| Interacting proteins: P52435 and P24928 |
Pubmed |
SVM Score :0.0 |
| Far Western blot analysis of S . pombe RNA polymerase 2 using 32P labelled recombinant Rpb 5 fused to glutathione S transferase ( GST ) as a probe , indicated that Rpb 5 binds strongly to membrane immobilized Rpb 1 , Rpb 2 and Rpb 3 and weakly to Rpb 5 and a 15 kDa subunit ( Rpb 8 or Rpb 11 ) . ^^^ |
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| Interacting proteins: P52435 and P24928 |
Pubmed |
SVM Score :0.0 |
| After stepwise dissociation of the Rpb 1 and Rpb 3 tagged RNA polymerases fixed on Ni2+ resin by increasing concentrations of urea or guanidium hydrochloride , Rpb 2 Rpb3 Rpb 11 or Rpb 2 Rpb3 Rpb 11 Rpb10 complexes were obtained . ^^^ Both the Rpb 2 Rpb3 Rpb 11 complex and the free Rpb 1 protein showed DNA binding activity , although the affinity was weaker compared with the intact RNA polymerase . . ^^^ |
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| Interacting proteins: P52435 and P24928 |
Pubmed |
SVM Score :0.0 |
| Previously we indicated that Rpb 3 also plays an essential role in subunit assembly because it interacts with at least four subunits , two large subunits ( Rpb 1 and Rpb 2 ) and two medium sized subunits ( Rpb 3 and Rpb 5 ) ( 1 ) , and it constitutes a core subassembly consisting of Rpb 2 , Rpb 3 , and Rpb 11 ( 2 ) . ^^^ |
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| Interacting proteins: P52435 and P24928 |
Pubmed |
SVM Score :0.0 |
| Coexpression of various combinations of a few subunits revealed that Rpb 11 enhances Rpb 3 Rpb8 interaction and consequently Rpb 8 enhances Rpb 1 Rpb3 interaction to some extent . ^^^ |
|
| Interacting proteins: P52435 and P24928 |
Pubmed |
SVM Score :0.0 |
| The transcription of one group of genes including rpb 3 , rpb 4 , rpb 5 , rpb 6 , rpb 7 and rpb 10 is mainly initiated at a single site , while that of the other group of genes for rpb 1 , rpb 2 , rpb 8 , rpb 9 , rpb 11 and rpb 12 is initiated at multiple sites . ^^^ The protein to mRNA ratio or the translation efficiency is low for the rpb 1 , rpb 2 , rpb 3 and rpb 11 genes , encoding the homologues of subunits beta ' , beta , alpha and alpha , respectively , of the prokaryotic RNA polymerase core enzyme . . ^^^ |
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| Interacting proteins: P52435 and P24928 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : The RNA polymerase 2 of the fission yeast Schizosaccharomyces pombe consists of 12 Rpb subunits , of which four ( Rpb 1 , Rpb 2 , Rpb 3 and Rpb 11 ) form the assembly and catalytic core and five ( Rpb 5 , Rpb 6 , Rpb 8 , Rpb 10 and Rpb 12 ) are shared among RNA polymerases 1 , 2 and 3 . ^^^ |
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| Interacting proteins: P52435 and P24928 |
Pubmed |
SVM Score :0.0 |
| Nine subunits homologous to the other eukaryotic RNA Pol 2 , namely RPB 1 , RPB 2 , RPB 3 , RPB 4 , RPB 5 , RPB 6 , RPB 7 , RPB 8 and RPB 11 , were identified in the purified complex . ^^^ |
|
| Interacting proteins: P52435 and P24928 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52435 and P24928 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52435 and P24928 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P52435 and P24928 |
Pubmed |
SVM Score :0.0 |
| NA |
|