| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.52917475 |
| The purified p38alpha protein was efficiently activated by MKK 6 kinase to yield phosphorylated p38alpha . 0.52917475^^^ |
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| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| Activation of stress activated protein kinase 3 ( SAPK 3 ) by cytokines and cellular stresses is mediated via SAPKK 3 ( MKK 6 ) ; comparison of the specificities of SAPK 3 and SAPK 2 ( RK / p38 ) . ^^^ |
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| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| MEK 6 regulates human involucrin gene expression via a p38alpha and p38delta dependent mechanism . ^^^ These results suggest that MEK 6 increases hINV gene expression by regulating the balance between activation of p38alpha , which increases gene expression , and p38delta , which decreases gene expression . . ^^^ |
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| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| To evaluate the potential inhibitory role of p 38 more directly , cells were dialyzed with recombinant p38alpha and its upstream activator , MEK 6 , which substantially inhibited volume sensitive currents . ^^^ |
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| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| We also observed that p38delta was strongly activated by MKK 3 and MKK 6 , while p38alpha was preferentially activated by MKK 6 . ^^^ |
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| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| The p 38 MAP kinase kinase MKK 6 is identified as a common activator of p 38 alpha , p 38 beta 2 , and p 38 gamma MAP kinase isoforms , while MKK 3 activates only p 38 alpha and p 38 gamma MAP kinase isoforms . ^^^ |
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| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| Upstream activators of p 38 , constitutively active ( ca ) MKK 3 and MKK 6 , were also cloned , purified , and used to activate each p 38 isoform . p 38 alpha , gamma , and delta were phosphorylated by both MKK 6 and caMKK 3 . p38beta was phosphorylated only by MKK 6 . ^^^ |
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| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| Additionally , we demonstrated that these GPCRs can elevate the activity of novel members of the MAPK family , including ERK 5 , p38alpha , p38gamma , and p38delta , and that the activation of certain kinases acting downstream from MEK 5 ( ERK 5 ) and MKK 6 ( p38alpha and p38gamma ) is necessary to fully activate the c jun promoter . ^^^ |
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| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| MKK 6 autophosphorylation and in vitro activation of p38alpha were also observed following coexpression of MKK 6 with MEKK 3 . ^^^ |
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| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| In these primary human cells , CsA significantly inhibited PMA / ionomycin mediated and ionomycin mediated activation of the MAPK kinase MKK 6 , as well as its downstream kinases SAPK2a ( p38alpha ) and MAPKAP K 2 . ^^^ |
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| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| Co expression of the dominant negative forms of MKK 4 , MKK 7 , JNK / SAPK , MKK 3 , MKK 6 , or p38alpha did not suppress PMA stimulated reporter gene expression . ^^^ |
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| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| Moreover , we demonstrate that p38alpha and its upstream activator , MKK 6 , phosphorylate STAT 4 on serine 721 , and are required for STAT 4 full transcriptional activity induced by IL 12 , establishing the MKK6 / p38alpha / STAT4 pathway as an important mediator of IL 12 actions . ( Blood . 2000 ; 96 : 1844 1852 ) . ^^^ |
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| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| We have investigated the ability of the mitogen activated protein kinase ( MAPK ) kinase MKK 6 to activate different members of the p 38 subfamily of MAPKs and found that some MKK 6 mutants can efficiently activate p38alpha but not p38gamma . ^^^ We also found that the preferential activation of p38alpha by MKK 6 correlated with more efficient binding of MKK 6 to p38alpha than to p38gamma . ^^^ Furthermore , increasing concentrations of constitutively active MKK 6 differentially activated either p38alpha alone ( low MKK 6 activity ) or both p38alpha and p38gamma ( high MKK 6 activity ) , both in vitro and in injected oocytes . ^^^ |
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| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| The loss of epidermal growth factor induced activation and phosphorylation of ERK1 / 2 but not of their direct activator MEK 1 in HeLa cells transfected with the p38alpha activator MKK 6 ( E ) indicated that activated p38alpha may sequester ERK1 / 2 and sterically block their phosphorylation by MEK1 . . ^^^ |
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| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| Conversely , active MKK 3 and MKK 6 , both of which increase p38alpha activity , each decreased transcription from the cyclin D 1 promoter . ^^^ |
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| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| Even though Exip is not phosphorylated at conserved TGY motif by p 38 activating treatments , such as an osmotic shock or coexpression with a constitutive active form of MKK 6 in HeLa cells , Exip can induce an earlier onset of apoptosis in HeLa cells . ^^^ |
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| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| Negative feedback regulation of MKK 6 mRNA stability by p38alpha mitogen activated protein kinase . p 38 mitogen activated protein ( MAP ) kinases play an important role in the regulation of cellular responses to all kinds of stresses . ^^^ Here we show that the absence of p38alpha correlates with the up regulation of one of its upstream activators , the MAP kinase kinase MKK 6 , in p38alpha ( / ) knockout mice and in cultured cells derived from them . ^^^ The increase in MKK 6 protein concentration correlates with increased amounts of MKK 6 mRNA in the p38alpha ( / ) cells . ^^^ Pharmacological inhibition of p38alpha also up regulates MKK 6 mRNA levels in HEK 293 cells . ^^^ Conversely , reintroduction of p38alpha into p38alpha ( / ) cells reduces the levels of MKK 6 protein and mRNA to the normal levels found in wild type cells . ^^^ |
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| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| Here we generated cardiac specific transgenic mice expressing dominant negative mutants of p38alpha , MKK 3 , or MKK 6 . ^^^ Moreover , dominant negative p38alpha , MKK 3 , and MKK 6 transgenic mice each showed enhanced cardiac hypertrophy following aortic banding , Ang 2 infusion , isoproterenol infusion , or phenylephrine infusion for 14 days . ^^^ |
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| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| In vivo , transgenic mice expressing a dominant negative MKK 6 mutant or a dominant negative p38alpha mutant were each significantly protected from ischemia reperfusion injury , as assessed by infarct area measurements , DNA laddering , terminal deoxynucleotidyltransferase mediated dUTP nick end labeling , and functional assessment of ventricular performance . ^^^ |
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| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| Overexpression of dominant negative p 38 isoforms and activated MKK 3 and MKK 6 in isolated adult cardiac myocytes demonstrates that p 38 activation is sufficient for NCX 1 promoter upregulation and that this is mediated primarily by the p38alpha isoform . ^^^ |
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| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| Cells deficient in Mkk 3 and Mkk 6 , the upstream regulators of p38alpha , also fail to activate HIF 1 under hypoxic conditions . ^^^ |
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| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| Prevention of MKK 6 dependent activation by binding to p38alpha MAP kinase . ^^^ MKK 6 dependent phosphorylation on the activation loop of p38alpha increases its catalytic activity and affinity for ATP . ^^^ We now demonstrate that selectivity pocket compounds prevent MKK 6 dependent activation of p38alpha in addition to inhibiting catalysis by activated p38alpha . ^^^ Stabilization of a DFG out conformation appears to interfere with recognition of p38alpha as a substrate by MKK 6 . ^^^ |
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| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52564 and Q16539 |
Pubmed |
SVM Score :0.0 |
| NA |
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