small interfering RNA blocked basal and LXRbeta induced expression of pancreatic duodenal homeobox 1 ( PDX 1 ) , insulin , and Glut 2 genes . ^^^ |
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| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| Semi quantitative PCR indicated mRNA expression of islet development markers ( nestin , Pdx 1 , Nkx6 . 1 , Oct 4 ) , mature pancreatic beta cell markers ( insulin , glucagon , Glut 2 , Sur 1 , Kir6 . 1 ) and the GIP receptor gene GIP R in undifferentiated ( stage 1 ) cells , with increasing levels in differentiated stages 4 and 5 . ^^^ |
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| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| RNA of Pdx 1 , Glut 2 , and Gck was detectable by RT PCR in whole thymus but not in the clones , suggesting thymic proinsulin expression is Pdx 1 independent and that Pdx 1 , Glut 2 , and Gck are likely expressed in the thymus as antigens , not as regulatory molecules . . ^^^ |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11168 and P52945 |
Pubmed |
SVM Score :0.0 |
| NA |
|