| Interacting proteins: P10997 and P52945 |
Pubmed |
SVM Score :0.0 |
| The gene encoding amylin is implicated in the generation of amyloid in the islets of Langerhans of diabetics and is believed to be regulated by the homeodomain transcription factor PDX 1 . ^^^ Studies of PDX 1 deficient mice , which fail to develop gastrin cells while possessing normal numbers of somatostatin cells , revealed no detectable change in gastric amylin expression . ^^^ These data show that neither normal gastrin cell development nor PDX 1 expression is needed for gastric amylin expression . . ^^^ |
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| Interacting proteins: P10997 and P52945 |
Pubmed |
SVM Score :0.0 |
| By exogenous expression of the PDX 1 gene , IEC 6 cells started expressing multiple beta cell specific genes such as amylin , glucokinase , and Nkx6 . 1 , which were not found in the original IEC 6 cells . ^^^ |
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| Interacting proteins: P10997 and P52945 |
Pubmed |
SVM Score :0.0 |
| Moreover , Ex 4 increased the levels of insulin and amylin mRNA transcripts and augmented glucose stimulated insulin secretion in islets from beta cell ( Pdx1+ / + ) mice but not in beta cell ( Pdx 1 / ) islets . ^^^ |
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| Interacting proteins: P10997 and P52945 |
Pubmed |
SVM Score :0.0 |
| We could not find any mutations in HNF 1alpha , 1beta , 4alpha , IPF 1 , ISL 1 , BEAT2 / NeuroD1 , PAX 4 , and amylin genes . ^^^ |
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| Interacting proteins: P10997 and P52945 |
Pubmed |
SVM Score :0.0 |
| In islet cell tumour models we found that a glucagon / islet amyloid polypeptide ( IAPP ) producing pluripotent rat islet cell line ( NHI 6F GLU ) expresses STF 1 in all cells prior to insulin gene activation induced by in vivo culture . ^^^ |
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| Interacting proteins: P10997 and P52945 |
Pubmed |
SVM Score :0.0 |
| This complex was displaced with an antiserum to IUF 1 , confirming that IUF 1 binds to the human IAPP promoter in vitro . ^^^ |
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| Interacting proteins: P10997 and P52945 |
Pubmed |
SVM Score :0.0 |
| We find that in addition to insulin the insulinoma express the normal beta cell markers Pdx 1 , IAPP , and Glut 2 , and that these markers are absent from the glucagonoma : a reflection of the normal alpha cell . ^^^ |
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| Interacting proteins: P10997 and P52945 |
Pubmed |
SVM Score :0.0 |
| The exogenous expression of PDX 1 in alphaTC1 . 6 cells alone could induce islet amyloid polypeptide ( IAPP ) mRNA expression in the cells but not the expression of insulin , glucokinase , or GLUT 2 gene . ^^^ These observations demonstrate the potency of PDX 1 for the expression of the insulin , glucokinase , and IAPP genes and suggest that certain regulatory factors , which can partially be modified by betacellulin , also contribute to the beta cell specificity of gene expression . . ^^^ |
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| Interacting proteins: P10997 and P52945 |
Pubmed |
SVM Score :0.0 |
| These observations suggest the involvement of PDX 1 in human IAPP gene regulation , which seems to be mediated through at least two A element like cis motifs in the gene promoter . . ^^^ |
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| Interacting proteins: P10997 and P52945 |
Pubmed |
SVM Score :0.0 |
| Since PDX 1 is highly enriched in beta and delta cells , these results suggest that this factor plays a principal role in defining islet beta cell and delta cell specific expression of the IAPP gene . . ^^^ |
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| Interacting proteins: P10997 and P52945 |
Pubmed |
SVM Score :0.0 |
| Forced expression of recombinant PDX 1 in the glucagonoma resulted in efficient transcriptional activation of the endogenous insulin and IAPP genes , but did not affect glucagon gene activity . ^^^ We conclude that PDX 1 in synergy with NeuroD specifies part of the beta cell phenotype including transcriptional activation of insulin and IAPP genes , but that other factors such as Nkx6 . 1 and Pax 6 are required for additional features of the fully mature beta cell phenotype . . ^^^ |
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| Interacting proteins: P10997 and P52945 |
Pubmed |
SVM Score :0.0 |
| Here we examine the role of PDX 1 in the regulation of the human IAPP promoter by glucose . ^^^ Overexpression of PDX 1 in NES2Y cells failed to reinstate glucose responsive control of the IAPP promoter . ^^^ Glucose effects on the IAPP promoter were observed only in the presence of PDX 1 when normal calcium signaling was restored by overexpression of the two K ( ATP ) channel subunits SUR 1 and Kir6 . 2 . ^^^ These results demonstrate that like the insulin promoter , glucose regulation of the IAPP promoter is dependent on the activity of PDX 1 , but unlike the insulin promoter , it additionally requires the activity of another , as yet uncharacterized factor ( s ) , the activity of which is calcium dependent . . ^^^ |
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| Interacting proteins: P10997 and P52945 |
Pubmed |
SVM Score :0.0 |
| This promoter region contains sequence motifs that have been shown to be involved in beta cell specific expression of insulin , Pdx 1 and islet amyloid polypeptide ( IAPP ) . ^^^ |
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| Interacting proteins: P10997 and P52945 |
Pubmed |
SVM Score :0.0 |
| GLUT 2 mRNA was decreased , but other beta cell associated genes ( IAPP [ islet amyloid pancreatic polypeptide ] , PDX 1 [ pancreatic and duodenal homeobox 1 ] , and BETA2 / NeuroD ) were expressed at near normal levels . ^^^ |
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| Interacting proteins: P10997 and P52945 |
Pubmed |
SVM Score :0.0 |
| Hormone positive cells appeared within focal clusters of cells coexpressing PDX 1 and the nonclassical hormone markers peptide YY ( YY ) and islet amyloid polypeptide ( IAPP ) in combination with the definitive hormones , characteristic of endocrine cells appearing during early pancreaticogenesis . ^^^ |
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| Interacting proteins: P10997 and P52945 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10997 and P52945 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10997 and P52945 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10997 and P52945 |
Pubmed |
SVM Score :0.0 |
| NA |
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