| Interacting proteins: P52435 and P19387 |
Pubmed |
SVM Score :0.0 |
| The aa sequence of hRPB 14 has homology to yeast poIII subunit RPB 11 ( 44 % ) , to a common subunit of yeast polI and polIII AC 19 ( 24 % ) and to a Caenorhabditis elegans sequence ( 33 % ) . hRPB 14 contains a 19 aa motif , located in its N terminus , which was also found in human polII 33 kDa subunit hRPB 33 , yeast pol subunits ( AC 40 , AC 19 , RPB 3 and RPB 11 ) , and in the bacterial pol alpha subunit , which was involved in subunit assembly . ^^^ |
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| Interacting proteins: P52435 and P19387 |
Pubmed |
SVM Score :0.0 |
| RPB 3 and RPB 11 in yeast ) and two subunits common to RNA polymerases 1 and 3 ( e . g . ^^^ We have sequenced two different cDNAs ( AtRPB36a and AtRPB36b ) , the two corresponding genes from Arabidopsis thaliana that are homologs of yeast RPB 3 , and an Arabidopsis cDNA ( AtRPB13 . 6 ) that is a homolog of yeast RPB 11 . ^^^ |
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| Interacting proteins: P52435 and P19387 |
Pubmed |
SVM Score :0.0 |
| Far Western blot analysis of S . pombe RNA polymerase 2 using 32P labelled recombinant Rpb 5 fused to glutathione S transferase ( GST ) as a probe , indicated that Rpb 5 binds strongly to membrane immobilized Rpb 1 , Rpb 2 and Rpb 3 and weakly to Rpb 5 and a 15 kDa subunit ( Rpb 8 or Rpb 11 ) . ^^^ |
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| Interacting proteins: P52435 and P19387 |
Pubmed |
SVM Score :0.0 |
| After stepwise dissociation of the Rpb 1 and Rpb 3 tagged RNA polymerases fixed on Ni2+ resin by increasing concentrations of urea or guanidium hydrochloride , Rpb 2 Rpb3 Rpb 11 or Rpb 2 Rpb3 Rpb 11 Rpb10 complexes were obtained . ^^^ Since the complex consisting of Rpb 2 , Rpb 3 , and Rpb 11 can not be dissociated even after treatment with 6 M urea buffer , we propose that this complex represents a core subassembly of the RNA polymerase 2 , analogous to the alpha2beta complex in the assembly of Escherichia coli RNA polymerase . ^^^ |
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| Interacting proteins: P52435 and P19387 |
Pubmed |
SVM Score :0.0 |
| Previously we indicated that Rpb 3 also plays an essential role in subunit assembly because it interacts with at least four subunits , two large subunits ( Rpb 1 and Rpb 2 ) and two medium sized subunits ( Rpb 3 and Rpb 5 ) ( 1 ) , and it constitutes a core subassembly consisting of Rpb 2 , Rpb 3 , and Rpb 11 ( 2 ) . ^^^ Using a synthetic mixture of equimolar amounts of individual subunits , which were all purified from cDNA expressed Escherichia coli , we found here that Rpb 3 also interacts with Rpb 11 , another alpha homologue . ^^^ By making a set of Rpb 3 deletion derivatives , we carried out mapping of the Rpb 5 and Rpb 11 contact sites on Rpb 3 . ^^^ By far Western blot and GST pull down assays , we found that the amino acid sequence between residues 105 263 of Rpb 3 is involved in binding Rpb 5 , and the sequence between residues 105 297 is required for binding Rpb 11 . ^^^ Although the Rpb 5 and Rpb 11 contact sites on Rpb 3 overlap each other , both subunits are able to associate with Rpb 3 simultaneously . ^^^ |
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| Interacting proteins: P52435 and P19387 |
Pubmed |
SVM Score :0.0 |
| Sequence alignments , combined with secondary structure predictions , support proposals that a heterodimer of the eukaryotic RNAP subunits related to Saccharomyces cerevisiae Rpb 3 and Rpb 11 plays the role of the alphaNTD dimer in prokaryotic RNAP . . ^^^ |
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| Interacting proteins: P52435 and P19387 |
Pubmed |
SVM Score :0.0 |
| Previously we demonstrated that the Schizosaccharomyces pombe Rpb 3 protein forms a core subassembly together with Rpb 2 ( the beta homologue ) and Rpb 11 ( the second alpha homologue ) subunits , as in the case of the prokaryotic alpha2beta complex . ^^^ In accordance with this prediction , the Ts phenotype of all the mutants was suppressed to varying extents by overexpression of Rpb 11 , the pairing partner of Rpb 3 in the core subassembly . ^^^ |
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| Interacting proteins: P52435 and P19387 |
Pubmed |
SVM Score :0.0 |
