| Interacting proteins: P52597 and P31943 |
Pubmed |
SVM Score :1.0293018 |
| Finally , in vitro binding assays demonstrate that hnRNP H can interact with the related protein hnRNP F , suggesting that hnRNPs H and F may exist as a heterodimer in a single enhancer complex . 1.0293018^^^ |
|
| Interacting proteins: P52597 and P31943 |
Pubmed |
SVM Score :0.0 |
| The arrangement of hnRNP proteins on pre mRNAs is likely to be unique for each RNA and may be determined by the different RNA binding preferences of each of these proteins . hnRNP F ( M ( r ) = 53 kD , pI = 6 . 1 ) and hnRNP H ( M ( r ) = 56 kD , pI = 6 . 7 7 . 1 ) are abundant components of immunopurified hnRNP complexes and they have distinct nucleic acid binding properties . ^^^ Unlike other hnRNP proteins which display a varying range of affinities for different ribonucleotidehomopolymers and ssDNA , hnRNP F and hnRNP H bind only to poly ( rG ) in vitro . hnRNP F and hnRNP H were purified from HeLa cells by poly ( rG ) affinity chromatography and oligonucleotides derived from peptide sequences were used to isolate a cDNA encoding hnRNP F . ^^^ Monoclonal antibodies produced against bacterially expressed hnRNP F were specific for both hnRNP F and hnRNP H and recognized related proteins in divergent organisms , including in the yeast Saccharomyces cerevisiae . hnRNP F and hnRNP H are thus highly related immunologically and they share identical peptides . ^^^ Interestingly , immunofluorescence microscopy revealed that hnRNP F and hnRNP H are concentrated in discrete regions of the nucleoplasm , in contrast to the general nucleoplasmic distribution of previously characterized hnRNP proteins . ^^^ The unique RNA binding properties , amino acid sequence and distinct intranuclear localization of hnRNP F and hnRNP H make them novel hnRNP proteins that are likely to be important for the processing of RNAs containing guanosine rich sequences . . ^^^ |
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| Interacting proteins: P52597 and P31943 |
Pubmed |
SVM Score :0.0 |
| The hnRNP H protein , which is 78 % identical to hnRNP F and also interacts with both CBP 80 and CBP 20 in vitro , does not discriminate between naked RNA and CBC RNA complexes , showing that this effect is specific . ^^^ |
|
| Interacting proteins: P52597 and P31943 |
Pubmed |
SVM Score :0.0 |
| The c src DCS has been shown to assemble hnRNP H , not hnRNP F , from HeLa cell extracts , and we show that hnRNP 2H9 does not bind this element . ^^^ |
|
| Interacting proteins: P52597 and P31943 |
Pubmed |
SVM Score :0.0 |
| Proteins binding to this element were identified as hnRNP A 1 , hnRNP H , hnRNP F , and SF2 / ASF by site specific cross linking and immunoprecipitation . ^^^ |
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| Interacting proteins: P52597 and P31943 |
Pubmed |
SVM Score :0.0 |
| It is further shown that hnRNP H ( but not hnRNP F ) can promote U 11 binding and splicing from the NRS in vivo when tethered to the RNA as an MS 2 fusion protein . ^^^ |
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| Interacting proteins: P52597 and P31943 |
Pubmed |
SVM Score :0.0 |
| In order to gain insight into G tract recognition by hnRNP H members , we initiated an NMR study of human hnRNP F . ^^^ |
|
| Interacting proteins: P52597 and P31943 |
Pubmed |
SVM Score :0.0 |
| The genes coding for hnRNPs H , H ' , and F were chromosome mapped to 5q35 . 3 ( HNRPH 1 ) , 6q25 . 3 q 26 , and / or Xq 22 ( HNRPH 2 ) and 10q11 . 21 q11 . 22 ( HNRPF ) , respectively . . ^^^ |
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| Interacting proteins: P52597 and P31943 |
Pubmed |
SVM Score :0.0 |
| NA |
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