| After successive affinity chromatography on glutathione and Ni ( 2+ ) columns , complexes consisting of the seven subunits , Rpb1H , Rpb 2 , GST Rpb 3 , Rpb 5 , Rpb 7 , Rpb 8 and Rpb 11 , were identified . ^^^ Omission of the GST Rpb 3 expression resulted in reduced assembly of the Rpb 11 into the complex . ^^^ Coexpression of various combinations of a few subunits revealed that Rpb 11 enhances Rpb 3 Rpb8 interaction and consequently Rpb 8 enhances Rpb 1 Rpb3 interaction to some extent . ^^^ |
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| Interacting proteins: P52435 and P19387 |
Pubmed |
SVM Score :0.0 |
| The two alpha like subunits ( Rpb 3 and Rpb 11 ) , however , did not support growth at high ( 37 degrees C ) or low ( 25 degrees C ) temperatures . ^^^ In the case of Rpb 3 , growth was restored by increasing the gene dosage of the host Rpb 11 or Rpb 10 subunits , confirming previous evidence of a close genetic interaction between these three subunits . . ^^^ |
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| Interacting proteins: P52435 and P19387 |
Pubmed |
SVM Score :0.0 |
| The transcription of one group of genes including rpb 3 , rpb 4 , rpb 5 , rpb 6 , rpb 7 and rpb 10 is mainly initiated at a single site , while that of the other group of genes for rpb 1 , rpb 2 , rpb 8 , rpb 9 , rpb 11 and rpb 12 is initiated at multiple sites . ^^^ The protein to mRNA ratio or the translation efficiency is low for the rpb 1 , rpb 2 , rpb 3 and rpb 11 genes , encoding the homologues of subunits beta ' , beta , alpha and alpha , respectively , of the prokaryotic RNA polymerase core enzyme . . ^^^ |
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| Interacting proteins: P52435 and P19387 |
Pubmed |
SVM Score :0.0 |
| Together with the Rpb 2 ( the beta homologue ) and Rpb 11 ( the second alpha homologue ) subunits , Rpb 3 constitutes a core subassembly ( Rpb 2 Rpb3 Rpb 11 ) which corresponds to the the alpha2beta assembly intermediate of prokaryotic RNA polymerase . ^^^ |
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| Interacting proteins: P52435 and P19387 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : The RNA polymerase 2 of the fission yeast Schizosaccharomyces pombe consists of 12 Rpb subunits , of which four ( Rpb 1 , Rpb 2 , Rpb 3 and Rpb 11 ) form the assembly and catalytic core and five ( Rpb 5 , Rpb 6 , Rpb 8 , Rpb 10 and Rpb 12 ) are shared among RNA polymerases 1 , 2 and 3 . ^^^ |
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| Interacting proteins: P52435 and P19387 |
Pubmed |
SVM Score :0.0 |
| RPB 3 is a core subunit of RNA polymerase 2 ( pol 2 ) that , together with the RPB 11 subunit , forms the heterodimer considered as a functional counterpart of the bacterial alpha subunit homodimer involved in promoter recognition . ^^^ |
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| Interacting proteins: P52435 and P19387 |
Pubmed |
SVM Score :0.0 |
| Distinct regions of RPB 11 are required for heterodimerization with RPB 3 in human and yeast RNA polymerase 2 . ^^^ In contrast , the human heterodimer tolerates significant changes in RPB 11 C terminus , allowing two human RPB 11 variants to heterodimerize with the same efficiency with RPB 3 . ^^^ |
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| Interacting proteins: P52435 and P19387 |
Pubmed |
SVM Score :0.0 |
| A selection for trans acting mutations that induce readthrough of both a snoRNA and an mRNA terminator yielded mutations in the Rpb 3 and Rpb 11 subunits of Pol 2 that define a remarkably discrete surface on the trailing end of the enzyme . ^^^ |
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| Interacting proteins: P52435 and P19387 |
Pubmed |
SVM Score :0.0 |
| Nine subunits homologous to the other eukaryotic RNA Pol 2 , namely RPB 1 , RPB 2 , RPB 3 , RPB 4 , RPB 5 , RPB 6 , RPB 7 , RPB 8 and RPB 11 , were identified in the purified complex . ^^^ |
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| Interacting proteins: P52435 and P19387 |
Pubmed |
SVM Score :0.0 |
| Homology searches further revealed that P 35 has structural similarity with RPB 3 , the subunit of RNA polymerase 2 that has been demonstrated to interact directly with RPB11a . ^^^ |
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| Interacting proteins: P52435 and P19387 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P52435 and P19387 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P52435 and P19387 |
Pubmed |
SVM Score :0.0 |
| NA |
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